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| 1 | Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats显示文摘AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia(HEEP) leaves on gastric ulcer healing.METHODS In this rat study involving males, intact(cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity.RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males(52.44%), intact females(85.22%), and ovariectomized females(65.47%), confirming that HEEP accelerates the healing of acetic acidinduced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor(intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2(intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed.CONCLUSION In gastric ulcers, HEEP-induced healing(modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration. | Larissa Lucena Périco Vinícius Peixoto Rodrigues Rie Ohara Gabriela Bueno Vania Vasti Alfieri Nunes Raquel Cássia dos Santos Ana Carolina Lima Camargo Luis Antuio Justulin Joior Sérgio Faloni de Andrade Viviane Miranda Bispo Steimbach Luísa Mota da Silva Lúcia Regina Machado da Rocha Wagner Vilegas Catarina dos Santos Clélia Akiko Hiruma-Lima | 2018 | World Journal of Gastroenterology2018，24，38： | 3 |
| 2 | Extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum显示文摘We report a rare case of extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum.A 72-year-old Japanese man was admitted to our hospital presenting with a 3-mo history of epigastric pain, vomiting and weight loss.On computed tomography(CT) a wall thickening of the fourth part of the duodenum was observed.Multiple biopsies obtained from the lesion showed inflltration of plasma cells and lymphocytes, but they were not conclusive.The patient underwent resection of the lesion and, on histopathological examination, the lesion consisted of a dense and diffuse inflltrate of plasma cells and a few admixed lymphocytes with reactive follicles extending to the muscular propria.An extensive deposition of amyloid was also observed.Immunohistochemical stains revealed that a few plasmacytoid cells showed λ light chain staining, though most were κ light chain positive.These cells also were positive for CD138 and CD56 but negative for CD20 and CD79.The flndings were consistent with extramedullary plasmocytomaassociated with a massive deposit of amyloid in duodenum.A subsequent workup for multiple myeloma was completely negative.The patient showed no signs of local recurrence or dissemination of the disease after 12 mo follow-up.Because of the association of plasmocytoma and amyloidosis, the patient must be followed up because of the possible systemic involvement of the neoplasm and amyloidosis in future. | Fabiana Pirani Carneiro Maria de Nazareth Machado Sobreira Lívia Bravo Maia Alesso Cervantes Sartorelli Luiz Eduardo de Almeida Prado Franceschi Mauro Brito Brando Bárbara Wosnjuk Calaca Fernando Silva Lustosa Joo Vieira Lopes | 2009 | World Journal of Gastroenterology2009，15，28： | 2 |
| 3 | Probing the mechanism of the Heck reaction with arene diazonium salts by electrospray mass and tandem mass spectrometry 显示文摘 | SABINO A A MACHADO A H L ROQUE C D | 2004 | Angew Chem Int Ed2004，43，19： | 1 |
| 4 | A distribu- ted concurrency control mechanism for XML data 显示文摘 | Moreura L O SOUSA F R C MACHADO J C | 2011 | Journal of Compute and System Science2011，38，9： | 1 |
| 5 | Biocompatibility study for PVP wound dressing obtained in different conditions显示文摘 | Higa O Z Rogero S O Machado L D B | 1999 | Radiation Physics and Chemistry1999，55，56： | 1 |
| 6 | Clinical failure of vancomycin treatment of Staphylococcus aureus infection in A Tertiary Care Hespital in-seuthem Brazil显示文摘 | Lutz L Machado A Kuplich N | 2003 | Braz J Infect Dis2003，7，： | 1 |
| 7 | Towards property oriented testing 显示文摘 | MACHADO P D L SILVA D A MOTA A C | 2007 | Electrical Notes in Theoretical Computer Science2007，184，： | 1 |
| 8 | Aquaporins in the central nervous system显示文摘 | Venero J L Vizuete M L Machado A | 2001 | Prog Neurobiol2001，63，3： | 1 |
| 9 | Floating photocatalysts based on TiO2 supported on high surface area exfoliated vermiculite for water decontamination显示文摘 | Machado L C R Torchia C B Lago R M | 2006 | Catal Com2006，7，： | 1 |
| 10 | Cost- effectiveness of linezolid versus vancomycin in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant Staphylococcus aureus显示文摘 | Machado A R L Arns C C Follador W | 2005 | Braz J lnfect Dis2005，9，3： | 1 |
| 11 | Catalytic growth of carbon nanofibers on Cr nanoparticles on a carbon substrate:adsorbents for organic dyes in water显示文摘 | de Oliveira L C da Silva A C Machado A R T | | 0，，05： | 1 |
| 12 | Effect of milk fatand total solids concentration on the kinetics of moistureuptake by ready-to-eat breakfast cereal 显示文摘 | Machado M F Oliveira FAR Cunha L M | 1999 | InternationalJournal of Food Science and Technology1999，34，1： | 1 |
| 13 | Extraction of lemongrass essential oil with dense carbon dioxide显示文摘 | Carlson L H C Machado R A F Spricigo C B | 2001 | J Supercrit Fluids2001，21，： | 1 |
| 14 | DNA converts cellular prion protein into the β-sheet conformation and inhibits prion peptide aggregation 显示文摘 | Cordeiro Y Machado F Juliano L | 2002 | Journal of Biological Chemistry2002，276，49： | 1 |
| 15 | Tactics for vascular protection after acute ischemic stroke 显示文摘 | Fagan S C Hess D C Machado L S | 2005 | Pharmacotherapy2005，25，3： | 1 |
| 16 | Evaluation of insecticidal activity of diterpenes and lignans from Aristolochia malmeana against Anticarsia gemmatalis 显示文摘 | Messiano G B Vieira L Machado M B | 2008 | JAgric Food Chem2008，56，： | 1 |
| 17 | Cardiovascular risk in peritoneal dialysis-a Portuguese multicenter study显示文摘 | Neves M Machado S Rodrigues L | 2014 | Nefrologia2014，34，2： | 1 |
| 18 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 19 | Below -ground resourceslimit seedling growth in forest understories but do not alterbiomass distribution显示文摘 | Machado J L Walters M B | 2003 | Annual Forestry Science2003，60，： | 1 |
| 20 | Mechanical characterization of reactively magnetron-sputtered TiN films 显示文摘 | Vaz F Machado P Rebouta L | 2003 | Surface and Coatings Technology2003，，： | 1 |
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