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| 1 | Aquaporin-1 Expressed in Cultured Human Trabecular Meshwork Cells显示文摘Objective:To determine if aquaporin-1 could be detected in cultures of human trabecularshwork cells. Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study the endogenous expression of aquaporin-1 and its possible role inthe regulation of aqueous outflow. | Mingkai Lin, Jian Ge, Yehong Zhuo, Yuqing Lan, Keming Yu, Jianliang ZhengZhongshan Ophthalmic center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | Eye Science2002，18，1X： | 2 |
| 2 | Phacoemulsification in Patients with Secondary Glaucoma Caused by Uveitis显示文摘Purposes: To evaluate the effect of phacoemulsification in the patients with uveitis,secondary glaucoma and complicated cataract.
Methods: Phacoemulsification and implantation of a foldable intraocular len was performed in 12 patients(13 eyes) with uveitis, secondary glaucoma and complicated cataract. The complications, intraocular pressures (IOP), and visual acuity were observed postoperatively.
Results: No severe complication was found in the patients postoperatively or within the operation procedure. The visual acuity was improved after the operation ( P < 0.05) .The intraocular pressures and/or the number of antiglaucoma medications reduced in 3months of the follow-up time.
Conclusion: phacoemulsification is the best way to treat the patients with secondary glaucoma and complicated cataract caused by uveitis. Eye Science 2001; 17: 8 ~ 10. | Mingkai Lin, Jian Ge, Yizhi Liu, Yuqing Lan, Yehong ZhouZhongshan Ophthalmic Center, Sun Yat-Sen University of Medical Sciences. Guangzhou, 510060, China | 2001 | Eye Science2001，17，1： | 1 |
| 3 | Ten-year Follow-up of Familial Nanophthalmos in Three Siblings显示文摘Purpose: Nanophthalmos is a rare congenital disorder associated with a high incidence of angle-closure glaucoma..We followed the clinical manifestations of three siblings to evaluate their responses to various treatments.Methods:.Three sisters with nanophthalmos were followed from 2000 to 2013..Glaucoma and cataract treatments were performed whenever indicated.Results: The oldest sister had chronic elevation of intraocular pressure(IOP) and underwent laser peripheral iridotomy(LPI)on both eyes,.followed by uneventful phacoemulsification with intraocular lens(IOL) implantation on the left eye and phacotrabeculectomy with IOL implantation on the right eye.The middle sister had acute elevation of IOP and initially underwent phacoemulsification combined with implantation of two IOLs on her left eye and LPI on her right eye. Severe uveal effusion occurred when phacoemulsification was performed on her right eye 6 years later, but ultimately was completely resolved. In both sisters, stable IOP and visual results were achieved after lensectomy. The youngest sister, who had suspected angle-closure, achieved a stable IOP and visual results with prophylactic LPI alone.Conclusion: In nanophthalmic eyes, the severity of the disease may foreshadow the severity of surgical complications and responses to therapy. | Jingjing Huang Yunlan Ling Mingkai Lin Ziqiang Wu Bing Cheng Jian Ge | 2013 | Eye Science2013，28，3： | 1 |
| 4 | The Preliminary Study of Interferon-γ Gene Transfection to Human Tenon's Capsule Fibroblasts in Vitro显示文摘 | Yuqing Lan, Jian Ge, Mingkai Lin, Jianliang Zheng, Huiyi Chen, Haiquan Liu, Jing Wei , Yanyan LiZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China Sun Yat-Sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China | 2000 | 眼科学报2000，16，3： | 1 |
| 5 | High-dimensional zinc porphyrin nanoframeworks as efficient radiosensitizers for cervical cancer显示文摘Radiotherapy is widely used clinically, but the toxic and side effects of nonselective killing of high-energy radiation limit its application. Finding biocompatible materials to assemble radiotherapy sensitizers and studying their sensitization patterns are of great significance for the clinical application. Here, biocompatible zinc porphyrin was chosen as sub-unit to construct various dimensional coordination frameworks. By employing top-down approach, suitable nanoframeworks with various dimensional zinc porphyrin were synthesized as radiosensitizers. The experimental data showed that high-dimensional zinc porphyrin nanoframeworks exhibit higher X-ray response performance. | Fei Cai Kun Ye Mingkai Chen Yuan Tian Peicong Chen Hao Lin Tianfeng Chen Li Ma | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 6 | Diagnostic performance of elastography on liver fibrosis in antiviral treatment-naive chronic hepatitis B patients:a meta-analysis显示文摘Background:This study aimed to assess the performance of transient elastography(TE),two-dimensional shear wave elastography(2D-SWE),and magnetic resonance elastography(MRE)for staging significant fibrosis and cirrhosis in untreated chronic hepatitis B(CHB)patients.Methods:Pubmed,Embase,Web of Science,and Cochrane Library were searched for terms involving CHB,TE,2D-SWE,and MRE.Other etiologies of chronic liver disease,previous treatment in patients,or articles not published in SCI journals were excluded.Hierarchical non-linear models were used to evaluate the diagnostic accuracy of TE,2D-SWE,and MRE.Heterogeneity was explored via analysis of threshold effect and meta-regression.Results:Twenty-eight articles with a total of 4,540 untreated CHB patients were included.The summary areas under the receiver-operating characteristic curves(AUROCs)using TE,2D-SWE,and MRE for predicting significant fibrosis(SF)were 0.84,0.89,and 0.99,respectively.The AUROC values of TE,2D-SWE,and MRE for staging cirrhosis were 0.9,0.94,and 0.99,respectively.Based on the meta-analysis of studies with head-to-head comparison,2D-SWE is superior to TE(0.92 vs 0.85,P<0.01)in staging significant fibrosis.Conclusion:TE,2D-SWE,and MRE express acceptable diagnostic accuracies in staging significant fibrosis and cirrhosis in untreated CHB patients.2D-SWE outperforms TE in detecting significant fibrosis in treatment-naive people with hepatitis B virus. | Li Mingkai Wan Sizhe Wu Xiaoying Lin Ying Bin Wu | 2022 | Gastroenterology Report2022，10，1： | 0 |
| 7 | PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis显示文摘Glaucoma is the leading cause of irreversible blindness worldwide.In the pathogen-esis of glaucoma,activated microglia can lead to retinal ganglion cells(RGCs)apoptosis and death,however,the molecular mechanisms remain largely unknown.We demonstrate that phospholipid scramblase 1(PLSCR1)is a key regulator promoting RGCs apoptosis and their clearance by microglia.As evidenced in retinal progenitor cells and RGCs of the acute ocular hypertension(AOH)mouse model,overexpressed PLSCR1 induced its translocation from the nucleus to the cytoplasm and cytomembrane,as well as elevated phosphatidylserine exposure and reactive oxygen species generation with subsequent RGCs apoptosis and death.These damages were effectively attenuated by PLSCR1 inhibition.In the AOH model,PLSCR1 led to an increase in M1 type microglia activation and retinal neuroinflammation.Upregulation of PLSCR1 resulted in strongly elevated phagocytosis of apoptotic RGCs by activated microglia.Taken together,our study provides important insights linking activated microglia to RGCs death in the glaucoma pathogenesis and other RGC-related neurodegenerative diseases. | Jingyi Luo Qing Lian Deliang Zhu Minglei Zhao Tingfang Mei Bizhi Shang Zeqiu Yang Chujun Liu Wenchang Xu Lan Zhou Keling Wu Xinqi Liu Yuhua Lai Fuxiang Mao Weihua Li Chengguo Zuo Kang Zhang Mingkai Lin Yehong Zhuo Yizhi Liu Lin Lu Ling Zhao | 2023 | Genes & Diseases2023，10，4： | 0 |
| 8 | Marked elevation of serum alpha-fetoprotein following Clonorchis sinensis infection: A rare case report显示文摘Clonorchiasis,also known as the liver fluke disease,is caused by eating raw freshwater fish or shrimps that contain Clonorchis sinensis cyst larvae,which mainly involves the hepatobiliary system.Chronic abdominal pain and distention,loss of appetite,and hepatomegaly are the most common clinical manifestation.Persistent infection with Clonorchis sinensis would result in chronic inflammation,epithelial hyperplasia,and periductal fibrosis and even progress to cholangiocarcinoma.Nevertheless,the majority of the infected people are asymptomatic or mildly symptomatic,contributing to its high misdiagnosis rate.Alpha-fetoprotein(AFP)is a well-known biomarker for hepatocellular carcinoma(HCC).A high level of AFP can also be caused by several benign diseases,causing confusion and influ-encing treatment decisions.So far,clonorchiasis with a markedly elevated level of AFP has been rarely reported.We present a case of clonorchiasis with a high level of AFP in a 52-year-old man.We hope to raise clinical awareness of this food-borne disease.Clonorchiasis should be considered in patients with extremely high levels of AFP,excluding HCC and germ cell tumors,especially if the patient has a history of ingesting raw freshwater fish or shrimps. | Ying Lin Xiaodan Zong Mingkai Li Sizhe Wan Hongsheng Yu Xiuqing Wei | 2022 | Liver Research2022，6，1： | 0 |
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