| 1 | Cryopreservation of cynomolgus monkey (Macacafascicularis)spermatozoa in a chemically defined extender显示文摘Aim: To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender.Methods'' Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys.The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris (mTTE) synthetic extender and glycerol as cryoprotectant. The effects of glycerol concentration (1%, 3%, 5%, 10% and 15% [v/v]) and its equilibration time (10min, 30min, 60min and 90min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity. Results: The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P<0.05) for 5% glycerol (42.95±2.55 and 50.39±2.42, respectively) than those of the other groups (1%: 19.19±3.22 and 24.84±3.64; 3%: 34.23±3.43 and 41.37±3.42; 10%:15.68±2.36 and 21.39±3.14; 15%: 7.47±1.44 and 12.90±2.18). The parameters for 30min equilibration (42.95±2.55 and 50.39±2.42) were better (P<0.05) than those of the other groups (10min: 31.33±3.06 and 38.98±3.31; 60min: 32.49±3.86 and 40.01±4.18; 90min: 31.16±3.66 and 38.30±3.78). Five percent glycerol and 30min equilibration yielded the highest post-thaw sperm motility and head membrane integrity. Conclusion:Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender, which is related to the concentration and the equilibration time of glycerol. | Ya-HuiLi Ke-JunCai LeiSu MoGuan Xie-ChaoHe HongWang AndrasKovacs Wei-ZhiJi | 2005 | Asian Journal of Andrology2005，7，2： | 11 |
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