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| 1 | The intragastrointestinal fate of paclitaxel-loaded micelles:Implications on oral drug delivery显示文摘The goal of the present study is to elucidate the intragastrointestinal fate of micellar delivery systems by monitoring fluorescently labeled different micelles and the model drug paclitaxel(PTX).Both in vitro and ex vivo leakage studies showed fast PTX release in fluids while micelles remained intact,except in fedstate simulated intestinal fluid and fasted-state pig intestinal fluid,thus referring to the intact absorption of micelles and PTX leakage in the gastrointestinal tract with D-a-tocopherol polyethylene glycol 1000 succinate(TPGS)micelles showing higher stability than other micelles.All groups of micelles were absorbed intact in Caco-2 and Caco-2/HT29-MTX cell models and the absorption of TPGS micelles was found to be higher than other micelles.The transport of the micelles across Caco-2/Raji(1.6%-3.5%),Caco-2(0.8%-1%),and Caco-2/HT29-MTX(0.58%-1%)cell monolayers further verified the absorption of micelles and their subsequent transport;however,more TPGS micelles transported across cell monolayers than other groups.Moreover,the histological examination also confirmed that micelles ente red the enterocytes and were transported to basolateral tissues and TPGS showed the stronger ability of penetration than other groups.Thus,these results are succinctly presenting the absorption of intact micelles in GIT confirmed by imaging evidence with prior leakage of the drug,upta ke by enterocytes and the transport of micelles that survive the digestion by enterocytes and mainly by microfold cells in material nature dependent way with TPGS showing better results than other groups.In conclusion,these results identify the mechanism by which the gastrointestinal tract processes micelles and point to the likely use of this approach in the design of micelles-based therapies. | Iqbal Zoya Haisheng He Luting Wang Jianping Qi Yi Lu Wei Wu | 2021 | Chinese Chemical Letters2021，32，4： | 3 |
| 2 | Activity of Recombinant Human Interleukin-15 against Tumor Recurrence and Metastasis in Mice显示文摘Transplantable experimental tumor models were constructed to study the activities of recombinant human interleukin-15 (rhIL-15) against tumor recurrence and metastasis. The results showed that tumor nodule formation was retarded and tumor growth was inhibited in the subcutaneous tumor model of LA795 lung adenocarcinoma after treatment with rhIL-15, and the survival rate of T739 tumor-bearing mice treated with rhIL-15 was much higher than that of mice treated with either saline or with the same dose of rhIL-2. This indicats that rhIL-15 had better antitumor effect than rhIL-2 at the same dose level. In some rhIL-15 treated mice, the tumor cells inoculated subcutaneously were eradicated and there was no tumor formation even 138 days after tumor cell inoculation. The tumor-free mice were rechallenged with live tumor cells and no tumor reoccurred in the following two months in all of these mice, indicating that long-lasting antitumor systemic immunity developed. It was also shown that tumor recurrence and metastasis were inhibited markedly after treatment with rhIL-15, but not with the same dose of rhIL-2, in both subcutaneously and intravenously disseminated tumor models of LA795 lung adenocarcinoma. Simultaneously, the CTL and NK cell activities of the splenocytes obtained from tumor-bearing mice that had been treated with either rhIL-15 or rhIL-2 were both markedly enhanced. However, the enhancement of CTL and NK cell activities was more significant in rhIL-15 treated mice than that in rhIL-2 treated mice. This suggests that the anti-tumor effect of rhIL-15 in vivo was achieved by enhancing the CTL and NK cell activities in tumor immune response. | Feng Tang Luting Zhao Yan Jiang Denian Ba Lianxian Cui Wei He | 2008 | Cellular & Molecular Immunology2008，5，3： | 1 |
| 3 | High performance streaked X-ray spectrometer for research of laser-produced plasmas显示文摘In order to mitigate the effect of the strong electromagnetic pulse, which produces laser-plasma interactions, we designed a new streaked X-ray spectrometer (SXS) enclosed within a well-sealed, electromag- netic interference-free cavity. The SXS can cover a wide selection of spectral windows using interchangeable Bragg crystals and by appropriate adjustment of the Bragg angle. The SXS has been used to observe time-re- solved X-ray spectrum in the 2.5 keV to 3.5 keV photon-energy range, resulting in a demonstrated spectral res- olution of about 13 at 2960 eV with a time resolution of 10 ps. The observed time and spectral resolutions dem- onstrate the applicability of the SXS for studies of laser-produced plasmas. | Guo Luting Wei Minxi Hu Xin Deng Keli Chen Tao Deng Bo Zhang Lu Zhao Yang Li Jin Xiao Shaoqiu Liu Shenye | 2016 | 强激光与粒子束2016，28，2： | 0 |
| 4 | Waterborne sub-lethal exposure to perfluorobutanesulfonate causes intestinal dysbiosis in tadpoles of Lithobates catesbeianus显示文摘Perfluorobutanesulfonate (PFBS) is a ubiquitous pollutant in the aquatic environment, but its toxic effects andmechanisms on amphibian species remain largely unknown. In the present study, tadpoles (Lithobates catesbeianus) were exposed to various concentrations of PFBS (0, 1, 3, 10, and 30 μg/L) for 14 days, with the goal ofunveiling the impairment of intestinal health. Histopathological examination showed that sub-lethal exposure oftadpoles to PFBS at concentrations as low as 3 μg/L could result in the injury of intestinal structures. In a clearconcentration-dependent manner, the expressions of epithelial barrier components (i.e., Claudin 1 gene and tightjunction protein 2) were significantly decreased in PFBS-exposed intestines, while the intestinal content oflipopolysaccharide (LPS) and transcriptions of downstream responsive genes (e.g., TLR4, MyD88, and NF-κB) wereconcurrently significantly increased by exposure to 3, 10, and 30 μg/L of PFBS. As a consequence, the number ofeosinophils and expression of pro-inflammatory cytokines (e.g., IL-1β and TNF-α) were increased therein.Furthermore, PFBS exposure induced oxidative stress in intestinal tissues by increasing the level of reactive oxygen species (ROS) and suppressing antioxidant capacity. The transcriptional levels of CytoC and Bax genes aswell as activities of caspase 9 and caspase 3 enzymes were remarkably increased, while the transcript abundanceof Bcl-2 was down-regulated significantly after PFBS exposure, thereby favoring apoptosis in tadpole intestines.PFBS sub-lethal exposure also drove the composition of intestinal microbiota to a dysbiosis status. Correlationanalysis further revealed that the relative abundance of members of the genus Bosea was positively related withthe contents of LPS and IL-1β. Overall, the present study provides the first evidence for pronounced impacts ofPFBS on amphibian intestinal ecology, highlighting the susceptibility of tadpoles to the environmental risks ofPFBS. | Yilin Shu Luting Wei Huiling Jiang Wenchao Wang Huijuan Zhang Lizhu Tang Jun He Kanghua Jiang Hailong Wu Lianguo Chen | 2022 | Water Biology and Security2022，1，4： | 0 |
| 5 | Probing 10μK stability and residual drifts in the cross-polarized dual-mode stabilization of single-crystal ultrahigh-Q optical resonators显示文摘The thermal stability of monolithic optical microresonators is essential for many mesoscopic photonic applications such as ultrastable laser oscillators,photonic microwave clocks,and precision navigation and sensing.Their fundamental performance is largely bounded by thermal instability.Sensitive thermal monitoring can be achieved by utilizing cross-polarized dual-mode beat frequency metrology,determined by the polarization-dependent thermorefractivity of a single-crystal microresonator,wherein the heterodyne radio-frequency beat pins down the optical mode volume temperature for precision stabilization.Here,we investigate the correlation between the dualmode beat frequency and the resonator temperature with time and the associated spectral noise of the dual-mode beat frequency in a single-crystal ultrahigh-Q MgF_(2) resonator to illustrate that dual-mode frequency metrology can potentially be utilized for resonator temperature stabilization reaching the fundamental thermal noise limit in a realistic system.We show a resonator long-term temperature stability of 8.53μK after stabilization and unveil various sources that hinder the stability from reaching sub-μK in the current system,an important step towards compact precision navigation,sensing,and frequency reference architectures. | Jinkang Lim Wei Liang Anatoliy A.Savchenkov Andrey B.Matsko Lute Maleki Chee Wei Wong | 2019 | Light(Science & Applications)2019，8，1： | 0 |
| 6 | The long-circulating effect of pegylated nanoparticles revisited via simultaneous monitoring of both the drug payloads and nanocarriers显示文摘The long-circulating effect is revisited by simultaneous monitoring of the drug payloads and nanocarriers following intravenous administration of doxorubicin(DOX)-loaded methoxy polyethylene glycol-polycaprolactone(mPEG-PCL) nanoparticles. Comparison of the kinetic profiles of both DOX and nanocarriers verifies the long-circulating effect, though of limited degree, as a result of pegylation. The nanocarrier profiles display fast clearance from the blood despite dense PEG decoration;DOX is cleared faster than the nanocarriers. The nanocarriers circulate longer than DOX in the blood, suggesting possible leakage of DOX from the nanocarriers. Hepatic accumulation is the highest among all organs and tissues investigated, which however is reversely proportionate to blood circulation time. Pegylation and reduction in particle size prove to extend circulation of drug nanocarriers in the blood with simultaneous decrease in uptake by various organs of the mononuclear phagocytic system. It is concluded that the long-circulating effect of mPEG-PCL nanoparticles is reconfirmed by monitoring of either DOX or the nanocarriers, but the faster clearance of DOX suggests possible leakage of a fraction of the payloads. The findings of this study are of potential translational significance in design of nanocarriers towards optimization of both therapeutic and toxic effects. | Wufa Fan Haixia Peng Zhou Yu Luting Wang Haisheng He Yuhua Ma Jianping Qi Yi Lu Wei Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 0 |
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