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| 1 | Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi显示文摘AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8).METHODS: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooksone of which was connected to a force transducer for the measurement of isometric tension. The experiments were carried out in a thermostatically controlled (37±0.2℃)organ bath (5 mL) containing Krebs solution. The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1μmol/L) were evaluated in the presence and absence of N^G-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100μmol/L), and (p-chloro-D-Phe^6-Leu^17)-VIP (VIPa,30μmol/L), a VIP receptor antagonist.RESULTS: CCK-8 stimulated the phasic activity of the SO.NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension.Pre-incubation with VIPa also attenuated this CCK-8 effect.The combined application of LNNA and VIPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8.CONCLUSION: VIP and NO together contribute to an increase in phasic activity of SO. | Attila Pálv(o|¨)lgyi Réka Sári József Németh Annamária Szabolcs István Nagy Péter Hegyi János Lonovics Zoltán Szilvássy | 2005 | World Journal of Gastroenterology2005，11，21： | 11 |
| 2 | Relevance of α-defensins(HNP1-3) and defensin β-1 in diabetes显示文摘AIM: To investigate the genetic background of human defensin expression in type 1 and 2 diabetes.METHODS: Associations between DEFA1/DEFA3 gene copy number polymorphism and diabetes as well as between the promoter polymorphisms of DEFB1 and diabetes were studied. The copy number variation of the DEFA1/DEFA3 genes was determined in 257 diabetic patients(117 patients with type 1 and 140 with type 2 diabetes). The control group consisted of 221 age- and gender-matched healthy blood donors. The cumulative copy numbers of the DEFA1/DEFA3 genes were detected by using quantitative PCR analysis. To evaluate the HNP 1-3(human neutrophil peptide 1-3 or α-defensin) levels in the circulation, plasma HNP 1-3 concentrations were measured by ELISA. The expression of DEFA1/A3 in peripheral leukocytes of the diabetic patients was measured by quantitative RT PCR analysis. Three SNPs of the human DEFB1(human defensin β-1) gene: DEFB1 G-20A(rs11362), DEFB1 C-44G(rs1800972) and DEFB1 G-52A(rs1799946) were genotyped by Custom TaqMan? Real Time PCR assay.RESULTS: Significant differences were observed in HNP1-3 levels between the healthy subjects and both groups of diabetic patients. The mean ± SE was 28.78 ± 4.2 ng/mL in type 1 diabetes, and 29.82 ± 5.36 ng/mL in type 2 diabetes, vs 11.94 ± 2.96 ng/mL in controls; P < 0.01 respectively. There was no significant difference between patients with type 1 and type 2 diabetes in the high plasma concentrations of HNP1-3. The highest concentrations of α-defensin were found in diabetic patients with nephropathy(49.4 ± 4.8 ng/mL), neuropathy(38.7 ± 4.8 ng/mL) or cardiovascular complications(45.6 ± 1.45 ng/L). There was no significant difference in the cumulative copy numbers of DEFA1/DEFA3 genes between controls and patients, or between patients with the two types of diabetes. Comparisons of HNP 1-3 plasma level and DEFA1/A3 copy number of the same patient did not reveal significant relationship between defensin-α levels and the gene copy numbers(r2 = 0.01). Similarly, no positive correlation was observed between the copy numbers and the mRNA expression levels of DEFA1/A3. Regarding the C-44G polymorphism of DEFB1, the GG 'protective' genotype was much less frequent(1%-2%) among both groups of patients than among controls(9%).CONCLUSION: Elevated HNP1-3 levels in diabetes are independent of DEFA1/DEFA3 copy numbers, but GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin β-1 production. | Balázs Csaba Németh Tamás Várkonyi Ferenc Somogyvári Csaba Lengyel Katalin Fehértemplomi Szabolcs Nyiraty Péter Kempler Yvette Mándi | 2014 | World Journal of Gastroenterology2014，20，27： | 4 |
| 3 | Antioxidant potential,tannin and polyphenol contents of seed and pericarp of three Coffea species显示文摘Objective:To investigate the antioxidant activity,total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis(C.benghalensis) and Coffea liberica compared to Coffea arabica(C.arabica).Methods:The antioxidant potential,total tannin and polyphenol contents of the immature and mature seed and pericarp of C.benghalensis and Coffea liberica were quantified and compared to C.arabica.Enhanced chemiluminescence(ECL).2,2-diphenyl-1-picrylhydruzyl(DPPH).oxygen radical absorbance capacity,Folin-Ciocaltcau method and total tannin content assays were used.Results:Trolox equivalent(TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation(r^2=0.587)while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant.A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique(r^2=0.610 7) in each species,however the immature pericarp of C.benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay.The immature pericarp of C.benghalensis expressed the highest tannin and polyphenol content,and a high polyphenol level was also detected in the immature seed of C.arabica.The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.Conclusions:The obtained data highlight the potential role of C.benghalensis as a new source of natural antioxidants and polyphenols compared to C.arabica. | va Brigitta Patay Nikolett Sali Tams Kszegi Rita Csepregi Viktória Lilla Blaz Tibor Sebastian Németh Tibor Németh Nóra Papp | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 3 |
| 4 | Relationship between diabetes mellitus and cataract in Hungary显示文摘AIM:To examine the coexistence of diabetes mellitus(DM)and cataract in Hungary.The effects of DM on the cataract surgical results were also in the focus of analysis.METHODS:Statistical data analysis of the results of the Rapid Assessment of Avoidable Blindness with Diabetic Retinopathy(RAAB+DR)module conducted in Hungary in 2015.This cross-sectional,population-based,national survey included 3523 people aged 50 years and over.Participants of the survey were examined on-site.Visual acuity,main cause for visual impairment(using direct and indirect ophthalmoscopes),in case of best corrected visual acuity(BCVA)≤0.5 and blood glucose level(random test with glucometer)were examined.RESULTS:The prevalence of cataract was 23.4%,and DM was 20.0%.The occurrence of cataract steadily increased with age.Among the examined participants with DM,the prevalence of cataract was significantly(P=0.012)higher(+35%)than that in non-diabetic subjects(29.5%vs 21.8%).Following aging(OR=15.2%,P<0.001),DM proved to be the most independent influencing risk factor(OR=49.9%,P<0.001).The presence of DM was neither an influencing factor for complications of cataract surgery,nor for postoperative visual acuity.CONCLUSION:DM appears to be one of the main risk factors for developing cataract.Other risk factors,such as age,sex and environment also play an influencing role.Diabetes does not seem to affect the occurrence of cataract surgical complications. | Anita Pék Dorottya Szabó Gábor LászlóSándor Gábor Tóth András Papp Zoltán Zsolt Nagy Hans Limburg János Németh | 2020 | International Journal of Ophthalmology(English edition)2020，13，5： | 2 |
| 5 | Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes 显示文摘 | Methe H Brunner S Wiegand D | 2005 | J Am Coil Cardiol2005，45，12： | 1 |
| 6 | Creation and characterization of n- alkyhhiol and n- alkylamine self- assembled monolayers on 316L stainless steel 显示文摘 | Ruan C M Bayer T Meth S | 2002 | Thin Solid Films2002，419，12： | 1 |
| 7 | Capsaicin-sensitive local sensory innervation is involved in pacing-induced preconditioning in rat hearts: role of nitric oxide and CGRP?显示文摘 | P. Ferdinandy Tamás Csont Csaba Csonka Marianna T?r?k Mária Dux József Németh László I. Horváth László Dux Zoltán Szilvássy Gábor Jancsó | 1997 | Naunyn - Schmiedeberg’s Archives of Pharmacology1997，，3： | 1 |
| 8 | Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes显示文摘 | Brunner S Wiegand D | 2005 | J Am Coll Cardiol2005，45，： | 1 |
| 9 | Corrosion studies of stainless steel protected by a TiO2 thin film deposited on a sulfonate-functionalized self-assembled monolayer显示文摘 | Meth S Savchenko N Kohypin M | 2010 | Corrosion Science2010，52，1： | 1 |
| 10 | Enhanced T-help- er-1 lymphocyte activation patterns in acute coronary syn- dromes显示文摘 | Methe H Brunner S Wiegand D | 2005 | J Am Colt Cardiol2005，45，12： | 1 |
| 11 | Gustatory perception alterations in obesity: An fMRI study显示文摘 | Csaba Szalay Mihály Aradi Attila Schwarcz Gergely Orsi Gábor Perlaki Lívia Németh Sophia Hanna Gábor Takács István Szabó László Bajnok András Vereczkei Tamás Dóczi József Janszky Sámuel Komoly Péter ?rs Horváth László Lénárd Zoltán Karadi | 2012 | Brain Research2012，，： | 1 |
| 12 | Expansion of circulating TLR4-positive monocytes in patients with ACS 显示文摘 | METHE H KIM J O KOFLER S | 2005 | Circulation2005，111，： | 1 |
| 13 | Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes显示文摘 | METHE H BRUNNER S WIEGAND D | 2005 | J Am Coll Cardiol2005，45，12： | 1 |
| 14 | Expansion of circulat-ing Toll-like receptor 4-positive monocytes in patientswith acute coronary syndrome显示文摘 | Methe H Kim JO Kofler S | 2005 | Circulation2005，111，20： | 1 |
| 15 | Enhanced T - helper - 1 lymphocyte activation patterns in acute coronary syndromes 显示文摘 | Methe H Brunner S Wiegand D | 2005 | J Am Coil Cardio12005，45，12： | 1 |
| 16 | Aerosol deposition modeling in human airways and alveoli显示文摘 | I. Balásházy I. Németh B. Alf?ldy P.P. Szabó CS. Hegedu?s W. Hofmann J. Pálfalvi I. Fehér Sz. T?r?k | 2000 | Journal of Aerosol Science2000，，： | 1 |
| 17 | A hydrogen-based subsurface microbial community dominated by methanogens显示文摘 | Chapelle F H O'Neill K Bradley P M Methe B A Ciufo S A Knobel L L Lovley D R | 2002 | Nature2002，415，6869： | 1 |
| 18 | Enhanced T-helper-1 lymphocyte activation patterns in acute coronary syndromes显示文摘 | Methe H Brunner S Wiegand D | 2005 | J Am Coll Cardiol2005，45，12： | 1 |
| 19 | Expansion of circulating Toll-like receptor 4-positive monocytes in patients with acute coronary syndrome显示文摘 | Methe H Kim JO Kofler S | 2005 | Circulation2005，111，20： | 1 |
| 20 | The roles of platelet function,thromboxane,blood lipids and nitric oxide in hypertension of children and adolescents显示文摘 | Bereczki C T(u)r S N(e)meth I | 2000 | Prostaglandins Leukot Essent Fatty Acids2000，62，5： | 1 |
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