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| 1 | Burden of diabetic foot ulcer in Nigeria: Current evidence from the multicenter evaluation of diabetic foot ulcer in Nigeria显示文摘BACKGROUND Nigeria bears the greatest burden of diabetes prevalence in Sub-Saharan Africa. Diabetic foot ulcer (DFU) is a serious and potentially life-threatening complication of diabetes. Significant improvements in diabetic foot incidence and outcomes have been recorded in many Western countries in the past decade. However, the current burden of DFU in Nigeria is largely unknown. AIM To evaluate the patients’ profile, ulcer characteristics, associated co-morbidities and outcome of patients with DFU in Nigeria. METHODS Multicenter evaluation of diabetic foot ulcer in Nigeria was a one year multicenter observational study of patients hospitalized for DFU in six tertiary health institutions in Nigeria from March 2016 to March 2017. Demographic and diabetes information, ulcer characteristics and associated co-morbidities were assessed. Relevant laboratory and imaging studies were performed. All patients received appropriate multi-disciplinary care and were followed up until discharge or death. Outcome variables of interest were ulcer healing, lower extremity amputation (LEA), duration of hospitalization and mortality. RESULTS A total of 336 patients (55.1% male) with mean age of 55.9 ± 12.5 years were enrolled into this study. Majority (96.1%) had type 2 diabetes. Only 25.9% of the subjects had prior foot care knowledge. Most of the subjects presented late to the hospital and median (IQR) duration of ulcer at presentation was 39 (28-54) d. Ulcers were already advanced (Wagner grades ≥ 3) in 79.2% of the subjects while 76.8% of the ulcers were infected at the time of admission. The commonest comorbidities were systemic hypertension, anemia and hyperglycemic emergencies. One hundred and nineteen subjects (35.4%) suffered LEA while 10.4% left against medical advice. The median (IQR) duration of hospitalization was 52.0 (29-66) d with case fatality rate of 20.5%. CONCLUSION The burden of DFU in Nigeria is very high. The major gaps include low level of foot care knowledge among diabetic patients, overdependence on self-medication and unorthodox medicine following development of foot ulceration, late hospital presentation, and high amputation and mortality rates. Extensive foot care education within the framework of a multi-disciplinary foot care team is highly desirable. | Ejiofor Ugwu Olufunmilayo Adeleye Ibrahim Gezawa Innocent Okpe Marcelina Enamino Ignatius Ezeani | 2019 | World Journal of Diabetes2019，10，3： | 5 |
| 2 | The N363S and I559N single nucleotide polymorphisms of the h-GR/NR3C1gene in patients with bronchial asthma显示文摘 | Micha? Panek Tadeusz Pietras Adam Antczak Artur Fabijan Marcelina Przem?cka Pawe? Górski Piotr Kuna Janusz Szemraj | 2012 | International Journal of Molecular Medicine2012，，1： | 2 |
| 3 | Therapy of type 1 diabetes with CD4 + CD25 high CD127-regulatory T cells prolongs survival of pancreatic islets — Results of one year follow-up显示文摘 | Natalia Marek-Trzonkowska Ma?gorzata My?liwiec Anita Dobyszuk Marcelina Grabowska Ilona Derkowska Jolanta Ju?cińska Rados?aw Owczuk Agnieszka Szadkowska Piotr Witkowski Wojciech M?ynarski Przemys?awa Jarosz-Chobot Artur Bossowski Janusz Siebert Piotr Trzo | 2014 | Clinical Immunology2014，，1： | 1 |
| 4 | Stochastic blind equalization based on PDF fitting using Parzen estimator显示文摘 | MARCELINA L SANTAMARIA I ERDOGMUS D | 2005 | IEEE Trans on Signal Prcess2005，53，2： | 1 |
| 5 | 酪醇通过提高糖尿病高糖环境下骨骼肌细胞的活力和旁分泌功能促进糖尿病小鼠缺血下肢的血管新生显示文摘目的下肢缺血性疾病(HLI)是糖尿病的主要并发症之一,治疗的关键是体内血管重构。但由于糖尿病伴随持续的高血糖,机体发生系统性损伤,血管新生能力被严重破坏,所以阻碍了治疗性血管新生促糖尿病血管重构的作用。骨骼肌细胞(SMC)分泌血管新生因子,通过细胞间交流在血管重构中发挥重要作用。但在HLI的糖尿病病理条件下(高糖、低氧),骨骼肌细胞功能受损,导致SMC的促血管新生作用下降。小分子化合物酪醇(TYR)具有细胞保护和抗氧化的作用,但在高糖环境中,其对SMC的保护和促血管新生作用仍是未知的。方法将骨骼肌细胞分为3组:对照、高糖、高糖+TYR。利用MTIT法、细胞内活性氧(ROS)检测法和流式细胞术细胞凋亡检测法等考察TYR对SMC细胞活力和凋亡的影响;利用Western Blotting和ELISA考察TYR对血管新生因子(VEGF-A和PDGF-BB)表达和分泌的作用;EdU和transwell实验观察SMC细胞分泌物对血管内皮细胞(HUVECs)和血管平滑肌细胞(MOVAS)增殖和迁移的影响;体内实验通过构建糖尿病下肢缺血小鼠模型,肌肉注射TYR,应用激光多普勒扫描仪分析糖尿病下肢缺血小鼠下肢血流恢复情况,考察酪醇对糖尿病下肢缺血小鼠的治疗作用。结果(1)TYR抑制了高血糖诱导的细胞内活性氧(ROS)水平,降低了细胞凋亡率,提高了SMC细胞活力;(2)TYR促进了高糖环境中SMC细胞VEGF-A和PDGF-BB的表达和分泌;(3)TYR预处理SMC后收集到的条件培养基促进了HUVECs和MOVAS的增值和迁移;(4)TYR改善了糖尿病下肢缺血模型小鼠的血管新生和血流恢复功能。结论用TYR处理SMC可显著提高骨骼肌的细胞活力和旁分泌功能,促进缺血组织中血流恢复和血管新生。研究结果提示对于糖尿病下肢缺血疾病,酪醇是一种具有潜在应用价值的促进血管新生作用的小分子化合物。 | 张建琪 Olivia Marcelina Dyah Ari Nugrahaningrum 徐志玲 刘才平 王贵学 吴寿荣 江启慧 | 2019 | 医用生物力学2019，34，A01： | 0 |
| 6 | Salidroside Pretreatment to Mesenchymal Stem Cells Improves Cell Survival and Migration to Promote Diabetic Wound Healing显示文摘Objective Diabetic patients pose a greater challenge in managing chronic wound healing,leading to a higher amputation risk compared to non-diabetic patients.Due to their paracrine function by secreting various cytokines and angiogenic factors,mesenchymal stem cells(MSCs)have been acknowledged to be a potential agent in modulating wound healing process.However,post-transplanted MSCs are vulnerable to death,indicating poor survival and migration ability in the wound site of the host,especially under hyperglycemia.As hyperglycemia induces reactive oxygen species(ROS)generation and cellular apoptosis,improvement of MSCs survival and migration potentials under hyperglycemia could contribute to a more efficient MSCs-based wound healing therapy.Salidroside(Sa),a small-molecule drug derived from Rhodiola plant,has been proved to enhance the paracrine function of skeletal muscle cells,as well as their migration even under hypoxichyperglycemia.Herein,we investigated whether Sa could improve the survival and migration potentials of MSCs,subsequently enhance the wound healing process under hyperglycemia.Methods MSCs were cultured under three conditions:low glucose,high glucose,and high glucose+Sa.qPCR analysis and western blotting were done to examine the mRNA and protein expression level of several factors which are important in upregulating the wound healing process.MTT colorimetric assay,intracellular ROS detection,and flow cytometry assay were employed to examine the effect of Sa in MSCs survival.Transwell chamber assay,scratch assay,and phalloidin staining were done to elucidate the role of Sa in regulating MSCs migration potential.For in vivo experiment,diabetic wound healing mice model was generated to elucidate the effect of Sa-pretreated MSCs transplantation in wound closure rate,as well as re-epithelization status,observed with hematoxylin and eosin staining.The diabetic wound healing mice model were divided into three groups:1)mice injected with PBS,2)mice transplanted with PBS-pretreated MSCs,and 3)mice transplanted with Sa-pretreated MSCs.Results(1)Hyperglycemic condition induced the generation of ROS and suppressed total cell number of MSCs,while Sa treatment into MSCs restored these hyperglycemia-induced alterations.In line with this,total apoptotic cells were also suppressed by treating MSCs with Sa.The expression level of cell survival factor,heme-oxygenase 1(HO-1),was enhanced in Sa-pretreated MSCs.Further treatment of HO-1 inhibitor into Sa-pretreated MSCs nullified the ROS level and total apoptotic cells,indica-ting the importance of HO-1 in mediating the Sa-induced survival of MSCs under hyperglycemia.(2)Transwell chamber and scratch assay results showed that Sa-pretreated MSCs have a higher migration potential under hyperglycemia,supported by higher F-actin polymerization fractal dimension.Fibroblast growth factor 2(FGF2)and hepatocyte growth factor(HGF)expression level,which are essential factors for cell migration,were also improved in Sa-pretreated MSCs under hyperglycemia.(3)In diabetic wound healing mice model,transplantation of Sa-pretreated MSCs resulted in significantly improved wound closure rate and re-epithelization.The protein levels of HO-1,FGF2,and HGF were also enhanced in the tissues obtained from the wound site of diabetic wound healing mice model which were transplanted with Sa-pretreated MSCs.Conclusions Salidroside pretreatment on MSCs could improve their survival and migration potentials,subsequently promoting wound healing process under hyperglycemia.This prospective MSC-based therapy could serve as a novel strategy to improve diabetic wound healing. | Olivia Marcelina Jianqi Zhang Dyah Ari Nugrahaningrum Zhiling Xu Guixue Wang Li Yang Shourong Wu Vivi Kasim | 2019 | 医用生物力学2019，34，A01： | 0 |
| 7 | 海外3G领先一步显示文摘海外国家的3G已经开始广泛应用数年,并掌握了一定的3G运营经验了。美国的技术创新、口本周到细致的服务、法国政策的支持等都为本国的3G发展奠定坚实的基础,引领了世界3G潮流。 | Marcelina | 2009 | 创意世界2009，，2： | 0 |
| 8 | Effect of nanosilver(nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions显示文摘Cereals, including barley, have been playing a key role in human diet for a long time. The objective of the present study was to determine the effect of nanosilver(nAg) on limitation of infections, morphological features, and their chemical composition of young barley seedlings under in vitro condition. Addition of 0, 2, 4, 6 and 8 mg dm^–3 nAg into MS medium was used.Obtained results showed that the effect on the morphological features depended on the nAg concentration. The addition of 6 and 8 mg dm^–3 nAg into MS medium limited the number of infected barley embryos in vitro, whereas 4 and 8 mg dm^–3 nAg resulted in the highest seedlings with the longest roots. nAg in the medium affected the colour of leaves and increased the contents of chlorophyll and β-carotene, in particular in seedlings growing in MS medium supplied with 6 mg dm^–3 nAg.The addition of 8 mg dm^–3 had the greatest effect on the contents of vitamin C and E in young barley seedlings. It was found that the contents of K and Ca in the young barley leaves were lower, as compared to control plants. The presence of 6 mg dm^–3 nAg in the medium resulted in an increased contents of N, Mg, Zn, Cu, and P. Hence, a diversified effect of nAg on individual groups of polyphenolic compounds was noticed. The presence of 2 and 8 mg dm^–3 nAg caused higher content values of polyphenolic compounds in young barley leaves. | Marcelina Krupa-Malkiewicz Jan Oszmiański Sabina Lachowicz Malgorzata Szczepanek Boguslawa Jalkiewicz Kamila Pachnowska Ireneusz Ochmian | 2019 | Journal of Integrative Agriculture2019，18，8： | 0 |
| 9 | Environment and Age of the Upper Devonian‒Carboniferous Zhulumute and Hongguleleng Formations,Junggar Basin,NW China:Ichnological and Palynological Aspects显示文摘Two Upper Devonian to Lower Carboniferous sections,namely the Bulongguoer and Haer(Gennaren area)in the Junggar Basin,Central Asian Orogenic Belt,in northwestern China were examined with regard to ichnology and palynology.The deposits of the Hongguleleng Formation and Namu Member of the Heishantou Formation,respectively,are mostly unbioturbated or poorly bioturbated,with poorly diversified trace fossils,which indicate unfavourable conditions for the burrowing benthic community during the Late Devonian and Early Carboniferous.The main factor governing the growth of the bioturbating population was the rapid sedimentation of fine-grained,volcanic material documented as tempestites,which buried the benthic fauna.In the Bulongguoer section,the presence of miospores Teichertospora torquata and Grandispora gracilis enabled identification of the broad Teichertospora torquata–Grandispora gracilis Miospore Zone from the transitional Frasnian/Famennian interval.More accurate identification of palynostratigraphical levels(DE and CZ Miospore zones)is only tentative.On the basis of palynofacies analysis,samples from the lower Bulongguoer section indicate deposition in more proximal conditions,whereas samples from the top of the section indicate deposition in a more distal environment.In the Haer section,only a general biostratigraphic indication for the Tournaisian was possible,owing to poor preservation of organic matter. | MichałS TACHACZ Marcelina KONDAS PawełFILIPIAK MA Xueping | 2021 | Acta Geologica Sinica(English Edition)2021，95，3： | 0 |
| 10 | 阿梅格尔巴克/哥本山显示文摘垃圾焚烧发电厂阿梅格尔巴克/哥本山位于工业区,多年来,该工业区已成为冒险者们的极限运动目的地。原始的工业设施为各种极限运动活动提供了场所,如缆绳滑水、卡丁车和攀岩等。阿梅格尔巴克/哥本山是该地区最重要的地标,其建筑亟须更新。我们提出了一种新型的垃圾焚烧发电厂,这种工厂能产生经济、环保和社会效益。 | Nanna Gyldholm M■ller Alberto Cumerlato Aleksander Wadas Alexander Codda Alexander Ejsing Alexandra Gustafsson Alina Tamosiunaite Armor Gutierrez Anders Hjortn■s Andreas Klok Pedersen Annette Jensen Ariel Wallner Ask Andersen Balaj IIulian Blake Smith Borko Nikolic Brygida Zawadzka Buster Christensen Chris Falla Chris Zhongtian Yuan Daniel Selensky Dennis Rasmussen Espen Vik Finn N■rkj■r Franck Fdida Gonzalo Castro Gül Ertekin George Abraham Helen Chen Henrick Poulsen Henrik R■mer Kania Horia Spirescu Jakob Ohm Laursen Jelena Vucic Jeppe Ecklon Ji-young Yoon Jing Xu Joanna Jakubowska Johanna Nenander Kamilla Heskje Katarzyna Siedlecka Krzysztof Marciszewski Laura W■tte Liang Wang Lise Jessen Long Zuo Maciej Zawadzki Mads Enggaard Stidsen Marcelina Kolasinska Marcos Bano Maren Allen Mathias Bank Matti N■rgaard Michael Andersen Narisara Ladawal Schr■der Niklas A.Rasch Nynne Madsen ■ssur Nols■ Pero Vukovic Richard Howis Ryohei Koike Se Hyeon Kim Simon Masson Sunming Lee Toni Mateu Xing Xiong Zoltan David Kalaszi Rasmus Hjortshoj 陈子钰(译) | 2019 | 城市环境设计2019，0，2： | 0 |
| 11 | 瑞士巴塞尔老工业混凝土仓房改建显示文摘巴塞尔的Transitlager坐落于Dreispitz的未来城区中心位置,是众多用于美术馆、餐厅和创意产业的新老建筑中的其中一座。它位于设想的Soho区和皇后区的边缘。与城市一样,周围的工业区域也充斥着基础设施的几何形体。通过对原仓库进行新的功能规划和扩建,使其成为一座多功能的建筑. | Agnete Jukneviciute Alexandra Gustaffson Andreas Johansen Annette Jensen Barbara Srpkova Buster Christensen Camila Stadler Dennis Rasmussen Dominic Black Enea Michelesio Erik de Haan Gül Ertekin Franck Fdida Helen Chen Ioannis Gio Jan Magasanik Jesper Andersen Lorenzo Boddi Marcelina Kolasinska Teresa Fernandez Martin Voelkle Miao Zhang Michael Sch■nemann Mikkel Marcker Stubgaard Ole Elkj■r-Larsen Ricardo Palma Ryohei Koike Sergiu Calacean Tobias Hjortdal Laurian Ghinitoiu 朱翰林(译) | 2019 | 城市环境设计2019，0，2： | 0 |
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