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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Frequency and prognostic role of mucosal healing in patients with Crohn's disease and ulcerative colitis after one-year of biological therapy显示文摘AIM:To assess the endoscopic activity before and after a one-year period of biological therapy and to evaluate the frequency of relapses and need for retreatment after stopping the biologicals in patients with Crohn’s disease(CD)and ulcerative colitis(UC).METHODS:The data from 41 patients with CD and 22 patients with UC were assessed.Twenty-four CD patients received infliximab,and 17 received adalimumab.The endoscopic severity of CD was quantified with the simplified endoscopic activity score for Crohn’s disease in CD and with the Mayo endoscopic subscore in UC.RESULTS:Mucosal healing was achieved in 23 CD and7 UC patients.Biological therapy had to be restarted in78%of patients achieving complete mucosal healing with CD and in 100%of patients with UC.Neither clinical remission nor mucosal healing was associated with the time to restarting the biological therapy in either CD or UC.CONCLUSION:Mucosal healing did not predict sustained clinical remission in patients in whom the biological therapies had been stopped. | Klaudia Farkas Péter László Lakatos Mónika Szcs va Pallagi-Kunstár Anita Bálint Ferenc Nagy Zoltán Szepes Noémi Vass Lajos S Kiss Tibor Wittmann Tamás Molnár | 2014 | World Journal of Gastroenterology2014，20，11： | 2 |
| 3 | Anti-tumor necrosis factor-αinduced systemic lupus erythematosus in a patient with metastaticCrohn's disease-what is the role of anti-TNF antibody?显示文摘 | Farkas K Nagy F Kovács L | 2012 | J Crohn''sColitis2012，，： | 1 |
| 4 | Treatment of pituitary tumors显示文摘 | Gabriella Iván Nikoletta Szigeti-Csúcs Márk Oláh Cy?rgy M. Nagy Miklós I. Góth | 2005 | Endocrine2005，，1： | 1 |
| 5 | Hypoxia and hypoxia-induciblefactor-1 target genes in central nervous system radiation injury:a rolefor vascular endothelial growth factor显示文摘 | Nordal RA Nagy A Wong CS | 2004 | Clin Cancer Res2004，10，10： | 1 |
| 6 | Axillary sentinel lymph nodes can be falsely positive due to iatrogenic displacement and transport of benign epithelial cells in patients with breast carcinoma显示文摘 | Bleiweiss IJ Nagi CS Jaffer S | 2006 | J Clin Oncol2006，24，13： | 1 |
| 7 | Li Pid,haemo-staticand in flammatory variables in relation to the es-trogen receptor alpha (ESR) Pvu Ⅱ and XbaI gene pol-ymorphisms显示文摘 | Movarec A Nagy B Kovács M | | 0，，1： | 1 |
| 8 | Axillary sentinel lymph nodes can be falsely positive due to iatrogentic displacement and trans- port of benign epithelial cells in patients with bresat carcinoma 显示文摘 | Bleiweiss IJ Nagi CS Jaffer S | 2006 | J Clin Oncol2006，24，13： | 1 |
| 9 | Effect of gypsum on proliferation and differentiation of MC3T3-E1 mouse osteoblastic cells显示文摘 | áron Lazáry Bernadett Balla János P. Kósa Krisztián Bácsi Zsolt Nagy István Takács Péter P. Varga Gábor Speer Péter Lakatos | 2006 | Biomaterials2006，，3： | 1 |
| 10 | Avoidance of vascular compression in decompressive surgery for brain edema caused by trauma and tumor ablation显示文摘 | Csókay A Nagy L Novoth B | 2001 | Neurosurg Rev2001，24，4： | 1 |
| 11 | The mechanism of clustering in supersaturated solid solutions of A1-Mg2Si alloys显示文摘 | KOVáCS I LENDVAI J NAGY E | 1972 | Acta Metallurgica1972，20，7： | 1 |
| 12 | Integrated enzymatic production of specific structured lipid and phytosterol ester compositions显示文摘 | Gabriella Hellner Enik? Rita T?ke Viviána Nagy Gy?rgy Szakács László Poppe | 2010 | Process Biochemistry2010，，8： | 1 |
| 13 | A novel method for investigation of uptake and distribution of polluting microelements and nanoparticles in soil-inhabiting nematodes显示文摘 | Zoltán Sávoly Péter Nagy Gábor Varga Károly Havancsák Krisztina Hrács Gyula Záray | 2013 | Microchemical Journal2013，，： | 1 |
| 14 | Axillary sentinel lymph nodes can be falsely positive due to iatrogenic displacement and transport of benign epithelial cells in patients with breast carcinoma显示文摘 | BLEIWEISS IJ NAGI CS JAFFER S | 2006 | J Clin Oncol2006，24，13： | 1 |
| 15 | Axillary sentinel lymph nodes can be falsely positive due to iatrogenic displace- ment and transport of benign epithelial cells in patients with breast carcinoma 显示文摘 | Bleiweiss 1J Nagi CS Jaffer S | 2006 | J Clin 0ncol2006，24，13： | 1 |
| 16 | Axillary sentinel lymph nodes can be falsely positive due to iatrogenic displacement and transport of benign epithelial cells in patients with breast carcinoma显示文摘 | Bleiweiss IJ Nagi CS Jaffer S | 2006 | J Clin Oncol2006，24，13： | 1 |
| 17 | Role of (myo)fibroblasts in the development of vascular and connective tissue structure of the C38 colorectal cancer in mice显示文摘Background:It remains unclear if the vascular and connective tissue structures of primary and metastatic tumors are intrinsically determined or whether these characteristics are defined by the host tissue.Therefore we examined the microanatomical steps of vasculature and connective tissue development of C38 colon carcinoma in different tissues.Methods:Tumors produced in mice at five different locations(the cecal wall,skin,liver,lung,and brain)were ana-lyzed using fluorescent immunohistochemistry,electron microscopy and quantitative real-time polymerase chain reaction.Results:We found that in the cecal wall,skin,liver,and lung,resident fibroblasts differentiate into collagenous matrix-producing myofibroblasts at the tumor periphery.These activated fibroblasts together with the produced matrix were incorporated by the tumor.The connective tissue development culminated in the appearance of intratumoral tissue columns(centrally located single microvessels embedded in connective tissue and smooth muscle actin-expressing myofibroblasts surrounded by basement membrane).Conversely,in the brain(which lacks fibroblasts),C38 metasta-ses only induced the development of vascularized desmoplastic tissue columns when the growing tumor reached the fibroblast-containing meninges.Conclusions:Our data suggest that the desmoplastic host tissue response is induced by tumor-derived fibrogenic molecules acting on host tissue fibroblasts.We concluded that not only the host tissue characteristics but also the tumor-derived fibrogenic signals determine the vascular and connective tissue structure of tumors. | Edina Bugyik Vanessza Szabó Katalin Dezső András Rókusz Armanda Szücs Péter Nagy József Tóvári Viktória László Balázs Döme Sándor Paku | 2018 | Cancer Communications2018，38，1： | 1 |
| 18 | Axillary sentinel lymph nodes can be falsely positive due to iatrogenie displacement and transport of benign epithelial cells in patients with breast carcinoma显示文摘 | Bleiweiss IJ Nagi CS Jaffer S | 2006 | J Clin Oneol2006，24，13： | 1 |
| 19 | Glutamatergic and GABAergic innervation of human gonadotropin-releasing hor- mone--I neurons显示文摘 | HRABOVSZKY E MOLNAR CS NAGY R | 2012 | Endocrinology2012，153，6： | 1 |
| 20 | Acute phase proteins and variables of protein metabolism in dairy cows during the pre- and postpartal period 显示文摘 | Tothova CS Nagy O Seidel H | 2007 | Acta Vet Brno2007，6，13： | 1 |
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