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| 1 | Lentiviral-mediated gene transfer into human adipose-derived stem cells:role of NELL1 versus BMP2 in osteogenesis and adipogenesis in vitro显示文摘(NELL1 ) 象 NEL 一样分子 1 是与 craniosynostosis 联系的一个有势力 osteogenic 因素。侵入人体气管粘膜的病菌,大多数通常为基因治疗使用了病毒的向量,有可以限制成骨的几劣势。以前的研究证明了 lentiviruses 能为骨头新生为基因治疗用作理想的向量。在这研究,编码人的 NELL1 和骨头的二 lentiviral 向量(LvNELL1 和 LvBMP2 ) 分别地,形态基因的 protein-2 (BMP2 ) 被构造。在 vitro 的人的脂肪质导出干细胞(hADSCs ) 的增长,成骨,和 adipogenesis 上的 LvNELL1 感染的效果被估计并且与 LvBMP2 的相比。结果显示出感染 LvNELL1 的那 hADSCs 能高效地并且稳定地 overexpress 目标基因。3-(4,5-dimethylthiazol-2-yl )-2,5-diphenyltetrazolium 溴化物试金结果表明了那 LvBMP2,然而并非 LvNELL1,提高了 hADSCs 的增长。对碱的磷酸酶活动和细胞的矿化作用的评价显示 LvNELL1 感染支持了 hADSCs 的 osteogenic 区别,并且效果与 LvBMP2 的是可比较的。实时聚合酶链反应(PCR ) 在 hADSCs 揭示了那 LvNELL1 感染 upregulated OSX 表情然而并非 RUNX2 表示。另外,分析显示出的 adipogenic 标记(类脂化合物微滴, peroxisome 激活 proliferator 受体,和脂蛋白脂肪分解酵素) LvNELL1 能戏剧性地禁止 hADSCs,而是 LvBMP2 的 adipogenic 区别,这没有如此的效果。一起拿,这些调查结果建议在 hADSCs 的调停 lentiviral 的 NELL1 基因转移可以是一条新奇、有希望的途径完成有效、精确的骨头新生。 | Yajing Liu Caiyun Chen Huixia He Dongsheng Wang LingLing E Zhongyu Liu Hongchen Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，10： | 10 |
| 2 | Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation显示文摘 | Li Hongshi Gu Zexu Wu Li'an Xia Liang Zhou Kecheng E Lingling Wang Dongsheng Kou Junping Liu Hongchen | 2014 | Chinese Medical Journal2014，，20： | 4 |
| 3 | Sustained local delivery of insulin for potential improvement of peri-implant bone formation in diabetes显示文摘Dental implantation is an effective standard treatment modality to restore missing teeth and maxillofacial defects. However, in diabetics there is an increased risk for implant failure due to impaired peri-implant osseous healing. Early topical insulin treat- ment was recently shown to normalize diabetic bone healing by rectifying impairments in osteoblastic activities. In this study, insulin/poly(lactic-co-glycolic acid) (PLGA) microspheres were prepared by a double-emulsion solvent evaporation method. Microspheres were then incorporated in fibrin gel to develop a local drug delivery system for diabetic patients requiring im- plant treatment. In vitro release of insulin from fibrin gel loaded with these microspheres was assessed, and sustained prolonged insulin release over 21 days ascertained. To assess the bioactivity of released insulin and determine whether slow release might improve impaired diabetic bone formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP) activity, mineralized nodule formation, and ELISA (enzyme-linked immunosorbent assay) assays were performed. The insulin released from the drug delivery system stimulated cell growth in previously inhibited cells, and ameliorated the impaired bone-forming ability of human MG-63 cells under high glucose conditions. Fibrin gel loaded with insulin/PLGA microspheres shows potential for improving peri-implant bone formation in diabetic patients. | HAN Yong ZHANG XueYan E LingLing WANG DongSheng LIU HongChen | 2012 | Science China(Life Sciences)2012，55，11： | 2 |
| 4 | Seasonal internode development and sugar me-tabolism in sugarcane 显示文摘 | Lingle S E | 1997 | Crop Science1997，37，： | 1 |
| 5 | Sugar metabolism during growth and develop-ment in sugarcane internodes显示文摘 | Lingle S E | 1999 | Crop Science1999，39，： | 1 |
| 6 | Sugar metabolism during groth and development in sugarcane intemodes显示文摘 | LINGLE S E | 1999 | Crop Sci1999，39，： | 1 |
| 7 | Sucrose metabolism related to growth and ripening in sugarcane internodes显示文摘 | Lingle S E Smith R C | 1991 | Crop Science1991，31，： | 1 |
| 8 | Sugar metabolism in developing Kernels of wheat and barley显示文摘 | CHEVALIER P LINGLE S E | 1983 | Crop Sci1983，23，： | 1 |
| 9 | Sucrose metabolism in netted muskmelon fruit during development显示文摘 | Lingle S E Dunlap J R | 1987 | Plant Physiol1987，84，： | 1 |
| 10 | Sugar metabolism during growth and development in sugarcane internodes显示文摘 | Lingle S E | 1999 | Crop Sci1999，，39： | 1 |
| 11 | Sucrose metabolism in the primary culm of sweet sorghum during development显示文摘 | Lingle S E | 1987 | Crop Sci1987，27，： | 1 |
| 12 | Cloning and expression of sucrose synthase-1 cDNA from sugarcne显示文摘 | Lingle S E Dyear J M | 2001 | Journal of Plant physiology2001，158，1： | 1 |
| 13 | Cyclic nucleotides in invertebrates显示文摘 | Lingle CJ Marder E Nathanson JA | 1982 | Handbook of Experimental Pharmacology1982，58，： | 1 |
| 14 | Cloning and expression of sucrose synthase cDNA from sugarcane显示文摘 | Lingle S E Dyer J M | 2001 | Plant Physiol2001，158，： | 1 |
| 15 | Cloning and expression of sucrose synthase-1 cDNA from sugarcane 显示文摘 | Lingle S E Dyer J M | 2001 | Journal of Plant Physiology2001，158，1： | 1 |
| 16 | Sucrose metabolism in netted muskmelon fruit during development显示文摘 | Lingle S E Dunlap J R | 1987 | Plant Physiol1987，84，： | 1 |
| 17 | Sucrose metabolism in netted muskmelon fruit during development 显示文摘 | Lingle S E Dunlap J R | 1987 | Plant Physiol1987，84，： | 1 |
| 18 | Sucrose synthase and natural ripening in sugarcane显示文摘 | LINGLE S E IRVINE J E | 1994 | Crop Sci1994，34，： | 1 |
| 19 | Sugar metabolism during growth and development in sugarcane internodes显示文摘 | LINGLE S E | 1999 | Crop Sci1999，39，： | 1 |
| 20 | Glimepiride induces proliferation and differentiation of rat osteoblasts via the PI3-kinase/Akt pathway显示文摘 | Pan Ma Bin Gu Junli Ma Lingling E Xia Wu Junkai Cao Hongchen Liu | 2010 | Metabolism2010，，3： | 1 |
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