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| 1 | A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro显示文摘Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain diseases.Although there have been several studies related to ultrasonic neuromodulation,these studies have suffered from poor spatial resolution of the ultrasound and low repeatability with a fixed condition caused by conventional and commercialized ultrasound transducers.In addition,the underlying physics and mechanisms of ultrasonic neuromodulation are still unknown.To determine these mechanisms and accurately modulate neural circuits,researchers must have a precisely controllable ultrasound transducer to conduct experiments at the cellular level.Herein,we introduce a new MEMS ultrasound stimulation system for modulating neurons or brain slices with high spatial resolution.The piezoelectric micromachined ultrasonic transducers(pMUTs)with small membranes(submm membranes)generate enough power to stimulate neurons and enable precise modulation of neural circuits.We designed the ultrasound transducer as an array structure to enable localized modulation in the target region.In addition,we integrated a cell culture chamber with the system to make it compatible with conventional cell-based experiments,such as in vitro cell cultures and brain slices.In this work,we successfully demonstrated the functionality of the system by showing that the number of responding cells is proportional to the acoustic intensity of the applied ultrasound.We also demonstrated localized stimulation capability with high spatial resolution by conducting experiments in which cocultured cells responded only around a working transducer. | Jungpyo Lee Kyungmin Ko Hyogeun Shin Soo-Jin Oh CJustin Lee Namsun Chou Nakwon Choi Min Tack Oh Byung Chul Lee Seong Chan Jun Il-Joo Cho | 2019 | Microsystems & Nanoengineering2019，5，1： | 3 |
| 2 | The effect of ambient pressure on the evaporation of a single droplet and a spray显示文摘 | Hongsuk Kim Nakwon Sung | 2003 | Combustion and Flame2003，，3： | 2 |
| 3 | The Effect of Ambient Pressure on the Evaporation of a Single Droplet and a Spray显示文摘 | HONGSUK KIM NAKWON SLING | 2003 | Combustion and Flame2003，135，3： | 1 |
| 4 | Brain physiome:A concept bridging in vitro 3D brain models and in silico models for predicting drug toxicity in the brain显示文摘In the last few decades,adverse reactions to pharmaceuticals have been evaluated using 2D in vitro models and animal models.However,with increasing computational power,and as the key drivers of cellular behavior have been identified,in silico models have emerged.These models are time-efficient and cost-effective,but the prediction of adverse reactions to unknown drugs using these models requires relevant experimental input.Accordingly,the physiome concept has emerged to bridge experimental datasets with in silico models.The brain physiome describes the systemic interactions of its components,which are organized into a multilevel hierarchy.Because of the limitations in obtaining experimental data corresponding to each physiome component from 2D in vitro models and animal models,3D in vitro brain models,including brain organoids and brain-on-a-chip,have been developed.In this review,we present the concept of the brain physiome and its hierarchical organization,including cell-and tissue-level organizations.We also summarize recently developed 3D in vitro brain models and link them with the elements of the brain physiome as a guideline for dataset collection.The connection between in vitro 3D brain models and in silico modeling will lead to the establishment of cost-effective and time-efficient in silico models for the prediction of the safety of unknown drugs. | Yoojin Seo Seokyoung Bang Jeongtae Son Dongsup Kim Yong Jeong Pilnam Kim Jihun Yang Joon-Ho Eom Nakwon Choi Hong Nam Kim | 2022 | Bioactive Materials2022，7，7： | 1 |
| 5 | KDM3B Is the H3K9 Demethylase Involved in Transcriptional Activation of lmo2 in Leukemia显示文摘 | Ji-Young Kim Kee-Beom Kim Gwang Hyeon Eom Nakwon Choe Hae Jin Kee Hye-Ju Son Si-Taek Oh Dong-Wook Kim Jhang Ho Pak Hee Jo Baek Hoon Kook Yoonsoo Hahn Hyun Kook Debabrata Chakravarti Sang-Beom Seo | 2012 | Molecular and Cellular Biology2012，，14： | 1 |
| 6 | The effect of ambient pressure on the evaporation of a single droplet and a spray 显示文摘 | HONGSUK K NAKWON S | 2003 | Combustion and Flame2003，135，： | 1 |
| 7 | The effect of ambient pressure on the evaporation of a single droplet and a spray显示文摘 | Hongsuk K Nakwon S | 2003 | Combustion and Flame2003，135，： | 1 |
| 8 | The effect of ambient pressure on the evaporation of a single droplet and a spray 显示文摘 | Hongsuk K Nakwon S | 2003 | Combustion and Flame2003，135，7： | 1 |
| 9 | The effect of ambient pressure on the evaporation of a single droplet and a spray显示文摘 | Hongsuk Kim Nakwon Sung | 2003 | Combustion and Flame2003，，3： | 1 |
| 10 | 3D engineered tissue models for studying human-specific infectious viral diseases显示文摘Viral infections cause damage to various organ systems by inducing organ-specific symptoms or systemic multi-organ damage.Depending on the infection route and virus type,infectious diseases are classified as respiratory,nervous,immune,digestive,or skin infections.Since these infectious diseases can widely spread in the com-munity and their catastrophic effects are severe,identification of their causative agent and mechanisms un-derlying their pathogenesis is an urgent necessity.Although infection-associated mechanisms have been studied in two-dimensional(2D)cell culture models and animal models,they have shown limitations in organ-specific or human-associated pathogenesis,and the development of a human-organ-mimetic system is required.Recently,three-dimensional(3D)engineered tissue models,which can present human organ-like physiology in terms of the 3D structure,utilization of human-originated cells,recapitulation of physiological stimuli,and tight cell–cell interactions,were developed.Furthermore,recent studies have shown that these models can recapitulate infection-associated pathologies.In this review,we summarized the recent advances in 3D engineered tissue models that mimic organ-specific viral infections.First,we briefly described the limitations of the current 2D and animal models in recapitulating human-specific viral infection pathology.Next,we provided an overview of recently reported viral infection models,focusing particularly on organ-specific infection pathologies.Finally,a future perspective that must be pursued to reconstitute more human-specific infectious diseases is presented. | Kyeong Seob Hwang Eun U Seo Nakwon Choi Jongbaeg Kim Hong Nam Kim | 2023 | Bioactive Materials2023，，3： | 0 |
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