3篇
您的检索式：作者名="Merin Jose"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4显示文摘Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off. | B Valentin Bhimba DA Agnel Defora Franco Geena Mary Jose Jibi Merin Mathew Elsa Lycias Joel | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，3： | 3 |
| 2 | Current status quo on COVID-19 including chest imaging显示文摘With each day the number coronavirus disease 2019(COVID-19)cases continue to rise rapidly and our imaging knowledge of this disease is expeditiously evolving.The role of chest computed tomography(CT)in the screening or diagnosis of COVID-19 remains the subject of much debate.Despite several months having passed since identifying the disease,and numerous studies related to it,controversy and concern still exists regarding the widespread use of chest CT in the evaluation and management of COVID-19 suspect patients.Several institutes and organizations around the world have released guidelines,recommendations and statements against the use of CT for diagnosing or screening COVID-19 infection and advocating its use only for those cases with a strong clinical suspicion of complication or an alternate diagnosis.However,these guidelines and recommendations are in disagreement with majority of the widely available literature,which strongly favour CT as a pivotal tool in the early diagnosis,management and even follow-up of COVID-19 infection.This article besides comprehensively reviewing the current status quo on COVID-19 disease in general,also writes upon the current consensus statements/recommendations on the use of diagnostic imaging in COVID-19 as well as highlighting the precautions and various disinfection procedures being employed world-wide at the workplace to prevent the spread of infection. | Rishi Philip Mathew Merin Jose Vinayak Jayaram Paul Joy Danny George Maria Joseph TeenaSleeba Ajith Toms | 2020 | World Journal of Radiology2020，12，12： | 1 |
| 3 | 红树林真菌Hypocrea lixii VB1的抗癌及抗菌活性(英文)显示文摘AIM:Mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today.Scientific research throughout the world has found evidence to support the fact that its foliar extracts have great potential against human microbial pathogens.This study highlights the isolation of foliar fungi from Rhizophora mucronata,Avicenna officialis and Avicenna marina.METHOD:It was isolated in Sabouroud's Dextrose Agar and mass cultivation was done in Sabouroud's Dex-trose broth.RESULTS:The ethyl acetate extract showed maximum antibacterial activity which inturn checked for different concentra-tion against bacterial pathogens and anticancer activity for Hep2 and MCF7 cell line in vitro.The DNA was isolated from the fungi and the ITS region of 5.8 s RNA was sequenced and assigned to new species as they are separated from the type strains phylogenetic neighbors by sequence similarities.CONCLUSION:This preliminary screening of fungal endophytes revealed their potential to yield potent bioactive compounds for drug discovery programmes. | B.Valentin Bhimba D.A.Agnel Defora Franco Jibi Merin Mathew Geena Mary Jose Elsa Lycias Joel M.Thangaraj | 2012 | 中国天然药物2012，10，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员