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| 1 | Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons显示文摘AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ileum.METHODS:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal plexus.The experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and calretinin.RESULTS:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR groups.Double labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 receptor.In the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 receptor.In the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D group.The spatial density of calbindin-IR neurons,however,did not differ among the three groups.The submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR neurons.The average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR neurons.CONCLUSION:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrinsic primary afferent,cholinergic secretomotor and vasomotor neurons.Undernutrition affected P2X2 receptor expression in the submucosal plexus,and neuronal and size.These changes were rescued in the re-fed rats. | Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno Edson Aparecido Liberti John Barton Furness Patricia Castelucci | 2010 | World Journal of Gastroenterology2010，16，29： | 2 |
| 2 | Antitumor activity of arginine deiminase from mycoplasma arginini and its growth-inhibitory mechanism显示文摘 | TAKAKU H MATSUMOTO M MISAWA S | 1995 | Japanese Journal of Cancer Research1995，86，9： | 1 |
| 3 | Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus显示文摘 | Nishibuchi M Taniguchi T Misawa T | 1989 | Infect Immun1989，57，： | 1 |
| 4 | Aloe vera phytosterols act as ligands for PPAR and improve the ex- pression levels of PPAR target genes in the livers of mice with diet-induced obesity 显示文摘 | Nomaguchi K Tanaka M Misawa E | 2011 | Obesity Research & Clini- cal Practice2011，5，3： | 1 |
| 5 | Role of P-glycoprotein in human natural killer-like cell line- mediated cytotoxicity显示文摘 | Takahashi M Misawa Y Watanabe N | 1999 | Exp Cell Res1999，253，2： | 1 |
| 6 | Development and industrialization of new intermediate 3-chloromethyl-Δ3-cephems for cephaiosporin antibiotic synthesis显示文摘 | TANIGUCHI M MISAWA T SHIROI T | 1995 | Nippon Kagaku Kaishi1995，，8： | 1 |
| 7 | Structure of Protec- tive Rust Layers Formed on Weathering Steels by Long-Tema Exposure in the Industrial Atmospheres of Japan and North America显示文摘 | Yamashita M Nagano H Misawa T | 1998 | ISIJ International1998，38，3： | 1 |
| 8 | Potential role of Regucalcin as a specific biochemical marker of chronic liver injury with carbon tetrachloride administration in rats显示文摘 | Tsurusaki Y Misawa H | 2002 | Mol Cell Biochem2002，241，12： | 1 |
| 9 | Elucidation of the Erwinia uredovora carotenoid biosynthesis pathway by functional analysis of gene products expressed in Escherichia coli显示文摘 | MISAWA N NAKAGAWA M KOBAYASHI K | 1990 | J Bacteriol1990，172，12： | 1 |
| 10 | Simultaneous determination of twelve benzodiazepines in human serum using a new reversed-phase chromatographic column on a 2-μm porous microspherical silica gel显示文摘 | Tanaka E Terada M Misawa S | 1996 | J Chromatogr1996，682，： | 1 |
| 11 | Metabolic engineering of ketocarotenoid formation in higher plants 显示文摘 | Ralley L Enfissi E M A Misawa N | 2004 | Plant J2004，39，： | 1 |
| 12 | The clinical mani- festations of lumbar disease are correlated with self-rating depression scale scores 显示文摘 | Tetsunaga T Misawa H Tanaka M | 2013 | J Orthop Sci2013，27，： | 1 |
| 13 | Vascular pattern classiflica- tion of colorectal lesions with narrow band imaging magnifying endoscopy显示文摘 | Wada Y Kudo SE Misawa M | 2011 | Dig Endosc2011，23，1： | 1 |
| 14 | Analytical and experimental investigations for satellite antenna deployment mechanisms显示文摘 | MISAWA M YASAKA T MIYAKE S | 1989 | Journal of Spacecraft and Rockets1989，26，3： | 1 |
| 15 | Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli显示文摘 | Misawa N Nakagawa M Kobayashi K | | 0，，12： | 1 |
| 16 | Involvement of L-type Ca(2 + )channels in the induction of long-term potentiation in the basolateral amygdalar dentate gyrus pathway of anesthetized rats显示文摘 | Niikura Y Abe K Misawa M | 2004 | Brain Res2004，1017，12： | 1 |
| 17 | MicroRNAs and their therapeutic potential forhuman diseases: MiR-133a andbronchial smooth muscle hyperre-sponsiveness in asthma 显示文摘 | Chiba Y Misawa M | 2010 | J Pharmacol Sci2010，114，3： | 1 |
| 18 | MicroRNAs and their therapeutic potential for human diseases: MiR-133a and bronchial smooth muscle hy- perresponsiveness in asthma 显示文摘 | Chiba Y Misawa M | 2010 | J Pharmacol Sci2010，114，3： | 1 |
| 19 | Negative Thermal Ex-pansion in Ge-Free Antiperovskite Manganese Nitrides : Tin-DopingEffect 显示文摘 | Takenaka K Asano K Misawa M | 2008 | Applied Physics Letters2008，011，927： | 1 |
| 20 | Effects of chemical and physical on growth of Camptotheca acuminata cell culture显示文摘 | Saka T K Misawa M | 1974 | Agr Bio Chem1974，38，3： | 1 |
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