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| 1 | On torsional stiff-ness and natural frequency of bellows 显示文摘 | LuCL Wu TX YuJ G | 2004 | Journal of Mechanical Engineering Science2004，218，3： | 1 |
| 2 | Manual versus target-con-trolled in fusion remifentanil administration in Spontaneously breathing Patients显示文摘 | Annelies T M LucL H Martine MD eta1 | 2009 | Anesth Analg2009，108，3： | 1 |
| 3 | Targeting cancer cells with transferrin conjugates containing the nontoxic type 2 ribosome-inactivating proteins Nigrin b or Ebulin 1显示文摘 | Luclá C J Miguel F Raquel M | 2002 | Cancer Letters2002，184，： | 1 |
| 4 | AnetworkofmiRNAsex-pressed in the ovary are regulated by FSH显示文摘 | YaoN LuCL ZhaoJJ etal | 2009 | Front Biosci2009，14，： | 1 |
| 5 | Photolysis of polyesters, Poly (propylene - 1,2 - malent) and poly( propylene - 1,2 - ortho - phthalate) 显示文摘 | Lucl J Rabek J F | 1981 | European Polymer Jouranl1981，17，： | 1 |
| 6 | Effect of temperature on oxygen consumption and ammonia excretion in the Calafia mother-of-pearl oyster,Pinctada mazatlanica(Hardey,1856)显示文摘 | Saucedo E Lucl'a Ocampo Mario Monteforte | 2004 | Aquaculture2004，229，： | 1 |
| 7 | Effect of temperature on oxygen consumption and ammonia excretion in the Calafia mother-of-pearl oyster,Pinctada mazatlanica(Hanley,1856)显示文摘 | Saucedo E Lucl'a Ocampo Mario Monteforte | 2004 | Aquaculture2004，229，： | 1 |
| 8 | Irritablebowelsyndromeinthe21stcentury:perspectivesfrom AsiaorSouth-eastAsia显示文摘 | ChangFY LuCL | 2007 | JGastroenterolHepatol2007，22，1： | 1 |
| 9 | Effect of temperature on oxygenconsumption and ammonia excretioninthe Calafia mother - of - pearloyster , Pinctada mazatlanica ( Hanley,1856) 显示文摘 | SAUCEDOE LUCL’A OCAMPO MARIIO M | 2004 | Aquaculture2004，229，4： | 1 |
| 10 | Anacetylflavonol from Nervilia fordii(Hance)Schltr显示文摘 | ZhouGX LuCL WangHS | | 0，，06： | 1 |
| 11 | Dumbbell-shaped cervical spinal neurilemmoma presenting asneck mass 显示文摘 | Lucl Chen MH Chao TK | 2005 | J Laryngol 0tol2005，119，： | 1 |
| 12 | Delayed gastrointestinaltransit in patients with hepatocellular carcinoma显示文摘 | CHEN CY LUCL CHANG FY | 2002 | J Gas-troenterol Hepatol2002，17，12： | 1 |
| 13 | Identification of the key amino acids of glial cell line-derived neurotrophic factor family receptor alpha1 involved in its biological function显示文摘Glial cell line-derived neurotrophic factor (GDNF) plays a critical role in neurodevelopment and survival of midbrain dopaminergic and spinal motor neurons in vitro and in vivo. The biological actions of GDNF are mediated by a two-receptor complex consisting of a glycosylphosphatidylinositol-linked cell surface molecule, the GDNF family receptor alpha1 (GFRα1), and receptor protein tyrosine kinase Ret. Although structural analysis of GDNF has been extensively examined, less is known about the structural basis of GFRα1 function. In this study, based on evolutionary trace method and relative solvent accessibility prediction of residues, a set of trace residues that are solvent-accessible was selected for site-directed mutagenesis. A series of GFRα1 mutations was made, and PC12 cell lines stably expressing different GFRα1 mutants were generated. According to the survival and differentiation responses of these stable PC12 cells upon GDNF stimulation and the GDNF-GFRα1-Ret interaction assay, residues 152NN153, Arg259, and 316SNS318 in the GFRα1 central region were found to be critical for GFRα1 binding to GDNF and eliciting downstream signal transduction. The single mutation R259A in the GFRα1 molecule simultaneously lost its binding ability to GDNF and Ret. However N152A/N153A or S316A/N317A/S318A mutation in the GFRα1 molecule still retained the ability to bind with Ret. These findings suggest that distinct structural elements in GFRα1 may be involved in binding to GDNF and Ret. | WangLM ZhangQ ZhuW HeC LuCL DingDF ChenZY | 2005 | 第二军医大学学报2005，26，7： | 0 |
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