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| 1 | The Relationship between Glucose Excursion and Cognitive Function in Aged Type 2 Diabetes Patients显示文摘Objective Evidence suggests that type 2 diabetes (T2DM) is associated with an increased risk of dementia and that glucose variability is an independent risk factor for diabetic complications.This study investigated the relationship between glucose excursion and cognitive function in aged T2DM patients.Methods A total of 248 aged T2DM patients wore a continuous glucose monitoring system (CGMS) for 3 days in order to evaluate glucose excursion,including mean amplitude of glycemic excursions (MAGE) and mean of daily difference (MODD).All subjects were evaluated with a number of accepted cognitive function tests,including themini‐mental status examination (MMSE).The relationship between MAGE and MODD and performance on these cognitive tests was assessed.Results The MAGE and MMSE score were negatively correlated,likewise with the correlation between MODD and MMSE.Liner multivariate regression analysis showed that MAGE and MODD were also negatively related to MMSE independent of age,sex,glycemic control,hypertension,smoking,or coronary heart disease history.Conclusion Glucose excursion is related to cognitive function in aged T2DM patients.Elevated glucose excursion decreased the MMSE score,which reflects general cognitive function.Thus,therapy aimed at controlling glucose excursion may be beneficial for maintaining cognitive function in aged T2DM patients. | ZHONG Yuan ZHANG Xiao Yan MIAO Ya ZHU Jie Hua YAN Hong WANG Bei Yun JIN Jun HU Ting Jun JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 34 |
| 2 | Characteristics of one-year observation of VOCs,NOx, and O_3 at an urban site in Wuhan, China显示文摘A continuous online observation of ozone and its precursors(NOx, VOCs) was carried out in central urban Wuhan from September 2016 to August 2017. The concentration levels of ozone,NOx, VOCs and their variations in urban Wuhan were analyzed, as well as effects of VOCs on ozone photochemical generation and the main controlling factors for ozone production. During the observation period, the average concentrations of ozone and NOx in Wuhan was 22.63 and30.14 ppbv, respectively, and the average concentration of VOCs was 32.61 ppbv(42.3% alkanes,13.0% alkenes, 10.0% aromatics, 7.3% acetylene, 9.9% OVOCs, and 10.5% halohydrocarbons).Ozone concentration was higher in spring and summer as compared with autumn and winter,wheras VOCs and NOx concentratios were lower in spring and summer but higher in autumn and winter. Aromatics and alkenes, two of VOCs species, showed the highest contributions to ozone formation potential in Wuhan(35.7% alkenes, 35.4 aromatics, 17.5% alkanes, 8.6% OVOCs,1.6% halogenated hydrocarbons, and 1.4% acetylene). Among all VOCs species, those with the highest contribution were ethylene, m-xylene, toluene, propylene and o-xylene. The contribution of these five compounds to the total ozone formation potential concentration was 43.90%.Ozone-controlling factors in Wuhan changed within one day; during the early morning hours(6:00–9:00), VOCs/NOx was low, and ozone generation followed a VOCs-limited regime.However, during the peak time of ozone concentration(12:00–16:00), the ratio of VOCs/NOx was relatively high, suggesting that ozone generation followed a NOx-limited regime. | Yichang Yang Xingang Liu Jun Zheng Qinwen Tan Miao Feng Yu Qu Junling An Nianliang Cheng | 2019 | Journal of Environmental Sciences2019，31，5： | 26 |
| 3 | Comparison of parathyroid hormone (1-34) and elcatonin in postmenopausal women with osteoporosis: an 18-month randomized, multicenter controlled trial in China显示文摘 | LI Ying XUAN Miao WANG Bo YANG Jun ZHANG Hong ZHANG Xiu-zhen GUO Xiao-hui Lü Xiao-feng XUE Qing-yun YANG Gang-yi JI Qiu-he LIU Zhi-min LI Cheng-jiang WU Tian-feng SHENG Zheng-yan LI Peng-qiu TONG Jiu-cui | 2013 | Chinese Medical Journal2013，，3： | 24 |
| 4 | Paclitaxel ameliorates fibrosis in hepatic stellate cells via inhibition of TGF-β/Smad activity显示文摘AIM: To investigated if paclitaxel can attenuate hepatic fi brosis in rat hepatic stellate cells (RHSCs). METHODS: RHSCs were cultured in vitro and randomly assigned to four groups: normal control group (treated only with Dulbecco's Modified Eagle's Medium), Taxol group (200 nmol/L paclitaxel was added to the cell culture), transforming growth factor (TGF)-β group (5 ng/mL recombinant human TGF-β1 was added to the cell culture), and TGF-β + Taxol group. TGF-β signaling cascade and status of various extracellular matrix proteins were evaluated by real time reverse transcriptase polymerase chain reaction and Western blotting. RESULTS: The paclitaxel treatment markedly suppressed Smad2/3 phosphorylation. This was associated with attenuated expression of collagen Ⅰ and Ⅲ and fi bronectin in RHSCs.CONCLUSION: These data indicate that 200 nmol/L paclitaxel ameliorates hepatic fi brosis via modulating TGF-β signaling, and that paclitaxel may have some therapeutic value in humans with hepatic fi brosis. | Jun Zhou, De-Wu Zhong, Qun-Wei Wang, Xiong-Ying Miao, Xun-Di Xu, Department of Hepatobiliary Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China | 2010 | World Journal of Gastroenterology2010，16，26： | 17 |
| 5 | Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial community structure显示文摘It is unclear whether certain plant species and plant diversity could reduce the impacts of multiple heavy metal pollution on soil microbial structure and soil enzyme activities. Random amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity and microbial similarity in planted and unplanted soil under combined cadmium (Cd) and lead (Pb) pollution. A metal hyper- accumulator, Brassica juncea, and a common plant, Festuca arundinacea Schreb, were used in this research. The results showed that microorganism quantity in planted soil significantly increased, compared with that in unplanted soil with Cd and Pb pollution. The order of microbial community sensitivity in response to Cd and Pb stress was as follows: actinomycetes 〉 bacteria 〉 fungi. Respiration, phosphatase, urease and dehydrogenase activity were significantly inhibited due to Cd and Pb stress. Compared with unplanted soil, planted soils have frequently been reported to have higher rates of microbial activity due to the presence of additional surfaces for microbial colonization and organic compounds released by the plant roots. Two coexisting plants could increase microbe population and the activity of phosphatases, dehydrogenases and, in particular, ureases. Soil enzyme activity was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil in this study. Heavy metal pollution decreased the richness of the soil microbial community, but plant diversity increased DNA sequence diversity and maintained DNA sequence diversity at highlevels. The genetic polymorphism under heavy metal stress was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil. | Yang GAO Chiyuan MIAO Jun XIA Liang MAO Yafeng WANG Pei ZHOU | 2012 | Frontiers of Environmental Science & Engineering2012，6，2： | 14 |
| 6 | Impact of Cardiovascular Disease Deaths on Life Expectancy in Chinese Population显示文摘Objective We aimed to analyze the impact of cardiovascular disease(CVD) deaths on life expectancy(LE) in Chinese population and estimate the percentage reduction in CVD mortality needed to increase LE by 1 year from the current level, a national target of health improvement. Methods We used life tables, cause-elimination life tables, and age decomposition of LE with corrected mortality data from the National Disease Surveillance System in 2010. Results LE at birth of Chinese people was 73.24 years in 2010. Women had a longer LE than men, and urban population had a longer LE than rural population. CVD deaths resulted in a 4.79-year LE loss and premature deaths in people aged 25 to 64 years were responsible for a substantial part of LE loss from CVD. Death from ischemic heart disease and cerebrovascular diseases accounted for 69.2% of LE loss from CVD deaths and death from cerebrovascular diseases was the largest contributor. In rural men, 51.1% LE loss from CVD deaths was caused by cerebrovascular diseases. If there were no changes in mortality rates for all other diseases, a 27.4% reduction in CVD mortality would increase LE by 1 year in Chinese population. Conclusion There is a considerable impact of CVD deaths on LE. A 1-year LE increase in the future requires at least a 27.4% reduction in CVD mortality from the current level. Targeting the rural population and tackling cerebrovascular diseases are important for reaching the national goal of health improvement. | FAN Jie LI Guo Qi LIU Jing WANG Wei WANG Miao QI Yue XIE Wu Xiang LIU Jun ZHAO Fan LI Yan ZHAO Dong | 2014 | Biomedical and Environmental Sciences2014，27，3： | 13 |
| 7 | A randomized, multicenter controlled trial to compare the efficacy of recombinant human parathyroid hormone (1-34) with elcatonin in postmenopausal women with osteoporosis in China显示文摘背景 Recombinant 人的甲状旁腺的荷尔蒙(1-34 )(rhPTH (注射给的 1 -34)) 一新生物产品的第七班药,它被采用基因再结合准备技术。rhPTH (1 -34) 主要被用来对待骨质疏松症,特别为绝经后的 women.This,学习为在中国的 11 个城市的区域与骨质疏松症对待绝经后的女人把 rhPTH (1-34 ) 的临床的功效和安全与 elcatonin 作比较。有骨质疏松症的 205 个女人被注册在的方法一 6 月, multicenter,使随机化的、控制学习。他们被使随机化收到任何一个 rhPTH (1-34 ) 20 ug (200 U ) 日报或 elcatonin 20 U 周刊。腰部的脊骨(L1-4 ) 和大腿骨的颈骨头矿物质密度(BMD ) ,以及骨头周转的生物化学的标记被测量。不利事件是增加的 recorded.Results rhPTH (1-34 ) 腰部的 BMD 非常显著地在 3 个月和 6 个月做了 elcatonin (2.38% 对 0.59% , P < 0.05;5.51% 对 1.55% , P < 0.01 ) ,但是没有在在大腿骨的颈的这二个组的 BMD 的重要增加。在 rhPTH 的骨头标记有更大的吝啬的增加(1 个 -34) 组比在在 3 个月和 6 个月的 elcatonin 组(浆液骨头特定的碱的磷酸酶(BSAP )36.79% 对 0.31% ;92.42% 对 -0.17% ;尿 N-telopeptide/creatinine (NTX/Cr )48.91% 对 -5.32% ;68.82% 对 -10.86%) 。处理很好被容忍,没有重要差别,在任何不利事件和任何严肃的不利事件的比例在二个组之间检测(67.0% 对 59.0% ;0 对 0 ).Conclusions rhPTH (1-34 ) 比 elcatonin 在骨头形成上有更积极的效果,由腰部的 BMD 和骨头形成标记的更大的增长出现与在肝的仅仅温和的不利事件和没有重要变化,肾或 hematological 索引。 | ZHANG Xiu-zhen WANG Bo YANG Jun XUAN Miao SONG Li-ge LI Hong GUO Xiao-hui LU Xiao-feng XUE Qing-yun YANG Gang-yi JI Qiu-he SHEN Jie LIU Zhi-min LI Cheng-jiang WU Tian-feng TONG Xiao-cui JIA Yuan | 2009 | Chinese Medical Journal2009，，24： | 12 |
| 8 | ANALYTICAL MODELS OF FLOATING BRIDGES SUBJECTED BY MOVING LOADS FOR DIFFERENT WATER DEPTHS显示文摘There are two types of floating bridge such as discrete-pontoon floating bridges and continuous-pontoon floating bridges. Analytical models of both floating bridges subjected by moving loads are presented to study the dynamic responses with hydrodynamic influence coefficients for different water depths. The beam theory and potential theory are introduced to produce the models. The hydrodynamic coefficients and dynamic responses of bridges are evaluated by the boundary element method and by the Galerkin method of weighted residuals, respectively. Considering causal relationship between the frequencies of the oscillation of floating bridges and the added mass coefficients, an iteration method is introduced to compute hydrodynamic frequencies. The results indicate that water depth has little influence upon the dynamic responses of both types of floating bridges, so that the effect of water depth can be neglected during the course of designing floating bridges. | ZHANG Jun MIAO Guo-ping LIU Jian-xun SUN Wen-jun | 2008 | Journal of Hydrodynamics2008，20，5： | 12 |
| 9 | m6A RNA modification controls autophagy through upregulating ULK1 protein abundance显示文摘 | Shouheng Jin Xiya Zhang Yanyan Miao Puping Liang Kaiyu Zhu Yuanchu She Yaoxing Wu Di-Ao Liu Junjiu Huang Jian Ren Jun Cui | 2018 | Cell Research2018，28，9： | 12 |
| 10 | Relations between sedimentary sequence and paleoclimatic changes during last 200 ka in the southern South China Sea显示文摘Core NS-93-5 was taken from the mild slope terrace of the southern South China Sea (SCS), which has preserved the steadiness depositional record of the normal marine environment since late Quaternary. Sedimentary sequence and oxygen isotopic stratigraphy of high resolution in the near 200 ka of the southern SCS has been established. By the comparative analysis with GISP2’s ice core, the depositional record of D/O’s events 1-21 and Heinrich’s events H1-H6 in the southern SCS that reflected the quick climate change in short time scale since the last interglacial stage is revealed, which indicates that in the last 200 ka in the southern SCS and the Arctic area there was tele-connection of paleoclimate and the unstability of the Western Pacific Warm Pool. This note shows that the sedimentary layers order of this sea area classified by color identification and by oxygen isotope stratum has the very good corresponding relation. The color feature of sediment changes along with the climate and the former | Muhong Chen Xia Tu Fan Zheng Wen Yan Xianzan Tang Jun Lu Baogui Wang Miao’an Lu | 2000 | Chinese Science Bulletin2000，45，14： | 12 |
| 11 | Hyperinsulinemia,Insulin Resistance and Cognitive Decline in Older Cohort显示文摘Objective Type 2 diabetes has been recently recognized as an important risk factor for cognitive decline of patients with Alzheimer's disease (AD).But the roles of hyperinsulinemia (HI) and insulin resistance (IR) in the development of AD are still controversial.This study was designed to evaluate whether HI or IR influenced the cognitive functions of older cohort.Methods The cognitive functions of 328 consecutive elderly patients were evaluated with a battery of cognitive rating scales.Their fasting blood glucose (FBG) and fasting insulin (FINS) were analyzed and IR was calculated with modified‐Homa.The cognitive scores in different groups and the correlation of cognitive functions with HI or IR were analyzed.Results In our study,there were 180 participants with HI and 148 without HI,and 192 with IR and 136 without IR.The participants with HI showed worse cognitive functions than those without HI in MMSE,MOCA,CDR,orientation,delayed memory,and attention/calculation domains.Similarly,the elderly with IR had lower cognitive scores than those without IR in MMSE,MOCA,CDR,GDS,orientation,delayed memory,and attention/calculation domains.The insulin levels and Homa IR had negative correlation with the scores of MMSE and delayed memory,not only in the model 1 adjusted for FBG and diabetes history,but also in the model 2 adjusted for all nine demographic characteristics.Conclusion HI and IR are important risk factors for cognitive decline of the elderly,especially for the dysfunctions in delayed memory domains. | ZHONG Yuan MIAO Ya JIA Wei Ping YAN Hong WANG Bei Yun JIN Jun | 2012 | Biomedical and Environmental Sciences2012，25，1： | 11 |
| 12 | Bone physiological microenvironment and healing mechanism:Basis for future bone-tissue engineering scaffolds显示文摘Bone-tissue defects affect millions of people worldwide.Despite being common treatment approaches,autologous and allogeneic bone grafting have not achieved the ideal therapeutic effect.This has prompted researchers to explore novel bone-regeneration methods.In recent decades,the development of bone tissue engineering(BTE)scaffolds has been leading the forefront of this field.As researchers have provided deep insights into bone physiology and the bone-healing mechanism,various biomimicking and bioinspired BTE scaffolds have been reported.Now it is necessary to review the progress of natural bone physiology and bone healing mechanism,which will provide more valuable enlightenments for researchers in this field.This work details the physiological microenvironment of the natural bone tissue,bone-healing process,and various biomolecules involved therein.Next,according to the bone physiological microenvironment and the delivery of bioactive factors based on the bone-healing mechanism,it elaborates the biomimetic design of a scaffold,highlighting the designing of BTE scaffolds according to bone biology and providing the rationale for designing next-generation BTE scaffolds that conform to natural bone healing and regeneration. | Guanyin Zhu Tianxu Zhang Miao Chen Ke Yao Xinqi Huang Bo Zhang Yazhen Li Jun Liu Yunbing Wang Zhihe Zhao | 2021 | Bioactive Materials2021，6，11： | 11 |
| 13 | Plasma microRNAs as potential new biomarkers for early detection of early gastric cancer显示文摘BACKGROUND Early gastric cancer(EGC), compared with advanced gastric cancer(AGC), has a higher 5-year survival rate. However, due to the lack of typical symptoms and the difficulty in diagnosing EGC, no effective biomarkers exist for the detection of EGC, and gastroscopy is the only detection method.AIM To provide new biomarkers with high specificity and sensitivity through analyzed the differentially expressed microRNAs(miRNAs) in EGC and AGC and compared them with those in benign gastritis(BG).METHODSWe examined the differentially expressed miRNAs in the plasma of 30 patients with EGC, AGC, and BG by miRNA chip analysis. Then, we analyzed and selected the significantly different miRNAs using bioinformatics. Reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)confirmed the relative transcription level of these miRNAs in another 122 patients, including patients with EGC, AGC, Helicobacter pylori(H. pylori)-negative gastritis(Control-1), and H. pylori-positive atrophic gastritis(Control-2).To establish a diagnostic model for the detection of plasma miRNA in EGC, we chose miRNAs that can be used to determine EGC and AGC from Control-1 and Control-2 and miRNAs in EGC from all other groups.RESULTS Among the expression profiles of the miRNA chips in the three groups in the discovery set, of 117 aberrantly expressed miRNAs, 30 confirmed target prediction, whereas 14 were included as potential miRNAs. The RT-qPCR results showed that 14 potential miRNAs expression profiles in the two groups exhibited no differences in terms of H. pylori-negative gastritis(Control-1) and H. pyloripositive atrophic gastritis(Control-2). Hence, these two groups were incorporated into the Control group. A combination of four types of miRNAs,miR-7641, miR-425-5 p, miR-1180-3 p and miR-122-5 p, were used to effectively distinguish the Cancer group(EGC + AGC) from the Control group [area under the curve(AUC) = 0.799, 95% confidence interval(CI): 0.691-0.908, P < 0.001].Additionally, miR-425-5 p, miR-24-3 p, miR-1180-3 p and miR-122-5 p were utilized to distinguish EGC from the Control group(AUC = 0.829, 95%CI: 0.657-1.000, P =0.001). Moreover, the miR-24-3 p expression level in EGC was lower than that in the AGC(AUC = 0.782, 95%CI: 0.571-0.993, P = 0.029), and the miR-4632-5 p expression level in EGC was significantly higher than that in AGC(AUC = 0.791,95%CI: 0.574-1.000, P = 0.024).CONCLUSION The differentially expressed circulatory plasma miR-425-5 p, miR-1180-3 p, miR-122-5 p, miR-24-3 p and miR-4632-5 p can be regarded as a new potential biomarker panel for the diagnosis of EGC. The prediction and early diagnosis of EGC can be considerably facilitated by combining gastroscopy with the use of these miRNA biomarkers, thereby optimizing the strategy for effective detection of EGC. Nevertheless, larger-scale human experiments are still required to confirm our findings. | Xiao-Liang Zhu Long-Fei Ren Hai-Ping Wang Zhong-Tian Bai Lei Zhang Wen-Bo Meng Ke-Xiang Zhu Fang-Hui Ding Long Miao Jun Yan Yan-Ping Wang Yu-Qin Liu Wen-Ce Zhou Xun Li | 2019 | World Journal of Gastroenterology2019，25，13： | 11 |
| 14 | State-of-the-art review of the development and application of bridge rotation construction methods in China显示文摘This work provides a comprehensive review of the development and applications of bridge rotation construction methods(BRMs) and related critical construction technologies in China. A brief history of BRMs and an outline of their categories,including the rotating structural system, are given first. Subsequently, a discussion on the recent practice of BRMs in China is carried out from three types of bridges:(1) continuous beam and rigid frame bridges;(2) arch bridges;(3) cable-stayed bridges.For each group, a survey of the vital bridge cases that have adopted BRMs in China in the past two decades is provided. Key parameters, including the structure weight of rotation, maximum cantilever span, and rotation duration, are also compared and discussed. Afterward, the principles and applications of the weighing test and the spherical hinge stability, which are both related to the safety of the horizontal rotation construction process, are analyzed in the paper. Additionally, the limitations of BRMs are presented and discussed, and the article concludes with a summary of the current use and future trend of BRMs. The information and experience of BRMs provided in this work can be referenced by engineers and researchers who are devoted to the construction of bridges. | SU Miao WANG Jun PENG Hui CAI CS DAI GongLian | 2021 | Science China(Technological Sciences)2021，64，6： | 9 |
| 15 | Correlation of serum vitamin D, adipose tissue vitamin D receptor, and peroxisome proliferator-activated receptor γ in women with gestational diabetes mellitus显示文摘Background:Gestational diabetes mellitus(GDM)is a common complication during pregnancy.Obesity and overweight are closely related to metabolic diseases and diabetes.However,the role of adipose tissue in the pathogenesis of GDM remains to be studied.The aim of this study was to investigate the correlation of vitamin D(VD)levels,VD receptor(VDR),and peroxisome proliferatoractivated receptor g(PPARg)expression with GDM in overweight or obese women.Methods:One hundred and forty pregnant women with full-term single-birth cesarean-section were selected as the study subjects and grouped(70 GDM women,including 35 non-overweight/non-obese women[group G1]and 35 women with overweight or obesity[group G2];70 pregnant women with normal glucose tolerance,including 35 non-overweight/non-obese women[group N1]and 35 overweight/obese women[group N2]).The levels of serum VD,blood biochemistry,and adiponectin were compared in these women.Subcutaneous adipose tissue was isolated from the abdominal wall incision.VDR and PPARg messenger RNA(mRNA)transcript levels in these adipose tissues were quantified by real-time polymerase chain reaction.The differences between the levels of PPARg protein and phosphorylated PPARg Ser273 were detected by Western blotting.Results:The serum VD level ofGDM women was lower in comparison to that of women with normal glucose tolerance(G1 vs.N1:20.62±7.87 ng/mL vs.25.85±7.29 ng/mL,G2 vs.N2:17.06±6.74 ng/mL vs.21.62±7.18 ng/mL,P<0.05),and the lowest in overweight/obese GDM women.VDR and PPARg mRNA expression was higher in the adipose tissues of GDM women in comparison to that of women with normal glucose tolerance(VDR mRNA:G1 vs.N1:210.00[90.58–311.46]vs.89.34[63.74–159.92],G2 vs.N2:298.67[170.84–451.25]vs.198.28[119.46–261.23],PPARg mRNA:G1 vs.N1:100.72[88.61–123.87]vs.87.52[66.37–100.04],G2 vs.N2:117.33[100.08–149.00]vs.89.90[76.95–109.09],P<0.05),and their expression was the highest in GDM+overweight/obese women.VDR mRNA levels positively correlated with the pre-pregnancy body mass index(BMI),pre-delivery BMI,fasting blood glucose(FBG),homeostasis model assessment of insulin resistance(HOMA-IR),and PPARg mRNA while it negatively correlated with the VD and the adiponectin levels(r=0.395,0.336,0.240,0.190,0.235,–0.350,–0.294,respectively,P<0.05).The degree of PPARg Ser273 phosphorylation increased in obese and GDM pregnant women.PPARg mRNA levels positively correlated with pre-pregnancy BMI,pre-delivery BMI,FBG,HOMA-IR,serum total cholesterol,triglyceride,free fatty acid,and VDR mRNA,while it negatively correlated with the VD and adiponectin levels(r=0.276,0.199,0.210,0.230,0.182,0.214,0.270,0.235,–0.232,–0.199,respectively,P<0.05).Conclusions:Both GDM and overweight/obese women had decreased serum VD levels and up-regulated VDR and PPARg mRNA expression in adipose tissue,which was further higher in the overweight or obese women with GDM.VDmay regulate the formation and differentiation of adipocytes through the VDR and PPARg pathways and participate in the occurrence of GDM. | Hui-Yan Wang Guang-Tong She Li-Zhou Sun Hao Lu Yin-Pin Wang Jun Miao Ke-Zhuo Liu Cai-Feng Sun Hui-Hui Ju | 2019 | Chinese Medical Journal2019，，21： | 9 |
| 16 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 17 | Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties显示文摘TaPHT1.2 is a functional,root predominantly expressed and low phosphate(Pi) inducible high-affinity Pi transporter in wheat,which is more abundant in the roots of P-efficient wheat genotypes(e.g.,Xiaoyan 54) than in P-inefficient genotypes(e.g.,Jing 411) under both Pi-deficient and Pi-sufficient conditions.To characterize TaPHT1.2 further,we genetically mapped a TaPHT1.2 transporter,TaPHT1.2-D1,on the long arm of chromosome 4D using a recombinant inbred line population derived from Xiaoyan 54 and Jing 411,and isolated a 1,302 bp fragment of the TaPHT1.2-D1 promoter(PrTaPHT1.2-D1) from Xiaoyan 54.TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediate the translocation of Pi from roots to shoots.PrTaPHT1.2-D contains a number of Pi-starvation responsive elements,including P1BS,WRKY-binding W-box,and helix-loop-helix-binding elements.PrTaPHT1.2-D1 was then used to drive expression of β-glucuronidase(GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation.Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots.As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411,and this SNP does not exist within the Pi-starvation responsive elements,the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 may not be caused by this SNP. | Jun Miao Jinghan Sun Dongcheng Liu Bin Li Aimin Zhang Zhensheng Li Yiping Tong | 2009 | Journal of Genetics and Genomics2009，36，8： | 8 |
| 18 | Reaction decoupling in thermochemical fuel conversion and technical progress based on decoupling using fluidized bed显示文摘Thermochemical conversion of fuels via pyrolysis/carbonization,cracking,gasification and combustion has to involve a number of individual reactions called attribution reactions to form an intercorrelated reaction network for any conversion process.By separating one or some attribution reactions from the others to decouple their interactions existing in the reaction network,the so-called reaction decoupling enables a better understanding of the complex thermal conversion process and further the optimization of the conditions for attribution reactions as well as the entire conversion process to realize advanced performances.The dual bed conversion and two-stage conversion are the two representative types of fuel conversion technologies developed in recent years based on reaction decoupling.Many technical advantages have been proven for such decoupling fuel conversion technologies,such as poly-generation of products,low-cost production of high-grade products,elimination of undesirable products or pollutants,easy operation and control,and so on.The treated fuels with decoupling conversion technologies mainly include solid biomass and coal,as well as liquid petroleum oil.This paper is devoted to reiteration of the reaction decoupling concept and further to reviewing the research,developments and successful applications of several decoupling fuel conversion technologies of two such types by using fluidized bed as their major reactors. | Zhennan Han Sulong Geng Xi Zeng Shipei Xu a Ping An Jiguang Cheng Jun Yang Feng Li Suyi Zhang Miao Liu Guoqing Guan Guangwen Xu | 2018 | Carbon Resources Conversion2018，1，2： | 8 |
| 19 | Analysis of ESTs and gene expression patterns of the posterior silkgland in the fifth instar larvae of silkworm, Bombyx mori L.显示文摘The fibroin gene expression pattern and regulation of the posterior silkgland were studied by means of expressed sequence tags (ESTs) using the first and fifth day larvae of the fifth instar of silkworm, Bombyx mori L (strain: C 108). The results showed that there were 911 repetitive ESTs and 1950 single sequences (Singlets) among total 2861 consentient sequences, which were spliced. 1335 sequences were identified and the other 1526 were unknown. 5560 sequences (55.89%) in the posterior silkgland cell of the silkworm were new ESTs without homology with EST data published by Mita et al. The number of repetitive ESTs and single sequences from the first day larvae of the fifth instar was double more than that of the fifth day of the same instar in the silkworms. The unigenes which were more than 50 in repetitive EST size (contig size) came to only about 0.5% in total consentient sequences. There were significant differences between gene expression frequencies, and expressed genes were related to fibroin synthesis and its secretion and fibroin composition. Comparing the fifth day with the first day of the fifth instar, the genes-expressed quantity of fibroin heavy-chain gene was 18 fold higher, fibroin light-chain gene 9 fold and fibroin P52 gene 8 fold. 508 genes functioned for cellular component and 315 for enzyme after function tracing. These results implied that the gene expression of the first day was mainly for preparation for fibroin synthesis except for the growth of silkgland cells, and the gene expression of the fifth day of the fifth instar was mainly for synthesizing and excreting fibroin. Because the ratio of heavy chain, light chain and p25 of fibroin was not 6:6:1 as theoretically expected, or its special H-chain structure, the H-chain gene was not easy to detect through EST technique. Most of genes among total 2861 consentient sequences functioned for fibroin synthesis and secretion. This suggested the fibroin synthesis and secretion procedure of the posterior silkgland was more complex than the knowledge we have. | ZHONG Boxiong, YU Yingpo, XU Yusong, YU Hong, LU Xingmeng, MIAO Yungen, YANG Jun, XU Hao, HU Songnian & LOU Chengfu College of Animal Sciences, Zhejiang University, Hangzhou 310029, China Hangzhou Huada Gene Research and Development Center, Southern Base of Genomic Information, Chinese Academy of Sciences, Hangzhou 310007, China | 2005 | Science China(Life Sciences)2005，48，1： | 8 |
| 20 | Inhibition of squamous cancer growth in a mouse model by Staphylococcal enterotoxin B-triggered Th9 cell expansion显示文摘当前,为有鳞的癌症(SqC ) 的治疗是不能令人满意的。Staphylococcal enterotoxin B (SEB ) 举办强壮的有免疫力的规章的活动。这研究测试 SEB 强制的假设在一个老鼠模型的 SqC 生长上的免疫疗法的效果。C3H/HeN 老鼠和 SqC 房间衬里有鳞的房间癌 VII 被用来创造一个 SqC 老鼠模型。有免疫力的房间评价被流动 cytometry 执行。实时 RT-PCR 并且西方的弄污被用来计算目标分子表达式。apoptosis 试金被用来估计 T helper-9 (Th9 ) 的镇压效果 SqC 房间上的房间。结果证明显著地由 SEB 正 SqC 抗原组成的免疫疗法在老鼠禁止了 SqC 生长。Th9 房间的频率显著地在治疗以后在 SqC 组织和老鼠脾被增加。SEB 显著地增加了信号变换器的层次和抄写 5 phosphorylation 和 histone deacetylase-1 (HDAC1 ) 和 PU.1 的表示的使活跃之物(interleukin 的抄写因素 9 (IL-9 ) 基因) 在 CD4 + T 房间。到 SqC 特定的 Th9 房间的暴露显著地在 vitro 并且在 vivo 导致了 SqC 房间 apoptosis。在结论, SEB 的管理在忍受 SqC 老鼠,和 theseTh9 细胞导致 Th9 细胞禁止 SqC 生长。 | Bei-Ping Miao Rui-Shi Zhang Huan-Ji Sun Yan-Ping Yu Tao Chen Lin-Jing Li Jiang-Qi Liu Jun Liu Hai-Qiong Yu Min Zhang Zhi-Gang Liu Ping-Chang Yang | 2017 | Cellular & Molecular Immunology2017，14，4： | 8 |
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