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| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Inhibitory action of aqueous coffee ground extracts on the corrosion of carbon steel in HCl solution显示文摘 | Vanessa Vasconcelos Torres Roberto Salgado Amado Camila Faia de Sá Tatiana Lopez Fernandez Carlos Alberto da Silva Riehl Alexandre Guedes Torres Eliane D’Elia | 2011 | Corrosion Science2011，，7： | 1 |
| 3 | Periodontal therapy reduces the rate of preterm low birth weight in women with pregnancy - associated gingivitis显示文摘 | Lopez NJ Da SI Ipinza J | 2005 | Periodontol2005，76，11： | 1 |
| 4 | The Shulman syndrome显示文摘 | Shewmake SW lopez DA Mc Glamory JC | 1978 | Arch Dermatol1978，114，： | 1 |
| 5 | Theory - based interventions for contraception 显示文摘 | Lopez LM Tolley EE Grimes DA | 2013 | Coehrane Database Syst Rev2013，8，00： | 1 |
| 6 | Skin patch and vaginal ring versus combined oral contraceptives for contraception 显示文摘 | Lopez LM Grimes DA Gallo MF | 2008 | Cochrane Database Syst Rev2008，23，00： | 1 |
| 7 | Combination injectable contraceptives for contra-ception显示文摘 | Gallo MF Grimes DA Lopez LM | 2008 | Cochrane Database of Systematic Reviews2008，8，00： | 1 |
| 8 | Osteoporosis and oral diseases 显示文摘 | Estrugo DA Gomez VC Lopez LJ | 2013 | Med Clin(Barc)2013，140，4： | 1 |
| 9 | Steriod hormones for contraception in women with sickle cell disease显示文摘 | Manchikanti A Grimes DA Lopez LM | 2007 | Cochrane Database Syst Rev2007，18，00： | 1 |
| 10 | Cochrane systematic reviews of IUD trials: lessons learned 显示文摘 | Grimes DA Lopez LM Manion C | 2007 | Contraception2007，75，6： | 1 |
| 11 | Myoplasmic Ca2+ concentration during exertional rhabdomyolysis显示文摘 | LoPez JR Rojas B Hayes DA | 1995 | Lancet1995，345，8947： | 1 |
| 12 | Non-steroidal anti-inflammatory drugs for heavy bleeding or pain associated with intrauterine-device use显示文摘 | Grimes DA Hubacher D Lopez LM | 2006 | Cochrane Database Syst Rev2006，18，00： | 1 |
| 13 | Proteomic signatures of serum albumin-bound proteins from stroke patients with and without endovascular closure of PFO are significantly different and suggest a novel mechanism for cholesterol efflux显示文摘 | Lopez MF Krastins B Sarracino DA Byram G Vogelsang MS Prakash A | 2015 | Clin Proteomics2015，12，1： | 1 |
| 14 | Physician-initiated courtesy MODS testing for TB and MDR-TB diagnosis and patient management 显示文摘 | Nic Fhogartaigh CJ Vargas-Prada S Huancar: V Lopez S Rodriguez J Moore DA | 2008 | Int J Tuberc Lung Dis2008，12，5： | 1 |
| 15 | Mass spectrometric discovery and selective reaction monitoring (SRM) of putative protein biomarker candidates in first trimester trisomy 21 maternal serum 显示文摘 | Lopez MF Kuppusamy R Sarracino DA | 2010 | J Proteome Res2010，10，4： | 1 |
| 16 | periodontal therapy reduces the rate of preterm low birth weight in women with pregnancy-associated gingivitis显示文摘 | Lopez NJ Da Silva I Ipinza J | 2009 | Periodentol2009，76，11： | 1 |
| 17 | Education for contraceptive use by women after childbirth 显示文摘 | Lopez LM Hiller JE Grimes DA | 2012 | Cochrane Database Syst Rev2012，158，01： | 1 |
| 18 | Cutaneous meningiomas:a clinicopathologic study显示文摘 | Lopez DA | 1974 | Cancer1974，34，2： | 1 |
| 19 | Sino-implant(Ⅱ)-a levonorgestrel-releasing two-rod implant:systematic review of the randomized controlled trials显示文摘 | Steiner MJ Lopez LM Grimes DA | | 0，，03： | 1 |
| 20 | Cadmium Accumulation in Sunflower Plants Influenced by Arbuscular Mycorrhiza显示文摘 | Sara Adrian Lopez de Andrade Adriana Parada Dias da Silveira Renato Atilio Jorge Monica Ferreira de Abreu | 2008 | International Journal of Phytoremediation2008，，1： | 1 |
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