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| 1 | Exogenous cytokinin application to Actinidia chinensis var. deliciosa ‘Hayward’ fruit promotes fruit expansion through water uptake显示文摘Exogenous application of a cytokinin-like compound forchlorfenuron(CPPU)can promote fruit growth,although often at the expense of dry matter(DM),an important indicator of fruit quality.Actinidia chinensis var.deliciosa‘Hayward’fruit are very responsive to CPPU treatments,but the mechanism underlying the significant fruit weight increase and associated decrease in DM is unclear.In this study,we hypothesised that CPPU-enhanced growth increases fruit carbohydrate demand,but limited carbohydrate supply resulted in decreased fruit DM.During fruit development,CPPU effects on physical parameters,metabolites,osmotic pressure and transcriptional changes were assessed under conditions of both standard and a high carbohydrate supply.We showed that CPPU increased fruit fresh weight but the dramatic DM decrease was not carbohydrate limited.Enhanced glucose and fructose concentrations contributed to an increase in soluble carbohydrate osmotic pressure,which was correlated with increased water accumulation in CPPU-treated fruit and up-regulation of water channel aquaporin gene PIP2.4 at 49 days after anthesis.Transcipt analysis suggested that the molecular mechanism contributing to increased glucose and fructose concentrations was altered by carbohydrate supply.At standard carbohydrate supply,the early glucose increase in CPPU fruit was associated with reduced starch synthesis and increased starch degradation.When carbohydrate supply was high,the early glucose increase in CPPU fruit was associated with a general decrease in starch synthesis but up-regulation of vacuolar invertase and fructokinase genes.We conclude that CPPU affected fruit expansion by increasing the osmotically-driven water uptake and its effect was not carbohydrate supply-limited. | Simona Nardozza Helen L Boldingh Mark W Wohlers Andrew P Gleave Zhiwei Luo Guglielmo Costa Elspeth A MacRae Michael J Clearwater Annette C Richardson | 2017 | Horticulture Research2017，4，1： | 2 |
| 2 | Absorption of a- mino acids from the intestine and their netflux across the mesenteric -and portal -drained viscera of lambs显示文摘 | MACRAE J C BRUCE L A BROWN D S | 1997 | J Anim Sci1997，75，12： | 1 |
| 3 | Significance of the Properties of Materi- als in the Packing of Real Spherical Particles显示文摘 | Macrae J C Gray W A | 1961 | British Jour- nal of Applied Physics1961，12，4： | 1 |
| 4 | Effect of intravenous amino acid infusion on leucine oxidation across the mammary gland of the lactating goat显示文摘 | Bequette B J Backwell F R MacRae J C | 1996 | Journal of Dairy Science1996，79，： | 1 |
| 5 | Missense mutations in the rod domain of the lamin A/C gene as causes of dilated cardiomyopathy and conduction-system disease显示文摘 | Fatkin D MacRae C Sasaki T | 1999 | N Engl J Med1999，341，23： | 1 |
| 6 | Carbohydrate metabolism during postharvest ripening in kiwifruit显示文摘 | MACRAE E QUICK W P BENKER C | 1992 | Planta1992，188，： | 1 |
| 7 | Dynamic Response and Fatigue of Steel Tied-arch Brldge显示文摘 | Roeder C MacRae G Crocker P et a l | 2000 | Journal of Bridge Engi neering2000，5，1： | 1 |
| 8 | Lysine metabolism by the mammary gland of lactating goats at two stages of lactation显示文摘 | Mabjeesh S J Kyle C E Macrae J C | 2000 | Journal of Dairy Science2000，83，： | 1 |
| 9 | Hypertrophic cardionyopathy:A discussion of nomenclature显示文摘 | Maron BJ Epstein SE MacRae C | 1979 | Am J Cardiol1979，43，6： | 1 |
| 10 | α-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: A disease of the sarcomere 显示文摘 | Thierfelder L Watkins H MacRae C | 1994 | Cell1994，77，： | 1 |
| 11 | Mitochondrial substrate availability and its role in lipid-induced insulin resistance and proinflammatory signaling in skeletal muscle显示文摘 | Lipina C Macrae K Suhm T | 2013 | Diabetes2013，62，10： | 1 |
| 12 | Thinking About Actions: The Neural Substrates of Person Knowledge 显示文摘 | Mason M F Banfield J F Macrae C N | 2004 | Cerebral Cortex2004，14，2： | 1 |
| 13 | Biodegradation of feather waste by extracellular kera-tinases and gelatinases from Bacillus spp显示文摘 | MAZOTTO A M MELO A C N D MACRAE A | 2011 | WorldJournal of Microbiology and Biotechnology2011，27，6： | 1 |
| 14 | The effects of two stretching procedures on hip range of motion and gait econ- omy显示文摘 | CdODGES J J MACRAE H LONGDON C | 1989 | J Orthop Sport Phys1989，10，9： | 1 |
| 15 | Vascular sources of amino acids for milk protein synthesis in goats at two stages of lactation显示文摘 | Mabjeesh S J Kyle C E MacRae J C | 2002 | J Dairy Sci2002，85，4： | 1 |
| 16 | Near-fault ground motion effects on simple structures显示文摘 | Macrae G A Morrow D V and Roeder C W | 2001 | Structure Engineering2001，127，9： | 1 |
| 17 | Effect of column stiffness on braced frame seismic behavior 显示文摘 | MacRae G A Kimura Y Roeder C W | 2004 | Journal of Strucral Engineering2004，130，3： | 1 |
| 18 | Why self- control seems (but may not be) limited显示文摘 | INZLICHT M SCHMEICHEL B J MACRAE C N | 2014 | Trends Cogn Sci2014，18，3： | 1 |
| 19 | Lysine metabolism by the mammary gland of lactating goats at two stages of lactation显示文摘 | Mabjeesh S J Kyle C E Macrae J C Bequette B J | 2000 | Journal of Dairy Science2000，83，： | 1 |
| 20 | Separate effects of the microkeratome incision and laser ablation on the eye's wave aberration显示文摘 | Porter J MacRae S Yoon G Roberts C Cox IG Williams DR | 2003 | Am J Ophthalmol2003，136，2： | 1 |
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