|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC. | Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel | 2017 | World Journal of Stem Cells2017，9，9： | 4 |
| 2 | Crystal-field analysis of triply ionized rare earth ions in lanthanum trifluoride显示文摘 | MORRISON C A LEAVITT R P | 1979 | J Chem Phys1979，71，6： | 2 |
| 3 | An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneurnoniae 显示文摘 | Havarstein L S Coomaraswamy G Morrison D A | 1995 | Proc Natl Acad Sci1995，92，24： | 1 |
| 4 | Effect of a vitamin D analog on leg bone mineral density in patients with chronic spinal cord injury 显示文摘 | Bauman W A Spungen A M Morrison N | 2005 | J Rehabil Res Dev2005，42，5： | 1 |
| 5 | Droplet dynamics in creeping flows显示文摘 | Stewart M B Morrison F A | 1981 | Journal of Applied Mechanics-Transactions of the ASME1981，48，： | 1 |
| 6 | Prediction of bone mineral density from vitamin D receptor alleles显示文摘 | Tokita A | 1994 | Nature1994，367，： | 1 |
| 7 | 显示文摘 | Yee A Morrison S J Idriss H | 2000 | Catal Today2000，63，： | 1 |
| 8 | Utility of a rapid Brain natriuretic peptide assay in differentiating congestive heart failure from lung disease in patients presenting with dyspnea 显示文摘 | Morrison LK Harrison A Krishnaswamy P | 2002 | J Am Coll Cardiol2002，39，： | 1 |
| 9 | A single amino acid change in the Newcastle disease virus fusion protein alters the requirement for HN fusion protein in fusion 显示文摘 | Sergel T A Mcginnes L W Morrison T G | 2000 | Journal of Virology2000，74，11： | 1 |
| 10 | Thumb reconsturction using a free neurovascular wrap-around flap from the big toe显示文摘 | Morrison W A | 1980 | J'' Hand Surg (Br)1980，5，： | 1 |
| 11 | Perceptions of communication, cohesion, and adaptability infamilies of adolescents with andwithout learning handicaps显示文摘 | Morrison G M Zetlin A | 1988 | Journal of Abnormal ClildPsychology1988，16，6： | 1 |
| 12 | Induced side forces at high angles of attack显示文摘 | WARDLAW A B Jr and MORRISON A M | 1976 | Journal of Spacecraft1976，13，10： | 1 |
| 13 | Social Networking显示文摘 | Weaver A C Morrison B B | 2008 | Computer2008，41，2： | 1 |
| 14 | Russia's destination image among American pleasure travelers:Revisiting Echtner and Ritchie 显示文摘 | Stepchenkova S and Morrison A M | 2008 | Tourism Management2008，29，3： | 1 |
| 15 | Entrepreneurship and sustainable tourism:The houseboats of Kerala显示文摘 | Kokkranikal J Morrison A | 2002 | Tourism and Hospitality Research2002，，4： | 1 |
| 16 | Effect of intrathecal magnesium in the presence or absence of local anaesthetic with and without lipophilic opioids : a systematic review and meta-a- nalysis显示文摘 | Morrison A Hunter J Halpern S | 2013 | British Journal of Anaesthesia2013，3，12： | 1 |
| 17 | The Prediction andMeasurement of incompressible flow in a labyrinth seal 显示文摘 | DEMKO J A MORRISON G L RHODE D L | 1980 | ASME Journal of Engineering Gas Turbines Power1980，111，4： | 1 |
| 18 | Identification of a New Regulator in Streptococcus pneumoniac Linking Quorum Sensing to Competence for Genetic Transformation显示文摘 | Lee M S Morrison D A | 1999 | Journal of Bacteriology1999，181，16： | 1 |
| 19 | Freezing of buffy coat-derived,leukoreduced platelet concentrates in 6 percent dimethyl sulfoxide显示文摘 | Hornsey VS McMillan L Morrison A | 2008 | Blood Transfusion2008，48，12： | 1 |
| 20 | A study of ethanol reactions over Pt/CeO2 by temperature-trogrammed desorption and in situ FT-IR spectroscopy:Evidence of benzene formation显示文摘 | YEE A MORRISON S J IDRISS H | 2000 | Journal of Catalysis2000，191，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员