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| 1 | Characterization of hepatic progenitors from human fetal liver during second trimester显示文摘AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule (EpCAM) as a marker from human fetal liver and investigate the expression of human leukocyte antigen (HLA) and their markers associated with hepatic progenitor cells. METHODS: EpCAM +ve cells were isolated using magnetic cell sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 (integrin β1), CD49f (integrin α6) and CD90 (Thy 1) was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ (A, B, C) and class Ⅱ (DR) expression was studied by flow cytometry only. RESULTS: FACS analysis indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA class Ⅰ, albumin and AFP but negative for HLA class Ⅱ (DR) and CD45. RT PCR showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical staining, EpCAM +ve cells were shown positive signals for CK18 and albumin. CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor. | Mekala Subba Rao Aleem Ahmed Khan Nyamath Parveen Mohammed Aejaz Habeeb Chittoor Mohammed Habibullah Gopal Pande | 2008 | World Journal of Gastroenterology2008，14，37： | 16 |
| 2 | Modeling phase equilibria of semiclathrate hydrates of CH_4,CO_2 and N_2 in aqueous solution of tetra-n-butyl ammonium bromide显示文摘Semiclathrate hydrates of tetra-n-butyl ammonium bromide(TBAB) offer potential solution for gas storage,transportation,separation of flue gases and CO2 sequestration.Models for phase equilibria for these systems have not yet been developed in open literatures and thus require urgent attention.In this work,the first attempt has been made to model phase equilibria of semiclathrate hydrates of CH4,CO2 and N2 in aqueous solution of TBAB.A thermodynamic model for gas hydrate system as proposed by Chen and Guo has been extended for semiclathrate hydrates of gases in aqueous solution of TBAB.A correlation for the activity of water relating to the system temperature,concentration of TBAB in the system and the nature of guest gas molecule has been proposed.The model results have been validated against available experimental data on phase equilibria of semiclathrate hydrate systems of aqueous TBAB with different gases as guest molecule.The extended Chen and Guo's model is found to be suitable to explain the promotion effect of TBAB for the studied gaseous system such as,methane,carbon dioxide and nitrogen as a guest molecule.Additionally,a correlation for the increase in equilibrium formation temperature(hydrate promotion temperature,ΔTp) of semiclathrate hydrate system with respect to pure gas hydrate system has been developed and applied to semiclathrate hydrate of TBAB with several gases as guest molecules.The developed correlation is found to predict the promotion effect satisfactorily for the system studied. | Abhishek Joshi Prathyusha Mekala Jitendra S.Sangwai | 2012 | Journal of Natural Gas Chemistry2012，21，4： | 5 |
| 3 | Thinking outside the liver: Induced pluripotent stem cells for hepatic applications显示文摘The discovery of induced pluripotent stem cells (iPSCs) unraveled a mystery in stem cell research, after identification of four re-programming factors for generating pluripotent stem cells without the need of embryos. This breakthrough in generating iPSCs from somatic cells has overcome the ethical issues and immune rejection involved in the use of human embryonic stem cells. Hence, iPSCs form a great potential source for developing disease models, drug toxicity screening and cell-based therapies. These cells have the potential to differentiate into desired cell types, including hepatocytes, under in vitro as well as under in vivo conditions given the proper microenvironment. iPSC-derived hepatocytes could be useful as an unlimited source, which can be utilized in disease modeling, drug toxicity testing and producing autologous cell therapies that would avoid immune rejection and enable correction of gene defects prior to cell transplantation. In this review, we discuss the induction methods, role of reprogramming factors, and characterization of iPSCs, along with hepatocyte differentiation from iPSCs and potential applications. Further, we discuss the location and detection of liver stem cells and their role in liver regeneration. Although tumor formation and genetic mutations are a cause of concern, iPSCs still form a promising source for clinical applications. | Mekala Subba Rao Mitnala Sasikala D Nageshwar Reddy | 2013 | World Journal of Gastroenterology2013，19，22： | 4 |
| 4 | 南瓜种质资源遗传变异、遗传力和遗传进度研究显示文摘对南瓜3个主要栽培种基因型遗传变异性、遗传力和遗传进度进行分析和评价。方差分析结果表明,所研究的所有性状在基因型间差异显著。调查结果显示,所有性状中表型变异系数高于基因型变异系数,表明这些性状的表达受环境调控的影响。其中90天蔓长、果腔大小、单果种粒数、果皮厚度和单蔓结果数的遗传变异系数及表型变异系数最高。遗传力概括估计,果腔最高(84.80%),其次是节间长度(76.90%)和每hm2果实产量(73.60%)。果腔性状遗传进度最高(百分数平均值)。分析结果表明,所有性状的遗传进度变异范围较大(3.44%~72.32%)。因此,可以选择具有优良遗传特征的、具有较高遗传距离的优良基因型选择杂交方案,并识别不同性状的最佳基因,以培育南瓜新的优良杂交品种。 | Mekala Srikanth Bharad S G Thulasiram L B Potdukhe N R 张桂兰(编译) | 2018 | 中国瓜菜2018，31，2： | 4 |
| 5 | Cerium(Ⅳ) ammonium nitrate catalysed facile and efficient synthesis of polyhydroquinoline derivatives through Hantzsch multicomponent condensation显示文摘为 polyhydroquinoline 衍生物的合成的一条一般、方便的实际途径经由一个壶被完成了面对铈(IV ) 的催化数量的芳香的醛, dimedone,乙醇 acetoacetate 和铵醋酸盐的四部件的 Hantzsch 冷凝作用铵硝酸盐(罐头) ,在在周围的温度的乙醇溶剂。简单病情的检查,温和反应条件,便宜、无毒的催化剂,和优秀产品收益是这个方法的有益的特征。 | Cherkupally Sanjeeva Reddy Mekala Raghu | 2008 | Chinese Chemical Letters2008，19，7： | 4 |
| 6 | Physical and degradation properties of PLGA scaffolds fabricated by salt fusion technique显示文摘Tissue engineering scaffolds require a controlled pore size and interconnected pore structures to support the host tissue growth. In the present study, three dimensional (3D) hybrid scaffolds of poly lactic acid (PLA) and poly glycolic acid (PGA) were fabricated using solvent casting/particulate leaching. In this case, partially fused NaCl particles were used as porogen (200-300μ) to improve the overall porosity (≥90%) and internal texture of scaffolds. Differential scanning calorimeter (DSC) analysis of these porous scaffolds revealed a gradual reduction in glass transition temperature (Tg) (from 48°C to 42.5°C) with increase in hydrophilic PGA content. The potential applications of these scaffolds as implants were further tested for their biocompatibility and biodegradability in four simulated body fluid (SBF) types in vitro. Whereas, simulated body fluid (SBF) Type1 with the optimal amount of HCO 3 ions was found to be more appropriate and sensible for testing the bioactivity of scaffolds. Among three combinations of polymer scaffolds, sample B with a ratio of 75:25 of PLA: PGA showed greater stability in body fluids (pH 7.2) with an optimum degradation rate (9% to 12% approx). X-ray diffractogram also confirmed a thin layer of hydroxyapatite deposition over sample B with all SBF types in vitro. | Naveen Kumar Mekala Rama Raju Baadhe Sreenivasa Rao Parcha Prameela Devi Yalavarthy | 2013 | The Journal of Biomedical Research2013，27，4： | 3 |
| 7 | Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats显示文摘 | Vinay Kumar Kondeti Kameswara Rao Badri Dilip Rajasekhar Maddirala Sampath Kumar Mekala Thur Shaik Sameena Fatima Ramesh Babu Kasetti Chippada Appa Rao | 2010 | Food and Chemical Toxicology2010，，5： | 1 |
| 8 | Proteomic analysis of cytoplasmic and surface proteins from yeast cells, hyphae, and biofilms of Candida albicans 显示文摘 | Montserrat M G Perumal P Mekala S | 2009 | Proteomics2009，9，8： | 1 |
| 9 | Modeling and optimization of ultrasound-assisted extraction of polysaccharide from Cucurbita moschata显示文摘 | Maran J P Mekala V Manikandan S | 2013 | Carbohydrate Polymers2013，92，2： | 1 |
| 10 | Cellulase production under solid-state fermentation by Trichoderma reesei RUT C30: statistical optimization of process parameters显示文摘 | Mekala N K Singhania R R Sukumaran R K | 2008 | Appl Bioehem Biotechnol2008，151，： | 1 |
| 11 | Subtypes of dementia:a study from a memory clinic in India显示文摘 | Alladi S Mekala S Chadalawada SK | | 0，，01： | 1 |
| 12 | IL-10-dependent suppression of experimental allergic encephalomyelitis by Th2-differentiated,anti-TCR redirected T lymphocytes显示文摘 | Mekala DJ Alli RS Geiger TL | 2005 | J Immunol2005，174，6： | 1 |
| 13 | Intraneuronal N-acetylaspartate supplies acetyl groups for myelin lipid synthesis:evidence for myelin-associated aspartoacylase显示文摘 | Chak R Aborty G Mekala P | 2001 | Neurochem2001，78，4： | 1 |
| 14 | Effects of recombinant human growth hormone therapy in obesity in adults:a meta analysis显示文摘 | Mekala KC Tritos NA | 2009 | J Clin Endocrinol Metab2009，94，1： | 1 |
| 15 | Cellulase production under solid-state fermentation byTrichoderma reesei RUTC30 statistical optimizationof process parameters 显示文摘 | Mekala NK Singhania RR Sukumaran RK Pandey A | 2008 | ApplBiochem Biotechno2008，151，23： | 1 |
| 16 | IL-10-dependent suppression of experimental allergic encephalo-myelitis by Th2-differentiated,anti-TCR redirected T lymphoeytes显示文摘 | Mekala DJ Alli RS Geiger TL | 2005 | The Journal of Immunology2005，174，6： | 1 |
| 17 | Surveillance for infectious complications in pediatric acute liver failure - a prospective study 显示文摘 | Mekala S Jagadisan B Parija SC | 2014 | Indian J Pediatr2014，82，3： | 1 |
| 18 | Derivation, characterization and retinal differentiation of induced pturipotent stem cells显示文摘 | Mekala SR Vauhini V Nagarajan U | 2013 | J Biosci2013，38，1： | 1 |
| 19 | Effects of recombinant human growth hormone therapy in obesity in adults:a meta analysis显示文摘 | Mekala KC Tritos NA | 2009 | J Clin Endoerinol Metab2009，94，1： | 1 |
| 20 | Intraneuro-nal N - acetylaspartate supplies acetyl groups for myelin lipid syn-thesis :evidence for myelin - associated aspartoacylase 显示文摘 | CHAKRABORTY G MEKALA P YAHYA D | 2001 | J Neu-rochem2001，78，4： | 1 |
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