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| 1 | Microbiota modification by probiotic supplementation reduces colitis associated colon cancer in mice显示文摘AIM To investigate the effect of probiotic supplementation during the development of an experimental model of colitis associated colon cancer(CAC). METHODS C57 BL/6 mice received an intraperitoneal injection of azoxymethane(10 mg/kg), followed by three cycles of sodium dextran sulphate diluted in water(5% w/v). Probiotic group received daily a mixture of Lactobacillus acidophilus, Lactobacil us rhamnosus and Bifidobacterium bifidum. Microbiota composition was assessed by 16 Sr RNA Illumina Hi Seq sequencing. Colon samples were collected for histological analysis. Tumor cytokines was assessed by Real Time-PCR(Polymerase Chain Reaction); and serum cytokines by Multiplex assay. All tests were two-sided. The level of significance was set at P < 0.05. Graphs were generated and statistical analysis performed using the software Graph Pad Prism 5.0. The project was approved by the institutional review board committee. RESULTS At day 60 after azoxymethane injection, the mean number of tumours in the probiotic group was 40% lower than that in the control group, and the probiotic group exhibited tumours of smaller size(< 2 mm)(P < 0.05). There was no difference in richness and diversity between groups. However, there was a significant difference in beta diversity in the multidimensional scaling analysis. The abundance of the genera Lactobacillus, Bifidobacterium, Allobaculum, Clostridium XI and Clostridium XVⅢ increased in the probiotic group(P < 0.05). The microbial change was accompanied by reduced colitis, demonstrated by a 46% reduction in the colon inflammatory index; reduced expression of the serum chemokines RANTES and Eotaxin; decreased p-IKK and TNF-α and increased IL-10 expression in the colon. CONCLUSION Our results suggest a potential chemopreventive effect of probiotic on CAC. Probiotic supplementation changes microbiota structure and regulates the inflammatory response, reducing colitis and preventing CAC. | Maria Carolina S Mendes Daiane SM Paulino Sandra R Brambilla Juliana A Camargo Gabriela F Persinoti José Barreto C Carvalheira | 2018 | World Journal of Gastroenterology2018，24，18： | 17 |
| 2 | Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells显示文摘AIM:To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells. METHODS: Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. RESULTS: Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P < 0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P < 0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P < 0.05) adhesion to GP202 clones with larger MUC1 VNTR domains. CONCLUSION: This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of theMUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa. | Natália R Costa Nuno Mendes Nuno T Marcos Celso A Reis Thomas Caffrey Michael A Hollingsworth Filipe Santos-Silva | 2008 | World Journal of Gastroenterology2008，14，9： | 4 |
| 3 | An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antibodies 显示文摘 | Allende R Sepulveda L Mendes da Silva A | 1992 | Prev Vet Med1992，14，： | 1 |
| 4 | Effect of frozen storage on thechemical and physical properties of black and silver scabbard fish显示文摘 | DAIAS J NUNES M L MENDES R | 1994 | Journal of the Science of Food and Agriculture1994，66，3： | 1 |
| 5 | The fully informed particle swarm: Simpler, maybe better显示文摘 | Mendes R Kennedy J Neves J | 2004 | IEEE Trans on Evolutionary Computation2004，8，3： | 1 |
| 6 | The role of constraints within generalized nonextensive statistics显示文摘 | Tsallis C Mendes R S Plastino A R | 1998 | Physica A:Statistical Mechanics and its Applications1998，261，3： | 1 |
| 7 | Coverage of the left subclavian artery during thoracic endovascular aortic repair 显示文摘 | Riesenman P J Farber M A Mendes R R | 2007 | J Vasc Surg2007，45，1： | 1 |
| 8 | Ectopic preg- nancies in a caesarean scar: review of the medical approach to an iatrogenic complication显示文摘 | Mayrnon R Halperin R Mend|ovic S eta| | 2004 | Hum Reprod Update2004，10，： | 1 |
| 9 | Crypthecodinium cohnii with emphasis on DHA production:a review显示文摘 | Mendes A Reis A Vasconcelos R | 2009 | J Appl Phycol2009，21，2： | 1 |
| 10 | Effect of transglutaminaseand carrageenan on restructured fish products containing dietaryfibres 显示文摘 | Cardoso C Mendes R Nunes M L | 2007 | International Journal of Food Science and Technology2007，42，： | 1 |
| 11 | The effect of supplementation with expanded sunflower seed on carcass and meat quality of lambs raised on pasture 显示文摘 | Santos Silva J Bessa R J B Mendes I A | 2003 | Meat Science2003，65，： | 1 |
| 12 | Parameter estimation in biochemical pathway: a comparison of global optimization methods显示文摘 | Moles C G Mendes P Banga J R | 2003 | Genome Research2003，13，： | 1 |
| 13 | Pharmacodynamic comparisons of antimicrobials against nosocomial isolates of escherichia coli,klebsiella pneumoniae,acinetobacter baumannii and pseudomonas aeruginosa from the MYSTIC surveillance program:the OPTAMA Program,South America 2002显示文摘 | KIFFER C R MENDES C KUTI J L | 2004 | Diagn Microbiol Infect Dis2004，49，2： | 1 |
| 14 | Neighborhood topologies in fully informed and best-of-neighborhood particle swarm 显示文摘 | KENNEDY J MENDES R | 2006 | Systems Man and Cybernetics Part C:Applications and Reviews IEEE Transactions:20062006，36，4： | 1 |
| 15 | Development of a healthylow-fat fishsausage containing dietary fibre 显示文摘 | Cardoso C Mendes R Nunes M L | 2008 | InternationalJournal of Food Science andTechnology2008，43，： | 1 |
| 16 | Load-independent parameters of diastolic and systolic function by speckle tracking and tissue doppler in hemodialysis patients 显示文摘 | Mendes L Ribeiras R Adragao T | 2008 | Rev Port Cardiol2008，27，9： | 1 |
| 17 | Obstruction of pipelines due to paraffin deposition during the flow of crude oils显示文摘 | Ribeiro F S Mendes P R S Braga S L | 1997 | International Journal of Heat Mass Transfer1997，18，1： | 1 |
| 18 | Implications of the use of sorghum in broiler production显示文摘 | GARCIA R G MENDES A A ALMEIDA P | 2013 | Revista Brosileira De Ciencia Avicola2013，15，3： | 1 |
| 19 | Jelleines: a family of antimicrobial peptldes from the royal jelly of honeybees (Ap/s ellifera)显示文摘 | FONTANA R MENDES M A SOUZA B M | 2004 | Veptides2004，25，6： | 1 |
| 20 | Optimal scheduling of spinning reserve 显示文摘 | Gooi H B Mendes D P Be11 K R W | 1999 | IEEE Transactions on Power Systems1999，14，4： | 1 |
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