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| 1 | Relationship between ATPase activity and conjugated polyamines in mito-chondrial membrane from wheat seedling roots under osmotic stress显示文摘The effects of osmotic stress on the ATPase activity, the contents of —SH group and conjugated polyamines in mitochondrial membrane from wheat seedling [Triticum aestivum L. cv. Yumai No.18(drought-tolerant) and cv. Yumai No.9(drought-sensitive)] roots were investigated. The results showed that ATPase activity and —SH group content decreased with polyethylene glycol(PEG) 6000(-0.55 MPa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine(NCC-Spd)/noncovalently conjugated putrescine(NCC-Put) and increase of the covalently conjugated putrescine(CC-Put). Osmotic stress injury to Yangmai No.9 seedlings was alleviated greatly with 1 mmol/L exogenous spermidine(Spd), in concert with marked increases of the ratio of NCC-Spd/NCC-Put, —SH group contents and ATPase activity in mitochondrial membrane. Under osmotic stress, the concomitant treatment of Yumai No.18 seedlings with methylglyoxyl bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosyl methionine decarboxylase(SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase(TGase), caused a significant decrease of the ratio of NCC-Spd / NCC-Put, CC-Put contents, respectively, in concert with the marked decreases of ATPase activity, —SH group content and its tolerance to osmotic stress. All the results above suggested that osmotic stress tolerance of wheat seedlings was associated with the ATPase activity, the contents of —SH group, NCC-Spd and CC-Put in mitochondrial membrane. | LIUHuai-pan LIUJun ZHANGYan-yan LIUYou-liang | 2004 | Journal of Environmental Sciences2004，16，5： | 16 |
| 2 | Effects of Variation in Activities of Starch-Sugar Metabolic Enzymes on Reducing Sugar Accumulation and Processing Quality of Potato Tubers显示文摘The experiment was designed, via storing potato tubers of cv. E-Potato1 and E-Potato3 indifferent temperatures, to explore the variation patterns of reducing sugar (RS) andtotal sugar (TS) contents and enzyme activities that are involved in the pathway ofstarch-sugar metabolism aiming at identifying the main factors that influence the chipcolor. The results showed that low temperature in storage was a main factor thataccelerated the accumulation of RS of the stored tubers and a very significant linearrelationship existed between RS content and chip color index (CCI) of the tubers. Furtheranalysis elucidated that when tubers stored at 4℃, the activities of ADP glucosepyrophosphorylase (AGPase), UDP glucose pyrophosphorylase (UGPase) and sucrose synthase(SuSy) were negatively exponential to the RS content significantly while that of acidinvertase and alkaline invertase was significantly linear to RS content. It suggestedthat these enzymes could play main roles in the cold sweetening of potato tubers throughregulating starch-sugar metabolism. | CHENGShan-han SUZhen-hong XIECong-hua LIUJun | 2004 | Agricultural Sciences in China2004，3，7： | 10 |
| 3 | Advances in engineering RuO_(2) electrocatalysts towards oxygen evolution reaction显示文摘Water electrolysis technology holds the perfect promise of the hydrogen production,yet control of efficiency and rate of water electrolysis greatly relies on the availability of high-performance electrode materials for kinetic-sluggish oxygen evolution reaction(OER).Accordingly,substantial endeavors have been made to explore advanced electrode materials over the past decade.Recently,RuO_(2) and RuO_(2)-based materials have been demonstrated to be promising for OER due to their remarkable electrocatalytic activity and pH-universal application.Herein,the great achievements and progresses of this flourishing spot are comprehensively reviewed,which are started by a general description of OER to understand the reaction mechanism in detail.Subsequently,the key advantages and issues of RuO_(2) towards OER are also introduced,followed by proposing many advanced strategies for further promoting the electrocatalytic OER performance of RuO_(2).Finally,the daunting challenges and future progresses of RuO_(2) electrocatalysts toward practical water oxidation are highlighted,aiming to provide guidance for the fabrication of desirable RuO_(2)-based electrocatalysts toward OER. | Cheng Wang Liujun Jin Hongyuan Shang Hui Xu Yukihide Shiraishi Yukou Du | 2021 | Chinese Chemical Letters2021，32，7： | 6 |
| 4 | The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq显示文摘Coxsackievirus A16(CVA16) is one of major pathogens of hand, foot and mouth disease(HFMD) in children. Long non-coding RNAs(Inc RNAs) have been implicated in various biological processes,but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of Inc RNAs of normal and CVA16 infected rhabdomyosarcoma(RD)cells using RNA-Seq to investigate the functional relevance of Inc RNAs. We showed that a total of 760 Inc RNAs were upregulated and 1210 Inc RNAs were downregulated. Out of these dysregulated Inc RNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were s RNA host Inc RNAs and 0.05% were enhancer. Six dysregulated Inc RNAs were validated by quantitative PCR assays and the secondary structures of these Inc RNAs were projected. Moreover, we conducted a bioinformatics analysis of an Inc RNAs(ENST00000602478) to elucidate the diversity of modification and functions of Inc RNAs. In summary, the current study compared the dysregulated Inc RNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and Inc RNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD. | Yingying Shi Huilin Tu Xiong Chen Yingying Zhang Liujun Chen Zhongchun Liu Jiqun Sheng Song Han Jun Yin Biwen Peng Xiaohua He Wanhong Liu | 2016 | Virologica Sinica2016，31，2： | 4 |
| 5 | Gas phase tricholoethylene removal at low concentration using activated carbon fiber显示文摘The breakthrough adsorption behaviors of gas phase trichloroethylene in a packed bed of activated carbon fibers(ACF) were investigated. The specific surface area of the ACF was 600 m 2/g, 1400 m 2/g and 1600 m 2/g, respectively, and the concentration of trichloroethylene ranged from 270 mg/m 3 to 2700 mg/m 3. Results showed that the capacity of adsorption increased with increasing specific surface area, the relationship between the logarithms of 10% breakthrough time and concentration was approximately linear over the experimental range, the breakthrough time decreased with increasing temperature and humidity. The breakthrough curves at different inlet concentration or different temperature can be predicted by several simple theoretical models with good agreements. | LIUJun HUANGZheng-hong WANGZhan-sheng KANGFei-yu | 2004 | Journal of Environmental Sciences2004，16，1： | 4 |
| 6 | Experimental Study on the Interaction of Fine Water Spray with Liquid Pool Fires显示文摘An experimental study of the interaction of a fine water spray with liquid pool fires was performed. Spatially resolved droplet size, velocity distributions, and volume flux of the fine water spray have been obtained in two types of pressure-atomized fine spray, using LDAIAPV technology. A method to correct for the presence of droplets is presented and used to determine the heat release rate using a cone calorimeter system, the effects of water spray application on the heat release rate for liquid pool fires were studied. It was found that the volume flux of the water spray is an important factor to determine the liquid pool fire behavior. For the current experimental conditions, the flame was enlarged when lower volume flux of water is used, and suppressed significantly when larger volumes of water are used. Two opposing mechanisms compete when water sprays are applied, which is more complex than the halon agents, such as 1301. The combustion enhancement becomes insignificant when water sprays with the proper characteristics such as adequate volume flux are applied to the diffusion flame in a confined space. | Guangxuan LiaoJianghong LiuJun QinBin Yao State Key Laboratory of Fire Science, University of Science and Technology of China, Hefei, Anhui, 230026,P.R.China | 2001 | Journal of Thermal Science2001，10，4： | 3 |
| 7 | Effect of angiotensin converting enzyme inhibitor on the calcium transients and calcium handling proteins in ventricular myocytes from rats with heart failure显示文摘Background Chronic heart failure (CHF) is associated with calcium transients and calcium handling proteins. Angiotensin converting enzyme (ACE) inhibitor has been demonstrated to have beneficial effect on CHF. Yet studies addressed to the relationship between ACE inhibitor and calcium transients in CHF are rare. The aim of this study was to investigate the influence of ACE inhibitor (perindopril) on the contractility and calcium transients and calcium handling proteins in ventricular myocytes from rats with experimental heart failure.Methods Male Wistar rats were randomized to heart failure group treated with perindopril (CHF-T, 3 mg·kg -1 ·d -1 ), heart failure group without treatment (CHF-C) and sham-operated group (PS). Heart failure was induced by abdominal aortic constriction. All groups were further followed up for 12 weeks. Left ventricular myocytes were then isolated. Single cell shortening fraction and [Ca 2+ ]i were simultaneously measured by laser scanning confocal microscope under the field stimulation (1.0 Hz). Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to evaluate the changes of mRNA and protein of Na +-Ca 2+ exchanger (NCX1), sarcoplasmic reticulum Ca 2+ -ATPase (SERCA2) and phospholamban (PLB).Results The fraction of cell shortening (FS%) and [Ca 2+ ] imax (nmol/L) were significantly reduced in group CHF-C compared with group PS (FS%: 7.51±1.15 vs 13.21±1.49;[Ca 2+ ] imax :330.85±50.05 vs 498.16±14.07; both P <0.01), and restored at least partially in CHF-T group. In CHF-C group, the left ventricular mRNA of NCX1 and PLB were significantly upregulated in comparing with PS group (R NCX1/β-Actin : 0.51±0.12 vs 0.19±0.06, P <0.01; R PLB/β-Actin : 0.26±0.12 vs 0.20±0.08, P <0.05), while SERCA2 mRNA was downregulated (0.48±0.10 vs 0.80±0.11, P <0.01). The mRNA levels of NCX1 and SERCA2 in CHF-T group were between the CHF-C and PS group, and the differences of the latter two groups were significant (all P <0.05). In CHF-C and CHF-T groups, the protein expression of NCX1 were 1.141±0.047 and 1.074±0.081 times of that in PS group respectively (both P <0.05), and SERCA2 protein levels were 0.803±0.100 and 0.893±0.084 times of that in PS group respectively (both P <0.05). The protein expression of NCX1 and SERCA2 in the CHF-C and CHF-T groups is significantly different (both P <0.05).ConclusionACE inhibitor could improve cardiac function of failing heart through directly enhancing the contractility of single cardiomyocyte, and these effects are probably mediated by its roles in preventing the deleterious changes of calcium transients and calcium handling proteins in CHF. | WANGLi-chun MAHong HEJian-gui LIAOXin-xue CHENWen-fang LENGXiu-yu MALi MAIWei-yi TAOJun ZENGWu-tao LIUJun DONGYu-gang TANGAn-li FENGChong | 2005 | Chinese Medical Journal2005，，9： | 2 |
| 8 | Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs显示文摘The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100-300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models. | Yongchun Cui Yi Tian Yue Tang Liujun Jia Aili Wu Peng Peng Jianzhong Yang Hong Du Xiaojuan Wang Like Wu | 2013 | Neural Regeneration Research2013，8，16： | 2 |
| 9 | 显示文摘 | TangLiqiang(唐利强) LiuJun(刘军) ChenZhigang(陈志刚)etal | | 稀有金属材料与工程0，37，1： | 1 |
| 10 | PHISHING WEB IMAGE SEGMENTATION BASED ON IMPROVING SPECTRAL CLUSTERING显示文摘This paper proposes a novel phishing web image segmentation algorithm which based on improving spectral clustering.Firstly,we construct a set of points which are composed of spatial location pixels and gray levels from a given image.Secondly,the data is clustered in spectral space of the similar matrix of the set points,in order to avoid the drawbacks of K-means algorithm in the conventional spectral clustering method that is sensitive to initial clustering centroids and convergence to local optimal solution,we introduce the clone operator,Cauthy mutation to enlarge the scale of clustering centers,quantum-inspired evolutionary algorithm to find the global optimal clustering centroids.Compared with phishing web image segmentation based on K-means,experimental results show that the segmentation performance of our method gains much improvement.Moreover,our method can convergence to global optimal solution and is better in accuracy of phishing web segmentation. | Li Yuancheng Zhao Liujun Jiao Runhai | 2011 | Journal of Electronics(China)2011，28，1： | 1 |
| 11 | Fauhdeteetion and diagnosis of permanent-magnetic DC motor based on parameter estimation and neural network显示文摘 | LIU Xiangqun Zhang Hongyue LiuJun | 2000 | IEEE Transactions on lndustrial Eleetronics2000，47，10： | 1 |
| 12 | Morphology-controlled synthesis of magnetites with nanoporous structures and excellent magnetic properties显示文摘 | Zhao Liujun Zhang Hongjie Xing Yah | 2008 | Chemistry of Materials2008，20，19： | 1 |
| 13 | 显示文摘 | LIUJun(刘军) ZHANGLian-Meng(张联盟) HELi(何莉) etal | | WuhanLigongdaxueXuebao0，，： | 1 |
| 14 | Lnc-RP5 Regulates the mi R-129-5p/Notch1/PFV Internal Promoter Axis to Promote the Expression of the Prototype Foamy Virus Transactivator Tas显示文摘Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical symptoms.Long noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral infections.Previously,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected cells.However,little is known about the function of lnc-RP5 during PFV infection.In this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by lnc-RP5.Moreover,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of Tas.Our results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP axis.This work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection. | Shanshan Xu Liujun Chen Yinglian Tang Peipei Yuan Jun Yan Yingcheng Zheng Li Huang Zhi Li Yan Sun Song Han Jun Yin Qin Pan Biwen Peng Xiaohua He Wanhong Liu | 2020 | Virologica Sinica2020，35，1： | 1 |
| 15 | Evaluating genetic diversity and constrneting core collections of Chinese l,entinulaedodes cuhivars using ISSR and SRAP markers显示文摘 | LiuJun WangZbuoren LiChuang | 2015 | Journal of Basic Microbiology2015，55，： | 1 |
| 16 | Enhanced Biohydrogen Production from Sewage Sludge with Alkaline Pretreatment 显示文摘 | Caimu Lin Liujun Xin Yuansong Wei | 2004 | Environ Sci Technol2004，38，： | 1 |
| 17 | 显示文摘 | LiuJun(刘君) PanYi(盘毅) ZhengChunman(郑春满) | 2012 | 材料导报2012，26，2： | 1 |
| 18 | An efficient adventitious shoot regeneration system for ramie (Boehmeria nivea Gaud) using thidiazuron显示文摘 | Wang Bo Peng Dingxiang Liu Liujun | 2007 | Botanical Studies2007，48，： | 1 |
| 19 | On the WTO's Self-protection Rules to Achieve Win-Win显示文摘 | LiLian HouYan LiuJun | 2005 | China Legal Science2005，，6： | 1 |
| 20 | 显示文摘 | LIUJun ZHANGYong-sheng WANGDong-hong etal(刘军 张永生 王冬红 等) | 2006 | 遥感学报2006，10，1： | 1 |
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