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| 1 | 计算机辅助对几种鲟鱼冻精激活液的比较显示文摘首次在我国采用鱼类彩色精子图文分析系统(FSQAS-2000)对中华鲟、史氏鲟、西伯利亚鲟和匙吻鲟经超低温冷冻保存后的精子,在不同激活液条件下,平均曲线运动速度(VCL)、平均直线运动速度(VSL)、平均路径运动速度(VAP)、平均移动角度(MAD)、以及冷冻前后精子活率的变化等多项参数进行了统计学比较研究。研究分析了不同渗透压、pH以及含Mg^(2+)的激活液对这些参数的影响。研究结果表明:几种鲟鱼激活液的渗透压均较低,其中西伯利亚鲟最低,为10 mOsm·kg^(-1);中华鲟和史氏鲟为20 mOsm·kg^(-1);匙吻鲟最高,为30 mOsm·kg^(-1)。不同的渗透压对冷冻精子的VCL、VSL、VAP、以及精子活率均产生显著(P≤0.05)或极显著(P≤0.01)影响。激活液的pH除了对冷冻精子的上述参数产生影响外,最显著的是对精子的运动轨迹,即平均移动角度(MAD°·s^(-1))产生影响(P≤0.01)。pH越低,精子的MAD越小。几种鲟鱼的最适pH分别为西伯利亚鲟pH 7.5,中华鲟、史氏鲟和匙吻鲟pH 8.5。当4种鲟鱼的冷冻精子激活液中加入5 mmol·L^(-1)MgCl_2时,冷冻精子的3种运动速度显著提高(P≤0.01)。综合分析后认为:西伯利亚鲟的冷冻精子激活液应为10 mmol·L^(-1)Tris-HCl+5 mmol·L^(-1)MgCl_2,pH 7.5;中华鲟和史氏鲟的冷冻精子激活液为20 mmol·L^(-1)Tris-HCl+5 mmol·L^(-1)MgCl_2,pH 8.5;匙吻鲟的冷冻精子激活液为30 mmol·L^(-1)Tris-HCl+5 mmol·L^(-1) MgCl_2,pH 8.5。用此激活液对冷冻前后4种鲟鱼精子活力的相关性进行线性回归分析发现,西伯利亚鲟的R^2=0.8296(P<0.01);中华鲟的R^2=0.9860(P<0.01);史氏鲟的R^2= 0.9622(P<0.01);匙吻鲟R^2=0.9477(P<0.01)。说明冷冻前后4种鲟鱼精子的平均活率存在着极显著的线性正相关性。 | 柳凌 OTOMAR Linhart 危起伟 郭峰 张洁明 刘鉴毅 | 2007 | 水产学报2007，31，6： | 14 |
| 2 | Comparison of PET imaging with a 68 Ga-labelled PSMA ligand and 18 F-choline-based PET/CT for the diagnosis of recurrent prostate cancer显示文摘 | Ali Afshar-Oromieh Christian M. Zechmann Anna Malcher Matthias Eder Michael Eisenhut Heinz G. Linhart Tim Holland-Letz Boris A. Hadaschik Frederik L. Giesel Jürgen Debus Uwe Haberkorn | 2014 | European Journal of Nuclear Medicine and Molecular Imaging2014，，1： | 2 |
| 3 | PET imaging with a [ 68 Ga]gallium-labelled PSMA ligand for the diagnosis of prostate cancer: biodistribution in humans and first evaluation of tumour lesions显示文摘 | A. Afshar-Oromieh A. Malcher M. Eder M. Eisenhut H. G. Linhart B. A. Hadaschik T. Holland-Letz F. L. Giesel C. Kratochwil S. Haufe U. Haberkorn C. M. Zechmann | 2013 | European Journal of Nuclear Medicine and Molecular Imaging2013，，4： | 2 |
| 4 | Biologically and chemically optimised composites of carbonated apatite and polyglycolide as bone substitution materials显示文摘 | Linhart W Peters F Lehmann W | 2001 | J Biomed Mater Res2001，54，： | 1 |
| 5 | Recombinant allergens for allergen-specific immunotherapy: 10 years anniversary of im- munotherapy with recombinant allergens显示文摘 | Valenta R Linhart B Swoboda I | 2011 | Allergy2011，66，6： | 1 |
| 6 | Sexually transmitted infec-tions among brothel-based sex workers in Tel-Aviv area, Israel:high prevalence of pharyngeal gonorrhoea 显示文摘 | Linhart Y Shohat T Amitai Z | 2008 | Int J STD AIDS2008，19，10： | 1 |
| 7 | A recombinant hypoallergenic parvalbumin mutant for immunotherapy of IgE-mediated fish allergy显示文摘 | Swoboda I Bugajska-Schretter A Linhart B | 2007 | J Immunol2007，，10： | 1 |
| 8 | Long-term results of the augmented PFNA: a prospective multicenter trial显示文摘 | C. Kammerlander H. Doshi F. Gebhard A. Scola C. Meier W. Linhart M. Garcia-Alonso J. Nistal M. Blauth | 2014 | Archives of Orthopaedic and Trauma Surgery2014，，3： | 1 |
| 9 | Cardiac manifestations in Fabry disease显示文摘 | LINHART A LUBANDA J C PALECEK T | 2001 | J Inherit Metab Dis2001，24，2： | 1 |
| 10 | Pilot study of combined blockade of the renin-angiotensin system in essential hypertensive patients显示文摘 | Azizi M Linhart A Alexander J | 2000 | J Hypertens2000，18，： | 1 |
| 11 | Comparison of eryoballoon and radiofrequency ablation of pulmonary veins in 40 patients with paroxysmal atrial fibrillation: a case-control study 显示文摘 | Linhart M Bellmann B Mittmann-Braun E | 2009 | J Cardiovase Electrophysiol2009，20，： | 1 |
| 12 | Flexible intramedullary nailing for the treatment of unicameral bone cysts in long bones显示文摘 | Roposch A Saraph V Linhart WE | 2000 | J Bone Joint Surg(Am)2000，82，： | 1 |
| 13 | Comparison of cryoballoon and radiofrequency ablation of pulmonary veins in 40 patients with paroxysmal atrial fibrillation:a case-control study显示文摘 | Linhart M Bellmann B Mittmann-Braun E | 2009 | J Cardiovasc Electrophysiol2009，20，12： | 1 |
| 14 | DEFOG:A practical scheme for deciphering families of genes显示文摘 | FUCHS T MALECOVA B LINHART C | 2002 | Genomics2002，80，3： | 1 |
| 15 | Effects of ions on the motility of fresh and demembranated sperm of common carp(Cyprinus carpio)and paddlefish(Polyodon spathula)显示文摘 | LINHART O COSSON J MIMS S D | 2003 | Fish Physiology&Biochemistry2003，，28： | 1 |
| 16 | Genetic studies of tench:induced triploidy triploidy and tetraploidy and first performance data显示文摘 | Flajshans M Linhart O Kvasnicka P | 1993 | Aquaculture1993，113，： | 1 |
| 17 | Recombinant allergens for allergen-specific immunotherapy: 10 years anniversary of immunotherapy with recombinant allergens 显示文摘 | Valenta R Linhart B Swoboda I | 2011 | Allergy2011，66，6： | 1 |
| 18 | Cryopreservation of sperm in common carp Cyprinus carpio: sperm motility and hatching success of embryos显示文摘 | Linhart O Rodina M Cosson J | 2000 | Cryobiology2000，41，： | 1 |
| 19 | Comparison of cryoballoon and radiofrequency ablation of pulmonary veins in 40 patients with paroxysmal atrial fibrillation: a case-control study显示文摘 | LINHART M BELLMANN B MITTMANN B E | 2009 | J Cardiovasc Electrophysiol2009，20，： | 1 |
| 20 | Genetic structure of populations of geographically restricted and widespread species of Astrgalus (Fabaceae)显示文摘 | KARRON J D LINHART Y B CHAULK C A | 1988 | Amer J Bot1988，75，： | 1 |
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