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| 1 | 小反刍兽疫病毒(PPRV)N蛋白的表达与ELISA检测方法的建立显示文摘通过对下载的16株小反刍兽疫病毒(PPRV)N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的PPRV N蛋白。用PPRV N蛋白制备免疫血清,经国际标准血清标化后作为参考阳性血清,同时以PPRV N蛋白为抗原建立间接ELISA(I-ELISA)方法。对山东省的467份羊血清检测,使用S/P(样品值/阳性值)平均值加3倍标准差的方法,计算出其临界点为0.28。应用间接ELISA和针对H蛋白的单抗为基础的C-ELISA检测来自于疫区的69份血清,结果两者的符合率为79.7%。 | 张喜悦 王志亮 刘春菊 李林 包静月 何昭阳 Mandy C Carrie B Anderson J | 2008 | 中国兽医学报2008，28，2： | 8 |
| 2 | Encapsulation of Curcumin in Cationic Micelles Suppresses Alkaline Hydrol- ysis 显示文摘 | MANDY H M L HANNAH C TAK W K | 2008 | Langmuir2008，24，： | 1 |
| 3 | Use of streamflow data to estimate base flow/ground-water recharge for Wisconsin显示文摘 | Warren A G Mandy J R Ellen J C | 2007 | Journal of the American Water Resources Association2007，43，1： | 1 |
| 4 | Acute respiratory distress syndrome of the contralateral lung after reexpansion pulmonary edema of a collapsed lung显示文摘 | Her C Mandy S | | 0，，04： | 1 |
| 5 | Undergraduate students ' attitudes to communication skills learning differ depending on year of study and gender显示文摘 | Jennifer C Karen F & Mandy M | 2005 | Medical Teacher2005，27，3： | 1 |
| 6 | Amino acid residues in the N-terminal region of the PA subunit of influenza A virus RNA polymerase play a critical role in protein stability, endonuclease J activity, cap binding, and virion RNA promoter binding 显示文摘 | Koyu H Florian I S Mandy C | 2006 | J Virol2006，80，16： | 1 |
| 7 | Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence显示文摘 | Giovanni M C Mandy J C Robert J H | 2002 | Molecular Breeding2002，9，4： | 1 |
| 8 | Fauk geometries in base- ment-induced wrench faulting under different initial stress states 显示文摘 | Taylor M A Mandy G Sijpesteijn C H K | 1986 | Journal of Structural Geology1986，8，7： | 1 |
| 9 | Social communication competence and functional adaptation in a general population of children: preliminary evidence for sex-by-verbal IQ differential risk显示文摘 | Skuse DH Mandy W Steer C | 2009 | J Am Acad Child Adolesc Psychiatry2009，48，2： | 1 |
| 10 | Macular thickness and volume parameters measured using optical coherence tomography (OCT) for evaluation of glaucoma patients显示文摘 | Barisi(c) F Sicaja A J Ravli(c)MM Novak-Laus K Ivekovi(c)R Mandi(c) Z | | 0，，02： | 1 |
| 11 | Recommendations for tuberculosis screening before and during treatment with tumour necrosis factor inhibitors显示文摘 | Mandi(c) D Curci(c) R Radosavljevi(c) G | | 0，，3： | 1 |
| 12 | Seemingly unrelated regressions under additive het?eroscedasticity: Theory and share equation applications显示文摘 | Mandy D M Martins-Filho C | 1993 | J Econom1993，58，： | 1 |
| 13 | Research article: exten- sions to least-cost path algorithms for roadway planning 显示文摘 | Yu C Q Lee J Mandy J | 2003 | International Journal of Geographical Information Science2003，17，4： | 1 |
| 14 | Assessment of a customized immobilization system for head and neck IMRT using electronic portal imaging显示文摘 | Mandy H M Urbano T G Mubata C | 2005 | Radiother Oncol2005，77，： | 1 |
| 15 | Acute respiratory distress syndrome of the contralateral lung after reexpansion pulmonary edema of a collapsed lung显示文摘 | Her C Mandy S | 2004 | J Clin Anesth2004，16，4： | 1 |
| 16 | Changing self - efficacy fol- lowing first stroke : preliminary study of a novel self - man- agement intervention 显示文摘 | Jones F Mandy A Partridge C | 2009 | Clin Rehabil2009，23，6： | 1 |
| 17 | Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence显示文摘 | Giovanni M C Mandy J C Robert J H | 2002 | Molecular Breeding2002，9，： | 1 |
| 18 | Polyaniline as Cathodic Material for Electrochemical Energy Sources:The Role of Morphology显示文摘 | Mandi c Z Rokovi c M K Pokupcic T | 2009 | Electrochimica Acta2009，54，10： | 1 |
| 19 | Identification of micmsatellite markers for fragrance in rice by analysis of the rice genome sequence显示文摘 | GIOVANNI M C MANDY J C ROBERT J H | 2002 | Molecular Breeding2002，9，4： | 1 |
| 20 | Results in the determination of some Hosta varieties by the method of isoelectric focusing 显示文摘 | Mandy A Stefanovits Banyai E Imre C | 2001 | International Journal of Horticultural Science2001，7，1： | 1 |
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