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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Pancreatic ductal system obstruction and acute recurrent pancreatitis显示文摘Acute recurrent pancreatitis is a clinical entity largely associated with pancreatic ductal obstruction. This latter includes congenital variants, of which pancreas divisum is the most frequent but also controversial, chronic pancreatitis, tumors of the pancreaticobiliary junction and sphincter of Oddi dysfunction. This review summarizes current knowledge about diagnostic work-up and therapy of these conditions. | M Delhaye C Matos M Arvanitakis J Devière | 2008 | World Journal of Gastroenterology2008，14，7： | 12 |
| 3 | Parent-child interaction therapy for Puerto Rican preschool children with ADHD and behavior problems: a pilot efficacy study显示文摘 | Matos M Bauermeister J J Bernal G | 2009 | Faro Process2009，48，2： | 1 |
| 4 | Neuroendocrine gastric carcino- ma expressing somatostain: a highly malignant, rare tumor 显示文摘 | Waisberg J de Matos LL Pezzolo S | 2006 | World J Gastroentero12006，12，24： | 1 |
| 5 | Pancreatic acinar cell carcinoma: a multi-institutional study 显示文摘 | Matos J M Schmidt C M Turrini O | 2009 | J Gastrointest Surg2009，13，8： | 1 |
| 6 | Enthalpies of combustion, vapour pressures, and enthalpies of sublimation of 8- hydroxyquinoline, 5-nitro-8-hydroxyquinoline, and 2-methyl-8-hydroxyquinoline显示文摘 | Ribeiro Da Silva M A V Monte M J S Matos M A R | 1989 | Chem Thermodyn1989，，21： | 1 |
| 7 | Endoscopic management of chronic pancreatitis 显示文摘 | Delhaye M Matos C Deviere J | 2003 | Gastrointest Endose Clin N Am2003，13，4： | 1 |
| 8 | Dehydration studies of rare earth p-toluenesulfonate hydrates by TG/DTG and DSC 显示文摘 | Santos A V Matos J R | 2002 | Al- loyscomp2002，344，12： | 1 |
| 9 | Surface nano-aggregation and photocatalytic activity of Ti O2onH-type activated carbons显示文摘 | Cordero T Chovelon J M Duchamp C Ferronato C Matos J | 2007 | Applied Catalysis B:Environmental2007，73，22： | 1 |
| 10 | Altered glycogen metabolism in the submandibular and parotid salivary glands of rats with streptozotocin-induced diabetes显示文摘 | Nicolau J de Matos JA de Souza DN | | 0，，02： | 1 |
| 11 | S-adenosylmethionine in alcoholic liver cirrhosis: a randomized, placebo-controlled, double-blind, multicenter clinical trial显示文摘 | Mato JM Cámara J Fernández de Paz J | 1999 | J Hepatol1999，30，6： | 1 |
| 12 | Influence of limestone addition on the behavior of NO and N20 dur- ing fluidised bed coal combustion 显示文摘 | Tarelho L A C Matos M A A Pereira F J M A | 2006 | Fuel2006，85，7： | 1 |
| 13 | Ultrasound -assisted treatment of pahn oil samples for the determination of copper and lead by stripping chronopotenfiometry显示文摘 | Cypriano J C Matos M A C Mates R C | 2008 | Microchem J2008，90，： | 1 |
| 14 | Enzymatic hydrolysis of waste paper显示文摘 | OLTUS E MATO J BAUER S | 1987 | Cellulose Chem And Technol1987，21，： | 1 |
| 15 | Benefits of donor solvents as additive on ROMP of norbornene catalyzed by amine Ru complexes显示文摘 | Matos J M E Lima-Neto B S | 2005 | Molecular Catalysis A: Chemical2005，240，： | 1 |
| 16 | Activated carbon supported Ni-Ca:Influence of reaction parameters on activity and stability of catalyst on methane reformation显示文摘 | D?az K Garc?a V Matos J | 2007 | Fuel2007，86，9： | 1 |
| 17 | Thermal characterization of mica-epoxy composite used as insulation material for high voltage machines显示文摘 | Koreeda T Matos J | 2011 | Journal of Thermal Analysis and Calorimetry2011，106，2： | 1 |
| 18 | The fibroblast growth factor family:involvement in the regulation of folliculogenesis显示文摘 | Chaves RN de Matos MH Buratini J Jr | 2012 | Reprod Fertil Dev2012，24，7： | 1 |
| 19 | Copropagating and counterpropagating pumps in second-order-pumped discrete fiber Raman amplifiers显示文摘 | Chestnut D A de Matos C J S Reeves-Hall P C | 2002 | Optics Letters2002，27，19： | 1 |
| 20 | Choledochal cysts:comparison of findings at MR cholangiopancreatography and endo,opic retrograde cholangiopancreatography in eight patients显示文摘 | Matos C Nicaise N Deviere J | 1998 | Radiology1998，209，2： | 1 |
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