|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice显示文摘AIM:To investigate the role of nicotinamide adenine dinucleotide phosphate(NADPH) oxidase in colon epithelial cells in the pathogenesis of acute and chronic colon inflammation in a mouse model of dextran sulphate sodium(DSS)-induced colitis.METHODS:Balb/c mice were divided into three groups:8 mice with acute DSS-induced colitis(3.5% DSS solution;7 d),8 mice with chronic DSS-induced colitis(3.5% DSS solution for 5 d + water for 6 d;4 cycles;total:44 d) and 12 mice without DSS supplementation as a control group.Primary colonic epithelial cells were isolated using chelation method.The cells were cultivated in the presence of mediators(lipopolysaccharide(LPS),apocynin or diphenyleneiodonium).Viability of cells was assessed by fluorescent microscopy.Production of reactive oxygen species(ROS) by the cells was measured fluorometrically using Amplex Red.Production of tumour necrosis factor-alpha(TNF-α) by the colonic epithelial cells was analysed by ELISA.Nox1 gene expression was assessed by real-time PCR.RESULTS:Our study showed that TNF-α level was increased in unstimulated primary colonic cells both in the acute and chronic colitis groups,whereas decreased viability,increased ROS production,and expression of Nox1 was characteristic only for chronic DSS colitis mice when compared to the controls.The stimulation by LPS increased ROS generation via NADPH oxidase and decreased cell viability in mice with acute colitis.Treatment with NADPH oxidase inhibitors increased cell viability and decreased the levels of ROS and TNF-α in the LPS-treated cells isolated from mice of both acute and chronic colitis groups.CONCLUSION:Our study revealed the importance of NADPH oxidase in the pathogenesis of both acute and chronic inflammation of the colon. | Rima Ramonaite Jurgita Skieceviciene Simonas Juzenas Violeta Salteniene Juozas Kupcinskas Paulius Matusevicius Vilmante Borutaite Limas Kupcinskas | 2014 | World Journal of Gastroenterology2014，20，35： | 4 |
| 2 | Tumor necrosis factor-a,lymphotoxin,linterleukin(IL)6,IL-10,IL-12 and perforin mRNA expression in mononuclear cells in response to acetylcholine receptor is augmented in myasthenia gravis显示文摘 | Matusevicius D Navikas V Palasik W | 1996 | J Neuroimmunol1996，71，： | 1 |
| 3 | Correlation ofdifferent biochemical parameters in blood sera ofhealthy and sick cows显示文摘 | Klimiene Spakauskas Matusevicius | 2005 | Vet Res Commun2005，29，2： | 1 |
| 4 | Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis 显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Mult Scler1999，5，2： | 1 |
| 5 | Altered expression of costimulatory molecules in myasthenia gravis显示文摘 | Teleshova N Matusevicius D Kivisakk P | | 0，，： | 1 |
| 6 | Monocyte-derived cytokines in multiple sclerosis显示文摘 | Filion LG Graziani-Bowering G Matusevicius D | | 0，，02： | 1 |
| 7 | High level of IL-10 secreting cells are present in blood in cerebrovascular diseases显示文摘 | Kostulas N Matusevicius D | 1999 | Eur J Neurol1999，6，4： | 1 |
| 8 | Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis显示文摘 | Matusevicius D Kiviskk P He B | 1999 | Mult Scler1999，5，2： | 1 |
| 9 | Interleukin-17 mRNA ex- pression in blood and CSF mononuclear cells is augmented in multi- ple sclerosis 显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Mult Scler1999，5，2： | 1 |
| 10 | Monocyte-derived cytokines in multiple sclerosis显示文摘 | Filion LG Graziani-Bowering G Matusevicius D | 2003 | Clin Exp Immunol2003，131，2： | 1 |
| 11 | Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Mult Scler1999，5，2： | 1 |
| 12 | 显示文摘 | Murzenok PP Matusevicius D Freedman MS | 2002 | Clin Immunol2002，103，31： | 1 |
| 13 | Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Muh Scler1999，5，2： | 1 |
| 14 | Increased inter- leukin 6 mRNA expression in blood and cerebrospinal fluid from patients with acute Guillain-barre syndrome 显示文摘 | Navikas V Matusevicius D Soderstorm M | 1978 | Ero Neurol1978，17，5： | 1 |
| 15 | Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis 显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Multiple sclerosis ( Houndmills Basingstnke England)1999，5，2： | 1 |
| 16 | Interleukin-12 and perforin mRNA expression is augmented in blood mononuclear cells in multiple sclerosis显示文摘 | MATUSEVICIUS D KIVISAKK P NAVIKAS V | 1998 | Scand J Immunol1998，47，6： | 1 |
| 17 | Tumor necrosis factor-α,lymphotoxin,interleukin(IL)-6,IL-10,IL-1 and perforin mRNA expression monoculear cells in response to acetylcholine receptor is augmented in myasthenia gravis显示文摘 | Matuseviciu D Navikas V | 1996 | J Neuroimmunol1996，71，12： | 1 |
| 18 | Monocyte-derived IL12,CD86(B7-2) and CD40L expression in relapsing and progressive multiple sclerosis显示文摘 | Filion LG Matusevicius D Graziani-Bowering GM | 2003 | Clin Immuno12003，106，2： | 1 |
| 19 | Interlenkin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis显示文摘 | Matusevicius D Kivisakk P He B | 1999 | Mult Seler1999，5，： | 1 |
| 20 | High levels of IL-10 secreting cells are present in blood in cerebrovascular diseases显示文摘 | Dziedic S H Kostulas N Matusevicius D | 1996 | Eur J Nelurol1996，4，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员