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| 1 | Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes显示文摘BACKGROUND End-stage liver disease is a global health complication with high prevalence and limited treatment options.Cell-based therapies using mesenchymal stem cells(MSCs)emerged as an alternative approach to support hepatic regeneration.In vitro preconditioning strategies have been employed to strengthen the regenerative and differentiation potential of MSCs towards hepatic lineage.Chemical compounds of the triterpene class;glycyrrhizic acid(GA)and 18β-glycyrrhetinic acid(GT)possess diverse therapeutic properties including hepatoprotection and anti-fibrosis characteristics.They are capable of modulating several signaling pathways that are crucial in hepatic regeneration.Preconditioning with hepato-protective triterpenes may stimulate MSC fate transition towards hepatocytes.AIM To explore the effect of GA and GT on hepatic differentiation of human umbilical cord-MSCs(hUC-MSCs).METHODS hUC-MSCs were isolated and characterized phenotypically by flow cytometry and immunocytochemistry for the expression of MSC-associated surface molecules.Isolated cells were treated with GA,GT,and their combination for 24 h and then analyzed at three time points;day 7,14,and 21.qRT-PCR was performed for the expression of hepatic genes.Expression of hepatic proteins was analyzed by immunocytochemistry at day 21.Periodic acid Schiff staining was performed to determine the functional ability of treated cells.RESULTS The fusiform-shaped morphology of MSCs in the treatment groups in comparison with the untreated control,eventually progressed towards the polygonal morphology of hepatocytes with the passage of time.The temporal transcriptional profile of preconditioned MSCs displayed significant expression of hepatic genes with increasing time of differentiation.Preconditioned cells showed positive expression of hepatocyte-specific proteins.The results were further corroborated by positive periodic acid Schiff staining,indicating the presence of glycogen in their cytoplasm.Moreover,bi-nucleated cells,which is the typical feature of hepatocytes,were also seen in the preconditioned cells.CONCLUSION Preconditioning with glycyrrhizic acid,18β-glycyrrhetinic acid and their combination,successfully differentiates hUC-MSCs into hepatic-like cells.These MSCs may serve as a better therapeutic option for degenerative liver diseases in future. | Abiha Fatima Tuba Shakil Malick Irfan Khan Aisha Ishaque Asmat Salim | 2021 | World Journal of Stem Cells2021，13，10： | 3 |
| 2 | Experimental therapy of human glioma by means of a genetically engineered virus mutant显示文摘 | Martuza R L Malick A Markert J M | 1991 | Science1991，252，5007： | 1 |
| 3 | Clinical applications of argon plasma coagulation in endos-copy显示文摘 | Malick KJ | 2006 | Gastroenterol Nurs2006，29，5： | 1 |
| 4 | Stock effects and seasonality in the FCOJ futures basis显示文摘 | Malick W M Ward R W | 1987 | The Journal of Futures Markets1987，，： | 1 |
| 5 | Microbial removal of organic sulfur from crude oil and the environment:Some new perspectives显示文摘 | MALICK K A | 1978 | Process Biochemistry1978，13，： | 1 |
| 6 | Coexpression of Notchl and NF-kappaB signaling pathway components in human cervical progression 显示文摘 | Ramdass B Malickal TT Lakshmi S ct al | 2007 | Gynecol 0ncol2007，104，2： | 1 |
| 7 | Clinical applications of argon plasma coagu- lation in endoscopy显示文摘 | Malick KJ | 2006 | Gastroenterol Nuts2006，29，5： | 1 |
| 8 | Distinct response to copper stress in halophyte Mesembryanthemum crystallinum显示文摘 | Thomas J C Malick F K Endreszl C | 1998 | Physiol Plant1998，102，： | 1 |
| 9 | Is evidence-based medicine teaching and learning directed at improving practice? 显示文摘 | Malick SM Hadley J Davis J | 2010 | J R SocMed2010，103，6： | 1 |
| 10 | Experimental therapy of human glioma by means of a genetically engineered virus mutant 显示文摘 | Martuza RL Malick A Markert JM ct al | 1991 | Science1991，252，5007： | 1 |
| 11 | Potentiation of yohimbine-induced lethality in mice:Predictor of antidepressant potential 显示文摘 | Malick JB | 1983 | Drug Dev Res1983，3，4： | 1 |
| 12 | Acoustic Techniques for Fatigue Cracking Mechanisms Characterization in Hot Mix Asphalt(HMA)显示文摘 | Malick Diakhaté Nicolas Larcher Mokhfi Takarli Nicolas Angellier and Christophe Petit | 2012 | International Conference on Cracking in Pavements2012，，： | 1 |
| 13 | Citric acid as a solid-state plasticizer for Eudragit RS PO 显示文摘 | Schilling S U Shah N H Malick A W | 2007 | Journal ofPharmacy and Pharmacology2007，59，： | 1 |
| 14 | Desvenlafaxine re- duces apoptosis in amygdala after myocardial infarction 显示文摘 | Malick M Gilbert K Barry M | 2014 | Brain Research Bulletin2014，109，： | 1 |
| 15 | Self emulsifying drug delivery systems (SEDDS) with polyglycolyzed glycerides for improving in vitro dissolution and oral absorption of lipophilic drugs 显示文摘 | Shah NH Carvajal MT Malick AW | 1994 | Int J Pharm1994，106，1： | 1 |
| 16 | A neurohistochemical blueprint for pain-induced loss of appetite显示文摘 | Malick A Jakubowski M Elmquist JK | 2001 | Pnas2001，98，17： | 1 |
| 17 | Vulnerability analysis and best practices for adapting IP telephony in critical infrastructure sectors显示文摘 | Cao F Saadat Malick | 2006 | IEEE Communications Magzine2006，44，4： | 1 |
| 18 | Bipolar radiofrequency compared with thermal balloon endometrial ablation in the office:a randomized controlled trial显示文摘 | Clark TJ Samuel N Malick S | 2011 | Obstet Gynecol2011，117，1： | 1 |
| 19 | Experimental therapy of human glioma by means of a genetically engineered virus mutant显示文摘 | Malick A Markert JM | 1991 | Science1991，252，： | 1 |
| 20 | Nonreflecting boundary condition in finite-element formulation for an elastic wave equation显示文摘 | Sarma G S Malick K Gadhinglakar V R | 1998 | Geophysics1998，63，3： | 1 |
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