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| 1 | VEGF-D expression correlates with colorectal cancer aggressiveness and is downregulated by cetuximab显示文摘AIM:To gain mechanistic insights into the role played by epidermal growth factor receptor (EGFR) in the regulation of vascular endothelial growth factors (VEGFs) in colorectal cancer (CRC). METHODS:The impact of high-level expression of the growth factor receptors EGFR and VEGF receptor (VEGFR)3 and the VEGFR3 ligands VEGF-C and VEGF-D on disease progression and prognosis in human CRC was investigated in 108 patients using immunohistochemistry. Furthermore, the expression of the lymphangiogenic factors in response to the modulation of EGFR signalling by the EGFR-targeted monoclonal antibody cetuximab was investigated at the mRNA and protein level in human SW480 and SW620 CRC cell lines and a mouse xenograft model. RESULTS: Human CRC specimens and cell lines displayed EGFR, VEGF-C and VEGF-D expression with varying intensities. VEGF-C expression was associated with histological grade. Strong expression of VEGF-D was significantly associated with lymph node metastases and linked to a trend for decreased survival in lymph node-positive patients. EGFR blockade with cetuximab resulted in a significant decrease of VEGF-D expression in vitro and in vivo. CONCLUSION:In conclusion, the expression of VEGF-D in colorectal tumours is significantly associated with lymphatic involvement in CRC patients and such expression might be blocked effectively by cetuximab. | Markus Moehler Christian Frings Annett Mueller Ines Gockel Carl C Schimanski Stefan Biesterfeld Institute of Pathology Johannes Gutenberg University Mainz 55101 Germany Peter R Galle Martin H Holtmann | 2008 | World Journal of Gastroenterology2008，14，26： | 15 |
| 2 | An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita显示文摘Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2015 | Horticulture Research2015，2，1： | 11 |
| 3 | Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin显示文摘Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2016 | Horticulture Research2016，3，1： | 7 |
| 4 | Transient micro-elastography:A novel non-invasive approach to measure liver stiffness in mice显示文摘AIM:To develop and validate a transient micro-elastography device to measure liver stiffness(LS) in mice.METHODS:A novel transient micro-elastography(TME) device,dedicated to LS measurements in mice with a range of measurement from 1-170 kPa,was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston.The novel probe was validated in a classical fibrosis model(CCl4) and in a transgenic murine model of systemic amyloidosis.RESULTS:TME could be successfully performed in control mice below the xiphoid cartilage,with a mean LS of 4.4 ± 1.3 kPa,a mean success rate of 88%,and an excellent intra-observer agreement(0.98).Treatment with CCl4 over seven weeks drastically increased LS as compared to controls(18.2 ± 3.7 kPa vs 3.6 ± 1.2 kPa).Moreover,fibrosis stage was highly correlated with LS(Spearman coefficient = 0.88,P < 0.01).In the amyloidosis model,much higher LS values were obtained,reaching maximum values of > 150 kPa.LS significantly correlated with the amyloidosis index(0.93,P < 0.0001) and the plasma concentration of mutant hapoA-□(0.62,P < 0.005).CONCLUSION:Here,we have established the first non-invasive approach to measure LS in mice,and have successfully validated it in two murine models of high LS. | Cécile Bastard Matteo R Bosisio Michèle Chabert Athina D Kalopissis Meriem Mahrouf-Yorgov Hélène Gilgenkrantz Sebastian Mueller Laurent Sandrin | 2011 | World Journal of Gastroenterology2011，17，8： | 4 |
| 5 | Magnetic and crystallographic properties of several C1b type Heusler compounds 显示文摘 | Helmholdt R B de Groot R A Mueller F M | 1984 | J Magn Magn Mater1984，43，： | 2 |
| 6 | Fermi-liquid-to-polaron crossover Ⅱ double exchange and the physics of colossal magnetoresistance 显示文摘 | Millis A J Mueller R Shraiman B I | 1996 | Phys Rev B1996，54，： | 2 |
| 7 | Olfactory structures in staged human embryos显示文摘 | Mueller F O'Rahilly R | 2004 | Cells Tissues Organs2004，178，2： | 1 |
| 8 | Voltage sags in industrial systems显示文摘 | MCGRANAGHAN M F MUELLER D R SAMOTYJ M J | 1993 | IEEE Trans Industry Applications1993，29，2： | 1 |
| 9 | The CM-SAF Oper-ational Scheme for the Satellite based Retrieval of Solar SurfaceIrradiance-A LUT based Eigenvector Hybrid Approach显示文摘 | Mueller R W Matsoukas C Gratzki A | 2009 | Re-mote Sensing of Environment2009，113，5： | 1 |
| 10 | Impact of cytokeratin-20 and carcinoembryonic antigen mRNA detection by RT-PCR in regional lymph nodes of patients with colorectal cancer显示文摘 | Rosenberg R Hoos A Mueller J | 2000 | Br J Cancer2000，83，： | 1 |
| 11 | Thymosin-α1 stimulates maturation of CD34+ stem cells into CD3+4+ cells in an in vitro thymic epithelia organ coculture model显示文摘 | KNUTSEN A P FREEMAN J J MUELLER K R | 1999 | International Journal of Immunopharmacology1999，21，1： | 1 |
| 12 | Evaluation of oxide dispersionstrengthened ( ODS ) molybdenum and molybdenum - rhenium alloys显示文摘 | Mueller A J Bianco R Buckman R W | 2000 | International Journal of Refractory Metals and Hard Materials2000，18，20： | 1 |
| 13 | Anti-angiogenic treatment of endometriosis : biochemical aspects 显示文摘 | Taylor R N Mueller M D | 2004 | Gynecol Obstet Invest2004，57，1： | 1 |
| 14 | Apple cuhivar re- sponse to 1- methylcyclopropene 显示文摘 | Mueller R S Murr D P DeEu J R | 2001 | Hortscience2001，36，3： | 1 |
| 15 | Sensory gating in schizophrenia:P50 and N100 gating in antipsychotic-free subjects at risk,first-episode,and chronic patients显示文摘 | Brockhaus-Dumke A Schultze-Lutter F Mueller R Tendolkar I Bechdolf A Pukrop R Klosterkoetter J Ruhrmann S | | 0，，5： | 1 |
| 16 | Biological degradation of synthetic polyesters-enzymes as potential catalysts for polyester recycling显示文摘 | MUELLER R | 2006 | Process Biochemistry2006，41，10： | 1 |
| 17 | Fibre Production and SheepBreeding in South America, Proceedings of the 18th Conference Barossa Valley, South Australia 显示文摘 | Cardellino R C Mueller J P | 2009 | Proc Assoc Advmt Anim Breed Genet2009，18，： | 1 |
| 18 | 显示文摘 | Millis A J Boris I Shraiman Mueller R | 1996 | Phys Rev Lett1996，77，： | 1 |
| 19 | Consumer perceptions in East- em European food markets 显示文摘 | Baron P J Mueller R D | 1995 | British Food Journal1995，，2： | 1 |
| 20 | Embryonic critical windows: changes in incubation temperature alter survival, hatchling phenotype, and cost of development in lake whitefish (Coregonus clupeaformis) 显示文摘 | Mueller C A Eme J Manzon R G | 2015 | J Comp Physiol B2015，185，： | 1 |
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