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| 1 | MicroRNAs as a potential prognostic factor in gastric cancer显示文摘AIM:To compare the microRNA (miR) profiles in the primary tumor of patients with recurrent and non-recurrent gastric cancer.METHODS:The study group included 45 patients who underwent curative gastrectomies from 1995 to 2005 without adjuvant or neoadjuvant therapy and for whom adequate tumor content was available.Total RNA was extracted from formalin-fixed paraffin-embedded tumor samples,preserving the small RNA fraction.Initial profiling using miR microarrays was performed to identify potential biomarkers of recurrence after resection.The expression of the differential miRs was later verified by quantitative real-time polymerase chain reaction (qRT-PCR).Findings were compared between patients who had a recurrence within 36 mo of surgery (bad-prognosis group,n=14,31%) and those who did not (good-prognosis group,n=31,69%).RESULTS:Three miRs,miR-451,miR-199a-3p and miR-195 were found to be differentially expressed in tumors from patients with good prognosis vs patients with bad prognosis (P<0.0002,0.0027 and 0.0046 respectively).High expression of each miR was associated with poorer prognosis for both recurrence and survival.Using miR-451,the positive predictive value for non-recurrence was 100% (13/13).The expression of the differential miRs was verified by qRT-PCR,showing high correlation to the microarray data and similar separation into prognosis groups.CONCLUSION:This study identified three miRs,miR-451,miR-199a-3p and miR-195 to be predictive of recurrence of gastric cancer.Of these,miR-451 had the strongest prognostic impact. | Baruch Brenner Moshe B Hoshen Ofer Purim Miriam Ben David Karin Ashkenazi Gideon Marshak Yulia Kundel Ronen Brenner Sara Morgenstern Marisa Halpern Nitzan Rosenfeld Ayelet Chajut Yaron Niv Michal Kushnir | 2011 | World Journal of Gastroenterology2011，17，35： | 31 |
| 2 | The matrix metalloproteinase stromelysin-3 cleaves laminin receptor at two distinct sites between the transmembrane domain and laminin binding sequence within the extracellular domain显示文摘The matrix metalloproteinase (MMP) stromelysin-3 (ST3) has long been implicated to play an important role in extracellular matrix (ECM) remodeling and cell fate determination during normal and pathological processes. However like other MMPs, the molecular basis of ST3 function in vivo remains unclear due to the lack of information on its physiological substrates. Furthermore, ST3 has only weak activities toward all tested ECM proteins. Using thyroid hormone-dependent Xenopus laevis metamorphosis as a model, we demonstrated previously that ST3 is important for apoptosis and tissue morphogenesis during intestinal remodeling. Here, we used yeast two-hybrid screen with mRNAs from metamorphosing tadpoles to identify potential substrate of ST3 during development. We thus isolated the 37 kd laminin receptor precursor (LR). We showed that LR binds to ST3 in vitro and can be cleaved by ST3 at two sites distinct from where other MMPs cleave. Through peptide sequencing, we determined that the two cleavage sites are in the extracellular domain between the transmembrane domain and laminin binding sequence. Furthermore, we demon strated that these cleavage sites are conserved in human LR. These results together with high levels of human LR and ST3 expression in carcinomas suggest that LR is a likely in vivo substrate of ST3 and that its cleavage by ST3 may alter cell-extracellular matrix interaction, thus, playing a role in mediating the effects of ST3 on cell fate and behavior ob- served during development and pathogenesis. | Tosikazu AMANO Olivia KWAK Anastasia MARSHAK | 2005 | Cell Research2005，15，3： | 5 |
| 3 | Gold nanoparticles disturb nuclear chromatin decondensation in mouse sperm in vitro显示文摘 | Zakhidov S T Marshak T L Malolina E A | 2010 | Biochemistry Supplement2010，4，34： | 1 |
| 4 | Effect of gold nanoparticles on mouse spermatogenesis显示文摘 | Zakhidov S T Pavliuchenkova S M Marshak T L | 2012 | Izvestiia Akademii Nauk Seriia Biologicheskaia2012，3，27： | 1 |
| 5 | Beta-Amyloid regulates gene expression of glial trophic substance S100 beta in C6 glioma and primary astrocyte cultures 显示文摘 | Pena LA Brecher CW Marshak DR | 1995 | Brain Res Mol Brain Res1995，34，1： | 1 |
| 6 | Ramsay Hunt syndrome: a cranial polyneuropathy 显示文摘 | Aviel A Marshak G | 1982 | Am J Otolaryngol1982，3，1： | 1 |
| 7 | CALIPSO observations of transatlantic dust: vertical stratification and effect of clouds显示文摘 | Yang W. Marshak A. Várnai T. Kalashnikova O. V. Kostinski A. B | 2012 | Atmospheric Chemistry and Physics2012，，291： | 1 |
| 8 | Prognostic factors for local control of early glottic cancer : the Rabin Medical Center retrospective study on 207 patients 显示文摘 | Marshak G Brenner B Shvero J | 1999 | Int J Radiat Oncol Binl Phys1999，43，5： | 1 |
| 9 | Functional conservation of regulatory elements in the pdx-1 gene 显示文摘 | Marshak S Bantustan E Shoshkes M | 2000 | Mol Cell Biol2000，20，： | 1 |
| 10 | Synthetic peptide substrates for casein kinase Ⅱ 显示文摘 | Marshak D R Carroll D | 1991 | Methods Enzymol1991，200，： | 1 |
| 11 | Photoelastic analysis of stress transfer by endodontically treated teeth to the supporting structure using different restorative techniques显示文摘 | Assif D Oren E Marshak B L | 1989 | The Journal of prosthetic dentistry1989，61，5： | 1 |
| 12 | Partner communication and HIV testing among US Chinese college students 显示文摘 | Trieu SL Modeste NN Marshak HH | 2010 | Am J Health Behav2010，34，3： | 1 |
| 13 | Restoring teeth following crown lengthening procedures显示文摘 | Assif D Pilo R'Marshak B | 1991 | Pmsthct Dent1991，65，1： | 1 |
| 14 | Multilineage potential of adult human mesenchymal stem cells 显示文摘 | Pittenger MF Mackay AM Beck SC Jaiswal RK Douglas R Mosca JD Moorman MA Simonetti DW Craig S Marshak DR | 1999 | Science1999，284，： | 1 |
| 15 | Gold nanoparticles disturb nuclear chromatin decondensation in mouse sperm in vitro显示文摘 | Zakhidov S T Marshak T L Malolina E A | 2010 | Biologicheskie Membrany2010，27，4： | 1 |
| 16 | Note on the spherical harmonic method as applied to the milne problem for a sphere 显示文摘 | Marshak R E | 1947 | Phys Rev1947，71，： | 1 |
| 17 | A metal-free organic-inorganic aqueous flow battery显示文摘 | Huskinson Brian Marshak Michael P Suh Changwon Er Süleyman Gerhardt Michael R Galvin Cooper J Chen Xudong Aspuru-Guzik Alán Gordon Roy G Aziz Michael J | 2014 | Nature2014，505，7482： | 1 |
| 18 | Organizational Discourse As Situated Symbolic Action: Application Through an Od Inter- vention 显示文摘 | Hearcleous L Marshak R J | 2004 | Academy of Marmgeament Proceedings2004，1，： | 1 |
| 19 | Brine migration across north America-the plate tectonics of groundwater 显示文摘 | Bethke C M Marshak S | 1990 | Annual Review of Earth and Planetary Sciences1990，18，： | 1 |
| 20 | Structural and functional properties of calmodulin from the eukaryotic micro- organism dictyostelium discoideum 显示文摘 | Marshak DR Clark M Roberts DM Watterson DM | 1984 | Biochemistry1984，23，13： | 1 |
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