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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Whipple’s pancreaticoduodenectomy at a resource-poor,low-volume center in Trinidad and Tobago显示文摘BACKGROUND Many authorities advocate for Whipple’s procedures to be performed in highvolume centers,but many patients in poor developing nations cannot access these centers.We sought to determine whether clinical outcomes were acceptable when Whipple’s procedures were performed in a low-volume,resource-poor setting in the West Indies.AIM To study outcomes of Whipple’s procedures in a pancreatic unit in the West Indies over an eight-year period from June 1,2013 to June 30,2021.METHODS This was a retrospective study of all patients undergoing Whipple’s procedures in a pancreatic unit in the West Indies over an eight-year period from June 1,2013 to June 30,2021.RESULTS This center performed an average of 11.25 procedures per annum.There were 72 patients in the final study population at a mean age of 60.2 years,with 52.7%having American Society of Anesthesiologists scores≥III and 54.1%with Eastern Cooperative Oncology Group scores≥2.Open Whipple’s procedures were performed in 70 patients and laparoscopic assisted procedures in 2.Portal vein resection/reconstruction was performed in 19(26.4%)patients.In patients undergoing open procedures there was 367±54.1 min mean operating time,1394±656.8 mL mean blood loss,5.24±7.22 d mean intensive care unit stay and 15.1±9.53 d hospitalization.Six(8.3%)patients experienced minor morbidity,10(14%)major morbidity and there were 4(5.5%)deaths.CONCLUSION This paper adds to the growing body of evidence that volume alone should not be used as a marker of quality for patients requiring Whipple’s procedures.Low volume centers in resource poor nations can achieve good short-term outcomes.This is largely due to the process of continuous,adaptive learning by the entire hospital. | Shamir O Cawich Dexter A Thomas Neil W Pearce Vijay Naraynsingh | 2022 | World Journal of Clinical Oncology2022，13，9： | 2 |
| 3 | Receptor-mediated gene delivery to human peripheral blood mononuclear cells using anti-CD3 antibody coupled to polyethylenimine显示文摘 | O'NEIL M M KENNEDY C A BARTON R W | 2001 | Gene Therapy2001，，8： | 1 |
| 4 | Cytokine therapy:a natural alternative for disease control显示文摘 | Lowenthal J W O Neil T E | 1999 | Vet Immunol Immunopathol1999，，72： | 1 |
| 5 | Experimental p-y model for submerged,stiff clay显示文摘 | Dunnavant T W O'Neil M W | 1989 | Journal of the Geotechnical and Engineering1989，115，1： | 1 |
| 6 | How calmodulin binds its targets: sequence independent recognition of amphiphilic a-helices 显示文摘 | O' Neil K T DeGrado W F | 1990 | Trends Biochem Sci1990，15，2： | 1 |
| 7 | Recombinant chicken IFN-γ expressed in Escherichia coli: Analysis of C-terminal truneation and effeet on biologie aetivity显示文摘 | Miehalski W P Shiell B J O'Neil T E | 1999 | Journal of Interferon & Cytokine Researeh1999，19，4： | 1 |
| 8 | Potential use of cytokine therapy in poultry显示文摘 | Lowenthal J W York J J O'Neil T E | 1998 | Vet Immunol Immunopathol1998，63，12： | 1 |
| 9 | Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences显示文摘 | Zhou W Rousset F O'Neil S | 1998 | Proc Biol Sci1998，265，1395： | 1 |
| 10 | Subband coding of images 显示文摘 | | 1986 | IEEE TRASACTIONS ON ACOUSTICS SPPECH AND SIGNAL PROCESSING1986，34，5： | 1 |
| 11 | Formation age and metamorphic history of the Nuvvuagittuq Greenstone Belt 显示文摘 | O'Neil J Carlson R W Paquette J L | 2012 | Precamhrlan Research2012，22，： | 1 |
| 12 | High-Level Production of Recombinant Chicken Interferon-γ by Brevibacillus choshinensis 显示文摘 | Yashiro K Lowenthal J W O'Neil T E | 2001 | Protein Expression and Purification2001，23，1： | 1 |
| 13 | Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences 显示文摘 | Zhou W Rousset F O'Neil S | 1998 | Proc R Soc Lond B1998，265，1395： | 1 |
| 14 | The effects of power rippling on CO2 laser cutting 显示文摘 | CHENG H NEIL W O | 1997 | Opt Technol1997，29，3： | 1 |
| 15 | Subband coding of images显示文摘 | J W Woods S D O′Neil | 1986 | IEEE Trans Acoust Speech Signal Process1986，34，5： | 1 |
| 16 | Recombinant chicken IFN-gamma expressed in Escherichia coli:Analysis of C-terminal truncation and effect on biologic activity显示文摘 | Michalski W P Shiell B J O Neil T E | 1999 | Interferon Cytokine Res1999，19，： | 1 |
| 17 | Virus-Like Particles Associated with Lyngbya majuscula (Cyanophyta;Oscillatoriacea) Bloom Decline in Moreton Bay,Australia显示文摘 | Hewson I O'Neil J M Dennison W C | 2001 | Aquatic Microbial Ecology2001，25，： | 1 |
| 18 | Neodymium-142 Evidence for Hadean Marie Crust显示文摘 | O'Neil J Carlson R W Francis D Stevenson R K | 2008 | Seience2008，321，： | 1 |
| 19 | Transferable dis charge permits and economic efficiency: The Fox River显示文摘 | O'NEIL W DAVID M MOORE C | 1983 | Journal of Environmental Economics and Management1983，10，4： | 1 |
| 20 | Reliability and validity of a computer--based knowledge mapping system to measure content understanding 显示文摘 | HERL H E O'NEIL JR H F CHUNG G K W K | 1999 | Computer in Human Behavior1999，15，： | 1 |
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