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| 1 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 2 | Microencapsulated human mesenchymal stem cells decrease liver fibrosis in mice显示文摘 | Raphael P.H. Meier Redouan Mahou Philippe Morel Jeremy Meyer Elisa Montanari Yannick D. Muller Panayiotis Christofilopoulos Christine Wandrey Carmen Gonelle-Gispert Leo H. Bühler | 2014 | Journal of Hepatology2014，，： | 1 |
| 3 | Clinical expression of systemiclupus erythematosus with anti-U1-RNP and anti-Sm antibodies显示文摘 | LOPEZ-LONGO FJ GONZALEZ-FERNANDZE CM RO-DRIGUEZ MAHOU M | 1997 | Rev Clin Esp1997，197，： | 1 |
| 4 | Microencapsulated human mesenchymal stem cells decrease liver fibrosis in mice 显示文摘 | Meier RP Mahou R Morel P | 2015 | J Hepato12015，62，3： | 1 |
| 5 | Efficacy and safety of Etanercept, high - dose intravenous gammaglobulin and plasmapheresis combined therapy for lupus diffuse proliferative nephritis complicating pregnancy 显示文摘 | Micheloud D Nuno L Rodriguez- Mahou M | | Lupus0，15，12： | 1 |
| 6 | Microencapsulated hu- man mesenchymal stem cells decrease liver fibrosis in mice 显示文摘 | Meier RP Mahou R Morel P | 2015 | J Hepatol2015，62，3： | 1 |
| 7 | Label-free imaging of red blood cells and oxygenation with color third-order sum-frequency generation microscopy显示文摘Mapping red blood cells(RBCs)flow and oxygenation is of key importance for analyzing brain and tissue physiology.Current microscopy methods are limited either in sensitivity or in spatio-temporal resolution.In this work,we introduce a novel approach based on label-free third-order sum-frequency generation(TSFG)and third-harmonic generation(THG)contrasts.First,we propose a novel experimental scheme for color TSFG microscopy,which provides simultaneous measurements at several wavelengths encompassing the Soret absorption band of hemoglobin.We show that there is a strong three-photon(3P)resonance related to the Soret band of hemoglobin in THG and TSFG signals from zebrafish and human RBCs,and that this resonance is sensitive to RBC oxygenation state.We demonstrate that our color TSFG implementation enables specific detection of flowing RBCs in zebrafish embryos and is sensitive to RBC oxygenation dynamics with single-cell resolution and microsecond pixel times.Moreover,it can be implemented on a 3P microscope and provides label-free RBC-specific contrast at depths exceeding 600μm in live adult zebrafish brain.Our results establish a new multiphoton contrast extending the palette of deep-tissue microscopy. | Julia Ferrer Ortas Pierre Mahou Sophie Escoto Chiara Stringari Nicolas BDavid Laure Bally-Cuif Nicolas Dray Michel Negrerie Willy Supatto Emmanuel Beaurepaire | 2023 | Light(Science & Applications)2023，12，2： | 0 |
| 8 | Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy显示文摘A key property of the human cornea is to maintain its curvature and consequently its refraction capability despite daily changes in intraocular pressure.This is closely related to the multiscale structure of the corneal stroma,which consists of 1-3µm-thick stacked lamellae made of thin collagen fibrils.Nevertheless,the distribution,size,and orientation of these lamellae along the depth of the cornea are poorly characterized up to now.In this study,we use second harmonic generation(SHG)microscopy to visualize the collagen distribution over the full depth of 10 intact and unstained human corneas(500-600µm thick).We take advantage of the small coherence length in epi-detection to axially resolve the lamellae while maintaining the corneal physiological curvature.Moreover,as raw epi-detected SHG images are spatially homogenous because of the sub-wavelength size of stromal collagen fibrils,we use a polarimetric approach to measure the collagen orientation in every voxel.After a careful validation of this approach,we show that the collagen lamellae(i)are mostly oriented along the inferior-superior axis in the anterior stroma and along the nasal-temporal axis in the posterior stroma,with a gradual shift in between and(ii)exhibit more disorder in the anterior stroma.These results represent the first quantitative characterization of the lamellar structure of the human cornea continuously along its entire thickness with micrometric resolution.It also shows the unique potential of P-SHG microscopy for imaging of collagen distribution in thick dense tissues. | Clothilde Raoux Anatole Chessel Pierre Mahou Gaël Latour Marie-Claire Schanne-Klein | 2023 | Light(Science & Applications)2023，12，9： | 0 |
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