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| 1 | 利用重组反转录病毒表达马立克氏病病毒Meq基因显示文摘为研究马立克氏病病毒(MDV)Meq蛋白的生物学功能,应用磷酸钙与MDVMeq基因重组的反转录病毒转染载体质粒RCAS-Meq的DNA,转染于鸡胚成纤维细胞系DF1。经间接免疫荧光技术研究表明,转染后第3天即开始有少量DF1细胞在细胞核表达Meq蛋白,以后阳性细胞增多,到转染后第7天阳性细胞最多,之后荧光即衰减;通过酶联免疫吸附试验检测,发现细胞培养上清中游离病毒在转染后第5天即可检出,直至第7天仍维持最高水平,第9天开始下降。用细胞培养上清感染DF1细胞单层,于感染后第2天即可检出Meq蛋白阳性细胞,之后阳性细胞逐渐增多,到第4~5天达高峰,之后逐渐衰减;培养上清中的游离病毒在感染后第4天即可被检出,第5~7天达最高值。研究结果表明:(1)磷酸钙法能很好地将重组质粒RCAS-MeqDNA转染到DF1细胞中,而对细胞的损伤很小;(2)转染后细胞释放出的重组病毒能感染DF1细胞并稳定地表达Meq蛋白。表明Meq基因在构建的重组病毒基因组中的存在及其转录表达都是稳定的。这为在体内和体外研究MDVMeq基因的生物学功能提供了一种非常有效的方法和途径;(3)Meq蛋白仅分布于细胞核(包括核质和核仁)。这一结果证实了? | 韦平 Luck F.Lee | 1999 | 中国兽医学报1999，19，2： | 7 |
| 2 | A terrestrial observatory approach to the integrated investigation of the effects of deforestation on water,energy,and matter fluxes显示文摘Integrated observation platforms have been set up to investigate consequences of global change within a terrestrial network of observatories(TERENO)in Germany.The aim of TERENO is to foster the understanding of water,energy,and matter fluxes in terrestrial systems,as well as their biological and physical drivers.Part of the Lower Rhine Valley-Eifel observatory of TERENO is located within the Eifel National Park.Recently,the National Park forest management started to promote the natural regeneration of near-natural beech forest by removing a significant proportion of the spruce forest that was established for timber production after World War II.Within this context,the effects of such a disturbance on forest ecosystem functioning are currently investigated in a deforestation experiment in the Wüstebach catchment,which is one of the key experimental research sites within the Lower Rhine Valley-Eifel observatory.Here,we present the integrated observation system of the Wüstebach test site to exemplarily demonstrate the terrestrial observatory concept of TERENO that allows for a detailed monitoring of changes in hydrological and biogeochemical states and fluxes triggered by environmental disturbances.We present the observation platforms and the soil sampling campaign,as well as preliminary results including an analysis of data consistency.We specifically highlight the capability of integrated datasets to enable improved process understanding of the post-deforestation changes in ecosystem functioning. | H. R. BOGENA R BOL N. BORCHARD N. BRuGGEMANN B. DIEKKRtiGER C. DRuE J. GROH N. GOTTSELIG J. A. HUISMAN A. LuCKE A. MISSONG B. NEUWIRTH T. PuTZ M. SCHMIDT M. STOCKINGER W. TAPPE L. WEIHERMuLLER I. WIEKENKAMP H. VEREECKEN | 2015 | Science China Earth Sciences2015，58，1： | 6 |
| 3 | Carbon dioxide insufflation during colonoscopy in deeply sedated patients显示文摘AIM:To compare the impact of carbon dioxide(CO2) and air insufflation on patient tolerance/safety in deeply sedated patients undergoing colonoscopy.METHODS:Patients referred for colonoscopy were randomized to receive either CO2 or air insufflation during the procedure.Both the colonoscopist and patient were blinded to the type of gas used.During the procedure,insertion and withdrawal times,caecal intubationrates,total sedation given and capnography readings were recorded.The level of sedation and magnitude of patient discomfort during the procedure was assessed by a nurse using a visual analogue scale(VAS)(0-3).Patients then graded their level of discomfort and abdominal bloating using a similar VAS.Complications during and after the procedure were recorded.RESULTS:A total of 142 patients were randomized with 72 in the air arm and 70 in the CO2 arm.Mean age between the two study groups were similar.Insertion time to the caecum was quicker in the CO2 group at 7.3 min vs 9.9 min with air(P = 0.0083).The average withdrawal times were not significantly different between the two groups.Caecal intubation rates were 94.4% and 100% in the air and CO2 groups respectively(P = 0.012).The level of discomfort assessed by the nurse was 0.69(air) and 0.39(CO2)(P = 0.0155) and by the patient 0.82(air) and 0.46(CO2)(P = 0.0228).The level of abdominal bloating was 0.97(air) and 0.36(CO2)(P = 0.001).Capnography readings trended to be higher in the CO2 group at the commencement,caecal intubation,and conclusion of the procedure,even though this was not significantly different when compared to readings obtained during air insufflation.There were no complications in both arms.CONCLUSION:CO2 insufflation during colonoscopy is more efficacious than air,allowing quicker and better cecal intubation rates.Abdominal discomfort and bloating were significantly less with CO2 insufflation. | Rajvinder Singh Eu Nice Neo Nazree Nordeen Ganesananthan Shanmuganathan Angelie Ashby Sharon Drummond Garry Nind Elizabeth Murphy Andrew Luck Graeme Tucker William Tam | 2012 | World Journal of Gastroenterology2012，18，25： | 3 |
| 4 | 城市硬质环境中行道树的效益和机遇显示文摘行道树为城市社区提供环境、经济和社会效益。城市环境给行道树施加压力。不透水路面会引起行道树和路面间的冲突。增进树木健康并减少路面破坏的方法已被测试。渗水铺砌材料是改善树木生长的一种全新且有前景的方法。行道树是城市生活必不可少的一个元素,它们可为居住区和商业区提供大量多样的效益,并通过提供环境、经济和社会效益支撑健康的社区。然而,不渗水路面区域的增加会增加城市生态系统和森林的压力。这些压力通常会导致树木根系在可以为其生长提供更有利条件的地方增殖,但却会引起基础设施的破坏以及路面隆起。这种破坏代价昂贵,已经有多种预防措施被试验来维持树木的健康并减少路面破坏。这篇综述针对跨越30年的可证明行道树效益,城市居民传达出的对行道树的看法,树木根系造成的路面破坏的花费以及对预防路面破坏和改善树木生长试验的措施进行了探究。 | Jennifer Mullaney Terry Lucke Stephen J.Trueman 任栩辉 叶彬彬 刘青林 | 2015 | 农业科技与信息(现代园林)2015，12，5： | 3 |
| 5 | 实验室进行混凝土路面砖透水路面的堵塞试验研究显示文摘在全球化城市发展过程中,为促进雨水渗透、减少地表雨水径流量,改善下游承受水体的水质,正越来越多地利用连锁混凝土路面砖透水性路面系统(英文简称:PICPs)。但是,随着时间推移,地表雨水径流中的沉积物会在PICPs中造成堵塞,大大降低PICPs的渗透能力。过去曾对PICPs的堵塞问题进行过大量研究,其中很多研究都采用在实验室的PICP模型,并使用人工合成的雨水,试图重现和预测出现场铺装PICP的实际堵塞过程。试验使用了各种各样的沉积物类型和颗粒尺寸,以及不同的降雨强度,故研究所获得的结果并不相同。本项目是在实验室内,研究不同类型沉积物、不同颗粒尺寸分布、不同试验期限对PICP模型堵塞情况的影响。利用加速半合成雨水的模拟技术,在实验室分别对18个专门建造的PICP模型进行了试验。对以下三种不同类型的沉积物进行了试验:(1)专门配制的硅砂;(2)颗粒尺寸<300μm的实际雨水沉积物;(3)颗粒尺寸<1.18mm的实际雨水沉积物。本研究采用6个不同降雨速率(降雨强度)进行了试验。研究结果普遍显示,硅砂沉积物的堵塞过程与降雨强度成反比。也就是说,较低降雨强度(较长的试验期限)下的PICP的堵塞程度较弱。相反,颗粒尺寸<300μm的实际雨水沉积物,堵塞过程与降雨强度成正比,即:较低降雨强度(较长的试验期限)下的PICP的堵塞程度更严重。对于颗粒尺寸<1.18mm的沉积物,降雨强度对堵塞程度似乎没有影响。对于判断实验室PICP模型堵塞试验结果的可靠性和有效性,以及如何看待这些结果,提出了一种新的见解。 | Terry Lucke Carsten Dierkes 崔玉忠(译) 杜建东(校) | 2017 | 建筑砌块与砌块建筑2017，，4： | 3 |
| 6 | 青藏高原兴措湖腹足类壳体同位素组成特征与环境意义显示文摘通过对青藏高原东部兴措湖腹足类Gyraulussibirica壳体同位素的分析 ,并与器测气象资料的相关研究 ,建立兴措湖同位素记录与对应的降水和气温间的函数关系。对区内 2 0 0年来的温度序列进行定量恢复 ,结果表明 ,青藏高原东部兴措湖地区腹足类生物壳体主要在温季生长 ,它所记录的是温季而不是全年的气候环境信息 ;Gyraulussibirica壳体δ18O指标与温季滑动平均温度明显为正相关系 ,相关系数为 0 .89;δ13C指标与温季的降水量呈正相关系 ,相关系数 0 .71;近 2 0 0年来研究区温度变化有 3个明显的阶段 ,其中 2 0世纪初的低温期温度比目前平均低 3 .7°C。 | 吴敬禄 Andreas Lucke 李世杰 王苏民 | 2003 | 古生物学报2003，42，2： | 3 |
| 7 | An observer-based compensator for distributed delays显示文摘 | Luck R Ray A | | 0，，： | 2 |
| 8 | The function of Stat3 in tumor cells and their microenvironment显示文摘 | Bernd Groner Peter Lucks Corina Borghouts | 2008 | Seminars in Cell and Developmental Biology2008，，4： | 2 |
| 9 | A gradient analysis of urban landscape pattern: a case study from the Phoenix metropolitan region, Arizona, USA显示文摘 | Matthew Luck Jianguo Wu | 2002 | Landscape Ecology2002，，4： | 2 |
| 10 | Spatio - temporal dynamics of attention to color: Evidence from human electrophysiology显示文摘 | Anllo - Vento Luck S J Hillyard S A | 1998 | Human Brain Mapping1998，6，： | 1 |
| 11 | Right middle lobe syndrome in children显示文摘 | Livingston GL Holinger LD Luck SR | 1987 | Int J Pediatr Otorhinolaryngol1987，13，1： | 1 |
| 12 | Experimental verification of a delay compensation algorithm for integrated communication and control systems 显示文摘 | LUCK R RAY A | 1994 | International Journal of Control1994，59，： | 1 |
| 13 | Texture of Ferritic Stainless Steels显示文摘 | Rabbe D Lucke K | 1993 | Material Science and Technology1993，9，4： | 1 |
| 14 | Laboratory methods for the diagnosis of Legionella infections显示文摘 | Maiwald M Helbig JH Luck PC | 1998 | J Mierobiol Methods1998，33，1： | 1 |
| 15 | Single amino-acid substitutions in recombinant bovine prolactin that markedly reduce its mitogenic activity in Nb2 cell cultures显示文摘 | Luck D N Huyer M Gout P W | 1991 | Mol Endocrino1991，15，： | 1 |
| 16 | Stealth corona-core nanoparticles surface modified by polyethylene glycol: influences of the corona PEG chain length and surface density and of the core composition on phagocytic uptake and plasma protein adsorption显示文摘 | Gref R Luck M Quellec P | 2000 | Colloid SurfB Biointerfaces2000，18，34： | 1 |
| 17 | Studies of a vascular-acting photosensitizer,Pd-bacteriopheophorbide (Tookad),in normal canine prostate and spontaneous canine prostate cancer显示文摘 | Huang Z Chen Q Luck D | 2005 | Lasers Surg Med2005，，： | 1 |
| 18 | Occurrence and distribution of sequence types among Legiortella pneumophila strains isolated from patiens in Germany: common features and differences to other regions of the world显示文摘 | BORCHARDT J HELBIG J H LUCK P C | 2008 | Eur J Clin microbial Infect Dis2008，27，1： | 1 |
| 19 | Communicating Open Systems显示文摘 | INVERNO M LUCK M NORIEGA P | 2012 | Artificial Intelligence2012，186，： | 1 |
| 20 | Theory of mechanical damping due to dislocations 显示文摘 | GRANATO A LUCKE K | 1956 | Applied Physics1956，27，： | 1 |
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