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| 1 | 附睾炎:基于临床和基础研究的总结启示显示文摘急性附睾炎在泌尿外科门诊的病人中有共同的临床表现。大多数情况下,性传播疾病或者泌尿系的感染都会引起病原菌的感染,并通过泌尿生殖道逆行引发急性附睾炎。虽然保守治疗可能对大多数患者有效并能根除病原菌,但有研究表明这类患者中少精子症甚至无精子症患者升高至40%。为了探讨引起这种现象和随后对精子功能损伤的潜在原因,我们建立了附睾炎的动物模型。研究数据表明附睾管里聚集着异常表达的免疫细胞、免疫调节基因以及病原菌敏感分子。我们推测在附睾头部存在一个耐药微环境,而炎症反应常发生在附睾尾部。这与附睾在不需要降低自身抵抗上行感染能力的前提下,当精子从睾丸中射出后,保护其不受新产生的抗精子抗体损害的观点相一致。然而,严重的炎症反应,特别是在附睾尾部,也可能迁延引发附睾结构和功能的损害。通过对动物模型临床观察研究,我们更好地理解附睾的免疫微环境,附睾炎的易感因素以及合理的治疗,在此基础上减轻附睾炎对附睾的损害,降低这类人群不育的发生率。 | Vera Michel Adrian Pilatz Mark P Hedger Andreas Meinhardt | 2015 | Asian Journal of Andrology2015，17，5： | 16 |
| 2 | Varietal identification of tea(Camellia sinensis)using nanofluidic array of single nucleotide polymorphism(SNP)markers显示文摘Apart from water,tea is the world’s most widely consumed beverage.Tea is produced in more than 50 countries with an annual production of approximately 4.7 million tons.The market segment for specialty tea has been expanding rapidly owing to increased demand,resulting in higher revenues and profits for tea growers and the industry.Accurate varietal identification is critically important to ensure traceability and authentication of premium tea products,which in turn contribute to on-farm conservation of tea genetic diversity.Using a set of single nucleotide polymorphism(SNP)markers developed from the expressed sequence tag(EST)database of Camilla senensis,we genotyped deoxyribonucleic acid(DNA)samples extracted from a diverse group of tea varieties,including both fresh and processed commercial loose-leaf teas.The validation led to the designation of 60 SNPs that unambiguously identified all 40 tested tea varieties with high statistical rigor(p<0.0001).Varietal authenticity and genetic relationships among the analyzed cultivars were further characterized by ordination and Bayesian clustering analysis.These SNP markers,in combination with a high-throughput genotyping protocol,effectively established and verified specific DNA fingerprints for all tested tea varieties.This method provides a powerful tool for variety authentication and quality control for the tea industry.It is also highly useful for the management of tea genetic resources and breeding,where accurate and efficient genotype identification is essential. | Wan-Ping Fang Lyndel W Meinhardt Hua-Wei Tan Lin Zhou Sue Mischke Dapeng Zhang | 2014 | Horticulture Research2014，1，1： | 13 |
| 3 | Exogenous abscisic acid significantly affects proteome in teaplant (Camellia sinensis) exposed to drought stress显示文摘Tea[Camellia sinensis(L.)O.Kuntze]is an important economic crop,and drought is the most important abiotic stress affecting yield and quality.Abscisic acid(ABA)is an important phytohormone responsible for activating drought resistance.Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes,along with crop management practices that can mitigate drought stress.The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.Leaf protein patterns of tea plants under simulated drought stress[(polyethylene glycol(PEG)-treated]and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis(2-DE),followed by matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF)mass spectrometry(MS).Among the 72 protein spots identified by MALDI-TOF MS,16 proteins were downregulated and two were upregulated by exogenous ABA.The upregulated proteins have roles in glycolysis and photosystem II stabilization.Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism,control of reactive oxygen species(ROS),defense,signaling or nucleic acid metabolism.The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress.The results support the importance of the role that ABA plays in the tea plant during drought stress,by improving protein transport,carbon metabolism and expression of resistance proteins. | Lin Zhou Hui Xu Sue Mischke Lyndel W Meinhardt Dapeng Zhang Xujun Zhu Xinghui Li Wanping Fang | 2014 | Horticulture Research2014，1，1： | 12 |
| 4 | Developing single nucleotide polymorphism(SNP)markers from transcriptome sequences for identification of longan(Dimocarpus longan)germplasm显示文摘Longan(Dimocarpus longan Lour.)is an important tropical fruit tree crop.Accurate varietal identification is essential for germplasm management and breeding.Using longan transcriptome sequences from public databases,we developed single nucleotide polymorphism(SNP)markers;validated 60 SNPs in 50 longan germplasm accessions,including cultivated varieties and wild germplasm;and designated 25 SNP markers that unambiguously identified all tested longan varieties with high statistical rigor(P<0.0001).Multiple trees from the same clone were verified and off-type trees were identified.Diversity analysis revealed genetic relationships among analyzed accessions.Cultivated varieties differed significantly from wild populations(Fst=0.300;P<0.001),demonstrating untapped genetic diversity for germplasm conservation and utilization.Within cultivated varieties,apparent differences between varieties from China and those from Thailand and Hawaii indicated geographic patterns of genetic differentiation.These SNP markers provide a powerful tool to manage longan genetic resources and breeding,with accurate and efficient genotype identification. | Boyi Wang Hua-Wei Tan Wanping Fang Lyndel W Meinhardt Sue Mischke Tracie Matsumoto Dapeng Zhang | 2015 | Horticulture Research2015，2，1： | 6 |
| 5 | Developing single nucleotide polymorphism markers for the identification of pineapple(Ananas comosus)germplasm显示文摘Pineapple(Ananas comosus[L.]Merr.)is the third most important tropical fruit in the world after banana and mango.As a crop with vegetative propagation,genetic redundancy is a major challenge for efficient genebank management and in breeding.Using expressed sequence tag and nucleotide sequences from public databases,we developed 213 single nucleotide polymorphism(SNP)markers and validated 96 SNPs by genotyping the United States Department of Agriculture-Agricultural Research Service pineapple germplasm collection,maintained in Hilo,Hawaii.The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection.Twenty-four groups of duplicates were detected,encompassing 130 of the total 170 A cosmos accessions.The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple.Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties.Parentage analysis further revealed that both A.comosus var.bracteatus and A.comosus var.ananassoides are likely progenitors of pineapple cultivars.However,the traditional classification of cultivated pineapple into horticultural groups(e.g.‘Cayenne’,‘Spanish’,‘Queen’)was not well supported by the present study.These SNP markers provide robust and universally comparable DNA fingerprints;thus,they can serve as an efficient genotyping tool to assist pineapple germplasm management,propagation of planting material,and pineapple cultivar protection.The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops. | Lin Zhou Tracie Matsumoto Hua-Wei Tan Lyndel W Meinhardt Sue Mischke Boyi Wang Dapeng Zhang | 2015 | Horticulture Research2015，2，1： | 2 |
| 6 | Adaptive changes of the rat liver cells induced by repeated intraperitoneal injections of d-galactosamine显示文摘 | Hideaki Enzan Brigitte H?berle Karin Meinhardt Christian Schuchhardt Herbert K. Koch Rainer Lesch | 1978 | Virchows Archiv B Cell Pathology1978，，1： | 2 |
| 7 | Multilevel Converters Single-phase Grid Connected Photovoltaic System显示文摘 | Agelidis V G Meinhardt M | 1999 | Solar Energy1999，66，5： | 1 |
| 8 | The testis in immune privilege 显示文摘 | Fijak M Meinhardt A | 2006 | Immunol Rev2006，213，： | 1 |
| 9 | Chemical inte- raction of barium-ealcium-aluminosilieate-based sealing glas- ses with oxidation resistant alloys 显示文摘 | Yang Z G Stevenson J W Meinhardt K D | 2003 | Solid State Ionics2003，160，34： | 1 |
| 10 | Multilevel converters single-phase grid converter for photovohaic system 显示文摘 | Calais M Agelidis V G Meinhardt M | 1999 | Solar energy1999，66，5： | 1 |
| 11 | Chemical stability of glass seal interfaces in intermediate temperature solid oxide fuel cells 显示文摘 | Yang Z G Xia G G Meinhardt K D Well K S | 2004 | J Mater Eng Perform2004，13，3： | 1 |
| 12 | A theory of biological pattern formation显示文摘 | Gierer A Meinhardt H | | 0，，： | 1 |
| 13 | The roles of inhibin and related peptides ingonadal function显示文摘 | De Kretser D M Meinhardt A Meehan T | 2000 | Mol Cell Endocrinol2000，，161： | 1 |
| 14 | Epidemiologic aspect of human cryptosporidiosis and the role of waterborne transmision显示文摘 | Meinhardt PL Casemore PP Miller KB | 1996 | Epidemiol Rev1996，18，2： | 1 |
| 15 | Phase Ⅱ window study on rituximab in newly diagnosed pediatric mature B-cell non-Hodgkin lymphoma and Burkitt leukemia显示文摘 | Meinhardt A Burkhardt B Zimmermann M | 2010 | J Clin Oncol2010，28，： | 1 |
| 16 | Mating types ofAgrocybe aegerita显示文摘 | Meinhardt F Leslie J F | 1982 | Current Genetics1982，，5： | 1 |
| 17 | Multilevel converters single-phase grid connected photovoLltaic system显示文摘 | AGELIDIS V G MEINHARDT M | 1999 | Solar energy1999，66，5： | 1 |
| 18 | Specific determination of trosine-phosphorylated proteins and peptides by differential iodination显示文摘 | MEINHARDT G AUSTERMANN S | 1996 | J Chromatogr A1996，743，2： | 1 |
| 19 | Abeta me-diated diminution of MTT reduction--an artefact ofsingle cell culture显示文摘 | Ronicke R Klemm A Meinhardt J | 2008 | PLoS One2008，3，9： | 1 |
| 20 | The testis in immune privilege显示文摘 | Fijak M Meinhardt A | | 0，，： | 1 |
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