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| 1 | Dual-color deep-tissue three-photon microscopy with a multiband infrared laser显示文摘Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology.Threephoton(3P)microscopy with excitation in the short-wavelength infrared(SWIR)water transparency bands at 1.3 and 1.7μm opens up new opportunities for deep-tissue imaging.However,novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach.Here,we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7μm that has characteristics optimized for 3P microscopy:sub-70 fs duration,1.25 MHz repetition rate,and μJ-range pulse energy.In turn,we achieve simultaneous 3P excitation of green fluorescent protein(GFP)and red fluorescent proteins(mRFP,mCherry,tdTomato)along with third-harmonic generation.We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain,chick embryo spinal cord,and live adult zebrafish brain,with an improved signal-to-background ratio compared to multicolor twophoton imaging.This development opens the way towards multiparametric imaging deep within scattering tissues. | Khmaies Guesmi Lamiae Abdeladim Samuel Tozer Pierre Mahou Takuma Kumamoto Karolis Jurkus Philippe Rigaud Karine Loulier Nicolas Dray Patrick Georges Marc Hanna Jean Livet Willy Supatto Emmanuel Beaurepaire Frédéric Druon | 2018 | Light(Science & Applications)2018，7，1： | 3 |
| 2 | Geochemical monitoring of atmospheric heavy metal pollution: theory application 显示文摘 | Livete E A | 1988 | Advances in Ecological Research1988，18，： | 1 |
| 3 | Ontology, a Mediator for A gent-Based Modeling in Social Science显示文摘 | Livet P Mtiller J-P Phan D | 2010 | Journal of Artificial Societies and Social Simulation2010，，： | 1 |
| 4 | Ophthalmologic signs in children with autism 显示文摘 | Denis D Burillon C Livet MO | 1997 | J Fr Ophtalmol1997，20，2： | 1 |
| 5 | Influence of predeformation on ageing in an Al–Zn–Mg alloy—I. Microstructure evolution and mechanical properties显示文摘 | A. Deschamps F. Livet Y. Bréchet | 1998 | Acta Materialia1998，，1： | 1 |
| 6 | Monazite-like phases containing transuranium elements (neptunium and plutonium)显示文摘 | A. Tabuteau M. Pagès J. Livet C. Musikas | 1988 | Journal of Materials Science Letters1988，，12： | 1 |
| 7 | Effect of soil aeration on nitrogen availability and growth of selected vegetables-preliminary results显示文摘 | Heuberger H Livet J Schnitzler W | 2001 | Acta Horticulturae2001，56，3： | 1 |
| 8 | Neuronal defects in the hindbrain of Hoxa1,Hoxb1 and Hoxb2 mutants reflect regulatory interactions among these Hox genes显示文摘 | Gavalas A Ruhrberg C Livet J | 2003 | Development2003，130，： | 1 |
| 9 | Influence of copper addition on precipitation kinetics and hardening in A1- Zn-Mg alloy 显示文摘 | Deschamps A Brechet Y Livet F | 1999 | Materials Science and Technology1999，15，： | 1 |
| 10 | Influence of copper addition on precipitation kinetics and hardening in Al-Zn-Mg alloy显示文摘 | Deschamps A Breches Y Livet F | 1999 | Materials Science and Technology1999，15，： | 1 |
| 11 | Geoehemicalmonitoring of atmospheric heavy metal pollution: theory application 显示文摘 | LIVETE E A | 1988 | Adv Ecol Res1988，18，： | 1 |
| 12 | Effect of soil aeration on nitrogen availability and growth of selected vegetables-preliminary results显示文摘 | Heuberger H Livet J Schnitzler W | 2001 | Acta Horticulturae2001，563，： | 1 |
| 13 | Influence of prede- formation on ageing in an AI-Zn-Mg aUoy:I, micro- structure evolution and mechanical properties 显示文摘 | Deschamps A Livet F Brechet Y | 1998 | Ac- ta Materialia1998，47，1： | 1 |
| 14 | Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system显示文摘 | Livet J Weissman TA Kang H | 2007 | Nature2007，450，7166： | 1 |
| 15 | Influence of predeformation and ageing in an Al-Zn-Mg alloy-I Microstructure evolution and mechanical properties显示文摘 | DESCHAMPS A LIVET F BRECHET Y | 1999 | Acta Materialia1999，47，1： | 1 |
| 16 | Influence of predeformation on ageing in an AI-Zn-Mg alloy显示文摘 | Deschamps A Livet F Brechet Y | 1999 | Acta Materialia1999，47，1： | 1 |
| 17 | Influence of copper addition on precipitation kinetics and hardening in Al-Zn-Mg alloy显示文摘 | DESCHAMPS A BRECHET Y LIVET F | 1999 | Materials Science and Technology1999，15，9： | 1 |
| 18 | Initial response to percutaneous ablation predicts survival in patients with hepatocellular carcinoma显示文摘 | Sala M LIvet JM Vilana R etal | 2004 | Hepatology2004，40，： | 1 |
| 19 | Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins显示文摘 | Gu C Yoshida Y Livet J | 2005 | Science2005，307，577： | 1 |
| 20 | Hepatocyte growth factor (HGF/SF) is a muscle-derived survival factor for a subpopulation of embryonic motoneurons显示文摘 | Yamamoto Y Livet J Pollock RA | 1997 | Development1997，124，15： | 1 |
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