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| 1 | Mucosal adhesion and anti-inflammatory effects of Lactobacillus rhamnosus GG in the human colonic mucosa: A proof-of-concept study显示文摘AIM To investigate the adhesion and anti-inflammatory effects of Lactobacil us rhamnosus GG (LGG) in the colonic mucosa of healthy and ulcerative colitis (UC) patients, both in vivo and ex vivo in an organ culture model.METHODS For the ex vivo experiment, a total of 98 patients (68 UC patients and 30 normal subjects) were included. Endoscopic biopsies were collected and incubated with and without LGG or LGG-conditioned media to evaluate the mucosal adhesion and anti-inflammatory effects [reduction of tumor necrosis factor alpha (TNFα) and interleukin (IL)-17 expression] of the bacteria, and extraction of DNA and RNA for quantification by real-time (RT)-PCR occurred after the incubation. A dose-response study was performed by incubating biopsies at 'regular', double and 5 times higher doses of LGG. For the in vivo experiment, a total of 42 patients (20 UC patients and 22 normal controls) were included. Biopsies were taken from the colons of normal subjects who consumed a commercial formulation of LGG for 7 d prior to the colonoscopy,and the adhesion of the bacteria to the colonic mucosa was evaluated by RT-PCR and compared with that of control biopsies from patients who did not consume the formulation. LGG adhesion and TNFα and IL-17 expression were compared between UC patients who consumed a regular or double dose of LGG supplementation prior to colonoscopy.RESULTS In the ex vivo experiment, LGG showed consistent adhesion to the distal and proximal colon in normal subjects and UC patients, with a trend towards higher concentrations in the distal colon, and in UC patients, adhesion was similar in biopsies with active and quiescent inflammation. In addition, bioptic samples from UC patients incubated with LGG conditioned media (CM) showed reduced expression of TNFα and IL-17 compared with the corresponding expression in controls (P < 0.05). Incubation with a double dose of LGG increased mucosal adhesion and the anti-inflammatory effects (P < 0.05). In the in vivo experiment, LGG was detectable only in the colon of patients who consumed the LGG formulation, and bowel cleansing did not affect LGG adhesion. UC patients who consumed the double LGG dose had increased mucosal concentrations of the bacteria and reduced TNFα and IL-17 expression compared with patients who consumed the regular dose (48% and 40% reduction, respectively, P < 0.05).CONCLUSION In an ex vivo organ culture model, LGG showed consistent adhesion and anti-inflammatory effects. Colonization by LGG after consumption for a week was demonstrated in vivo in the human colon. Increasing the administered dose increased the adhesion and effectiveness of the bacteria. For the first time, we demonstrated that LGG effectively adheres to the colonic mucosa and exerts antiinflammatory effects, both ex vivo and in vivo. | Cristiano Pagnini Vito Domenico Corleto Michela Martorelli Claudio Lanini Giancarlo D'Ambra Emilio Di Giulio Gianfranco Delle Fave | 2018 | World Journal of Gastroenterology2018，24，41： | 2 |
| 2 | Digenea parasites of jellyfish and ctenophores of the southern Atlantic显示文摘 | Martorelli S R | 2001 | Hydrobiologia2001，451，： | 1 |
| 3 | Epidemiological and clinical study of viral respiratory tract infections in children from Italy显示文摘 | Fabbiani M Terrosi C Martorelli B | 2009 | J Med Virol2009，81，4： | 1 |
| 4 | Effects of shortterm isokinetic training with reciprocal knee extensors agonist and antagonist muscle actions:a controlled and randomized trial显示文摘 | Cunha R Carregaro RL Martorelli A | 2013 | Braz J Phys Ther2013，17，2： | 1 |
| 5 | Epidemiological and clinical study of viral respiratory tract infections in children from Italy 显示文摘 | Fabbiani M Terrosi C Martorelli B Valentini M Bernini L Cellesi C Cusi MG | 2009 | J Med Vriol2009，81，4： | 1 |
| 6 | Positive effecton hematopoiesis in patients with myelodysplasticsydromereceiving de- ferasirox as oral iron chelation therapy: a briefreview 显示文摘 | Guafiqlia R Martorelli MC Villani O | 2011 | Leuk Res2011，35，5： | 1 |
| 7 | Retinoids as critical modulators of immune functions:new therapeutic perspectives for old compounds显示文摘 | Montrone M Martorelli D Rosato A | 2009 | Endocr Metab Immune Disord Drug Targets2009，9，2： | 1 |
| 8 | Positive effects onhematopoiesis in patients with myelodysplastic syndrome receivingdeferasirox as oral iron chelation therapy : A brief review显示文摘 | Guariglia R Martorelli MC Villani 0 | 2011 | LeukRes2011，35，5： | 1 |
| 9 | Epidemiological and clinical study of viral respiratory tract infections in children from ltaly显示文摘 | Fabbiani M Terrosi C Martorelli B etal | 2009 | J MedVriol2009，81，4： | 1 |
| 10 | Retinoids as criti- cal modulators of immune functions: new therapeutic per- spectives for old compounds显示文摘 | Montrone M Martorelli D Rosato A et a l | 2009 | Endocr Metab Immune Dis- ord Drug Targets2009，9，2： | 1 |
| 11 | Mechanical behavior of post-restored upper canine teeth:A 3D FE analysis显示文摘 | Ausiello P Franciosa P Martorelli M | 2011 | Dent Mater2011，27，12： | 1 |
| 12 | Mechanical behavior of post-restored upper canine teeth: A 3D FE analysis 显示文摘 | Ausiello P Franciosa P Martorelli M | 2011 | DentMater2011，27，12： | 1 |
| 13 | Exploiting the interplay between innate and adaptive immunity to improve immunotherapeutic strategies for epstein-Barr-virus-driven disorders显示文摘 | Martorelli D Muraro E Merlo A | 2012 | Clin Dev Immunol2012，2012，93： | 1 |
| 14 | Numerical fa- tigue 3D-FE modeling of indirect composite-restored posts- rior teeth 显示文摘 | Ausiello P Franciosa P Martorelli M | 2011 | Dent Mater2011，27，5： | 1 |
| 15 | A comparison between customized clear and removable orthodontic appli- ances manufactured using RP and CNC techniques显示文摘 | Martorelli M Gerbino S Giudice M | 2012 | Dent Mater2012，29，2： | 1 |
| 16 | Spontaneous T cell responses to Epstein-Barr virus-encoded BARF1 protein and derived peptides in patients with nasopharyngeal carcinoma:bases for improved immunotherapy显示文摘 | Martorelli D Houali K Caggiari L | 2008 | Int J Cancer2008，123，5： | 1 |
| 17 | Vascular endothelial growth factor monitoring in advanced hepatocellularcarcinoma patients treated with radiofrequency ablation plus octreotide: A singlecenter experience显示文摘 | L. Montella R. Addeo M. Caraglia V. Faiola R. Guarrasi B. Vincenzi A. Palmeri E. Capasso V. Nocera L. Tarantino M. Ariete A. Martorelli S. Del Prete | 2008 | Oncology Reports2008，，2： | 1 |
| 18 | Therapy with adjunctive doxycycline local delivery in patients with type 1 diabetes mellitus and periodontitis显示文摘 | Martorelli de Lima AF Cury CC Palioto DB | 2004 | J Clin Periodontol2004，31，8： | 1 |
| 19 | Epidemiological and clinical study of viral respiratory tract infections in children from Italy显示文摘 | Fabbiani M Terrosi C Martorelli B | | 0，，4： | 1 |
| 20 | Retinoids as critical modulators of immune functions: new therapeutic perspectives for old compounds显示文摘 | Montrone M Martorelli D Rosato A | 2009 | Endocr Metab Immune Disord Drug Targets2009，9，2： | 1 |
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