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| 1 | Generation of selectable marker-free transgenic rice resistant to chewing insects using two co-transformation systems显示文摘生产可选没有标记(SMF ) 对咀嚼昆虫抵抗的转基因的米饭,杆菌 thuringiensis cryIA (c) 基因(Bt ) 被使用二个调停 Agrobacterium 的合作转变系统介绍进二个精英装饰用的梨树米饭变化。一个系统与在一 Agrobacterium 紧张的单个微型双胞胎 T-DNA 二进制向量,它由二个分开的 T-DNA 区域组成,当其它包含可选的标记基因时,带 Bt 的, hygromycin 抵抗基因(HPT ) 。另外的系统在二种分开的 Agrobacterium 文化使用二分开的二进制向量,包含单个原生质标志上的 Bt 或 HPT 基因。怀有 Bt 和可选的标记基因的很多独立转基因的米饭线被获得。结果证明 Bt 基因和 HPT 基因的合作转变频率由由二个分开的二进制向量系统(4.52%) 比那使用微型双胞胎 T-DNA 向量系统(29.87%) 是高得多的。然而,从 co 转基因的植物(21.74%) 的后代获得的 SMF 转基因的米饭植物的频率为后者比那为微型双胞胎 T-DNA 向量系统是更低的(50 – 60%) 。ELISA 的数据暗示表示 Bt 蛋白质作为 0.025 – 被测定; 0.103% 总数长叶在转基因的植物的可溶的蛋白质。因此,没有可选的标记基因,转基因的米饭衬里的几精英被选择。在 vitro 并且在 vivo 昆虫生物鉴定的从两个的结果显示 SMF 转基因的米饭被显示对有斑纹的茎 borer 和米饭叶文件夹高度抵抗。而且处于没有使用的任何杀虫药剂的一个自然的地条件,所有转基因的大米植物被发现到别被大米叶文件夹伤害,而野类型严重被损害。 | Hengxiu Yu Quanhong Yao Ling Wang Zhipeng Zhao Zhiyun Gong Shuzhu Tang Qiaoquan Liu Minghong Gu | 2009 | Progress in Natural Science:Materials International2009，19，11： | 2 |
| 2 | Automatic Identification of Clear-Air Echoes Based on Millimeter-wave Cloud Radar Measurements显示文摘Millimeter-wave cloud radar(MMCR)provides the capability of detecting the features of micro particles inside clouds and describing the internal microphysical structure of the clouds.Therefore,MMCR has been widely applied in cloud observations.However,due to the influence of non-meteorological factors such as insects,the cloud observations are often contaminated by non-meteorological echoes in the clear air,known as clear-air echoes.It is of great significance to automatically identify the clear-air echoes in order to extract effective meteorological information from the complex weather background.The characteristics of clear-air echoes are studied here by combining data from four devices:an MMCR,a laser-ceilometer,an L-band radiosonde,and an all-sky camera.In addition,a new algorithm,which includes feature extraction,feature selection,and classification,is proposed to achieve the automatic identification of clear-air echoes.The results show that the recognition algorithm is fairly satisfied in both simple and complex weather conditions.The recognition accuracy can reach up to 95.86%for the simple cases when cloud echoes and clear-air echoes are separate,and 88.38%for the complicated cases when low cloud echoes and clear-air echoes are mixed. | Ling YANG Yun WANG Zhongke WANG Qian YANG Xingang FAN Fa TAO Xiaoqiong ZHEN Zhipeng YANG | 2020 | Advances in Atmospheric Sciences2020，37，8： | 2 |
| 3 | Optimisation of p-doped I~c-Si: H emitter layers in crystalline-amorphous silicon heterojunction solar cells 显示文摘 | Ling Zhipeng Ge Jia Mueller Thomas | 2012 | Energy Procedia2012，15，1： | 1 |
| 4 | Identification of three members of the Bmp family from yellow catfish Pelteobagrus fulvidraco and their transcriptional responses to a high fat diet显示文摘In this study,three members of the Bmp family were cloned and characterized in yellow catfish Pelteobagrus fulvidraco,including Bmp2a,Bmp4 and Bmp9.The predicted amino acid sequences of P.fulvidraco Bmp2a,Bmp4 and Bmp9 showed the characteristic domains of the Bmp family,including an N-terminal signal peptide,Arg-X-XArg site,TGF-β family signature and seven conserved cysteines,indicating that function is likely to have been conserved during evolution.mRNAs of the three Bmp genes had a variable level of expression in tissues.Compared to the control diet,a high fat diet tended to down-regulate the mRNA expression of Bmp2a,Bmp4 and Bmp9 in mesenteric fat,liver and ovary,while it tended to up-regulate their mRNA levels in muscle and kidney.The responses to dietary lipid status and the potential role in lipid metabolism have not previously been reported and reinforces the idea of their multiple functions.Our findings provide the first data about the potential role of the Bmp family in lipid metabolism in teleost. | Zhipeng Tai Shicheng Ling Jie Cheng Xiaoying Tan | 2021 | Aquaculture and Fisheries2021，6，1： | 0 |
| 5 | Terahertz wave avalanche breakdown transistor for high-performance switching显示文摘There is still a lack of high-performance terahertz(THz) modulators with wide operation bandwidth and large modulation depth due to the underlying physics limitation behind existing approaches. Meanwhile, for many applications, simple compact THz modulators working straightforward in the transmission mode are also highly desired. Here, we demonstrate a THz modulator with a maximal transmission-amplitude modulation depth of 99.9%(switching ratio of 1000) based on a commonly used silica-on-silicon structure. Different from those reported graphene or metamaterials enhanced proposals, the device we proposed works within a reversible avalanche breakdown region of silicon that has not been studied yet and has the potential to modulate/switch THz waves efficiently. Further, we proved that the modulation depth exceeds 97% in the frequency range from 0.2 to 1 THz in the experiment. The simplicity and generality of this new type of near-perfect THz modulator will undoubtedly attract lots of attention of researchers in the near future due to its potential to be engineered into integrated devices. | Weijun WANG Liang-Hui Du Jiang Li Pei-Ren Tang Changlin Sun Songlin Chen Jun Wang Zhao-Hui Zhai Zhipeng Gao Ze-Ren Li Jianquan Yao Furi Ling Li-Guo Zhu | 2021 | Photonics Research2021，9，3： | 0 |
| 6 | HDAC3在核心昼夜节律负反馈环中两种不同的作用对生物钟功能至关重要显示文摘组蛋白去乙酰化酶(HDAC)是一类蛋白酶,对染色体的结构修饰和基因表达调控有重要的作用。一般情况下,组蛋白的乙酰化有利于DNA与组蛋白八聚体的解离,核小体结构松弛,从而使各种转录因子和协同转录因子能与DNA结合位点特异性结合,激活基因的转录。 | Guangsen Shi Pancheng Xie Zhipeng Qu Zhihui Zhang Zhen Dong Yang An Lijuan Xing Zhiwei Liu Yingying Dong Guoqiang Xu Ling Yang Yi Liu 徐璎 | 2017 | 科学新闻2017，19，4： | 0 |
| 7 | Innovative insights into extrachromosomal circular DNAs in gynecologic tumors and reproduction显示文摘Originating but free from chromosomal DNA,extrachromosomal circular DNAs(eccDNAs)are organized in circular form and have long been found in unicellular and multicellular eukaryotes.Their biogenesis and function are poorly understood as they are characterized by sequence homology with linear DNA,for which few detection methods are available.Recent advances in high-throughput sequencing technologies have revealed that eccDNAs play crucial roles in tumor formation,evolution,and drug resistance as well as aging,genomic diversity,and other biological processes,bringing it back to the research hotspot.Several mechanisms of eccDNA formation have been proposed,including the breakage-fusion-bridge(BFB)and translocation-deletion-amplification models.Gynecologic tumors and disorders of embryonic and fetal development are major threats to human reproductive health.The roles of eccDNAs in these pathological processes have been partially elucidated since the first discovery of eccDNA in pig sperm and the double minutes in ovarian cancer ascites.The present review summarized the research history,biogenesis,and currently available detection and analytical methods for eccDNAs and clarified their functions in gynecologic tumors and reproduction.We also proposed the application of eccDNAs as drug targets and liquid biopsy markers for prenatal diagnosis and the early detection,prognosis,and treatment of gynecologic tumors.This review lays theoretical foundations for future investigations into the complex regulatory networks of eccDNAs in vital physiological and pathological processes. | Ning Wu Ling Wei Zhipeng Zhu Qiang Liu Kailong Li Fengbiao Mao Jie Qiao Xiaolu Zhao | 2024 | Protein & Cell2024，15，1： | 0 |
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