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| 1 | Wheat MADS-box gene TaSEP3-D1 negatively regulates heading date显示文摘The MADS-box gene plays an important role in regulating plant growth and development.In this study,a SEP3-like MADS-box gene TaSEP3-1 was isolated from bread wheat.The expression patterns of the three homoeologs TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1 were similar,and higher expression levels were detected in floral organs and developing kernels.TaSEP3-D1 was located in the nucleus and cytoplasm and possessed transactivation activity in yeast.Homoeolog sequence polymorphism analysis identified four,three,and four haplotypes of TaSEP3-A1,TaSEP3-B1,and TaSEP3-D1,respectively,and the haplotypes of TaSEP3-D1 had larger effects on agronomic traits than those of TaSEP3-A1 and TaSEP3-B1.D1_h4,significantly associated with heading date,plant height,and other yield-related traits,was the favored haplotype of TaSEP3-D1.Transgenic wheat genotypes overexpressing TaSEP3-D1 exhibited delayed heading and reduced plant height,indicating a role in regulating heading date and plant development.These results shed light on the role of TaSEP3-D1 in wheat plant development.The favored haplotype of TaSEP3-D1 can be applied in breeding to improve plant architecture and yield in wheat. | Lei Zhang Hao Zhang Linyi Qiao Lingfeng Miao Dong Yan Pan Liu Guangyao Zhao Jizeng Jia Lifeng Gao | 2021 | The Crop Journal2021，9，5： | 2 |
| 2 | PseudoRandom Bit Generator Based on Non-Stationary LogisticMaps 显示文摘 | Liu Lingfeng Miao Suoxiao Hu Hanping | 2016 | IET Information Security2016，10，2： | 1 |
| 3 | A faster immunofluorescence assay for tracking infection progress of human cytomegalovirus显示文摘Immunofluorescence 试金(IFA ) 是在生物科学和诊所诊断的最经常使用的方法之一,但是它昂贵、费时间。克服这些限制,我们由修改标准 IFA 开发了更快、更划算的 IFA (f-IFA ) ,并且使用了这个方法追踪人的 cytomegalovirus (HCMV ) 的前进在不同房间的感染。我们开发了的 f-IFA 不仅节省时间,而且戏剧性地减少抗体(Ab ) 的数量,它将在诊所诊断便于 IFA 的应用程序。f-IFA 为堵住,为主要的各个的 10 min 孵化和第二等的 Abs 要求仅仅 15 min,由 1 min 列在后面广泛在每孵化以后洗。仅仅冲淡的 Ab 答案的 25 l 在主要、第二等的 Ab 孵化步骤为每 coverslip 被需要。另外,所有步骤在房间温度被执行。这 f-IFA 成功地被使用了跟随 virion 入口(pp65 ) 和病毒的基因(IE1, UL44,和 pp65 ) 的表示以便追踪 HCMV 感染过程的细节。我们发现 0.5%感染 HCMV 的 T98G 房间形成了 multiple-micronuclei (IE1 并且原子核染色) 并且有由 f-IFA 的流的病毒(染色的 pp65 ) ,它不能被传统的 IFA 检测。我们的结果显示 f-IFA 是为调查病毒感染进步的细节的一个敏感、方便、快、划算的方法,特别 HCMV 感染。有更高的敏感和特性的更快、划算的特征暗示 f-IFA 在临床的诊断有潜在的应用。 | Yingliang Duan Lingfeng Miao Hanqing Ye Cuiqing Yangl Bishi Fu Philip H.Schwartz Simon Rayner Elizabeth A.Formnato Min-Hua Luo | 2012 | Acta Biochimica et Biophysica Sinica2012，44，7： | 1 |
| 4 | Impairment of TRPC1-BK complex in diabetic rat coronary artery显示文摘To the Editor:Diabetes mellitus(DM)is a chronic disease that endangers human health and can lead to different complications.[1]Coronary artery,disease is one of the most serious complications in DM patients. | Manqing Sun Lingling Qian Lingfeng Miao Ying Wu Xiaoyu Liu Shipeng Dang Xu Tang Zhenye Zhang Chao Wang Qiang Chai Fu Yi Jianfeng Hao Ruxing Wang | 2022 | Chinese Medical Journal2022，，15： | 0 |
| 5 | Deciphering the evolution and complexity of wheat germplasm from a genomic perspective显示文摘Bread wheat provides an essential fraction of the daily calorific intake for humanity.Due to its huge and complex genome,progress in studying on the wheat genome is substantially trailed behind those of the other two major crops,rice and maize,for at least a decade.With rapid advances in genome assembling and reduced cost of high-throughput sequencing,emerging de novo genome assemblies of wheat and whole-genome sequencing data are leading to a paradigm shift in wheat research.Here,we review recent progress in dissecting the complex genome and germplasm evolution of wheat since the release of the first high-quality wheat genome.New insights have been gained in the evolution of wheat germplasm during domestication and modern breeding progress,genomic variations at multiple scales contributing to the diversity of wheat germplasm,and complex transcriptional and epigenetic regulations of functional genes in polyploid wheat.Genomics databases and bioinformatics tools meeting the urgent needs of wheat ge-nomics research are also summarized.The ever-increasing omics data,along with advanced tools and well-structured databases,are expected to accelerate deciphering the germplasm and gene resources in wheat for future breeding advances. | Zihao Wang Lingfeng Miao Yongming Chen Huiru Peng Zhongfu Ni Qixin Sun Weilong Guo | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
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