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| 1 | An Overview of Autism Spectrum Disorder, Heterogeneity and Treatment Options显示文摘Since the documented observations of Kanner in1943, there has been great debate about the diagnoses, the sub-types, and the diagnostic threshold that relates to what is now known as autism spectrum disorder(ASD).Re?ecting this complicated history, there has been continual re?nement from DSM-III with ‘Infantile Autism' to the current DSM-V diagnosis. The disorder is now widely accepted as a complex, pervasive, heterogeneous condition with multiple etiologies, sub-types, and developmental trajectories. Diagnosis remains based on observation of atypical behaviors, with criteria of persistent de?cits in social communication and restricted and repetitive patterns of behavior. This review provides a broad overview of the history, prevalence, etiology, clinical presentation, and heterogeneity of ASD. Factors contributing to heterogeneity, including genetic variability, comorbidity, and gender are reviewed. We then explore current evidencebased pharmacological and behavioral treatments for ASD and highlight the complexities of conducting clinical trials that evaluate therapeutic ef?cacy in ASD populations.Finally, we discuss the potential of a new wave of research examining objective biomarkers to facilitate the evaluation of sub-typing, diagnosis, and treatment response in ASD. | Anne Masi Marilena M.De Mayo Nicholas Glozier Adam J.Guastella | 2017 | Neuroscience Bulletin2017，33，2： | 33 |
| 2 | 极具工程化潜力的好氧颗粒污泥技术显示文摘介绍了形成好氧颗粒污泥的工艺条件及其在去除有机物、脱氮、除磷方面的最新研究进展,并总结了好氧颗粒污泥的代谢机理。与传统的活性污泥法相比,好氧颗粒污泥反应器具有微生物浓度高、沉淀速度快、占地面积小等独特优点,在未来几年内该技术将得到实际应用。 | 郝晓地 陈新华 戴吉 M.de Kreuk M.C.M.van Loosdrecht | 2006 | 中国给水排水2006，22，8： | 22 |
| 3 | NETosis,complement,and coagulation:a triangular relationship显示文摘NETosis is a regulated form of neutrophil cell death that contributes to the host defense against pathogens and was linked to various diseases soon after its first description in 2004.During NETosis,neutrophils release neutrophil extracellular traps(NETs),which can capture and kill bacteria and other pathogens to prevent them from spreading.Although substantial progress has been made in our understanding of NETosis,the precise mechanism underlying NETosis is still a matter of debate.Research continues to elucidate the molecular pathways involved in NETosis.In recent years,interactions with the complement and coagulation systems have become increasingly apparent.Activated complement proteins can stimulate NET formation,and NETs,in turn,can serve as a platform for complement activation.In addition,NETs can act as a scaffold for thrombus formation during coagulation.While crosstalk between the coagulation and complement systems has been previously described,NETosis appears to be a third important player in this consortium to protect the host against pathogens.This review summarizes our current knowledge on the mutual interactions between NETosis,the complement system and the coagulation system,with an emerging description of their complex triangular relationship. | Cynthia M.de Bont Wilbert C.Boelens Ger J.M.Pruijn | 2019 | Cellular & Molecular Immunology2019，16，1： | 22 |
| 4 | 冠状动脉内影像学临床应用专家共识(第一部分):对冠状动脉介入治疗的指导与优化显示文摘欧洲心血管介入协会(EAPCI)专家组系统总结了血管内超声(IVUS)和光学相干断层成像(OCT)这两种血管内影像学检查临床应用指征的现有证据,提供了关于IVUS和OCT指导经皮冠状动脉介入治疗(PCI)的应用价值,并明确了最可能从腔内影像学指导的介入治疗中获得临床收益的患者或病变类型,同时详细论述了PCI前如何使用IVUS或OCT优化支架尺寸(支架长度和直径)和手术策略的选择。此外,专家推荐对支架失败(支架内再狭窄或支架内血栓形成)的患者应常规进行冠状动脉内影像学检查,并首选OCT。最后,重点论述了IVUS和OCT在指导PCI和评估支架失败两个方面的优势和局限性,并对未来需要深入研究的领域进行了展望。 | Lorenz Raber Gary S Mintz Konstantinos C Koskinas Thomas W Johnson Niels R Holm Yoshinubo Onuma Maria D Radu Michael Joner Bo Yu Haibo Jia Nicolas Meneveau Jose M.de la Torre Hemandez Javier Escaned Jonathan Hill Francesco Prati Antonio Colombo Carlo di Mario Evelyn Regar Davide Capodanno William Wijns Robert A Byme Giulio Guagliumi | 2019 | 中华心血管病杂志2019，47，1： | 17 |
| 5 | Contemporary diagnostics and treatment options for female stress urinary incontinence显示文摘Stress urinary incontinence is not a deadly disease,but for the large population of women suffering from it,it is a very important issue.Especially in the continuously aging population all over the world,there is more and more need for treatment of this serious medical condition.Treatment of female stress urinary incontinence exists already for ages.In the 20th century invasive treatments like Burch colposuspension and pubovaginal slings were the mainstay of surgical treatments.The introduction of the midurethral sling made the procedure less invasive and accessible for more caregivers.Luckily there are many options available and the field is developing quickly.In recent years many new medical devices have been developed,that increase the number of treatment options available and make it possible to find a suitable solution for the individual patient based on subjective and objective results and the chances of complications.This manuscript provides an introduction to the therapeutical options that are available nowadays for female stress urinary incontinence. | Allert M.de Vries John P.F.A.Heesakkers | 2018 | Asian Journal of Urology2018，5，3： | 12 |
| 6 | 绿肥分解产物中水溶性有机物质的伏安行为及其影响因素显示文摘绿肥分解产物中的水溶性有机物质的峰数和电流值随绿肥培养时间和种类而异。分子量为120—130和140—160的水溶性有机物质具有明显的伏安行为,大于160者则贡献较小或无贡献。pH、亚铁和土壤类型对水溶性有机物质的伏安行为有显著影响。 | 丁昌璞 M.De Nobili B.Ceccanti | 1989 | 土壤学报1989，26，4： | 11 |
| 7 | Metastatic prostate cancer remains incurable, why?显示文摘Metastatic prostate cancer patients present in two waysdwith already disseminated disease at the time of presentation or with disease recurrence after definitive local therapy.Androgen deprivation therapy is given as the most effective initial treatment to patients.However,after the initial response,almost all patients will eventually progress despite the low levels of testosterone.Disease at this stage is termed castration resistant prostate cancer(CRPC).Before 2010,the taxane docetaxel was the first and only life prolonging agent for metastatic CRPC(mCRPC).The last decade has witnessed robust progress in CRPC therapeutics development.Abiraterone,enzalutamide,apalutamide and sipuleucel-T have been evaluated as first-and second-line agents in mCRPC patients,while cabazitaxel was approved as a second-line treatment.Radium-223 dichloride was approved in symptomatic patients with bone metastases and no known visceral metastases pre-and post-docetaxel.However,despite significant advances,mCRPC remains a lethal disease.Both primary and acquired resistance have been observed in CRPC patients treated by these new agents.It could be solely cell intrinsic or it is possible that the clonal heterogeneity in treated tumors may result from the adaptive responses to the selective pressures within the tumor microenvironment.The aim of this review is to list current treatment agents of CRPC and summarize recent findings in therapeutic resistance mechanisms. | Liang Dong Richard C.Zieren Wei Xue Theo M.de Reijke Kenneth J.Pienta | 2019 | Asian Journal of Urology2019，6，1： | 9 |
| 8 | 热装热送计划问题的解决方案显示文摘热装热送要求连铸机和轧机的生产计划相匹配。介绍一种采用先进算法自动生成热装计划的新系统,根据产品大纲和市场条件,热装比可达85%。目前该系统正在中国一家钢铁企业进行测试,目标是达到80%的热装比。提高热装比需要炼钢和轧钢工序的密切协调,需要操作、维修和计划各个部门的全面配合。 | P.Knoop M.De Feyter L.V.Nerom | 2006 | 钢铁2006，41，1： | 8 |
| 9 | 有穷固定模的确定与消除显示文摘本文研究带前馈广义分散控制大系统有穷固定模的确定与消除问题.给出一种不依赖于系统特征值确定与消除有穷固定模的方法. | 张庆灵 戴冠中 M.de la San | 1997 | 控制理论与应用1997，14，3： | 8 |
| 10 | Multiple responses optimization in the development of a headspace gas chromatography method for the determination of residual solvents in pharmaceuticals显示文摘An efficient generic static headspace gas chromatography(HSGC) method was developed,optimized and validated for the routine determination of several residual solvents(RS) in drug substance,using a strategy with two sets of calibration.Dimethylsulfoxide(DMSO) was selected as the sample diluent and internal standards were used to minimize signal variations due to the preparative step.A gas chromatograph from Agilent Model 6890 equipped with flame ionization detector(FID) and a DB-624(30 m × 0.53 mm i.d.,3.00 μm film thickness) column was used.The inlet split ratio was 5:1.The influencing factors in the chromatographic separation of the analytes were determined through a fractional factorial experimental design.Significant variables:the initial temperature(IT),the final temperature(FT) of the oven and the carrier gas flow rate(F) were optimized using a central composite design.Response transformation and desirability function were applied to find out the optimal combination of the chromatographic variables to achieve an adequate resolution of the analytes and short analysis time.These conditions were 30 ℃ for IT,158 ℃ for FT and 1.90 mL/min for F.The method was proven to be accurate,linear in a wide range and very sensitive for the analyzed solvents through a comprehensive validation according to the ICH guidelines. | Carla M.Teglia Milagros Montemurro María M.De Zan María S.Cmara | 2015 | Journal of Pharmaceutical Analysis2015，5，5： | 8 |
| 11 | 重症肌无力单链抗体基因的制备显示文摘[目的 ]构建抗人乙酰胆碱受体主要免疫原区单链抗体 .[方法 ]应用多聚酶链反应从抗人乙酰胆碱受体主要免疫原区抗原结合片段扩增重链和轻链可变区基因 ,并克隆到载体噬粒 pHEN2 .将含有单链抗体基因的重组噬粒转化大肠杆菌DH5α ,分离提纯重组噬粒后再经酶切、琼脂糖凝胶电泳检查以确认单链抗体基因的正确性 .[结果 ]电泳检查发现了大小分别为 3 78bp ,3 3 9bp和 762bp的重链可变区、轻链可变区和单链抗体基因 ,且位置正确 .[结论 | 杨康鹃 孟繁平 M.Stassen M.de Baets | 2002 | 延边大学医学学报2002，25，1： | 6 |
| 12 | High-speed water impacts of flat plates in different ditching configuration through a Riemann--ALE SPH model显示文摘The violent water entry of flat plates is investigated using a Riemann-arbitrary Eulerian-Lagrangian(ALE) smoothed particle hydrodynamics(SPH) model. The test conditions are of interest for problems related to aircraft and helicopter emergency landing in water. Three main parameters are considered: the horizontal velocity, the approach angle(i.e., vertical to horizontal velocity ratio) and the pitch angle, a. Regarding the latter, small angles are considered in this study. As described in the theoretical work by Zhao and Faltinsen(1993), for small a a very thin, high-speed jet of water is formed, and the time-spatial gradients of the pressure field are extremely high. These test conditions are very challenging for numerical solvers. In the present study an enhanced SPH model is firstly tested on a purely vertical impact with deadrise angle α=4°. An in-depth validation against analytical solutions and experimental results is carried out, highlighting the several critical aspects of the numerical modelling of this kind of flow, especially when pressure peaks are to be captured. A discussion on the main difficulties when comparing to model scale experiments is also provided. Then, the more realistic case of a plate with both horizontal and vertical velocity components is discussed and compared to ditching experiments recently carried out at CNR-INSEAN. In the latter case both 2-D and 3-D simulations are considered and the importance of 3-D effects on the pressure peak is discussed for α=4° and α=10°. | S.Marrone A.Colagrossi L.Chiron M.De Leffe D.Le Touzé | 2018 | Journal of Hydrodynamics2018，30，1： | 5 |
| 13 | 益生元和益生菌显示文摘益生元是一种不易被消化的可食用物质,通过选择性地刺激肠道中有益细菌的生长,从而对宿主产生有益的影响。益生菌是有益微生物的活菌制剂。有关益生元和益生菌的生长性能的试验结果并不一致。需要更多的研究来证明益生元和益生素在猪日粮中使用的合理性。 | 张婵娟 Jay Y.Jacela Joel M.De Rouchey Mike D.Tokach | 2011 | 国外畜牧学(猪与禽)2011，，6： | 4 |
| 14 | 电鳐乙酰胆碱受体的分离和纯化显示文摘[目的 ]分离和纯化具有活性的电鳐乙酰胆碱受体 .[方法 ]将电鳐电器官肌肉制成匀浆 ,细胞经裂解制备电鳐乙酰胆碱受体粗提液 .经眼镜蛇毒素偶联的Sepharose 4B层析柱亲和吸附 ,得到纯化的电鳐乙酰胆碱受体 .应用酶联免疫吸附试验检查纯化的电鳐乙酰胆碱受体与抗乙酰胆碱受体抗体的结合活性 .[结果 ]分离和纯化的电鳐乙酰胆碱受体能与抗乙酰胆碱受体抗体结合 ,且结合活性高 .[结论 ]从电鳐电器官中分离和纯化的乙酰胆碱受体活性高、特异性强 ,可作为抗原在酶联免疫吸附试验中用于检查抗乙酰胆碱受体抗体 . | 杨康鹃 孟繁平 M.de Baets | 2004 | 延边大学医学学报2004，27，1： | 3 |
| 15 | Graphene-based Q-switched pulsed fiber laser in a linear configuration显示文摘A pulsed laser system is realized with graphene employed as a Q-switch.The graphene is exfoliated from its solution using an optical deposition and the optical tweezer effect.A fiber ferrule that already has the graphene deposited on it is inserted into an erbium-ytterbium laser(EYL)system with linear cavity configuration.We successfully demonstrate a pulsed EYL with a pulse duration of approximately 5.9μs and a repetition rate of 20.0 kHz. | Y.K.Yap Richard M.De La Rue C.H.Pua S.W.Harun H.Ahmad | 2012 | Chinese Optics Letters2012，10，4： | 3 |
| 16 | Fungal diversity notes 111-252-taxonomic and phylogenetic contributions to fungal taxa显示文摘This paper is a compilation of notes on 142 fungal taxa,including five new families,20 new genera,and 100 new species,representing a wide taxonomic and geographic range.The new families,Ascocylindricaceae,Caryosporaceae and Wicklowiaceae(Ascomycota)are introduced based on their distinct lineages and unique morphology.The new Dothideomycete genera Pseudomassariosphaeria(Amniculicolaceae),Heracleicola,Neodidymella and Pseudomicrosphaeriopsis(Didymellaceae),Pseudopithomyces(Didymosphaeriaceae),Brunneoclavispora,Neolophiostoma and Sulcosporium(Halotthiaceae),Lophiohelichrysum(Lophiostomataceae),Galliicola,Populocrescentia and Vagicola(Phaeosphaeriaceae),Ascocylindrica(Ascocylindricaceae),Elongatopedicellata(Roussoellaceae),Pseudoasteromassaria(Latoruaceae)and Pseudomonodictys(Macrodiplodiopsidaceae)are introduced.The newly described species of Dothideomycetes(Ascomycota)are Pseudomassariosphaeria bromicola(Amniculicolaceae),Flammeascoma lignicola(Anteagloniaceae),Ascocylindrica marina(Ascocylindricaceae),Lembosia xyliae(Asterinaceae),Diplodia crataegicola and Diplodia galiicola(Botryosphaeriaceae),Caryospora aquatica(Caryosporaceae),Heracleicola premilcurensis and Neodidymella thailandicum(Didymellaceae),Pseudopithomyces palmicola(Didymosphaeriaceae),Floricola viticola(Floricolaceae),Brunneoclavispora bambusae,Neolophiostoma pigmentatum and Sulcosporium thailandica(Halotthiaceae),Pseudoasteromassaria fagi(Latoruaceae),Keissleriella dactylidicola(Lentitheciaceae),Lophiohelichrysum helichrysi(Lophiostomataceae),Aquasubmersa japonica(Lophiotremataceae),Pseudomonodictys tectonae(Macrodiplodiopsidaceae),Microthyrium buxicola and Tumidispora shoreae(Microthyriaceae),Alloleptosphaeria clematidis,Allophaeosphaeria cytisi,Allophaeosphaeria subcylindrospora,Dematiopleospora luzulae,Entodesmium artemisiae,Galiicola pseudophaeosphaeria,Loratospora luzulae,Nodulosphaeria senecionis,Ophiosphaerella aquaticus,Populocrescentia forlicesenensis and Vagicola vagans(Phaeosphaeriaceae),Elongatopedicellata lignicola,Roussoella magnatum and Roussoella angustior(Roussoellaceae)and Shrungabeeja longiappendiculata(Tetraploasphaeriaceae).The new combinations Pseudomassariosphaeria grandispora,Austropleospora archidendri,Pseudopithomyces chartarum,Pseudopithomyces maydicus,Pseudopithomyces sacchari,Vagicola vagans,Punctulariopsis cremeoalbida and Punctulariopsis efibulata Dothideomycetes.The new genera Dictyosporella(Annulatascaceae),and Tinhaudeus(Halosphaeriaceae)are introduced in Sordariomycetes(Ascomycota)while Dictyosporella aquatica(Annulatascaceae),Chaetosphaeria rivularia(Chaetosphaeriaceae),Beauveria gryllotalpidicola and Beauveria loeiensis(Cordycipitaceae),Seimatosporium sorbi and Seimatosporium pseudorosarum(Discosiaceae),Colletotrichum aciculare,Colletotrichum fusiforme and Colletotrichum hymenocallidicola(Glomerellaceae),Tinhaudeus formosanus(Halosphaeriaceae),Pestalotiopsis subshorea and Pestalotiopsis dracaenea(Pestalotiopsiceae),Phaeoacremonium tectonae(Togniniaceae),Cytospora parasitica and Cytospora tanaitica(Valsaceae),Annulohypoxylon palmicola,Biscogniauxia effusae and Nemania fusoideis(Xylariaceae)are introduced as novel species to order Sordariomycetes.The newly described species of Eurotiomycetes are Mycocalicium hyaloparvicellulum(Mycocaliciaceae).Acarospora septentrionalis and Acarospora castaneocarpa(Acarosporaceae),Chapsa multicarpa and Fissurina carassensis(Graphidaceae),Sticta fuscotomentosa and Sticta subfilicinella(Lobariaceae)are newly introduced in class Lecanoromycetes.In class Pezizomycetes,Helvella pseudolacunosa and Helvella rugosa(Helvellaceae)are introduced as new species.The new families,Dendrominiaceae and Neoantrodiellaceae(Basidiomycota)are introduced together with a new genus Neoantrodiella(Neoantrodiellaceae),here based on both morphology coupled with molecular data.In the class Agaricomycetes,Agaricus pseudolangei,Agaricus haematinus,Agaricus atrodiscus and Agaricus exilissimus(Agaricaceae),Amanita melleialba,Amanita pseudosychnopyramis and Amanita subparvipantherina(Amanitaceae),Entoloma calabrum,Cora barbulata,Dictyonema gomezianum and Inocybe granulosa(Inocybaceae),Xerocomellus sarnarii(Boletaceae),Cantharellus eucalyptorum,Cantharellus nigrescens,Cantharellus tricolor and Cantharellus variabilicolor(Cantharellaceae),Cortinarius alboamarescens,Cortinarius brunneoalbus,Cortinarius ochroamarus,Cortinarius putorius and Cortinarius seidlii(Cortinariaceae),Hymenochaete micropora and Hymenochaete subporioides(Hymenochaetaceae),Xylodon ramicida(Schizoporaceae),Colospora andalasii(Polyporaceae),Russula guangxiensis and Russula hakkae(Russulaceae),Tremella dirinariae,Tremella graphidis and Tremella pyrenulae(Tremellaceae)are introduced.Four new combinations Neoantrodiella gypsea,Neoantrodiella thujae(Neoantrodiellaceae),Punctulariopsis cremeoalbida,Punctulariopsis efibulata(Punctulariaceae)are also introduced here for the division Basidiomycota.Furthermore Absidia caatinguensis,Absidia koreana and Gongronella koreana(Cunninghamellaceae),Mortierella pisiformis and Mortierella formosana(Mortierellaceae)are newly introduced in the Zygomycota,while Neocallimastix cameroonii and Piromyces irregularis(Neocallimastigaceae)are introduced in the Neocallimastigomycota.Reference specimens or changes in classification and notes are provided for Alternaria ethzedia,Cucurbitaria ephedricola,Austropleospora,Austropleospora archidendri,Byssosphaeria rhodomphala,Lophiostoma caulium,Pseudopithomyces maydicus,Massariosphaeria,Neomassariosphaeria and Pestalotiopsis montellica. | Hiran A.Ariyawansa Kevin D.Hyde Subashini C.Jayasiri Bart Buyck K.W.Thilini Chethana Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Ruvishika S.Jayawardena Robert Lücking Masoomeh Ghobad-Nejhad Tuula Niskanen Kasun M.Thambugala Kerstin Voigt Rui Lin Zhao Guo-Jie Li Mingkwan Doilom Saranyaphat Boonmee Zhu L.Yang Qing Cai Yang-Yang Cui Ali H.Bahkali Jie Chen Bao Kai Cui Jia Jia Chen Monika C.Dayarathne Asha J.Dissanayake Anusha H.Ekanayaka Akira Hashimoto Sinang Hongsanan E.B.Gareth Jones Ellen Larsson Wen Jing Li Qi-Rui Li Jian Kui Liu Zong Long Luo Sajeewa S.N.Maharachchikumbura Ausana Mapook Eric H.C.McKenzie Chada Norphanphoun Sirinapa Konta Ka Lai Pang Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Umpava Pinruan Emile Randrianjohany Chonticha Singtripop Kazuaki Tanaka Cheng Ming Tian Saowaluck Tibpromma Mohamed A.Abdel-Wahab Dhanushka N.Wanasinghe Nalin N.Wijayawardene Jin-Feng Zhang Huang Zhang Faten A.Abdel-Aziz Mats Wedin Martin Westberg Joseph F.Ammirati Timur S.Bulgakov Diogo X.Lima Tony M.Callaghan Philipp Callac Cheng-Hao Chang Luis F.Coca Manuela Dal-Forno Veronika Dollhofer Kateřina Fliegerová Katrin Greiner Gareth W.Griffith Hsiao-Man Ho Valerie Hofstetter Rajesh Jeewon Ji Chuan Kang Ting-Chi Wen Paul M.Kirk Ilkka Kytövuori James D.Lawrey Jia Xing Hong Li Zou Yi Liu Xing Zhong Liu Kare Liimatainen H.Thorsten Lumbsch Misato Matsumura Bibiana Moncada Salilaporn Nuankaew Sittiporn Parnmen AndréL.C.M.de Azevedo Santiago Sujinda Sommai Yu Song Carlos A.F.de Souza Cristina M.de Souza-Motta Hong Yan Su Satinee Suetrong Yong Wang Syuan-Fong Wei Ting Chi Wen Hai Sheng Yuan Li Wei Zhou Martina Réblová Jacques Fournier Erio Camporesi J.Jennifer Luangsa-ard Kanoksri Tasanathai Artit Khonsanit Donnaya Thanakitpipattana Sayanh Somrithipol Paul Diederich Ana M.Millanes Ralph S.Common Marc Stadler Ji Ye Yan XingHong Li Hye Won Lee Thi T.T.Nguyen Hyang Burm Lee Eliseo Battistin Orlando Marsico Alfredo Vizzini Jordi Vila Enrico Ercole Ursula Eberhardt Giampaolo Simonini Hua-An Wen Xin-Hua Chen Otto Miettinen Viacheslav Spirin Hernawati | 2015 | Fungal Diversity2015，，6： | 2 |
| 17 | Profiling human gut bacterial metabolism and its kinetics using [U‐<sup>13</sup>C]glucose and NMR显示文摘 | Albert A.de Graaf AnnetMaathuis Pieterde Waard Nicolaas E. P.Deutz CorDijkema Willem M.de Vos KoenVenema | 2010 | NMR Biomed2010，，1： | 2 |
| 18 | Activating the ZPD: Mutual Scaffolding in L2 Peer Revision显示文摘 | María C. M.De Guerrero Olga S.Villamil | 2002 | The Modern Language Journal2002，，1： | 2 |
| 19 | 重症肌无力致病性自身抗体的人工构建显示文摘目的构建一株具有致病性的人抗乙酰胆碱受体(AchR)自身抗体,用于诱导猴实验性自身免疫性重症肌无力(EAMG)模型。方法将分离自重症肌无力(MG)患者胸腺的抗AchR抗体的抗原结合片段(Fab637)克隆至含有免疫球蛋白(IgG1)基因的载体pIgG1,构建能在哺乳类细胞表达完整IgG1类抗AchR抗体(IgG1-637)的重组表达载体(pIgG1-637),将pIgG1-637转染CHO-k1细胞系,筛选出稳定表达IgG1-637的细胞株扩大培养。将纯化的IgG1-637注射5只恒河猴,通过检查猴肌肉收缩强度和复合肌肉运动电位(CMAP),判定猴EAMG发病情况,从而鉴定IgG1-637的致病性。结果20μmol/L MSX(L-methionine sulfoxmine)选择的CHO-k1细胞表达最高水平的抗体IgG1-637,表达产量为17.4μg/ml。3只猴(分别接受1.7、1.7和5.0mg/kg的注射剂量)在注射IgG1-637后1周内出现肌肉收缩无力临床症状,并表现出CMAP减弱现象。结论由Fab637构建的完整的IgG1-637是一株致病性抗AchR抗体,可在猴诱导出EAMG。 | 杨康鹃 孟繁平 魏晶 李强 李红花 李英信 M.Stassen M.de Baets | 2006 | 中华微生物学和免疫学杂志2006，26，8： | 2 |
| 20 | Simulations of helicopter ditching using smoothed particle hydrodynamics显示文摘The present work has been performed in the context of the European H2020 project increased SAfety and Robust certification for ditching of Aircrafts and Helicopters(SARAH)dedicated to improving the safety during aircraft ditching,together with a better understanding of the physics involved during those crucial events.Both numerical and experimental aspects are explored during this project.The present study focuses on the application of the smoothed particle hydrodynamics(SPH)method to the simulation of helicopter ditching,as this method has proved to be particularly adapted to free surface impact cases.Simulations are performed for three different impact configurations,for which the numerical solutions are compared with the experimental results(forces and kinematics)obtained at the wave basin of Ecole Centrale Nantes on a mock-up shape provided by Airbus Helicopters.Elements of sensitivity analysis are also provided when needed,to assess the role of some parameters involved in the helicopter behavior and the fluid pressure forces exerted during the impact. | G.Oger A.Vergnaud B.Bouscasse J.Ohana M.Abu Zarim M.De Leffe A.Bannier L.Chiron Y.Jus M.Gamier S.Halbout D.Le Touze | 2020 | Journal of Hydrodynamics2020，32，4： | 2 |
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