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| 1 | Converting lateral scanning into axial focusing to speed up three-dimensional microscopy显示文摘In optical microscopy,the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging.Recently,by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens(ETL)to the back focal plane,rapid axial scanning has been achieved.However,mechanical actuation of a mirror limits the axial scanning rate(usually only 10–100 Hz for piezoelectric or voice coil-based actuators),while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging.In an effort to overcome these limitations,we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging.Using a galvanometric mirror,we scan a laser beam laterally in a remote-focusing arm,which is then back-reflected from different heights of a mirror in the image space.We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude,allowing the quantification of rapid vesicular dynamics in three dimensions.We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope,which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution. | Tonmoy Chakraborty Bingying Chen Stephan Daetwyler Bo-Jui Chang Oliver Vanderpoorten Etai Sapoznik Clemens F.Kaminski Tuomas P.J.Knowles Kevin M.Dean Reto Fiolka | 2020 | Light(Science & Applications)2020，9，1： | 4 |
| 2 | Glia and hemichannels: key mediators of perinatal encephalopathy显示文摘Perinatal encephalopathy remains a major cause of disability, such as cerebral palsy. Therapeutic hypothermia is now well established to partially reduce risk of disability in late preterm/term infants. However, new and complementary therapeutic targets are needed to further improve outcomes. There is increasing evidence that glia play a key role in neural damage after hypoxia-ischemia and infection/inflammation. In this review, we discuss the role of astrocytic gap junction(connexin) hemichannels in the spread of neural injury after hypoxia-ischemia and/or infection/inflammation. Potential mechanisms of hemichannel mediated injury likely involve impaired intracellular calcium handling, loss of blood-brain barrier integrity and release of adenosine triphosphate(ATP) resulting in over-activation of purinergic receptors. We propose the hypothesis that inflammation-induced opening of connexin hemichannels is a key regulating event that initiates a vicious cycle of excessive ATP release, which in turn propagates activation of purinergic receptors on microglia and astrocytes. This suggests that developing new neuroprotective strategies for preterm infants will benefit from a detailed understanding of glial and connexin hemichannel responses. | Robert Galinsky Joanne O.Davidson Justin M.Dean Colin R.Green Laura Bennet Alistair J.Gunn | 2018 | Neural Regeneration Research2018，13，2： | 1 |
| 3 | Measuring Vertical Specialization:The Case of China显示文摘 | Judith M.Dean K.C.Fung Zhi Wang | | 0，，04： | 1 |
| 4 | Flexural properties of fiber‐reinforced polypropylene composites with and without a transcrystalline layer显示文摘 | David M.Dean Alexander A.Marchione LudwigRebenfeld Richard A.Register | 1999 | Polym Adv Technol1999，，11： | 1 |
| 5 | The etiology and prevention of functional instability of the foot显示文摘 | Freeman M M.Dean I.Hanham | | 0，，04： | 1 |
| 6 | Does trade liberalization harm the environment? A new test显示文摘 | Judith M.Dean | 2003 | Canadian Journal of Economics/Revue canadienne d’économique2003，，4： | 1 |
| 7 | 中国外贸日益“清洁”显示文摘研究发现,随着中国进出口贸易的增长,所有工业部门的污染强度到2004年时都已有所降低。很多行业的单位排放量不断降低。其中,中国出口产业正向相对清洁的部门转变。办公用品、计算机与通信器材的出口近年来增长最为迅速,并取代从前的纺织与服装加工业,在出口中占据了最大部分。 | 朱迪思·狄恩(Judith M.Dean) | 2008 | 财经2008，0，11： | 0 |
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