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| 1 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Absorption of a- mino acids from the intestine and their netflux across the mesenteric -and portal -drained viscera of lambs显示文摘 | MACRAE J C BRUCE L A BROWN D S | 1997 | J Anim Sci1997，75，12： | 1 |
| 3 | Significance of the Properties of Materi- als in the Packing of Real Spherical Particles显示文摘 | Macrae J C Gray W A | 1961 | British Jour- nal of Applied Physics1961，12，4： | 1 |
| 4 | Effect of intravenous amino acid infusion on leucine oxidation across the mammary gland of the lactating goat显示文摘 | Bequette B J Backwell F R MacRae J C | 1996 | Journal of Dairy Science1996，79，： | 1 |
| 5 | Displacement-based design of RC Bridge columns in seismic region显示文摘 | KOWALSKY M J PRIESTLEY M J N MACRAE G A | 1995 | Earthquake Engineering and Structural Dynamics1995，24，： | 1 |
| 6 | Control of Listeria monocytogenes in salmon;antimicrobial effect of salting,smoking and specific smoke compounds显示文摘 | NIEDZIELA J MACRAE M OGDEN I | 1998 | Lebensmittel Wissenschaft Technologie1998，31，2： | 1 |
| 7 | Lysine metabolism by the mammary gland of lactating goats at two stages of lactation显示文摘 | Mabjeesh S J Kyle C E Macrae J C | 2000 | Journal of Dairy Science2000，83，： | 1 |
| 8 | Agrobacterium rhizogenes-mediated transforma- tion to improve rooting ability of eucalypts显示文摘 | Macrae S Staden J V | 1993 | Tree Physiology1993，12，4： | 1 |
| 9 | Influence of ischemia and reperfusion on the course of brain tissue swelling and blood-brain barrier permeability in a rodent model of transient focal cerebral ischemia显示文摘 | Gartshore G Patterson J Macrae IM | 1997 | Exp Neurol1997，147，： | 1 |
| 10 | Residual displacement response spectrum 显示文摘 | Kawashima K Macrae G A Hoshikuma J | 1998 | Journal of Structural Engineering (ASCE)1998，124，5： | 1 |
| 11 | DNA detection using en zymatic signal production and SERS显示文摘 | Dougan J A Macrae D Graham D | 2011 | Chem Commun2011，47，16： | 1 |
| 12 | Uptake of mercury by thiol-grafted chitosan gel beads 显示文摘 | Merrifield J Davids W MacRae J | 2004 | Water Research2004，38，13： | 1 |
| 13 | Structural Basis for Double-Stranded RNA Processing by Dicer显示文摘 | ZHOU KH LI F | 2006 | Science2006，311，5758： | 1 |
| 14 | Structural determinants of RNA recognition and cleavage by Dicer显示文摘 | MacRae I J Zhou K Doudna J A | 2007 | Nature Structural & Molecular Biology2007，14，10： | 1 |
| 15 | Thinking About Actions: The Neural Substrates of Person Knowledge 显示文摘 | Mason M F Banfield J F Macrae C N | 2004 | Cerebral Cortex2004，14，2： | 1 |
| 16 | Malignant mesothelioma:Canadian perspective and research directions显示文摘 | Lee CW Martin J MacRae R | 2008 | Curr Oncol2008，15，2： | 1 |
| 17 | Structural Basis for Double- Stranded RNA Processing by Dicer显示文摘 | MacRae I J Zbou K Li F | 2006 | Science2006，311，5758： | 1 |
| 18 | The effects of two stretching procedures on hip range of motion and gait econ- omy显示文摘 | CdODGES J J MACRAE H LONGDON C | 1989 | J Orthop Sport Phys1989，10，9： | 1 |
| 19 | Vascular sources of amino acids for milk protein synthesis in goats at two stages of lactation显示文摘 | Mabjeesh S J Kyle C E MacRae J C | 2002 | J Dairy Sci2002，85，4： | 1 |
| 20 | Conditioning 'Kensington' mango with hot air alleviates hot water disinfestation injuries 显示文摘 | Jacobi K Giles J MacRae E | 1995 | HortScience1995，30，： | 1 |
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