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2篇 您的检索式:作者名="Mathieu Ingouff"
    题名 作者 年代 出处 被引量
1Molecular characterization of the anthocyanidin synthase gene in Forsythia × intermedia reveals organ-specific expression during flower development显示文摘Carlo Rosati Alain Cadic Michel Duron Mathieu Ingouff Philippe Simoneau 1999Plant Science1999,,1:1
2High temperature increases centromeremediated genome elimination frequency and enhances haploid induction in Arabidopsis显示文摘Double haploid production is the most effective way to create true-breeding lines in a single generation.In Arabidopsis,haploid induction via mutation of the centromere-specific histone H3(cenH3)has been shown when the mutant is outcrossed to the wild-type,and the wild-type genome remains in the haploid progeny.However,factors that affect haploid induction are still poorly understood.Here,we report that a mutant of the cenH3 assembly factor Kinetochore Null2(KNL2)can be used as a haploid inducer when pollinated by the wild-type.We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold.We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines,suggesting that haploidinducing lines in crops can be identified in a naturally occurring or chemically induced mutant population,avoiding the generic modification(GM)approach at any stage.Furthermore,a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress,even though it did not induce haploids under standard conditions.Thus,we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.Ulkar Ahmadli Manikandan Kalidass Lucie Crhak Khaitova Joerg Fuchs Maria Cuacos Dmitri Demidov Sheng Zuo Jana Pecinkova Martin Mascher Mathieu Ingouff Stefan Heckmann Andreas Houben Karel Riha Inna Lermontova 2023Plant Communications2023,4,3:0
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