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| 1 | 青蟹陆基高密度循环水养殖技术的建立与应用显示文摘拟穴青蟹俗称青蟹,属甲壳纲、十足目、梭子蟹科、青蟹属,广泛分布于我国长江口及以南沿海海域,是我国具有重要经济价值的海洋渔业资源和海水养殖品种之一。该品种具有生长速度快、肉质鲜美、营养价值高等特点,素有'海中人参'之美誉,在国内外市场中供不应求,深受消费者欢迎。青蟹在我国已有100多年的养殖历史,在市场价值驱动下,渔民的养殖热情非常高涨,养殖规模居高不下。据《2018中国渔业统计年鉴》记录,我国的青蟹养殖面积接近35万亩,年养殖产量超过15万吨,而广东省的养殖产量占全国1/3以上。 | 马洪雨 谭华强 方少彬 黄智 沈卓辉 沈卓坤 张建生 张健彬 林锋 吴清洋 Mhd Ikhwanuddin | 2019 | 科学养鱼2019，41，12： | 4 |
| 2 | 罗氏沼虾蜕皮过程中释放的氨基酸组分是引发同类残杀的因素?显示文摘目的:确定罗氏沼虾在蜕皮过程(该过程引起同类残杀)中释放的氨基酸组分,并明确其中差异的氨基酸成分。创新点:了解罗氏沼虾蜕皮过程中生化组分的变化对于理解同类残杀的重要意义。目前,还没有关于同类残杀和蜕皮过程与生化组分之间关系的研究报道。本研究以此为切入点,采用统计学方法探究二者之间的关联,并鉴定其中差异的氨基酸成分。方法:在人工饲养条件下,测定了蜕皮阶段和非蜕皮阶段的罗氏沼虾肌肉、外骨骼和养殖水体中的总氨基酸及自由氨基酸组分,采用双因素方差分析法比较分析了两个阶段氨基酸组分的差异,同时鉴定了差异的氨基酸成分。结论:在蜕皮阶段,组织中的总氨基酸以脯氨酸和肌氨酸的含量最高,而水体中的自由氨基酸含量以色氨酸和脯氨酸为主。研究结果表明,这些氨基酸组分在罗氏沼虾蜕皮过程中释放了促进同类残杀的化学信号。 | Abu Seman JUNETA-NOR Noordiyana Mat NOORDIN Mohamad Nor AZRA Hong-yu MA Norainy Mohd HUSIN Mhd IKHWANUDDIN | 2020 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2020，21，10： | 2 |
| 3 | Myo-inositol accelerates the metamorphosis from megalopa to crablet of Scylla paramamosain by modulating cuticle decomposition and reconstruction显示文摘The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic animals.In the present study,S.paramamosain megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be affected.The results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,respectively.To decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with myo-inositol.The integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of cuticle.qPCR results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related pathways.Considering the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of S.paramamosain.Besides,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar metabolism.It implies that myo-inositol may improve survival by regulating energy or nutrient absorption.Additionally,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of S.paramamosain.Overall,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival. | Wenxiao Cui Hafiz Sohaib Ahmed Saqib Weifeng Gao Ruibo Wang Yang He Yang Yu Zhen Lin Qingyu Zhang Yin Zhang Shengkang Li Huaiping Zheng Yueling Zhang Mhd Ikhwanuddin Hongyu Ma | 2023 | Aquaculture and Fisheries2023，8，6： | 0 |
| 4 | Identification and Characterization of Gene SpDMRT99B and Its Sex-Biased Expression Profile in the Mud Crab,Scylla paramamosain显示文摘The doublesex and mab-3 related transcription factor(DMRT)gene family is conserved from invertebrates to humans.The functions of DMRT are mainly involved in sex development and the formation of many tissues and organs.In this study,a DM(Doublesex/Mab-3-domain gene was identified in the mud crab Scylla paramamosain,and was named SpDMRT99B because of its many similarities to arthropod DMRT99B and phylogenetically close relationship with arthropod DMRT99B.The cDNA of SpDMRT99B gene is 1249 bp in length,encoding 224 amino acids.From 254 bp to 928 bp there is a conserved DM domain.No transmembrane domain was identified.Through multiple amino acid alignment and phylogenetic tree analysis,the closest gene to SpDMRT99B is Eriocheir sinensis DMRT99B,followed by Macrobrachium rosenbergii and Drosophila melanogaster DMRT99B.The expressions of the gene were characterized in different tissues of female and male crabs during early development period of crab individuals,as well as in different development periods of gonads.The results showed that SpDMRT99B gene is significantly highly expressed in testis than in ovary and other tissues.The expression level of SpDMRT99B in testis at different stages is significantly higher than that in ovary,and it is particularly highly expressed in immature testis.In early developmental stages of larvae,the expressions of SpDMRT99B remain at a low level and reach a peak at zoea stage I when the body segments shape up.It is speculated that SpDMRT99B gene might be involved in the gonadal development process and somitogenesis of S.paramamosain. | ZHANG Yin FANG Shaobin LIN Fei LI Shengkang ZHENG Huaiping ZHANG Yueling IKHWANUDDIN Mhd MA Hongyu | 2021 | Journal of Ocean University of China2021，20，6： | 0 |
| 5 | A review of gynogenesis manipulation in aquatic animals显示文摘Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase the market size and profitable.This article discusses on the protocol applied to produce gynogens progeny and the successful rate of gynogenesis production in fishes,molluscs and aquatic crustaceans of shrimps in general.Overall most of the UV length applied for irradiated the sperm were around 254-365 nm for(20-40 s),(20-80 s)and(5-8 s)for shrimps,254 nm,30 s for molluscs species and for fishes were around 254 nm for(1.5min)and(2-12 min)time of exposure respectively.For gynogenesis induction,the fertilized eggs were treated with cold shock,heat shock or cytochalasin-B for both shrimp and fishes gynogens technique.Fertilization rate was identified around 4.33%-19.67%in shrimp.Successful hatching rate was identified around 3.0%,14.9%-37.2%of gynogens offspring in shrimp and various percentages of hatching rates were identified from each species of fish gynogens.Overall,there is still low survival rate of gynogens produced using gynogenesis technique and further study should be carried out to improve the gynogens production.The discussed protocols serve as a guide lines for the gynogenesis technique application of all female monosex culture in the future. | Hidayah Manan A.B.Noor Hidayati Nur Aina Lyana Adnan Amin-Safwan Hongyu Ma Nor Azman Kasan Mhd Ikhwanuddin | 2022 | Aquaculture and Fisheries2022，7，1： | 0 |
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