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| 1 | A novel peptide, selected from phage display library of random peptides, can efficiently target into human breast cancer cell显示文摘To develop a targeting vector for breast cancer biotherapy, MDA-MB-231 cell, a human breast cancer cell line, was co-cultured with pC89 (9 aa) phage display library of random peptides. In multiple inde- pendent peptide-presenting phage screening trials, subtilisin was used as a protease to inactivate ex- tra-cellular phages. The internalized phages were collected by cell lysising and amplified in E. coli XLI-Blue. Through five rounds of selection, the peptide-presenting phages which could be internalized in MDA-MB-231 cells were isolated. A comparison was made between internalization capacities of pep- tide-presenting phages isolated from MDA-MB-231 cells and RGD-integrin binding phage by cocultur- ing them with other human tumor cell lines and normal cells. The nucleotide sequences of isolated peptide-presenting phages were then determined by DNA sequencing. To uncover whether phage coat protein or amino acid order was required for the character of the peptide to MDA-MB-231 cells, three peptides were synthesized. They are CASPSGALRSC, ASPSGALRS and CGVIFDHSVPC (the shifted sequence of CASPSGALRSC), and after coculturing them with different cell lines, their targeting ca- pacities to MDA-MB-231 cells were detected. These data suggested that the internalization process was highly selective, and capable of capturing a specific peptide from parent peptide variants. Moreover, the targeting internalization event of peptides was an amino acid sequence dependent manner. The results demonstrated the feasibility of using phage display library of random peptides to develop new targeting system for intracellular delivery of macromolecules, and the peptide we obtained might be modified as a targeting vector for breast cancer gene therapy. | DONG Jian LIU WeiQing ZHANG KeJian JIANG AiMei CHEN MingQing | 2008 | Chinese Science Bulletin2008，53，6： | 12 |
| 2 | Hydrogen peroxide modulates abscisic acid signaling in root growth and development in Arabidopsis显示文摘Exogenous abscisic acid(ABA)can inhibit root growth and promote formation of more root hairs in the root tip of Arabidopsis.However,the molecular mechanisms that underlie root ABA signaling are largely unknown.We report here that hydrogen peroxide(H2O2)reduces the root growth of wild type, and the phenotype of H2O2 on the root growth is similar to ABA response.Meanwhile ABA-induced changes in the morphology of root system can be partly reversed by ascorbic acid in wild type and abolished in NADPH oxidase defective mutant atrbohF and atrbohC.Further,ABA can induce H2O2 accumulation in the root cells and enhance transcription level of OXI1,which is necessary for many more AOS-dependent processes such as root hair growth in Arabidopsis.Our results suggest that H2O2 as an important signal molecule is required for the ABA-regulated root growth and development in Arabidopsis. | BAI Ling ZHOU Yun ZHANG XiaoRan SONG ChunPeng Cao MingQing | 2007 | Chinese Science Bulletin2007，52，8： | 12 |
| 3 | Distribution of plant species and species-soil relationship in the east central Gurbantunggut Desert, China显示文摘在在东方的种类和土壤因素之间的植物种类和关系的分发 Gurbantunggut 沙漠的中央部分被学习提供更多的卓见进植物群并且越过沙漠的各种各样的部分在植被决定差别。双向簇分析证明在区域的植被能被划分成三个组,第一个组被灌木种类, Ephedra przewalskii 和草种类统治,主要在扁平的根据和轻轻的斜坡的区域的 Carex physodes;第二个组被 C 统治。physodes, Artemisia songorica 和 A。主要沙沙丘和第三个组的斜坡上的 xerophytica 被灌木种类统治,主要沙沙丘的顶上的 Haloxylon persicum。在植物密度在之间没有差别组织 1 和 2,但是在组 3 有重要减少。不到植被组 3 的土壤水比在其它,二个组根本玷污深度的低得多。EC,有机物,全部的 P 和可溶的 Na, Ca 和 Mg 与土壤水很同样变化了。正规通讯分析(CCA ) 令人满意地在区域估计了种类土壤关系。植物种类的分发强烈与水内容,有机物, EC 和营养素的土壤因素被相关。在三把 CCA 斧子解释的种类出现的变化是大约 70% ,显示一些解释地点变量可以在我们的学习参数外面存在。土壤质地被建议在未来研究被包括改进 CCA 的解释。 | AN Ping LI Xiangjun ZHENG Yuanrun ENEJI A. Egrinya QIMAN Yunus ZHENG Mingqing INANAGA Shinobu | 2015 | Journal of Geographical Sciences2015，25，1： | 11 |
| 4 | Three independent parameters to describe conventional triaxial compressive strength of intact rocks显示文摘The strengths of 12 rocks cited from literatures increase in a nonlinear way with increasing confining pressure against the Coulomb criterion. The criteria with power forms like the generalized Hoek-Brown criterion are not available for describing the strength properties in the whole test range for Indiana limestone, Yamaguchi marble and Vosges sandstone, of which the differential stresses are approximately constant at high confining pressures. The exponential criterion with three parameters fits the test data of those 12 rocks well with a low misfit. The three parameters are independent of the uniaxial compressive strength (UCS), the initial increasing rate of strength with confining pressure, and the limitation of differential stress. | Mingqing You | 2010 | Journal of Rock Mechanics and Geotechnical Engineering2010，2，4： | 11 |
| 5 | A combination extract of Renshen(Panax Ginseng),Yinyanghuo(Herba Epimedii Brevicornus),Yuanzhi(Radix Palygalae) and Jianghuang(Rhizoma Curcumae Longae) decreases glycogen synthase kinase 3β expression in brain cortex of APPV717I transgenic mice显示文摘OBJECTIVE:To investigate the neuroprotective mechanism of combination extract of Renshen(Panax Ginseng),Yinyanghuo(Herba Epimedii Brevicornus),Yuanzhi(Radix Palygalae) and Jianghuang(Rhizoma Curcumae Longae)(GEPT) in treating Alzheimer's disease on the target of glycogen synthase kinase 3β(GSK-3β).METHODS:Three-month-old APPV717I transgenic mice were randomly divided into ten groups(n=12 per group) and intragastrically administrated vehicle or medicines:APP group was given 0.5% CMC,donepezil group was given donepezil(APP + D group)(0.92 mg/kg-1·day-1),and GEPT groups were given small dose of GEPT(APP+Gs group)(0.075 g/kg-1·day-1),medium dose(APP+Gm group)(0.15 g/kg-1·day-1),and large dose(APP+Gl group)(0.30 g/kg-1·day-1) for 4 or 8 months,respectively.Three-month-old C57BL/6J mice as vehicle controls(n=12) were given 0.5% CMC for 4 or 8 months as well.The GSK-3β expression in the cortex of 7-and 11-month-old APPV717I transgenic mice with and without GEPT or donepezil treatment and normal C57BL/6J mice were measured via Western blotting and Immunohistochemistry.RESULTS:Immunohistochemistry analysis showed significant increase of GSK-3β in the cerebral cortex of 7-month-old APP group(compare to control group P=0.003),while the GSK-3β expression of donepezil or GEPT group were all significantly decreased(Donepezil vs APP:P=0.041;Gl vs APP:P= 0.049;Gm vs APP:P=0.029;Gh vs APP:P=0.036).Western blot analysis showed similar results.The densitometric measures of GSK-3β in APP mice increased significantly as compared with the control group(P=0.008).And the GSK-3β expression in donepezil and GEPT groups were all decreased.There was significant difference between Gh group or donepezil group and the control group(P=0.05).Similar findings were shown in the 11-month-old mice in each group,except for greater decrease of GSK-3β in the GEPT group.CONCLUSION:GEPT can effectively decrease the level of GSK-3β expression in the brain cortex of APPV717I transgenic mice,and such effect is more significant in 11-month-old mice.This partially explains the neuroprotecting mechanism of GEPT in preventing and treating of AD. | Jing Shi Jinzhou Tian Xuekai Zhang Chuiyou Zeng Mingqing Wei Pengwen Wang Yongyan Wang | 2013 | Journal of Traditional Chinese Medicine2013，33，2： | 6 |
| 6 | Effect of rice-rice-rape rotation on physicochemical property and bacterial community of rhizosphere soil显示文摘Through collecting rhizosphere soil sample from a 30-year long-term fixed location test site that use“rice-ricerape”crop rotation(RRR)and“rice-rice-fallow”continuous cropping systems(RRF),this paper investigated effects of long-term crop rotation on physicochemical property and bacterial community of rhizosphere soil.Results showed that total nitrogen(TN),total phosphorus(TP)and available potassium(AK)contents in rhizosphere soil under long-term RRR were decreased by 28.09%,15.69%and 6.25%respectively.Alkali-hydrolyzable nitrogen(AN)and available phosphorus(AP)contents were 10.59%and 13.25%higher than those of soil in RRF respectively.Three soil samples collected during different periods also showed that RRR resulted in a lower rhizosphere soil pH than RRF.Clone library analysis revealed that significant difference in rhizosphere soil bacterial community was observed between RRR and RRF continuous cropping.Abundance ofα-Proteobacteria,β-Proteobacteria andγ-Proteobacteria were higher in rhizosphere soil of RRR compared to RRF.pH of rhizosphere soil was significantly correlated with Acidobacteria level,while total organic carbon(TOC)content was significantly correlated with Proteobacteria level.Long-term RRR enhanced conversion of N and P in rhizosphere soil,increased bio-availability to crop,and promoted diversity of soil bacterial community.Bacterial diversity in RRR could be ecological significance in maintaining soil fertility and functionality. | Licheng Zhang Juan Li Mingqing Zhang | 2020 | Oil Crop Science2020，5，3： | 4 |
| 7 | Strength and damage of marble in ductile failure显示文摘To study the effects of loading paths and stress states on rock strength and deformation, marble specimens were axially compressed to various displacements under a confining pressure (CP) firstly, and then the damaged specimens were recompressed under another CP. The bearing capacity of a marble specimen depends merely on CP at the stage of ductile deformation, and it has no relation with the loading history when CP keeps constant or increases. However, the damaged specimen turns into brittle when it is recompressed uniaxially or at a lower CP, and the Young's modulus and strength are lower than those of a dense specimen. The increasing ratio of triaxial strength to CP has a close relation with the areas of fissures in the damaged specimens but not the internal friction angle. Material strength and bearing capacity are two different conceptions for rocks. Material strength decreases continually as the plastic deformation increases; however, the bearing capacity is determined by both the stress state and the material strength. | Mingqing You School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, 454010, China | 2011 | Journal of Rock Mechanics and Geotechnical Engineering2011，3，2： | 4 |
| 8 | Difluoromethylation of 2-Hydroxychalcones Using Sodium 2-Chloro-2,2-difluoroacetate as Difluoromethylating Agent显示文摘 | WANG Wei HUA Mingqing HUANG Yan ZHANG Qi ZHANG Xiaoyan WU Jingbo ZHANG Qi | 2015 | Chemical Research in Chinese Universities2015，31，3： | 3 |
| 9 | Prevalence and trends of aminoglycoside resistance in Shigella worldwide, 1999-2010显示文摘Shigellosis causes diarrheal disease in humans in both developed and developing countries, and multi-drug resistance in Shigella is an emerging problem. Understanding changing resistance patterns is important in determining appropriate antibiotic treatments. This meta-analysis systematically evaluated aminoglycoside resist-ance in Shigella. A systematic review was constructed based on MEDLINE and EMBASE databases. Randomeffect models or fixed-effect models were used based on P value considering the possibility of heterogeneity between studies for meta-analysis. Data manipulation and statistical analyses were performed using software STATA 11.0. By means of meta-analysis, we found a lower resistance to three kinds of aminoglycosides in the Europe-America areas during the 12 year study period than that of the Asia-Africa areas. Kanamycin resistance was observed to be the most common drug resistance among Shigella isolates with a prevalence of 6.88% (95%CI: 6.36%-7.43%). Comparison of data from Europe-America and Asia-Africa areas revealed that Shigella flexneri resistance was greater than the resistance calculated for Shigella sonnei. Importantly,Shigella sonnei has played a significant role in aminoglycoside-resistance in recent years. Similarly, data showed that resistance to these drugs in children was higher than the corresponding data of adults. In conclusion, aminoglycoside-resistant Shigella is not an unusual phenomenon worldwide. Distribution in Shigella resistance differs sharply based on geographic areas, periods of time and subtypes. The results from the present study highlight the need for continuous surveillance of resistance and control of antibiotic usage. | Bing Gu Xing Ke Shiyang Pan Yan Cao Ling Zhuang Rongbin Yu Huimin Qian Genyan Liu Mingqing Tong | 2013 | The Journal of Biomedical Research2013，27，2： | 3 |
| 10 | Abnormal regulation of chemokine TECK and its receptor CCR9 in the endometriotic milieu is involved in pathogenesis of endometriosis by way of enhancing invasiveness of endometrial stromal cells显示文摘The chemokine thymus-expressed chemokine(TECK),which regulates T-cell development and tissue-specific homing,has been identified as a potential contributor to the pathogenesis and progression of endometriosis.Dioxin(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD),an air pollutant,and estrogen also appear to be involved in endometriosis.Both endometrial stromal cells(ESCs)and the combination of 17b-estradiol and TCDD increase the secretion of TECK in the endometriosis-associated cells and promote the invasiveness of ESCs by increasing expression of matrix metalloproteinase(MMP)-2 and MMP-9.Anti-TECK neutralizing antibodies can effectively inhibit the invasiveness of ESCs and the expression of MMP-2 and MMP-9 in the cells.Interestingly,the expression of chemokine C receptor 9(CCR9)and its ligand TECK increases significantly in the endometriotic milieu of patients with endometriosis.Therefore,the over-expressed TECK interacts with CCR9 on the ESCs in the endometriotic milieu,which may contribute to the onset and progression of endometriosis. | Yun Wang Jing Yu Xuezhen Luo Xiaoqiu Wang Mingqing Li Ling Wang Dajin Li | 2010 | Cellular & Molecular Immunology2010，7，1： | 3 |
| 11 | 胎儿期恰逢1959~1961年饥荒的中国成人精神分裂症的发病率显示文摘背景:精神分裂症是一种常见的中度精神障碍。子宫内营养缺乏可增大患精神分裂症的危险。其主要依据来自1944~1945年荷兰饥饿冬天(Dutch Hunger Winter)研究,当时食品摄入在短期内极剧下降。这项有关饥荒期间受孕人群的大型队列研究显示,发生精神分裂症的危险增大两倍。
目的:确定经受1959~1961年大饥荒的中国人是否有相似的结果。
设计、地点及参试者:发生精神分裂症危险的调查于安徽省芜湖地区进行,这是当初受灾最重的地区之一。对饥荒之前、饥荒期间和饥荒以后出生的人群发病率进行比较。芜湖及其周围6个县是由一所精神病院负责的。对1971~2001年所有的精神病病历记录均进行检查。精神分裂症患者的临床和社会人口学资料由对自然灾害暴露情况不知情的研究者摘录。有关饥荒年出生人数和死亡人数的数据是有效可用的,累积死亡率根据以后的人口学调查进行估计。
主要观测指标:饥荒的证据已经核实,计算未校正的以及对死亡率进行校正后的发生精神分裂症的相对危险度。
结果:安徽省的出生率(每1000人)在饥荒期问降低了约80%,从1958年的28.28降至1959年的20.97、1960年的8.61和1961年的11.06。在饥荒期间出生的人,晚年发生精神分裂症的校正后危险明显增高,从1959年的0.84%增至1960年的2.15%和1961年的1.81%。死亡率校正相对危险在1960年出生者为2.30(95%可信区间,1.99~2.05),1961年出生者为1.93(95%可信区间,1.68~2.23)。
结论:我们的调查重复了荷兰不同种族人群的数据,结果显示,出生前遭受饥荒可增高晚年患精神分裂症的危险。 | David St Clair Mingqing Xu Peng Wang Yaqin Yu Yourong Fang Feng Zhang Xiaoying Zheng Niufan Gu Guoyin Feng Pak Sham Lin He 张继志(译) | 2006 | 美国医学会杂志(中文版)2006，25，4： | 3 |
| 12 | Synthesis of Visible-Light-Driven g-C3N4/PPy/Ag Ternary Photocatalyst with Improved Photocatalytic Performance显示文摘 | Fengli Zhang Fang Duan Zhigang Ding Mingqing Chen | 2017 | Chinese Journal of Chemistry2017，35，2： | 3 |
| 13 | Closely packed nanoparticle monolayer as a strain gauge fabricated by convective assembly at a confined angle显示文摘 | Chao Zhang Juan Li Shanshan Yang Weihong Jiao Shuang Xiao Mingqing Zou Songliu Yuan Fei Xiao Shuai Wang Lihua Qian | 2014 | Nano Research2014，7，6： | 2 |
| 14 | Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis显示文摘The adenoma-carcinoma sequence is a well-accepted roadmap for the development of sporadic colorectal cancer.However,cellular heterogeneity in aberrant epithelial cells limits our understanding of carcinogenesis in colorectal tissues.Here,we performed a single-cell RNA sequencing survey of 54,788 cells from patient-matched tissue samples,including blood,normal tissue,para-cancer,polyp,and colorectal cancer. | Xiaobo Zheng Jinen Song Chune Yu Zongguang Zhou Xiaowei Liu Jing Yu Guangchao Xu Jiqiao Yang Xiujing He Xin Bai Ya Luo Yu Bao Huifang Li Lie Yang Mingqing Xu Nan Song Xiaodong Su Jie Xu Xuelei Ma Hubing Shi | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 15 | PRACTICE AND DEVELOPMENT ON ZHU'S AND CHAU'S SCALP ACUPUNCTURE显示文摘Zhu’s and Chau’s Scalp Acupuncture is one of the newly developed needling meth-ods which follows the 'penetrating head acu-point needling' method. Through many years ofclinical experience, exploration and research in conjunction with the various advantages of differ-ent styles and sects of scalp acupuncture needling methods, a unique method of scalp acupuncturehas been developed. This method combines the modern medical theory and the traditional Chinesemedical theory. The region of head is divided into seven treatment areas: Shenting region (GV24), Touwei region (ST 8), Shuaigu region (GB 8), Baihui region (GV 2O), Qiangjian region(GV 18), Xinhui region (GV 22), Wangu region (GB 12). The manipulation is developed andcreated according to the Collection of Acupuncture and Moxibustion, Chouqi and Jinqi methods.In the process of the insertion of the needles, this method requires the cooperation of rapid andslow breathing by lifting, following and opposing the meridian course, including active and pas-sive | Zhu Mingqing Zhang Zhibin Jackson ChauChinese Acupuncture Institute of U. S. A. | 1996 | World Journal of Acupuncture-Moxibustion1996，6，3： | 2 |
| 16 | True-triaxial strength criteria for rock显示文摘 | Mingqing You | 2008 | International Journal of Rock Mechanics and Mining Sciences2008，，1： | 2 |
| 17 | A Study on Thermal Self-monitoring of Carbon Fiber Reinforced Concrete显示文摘 | Sun Mingqing Li Zhuoqiu Mao Qizhao | 1999 | Cement and Concrete Research1999，29，： | 1 |
| 18 | A Study on Thermal Self-diagnostic and Self-adaptive Smart Concrete Structures显示文摘 | Sun Mingqing Li Zhuoqiu Liu Qingping | 2000 | Cement and Concrete Research2000，30，12： | 1 |
| 19 | Size effect and loading rate dependence of the pressure-sensitivity of carbon fiber reinforced concrete 显示文摘 | Mingqing Sun | 1998 | Journal of Wuhan University of Technology1998，13，3： | 1 |
| 20 | Fractal characterization of spontaneous co-current imbibition in porous media显示文摘 | CAI Jianchao YU Boming ZOU Mingqing | 2010 | Energy Fuels2010，24，3： | 1 |
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