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| 1 | African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2显示文摘African swine fever virus(ASFV),as a member of the large DNA viruses,may regulate autophagy and apoptosis by inhibiting programmed cell death.However,the function of ASFV proteins has not been fully elucidated,especially the role of autophagy in ASFV infection.One of three Pyrroline-5-carboxylate reductases(PYCR),is primarily involved in conversion of glutamate to proline.Previous studies have shown that depletion of PYCR2 was related to the induction of autophagy.In the present study,we found for the first time that ASFV E199 L protein induced a complete autophagy process in Vero and HEK-293 T cells.Through co-immunoprecipitation coupled with mass spectrometry(Co IP-MS)analysis,we firstly identified that E199 L interact with PYCR2 in vitro.Importantly,our work provides evidence that E199 L down-regulated the expression of PYCR2,resulting in autophagy activation.Overall,our results demonstrate that ASFV E199 L protein induces complete autophagy through interaction with PYCR2 and down-regulate the expression level of PYCR2,which provide a valuable reference for the role of autophagy during ASFV infection and contribute to the functional clues of PYCR2. | Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie | 2021 | Virologica Sinica2021，36，2： | 7 |
| 2 | Impact of serum levels of lipoprotein lipase, hepatic lipase, and endothelial lipase on the progression of coronary artery disease显示文摘Purpose: The purpose of this study was to investigate the relationship between serum levels of lipoprotein lipase(LPL), hepatic lipase(HL), and endothelial lipase(EL) and the progression of coronary artery disease(CAD).Materials and methods: According to the inclusion criteria, exclusion criteria, diagnostic criteria, angiography results, and the random matching scheme, the enrolled patients were divided into the following two groups: the progression-free group(n ? 47) and the progression group(n ? 15). The baseline characteristics and various biochemical parameters were obtained from the medical records and medical history. Serum LPL, HL, and EL levels were detected by ELISA. The correlation between serum LPL, HL, and EL levels and coronary lesions was statistically analyzed with SPSS software.Results: Significant differences were observed in serum levels of HL and EL between the progression-free group and the progression group(HL, 75.5 ? 39.2 ng/mL vs. 125.1 ? 42.1 ng/mL, P < 0.05;EL, 139.2 ? 59.6 pg/mL vs.175.1 ? 40.1 pg/mL, P < 0.05), while the difference in the LPL level was not significant(P > 0.05). Receiver operating characteristic curve(ROC) analysis showed that the area under the curve(AUC) values of LPL, HL, and EL were 0.506(95% CI: 0.369–0.642, P ? 0.9470), 0.792(95% CI: 0.664–0.888, P < 0.0001), and 0.693(95% CI:0.553–0.811, P ? 0.0095), respectively. Additionally, logistic regression analysis showed that the serum level of HL was an independent risk factor for coronary artery lesion progression.Conclusion: Serum levels of EL and HL, but not the serum level of LPL, were positively correlated with the progression of CAD. The serum level of HL was an independent risk factor for the progression of CAD, while the serum level of EL or LPL was not an independent risk factor for the progression of CAD. For the diagnosis of CAD progression, the serum level of HL was better than the serum level of EL or LPL. | Hui Han Daopeng Dai Wencheng Wang Jinzhou Zhu Zhengbin Zhu Lin Lu Ruiyan Zhang | 2019 | Journal of Interventional Medicine2019，2，1： | 6 |
| 3 | Reduction of polyethylenimine-coated iron oxide nanoparticles induced autophagy and cytotoxicity by lactosylation显示文摘Superparamagnetic iron oxide(SPIO)nanoparticles are excellent magnetic resonance contrast agents and surface engineering can expand their applications.When covered with amphiphilic alkyl-polyethyleneimine(PEI),the modified SPIO nanoparticles can be used as MRI visible gene/drug delivery carriers and cell tracking probes.However,the positively charged amines of PEI can also cause cytotoxicity and restricts their further applications.In this study,we used lactose to modify amphiphilic low molecular weight polyethylenimine(C_(12-)PEI_(2K))at different lactosylation degree.It was found that the N-alkyl-PEI-lactobionic acid wrapped SPIO nanocomposites show better cell viability without compromising their labelling efficacy as well as MR imaging capability in RAW 264.7 cells,comparing to the unsubstituted ones.Besides,we found the PEI induced cell autophagy can be reduced via lactose modification,indicating the increased cell viability might rely on down-regulating autophagy.Thus,our findings provide a new approach to overcome the toxicity of PEI wrapped SPIO nanocomposites by lactose modification. | Jiuju Du Wencheng Zhu Li Yang Changqiang Wu Bingbing Lin Jun Wu Rongrong Jin Taipeng Shen Hua Ai | 2016 | Regenerative Biomaterials2016，3，4： | 5 |
| 4 | The nonresonant perturbation theory based field measurement and tuning of a linac accelerating structure显示文摘Assisted by the bead pull technique,nonresonant perturbation theory is applied for measuring and tuning the field of the linac accelerating structure.The method is capable of making non-touching amplitude and phase measurements,real time mismatch feedback and field tuning.Some key considerations of the measurement system and of a C-band traveling-wave structure are discussed,and the bead pull measurement and the tuning of the C-band traveling-wave linac accelerating structure are presented at last. | FANG WenCheng TONG DeChun GU Qiang ZHAO ZhenTang SHENG Xing CHEN LiFang WANG Lin | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，11： | 3 |
| 5 | Radar observations of intense orographic precipitation associated with Typhoon Xangsane (2000)显示文摘 | Cheng Kuyu Lin Wencheng | 2008 | MonWea Rev2008，136，2： | 1 |
| 6 | Facile scalable one-step wet-spinning of surgical sutures with shape memory function and antibacterial activity for wound healing显示文摘Surgical suture is commonly used in clinic due to its action in accelerating the process of wound healing.However,difficultly handling in minimally invasive surgery and bacteria-induced infection usually limit its use in a wide range of applications.Here,we report a facile scalable strategy to fabricate surgical sutures with shape memory function and antibacterial activity for wound healing.Specifically,a shape memory polyurethane(SMPU)with a transition temperature(Ttrans)at 41.3℃was synthesized by adjusting the mole ratio of the hard/soft segment,and then the shape memory surgical sutures containing polyhexamethylene biguanide hydrochloride(PHMB)as a model drug for antibacterial activity were fabricated by a facile scalable one-step wet-spinning approach,in which PHMB was directly dissolved in the coagulation bath that enable its loading into the sutures through the dual diffusion during the phase separation.The prepared sutures were characterized by their morphology,mechanical properties,shape memory,antibacterial activity,as well as biocompatibility before the wound healing capability was tested in a mouse skin suture-wound model.It was demonstrated that the optimized suture is capable of both shape memory function and antibacterial activity,and promote wound healing,suggesting that the facile scalable one-step wet-spinning strategy provides a promising tool to fabricate surgical sutures for wound healing. | Xinghan Chen Pengfei Tan Ya Wen Wencheng Zhou Ying Cen Chao You Lin Tan Meng Tian | 2020 | Chinese Chemical Letters2020，31，6： | 1 |
| 7 | Integrating Textual and Visual Information for Cross-language Image Retrieval显示文摘 | Lin Wencheng Chang Y C Chen H H | 2007 | Infor- mation Processing and Management2007，43，5： | 1 |
| 8 | The effect of spectrum on visual field in road lighting显示文摘 | Lin Yandan Chen Wencheng Chen Dahua | 2004 | Building and Environ ment2004，39，： | 1 |
| 9 | Transla- ting/transliterating named entities for multilingual infimnation ac- cess显示文摘 | Chen ttsin-Hsi Lin Wencheng Yang Changhua | 2006 | Journal of the American Society for Information Science and Technology (Special Issue on Muhilingual Information Sys- tems)2006，57，5： | 1 |
| 10 | Starvation-Sensitized and Oxygenation-Promoted Tumor Sonodynamic Therapy by a Cascade Enzymatic Approach显示文摘The therapeutic outcomes of noninvasive sonodynamic therapy(SDT)are always compromised by tumor hypoxia,as well as inherent protective mechanisms of tumor.Herein,we report a simple cascade enzymatic approach of the concurrent glucose depletion and intratumoral oxygenation for starvation-sensitized and oxygenation-amplified sonodynamic therapy using a dual enzyme and sonosensitizer-loaded nanomedicine designated as GOD/CAT@ZPF-Lips.In particular,glucose oxidase-(GOD-)catalyzed glycolysis would cut off glucose supply within the tumor,resulting in the production of tumor hydrogen peroxide(H_(2)O_(2))while causing tumor cells starvation.The generated H_(2)O_(2)could subsequently be decomposed by catalase(CAT)to generate oxygen,which acts as reactants for the abundant singlet oxygen(^(1 O_(2))production by loaded sonosensitizer hematoporphyrin monomethyl ether(HMME)upon the US irradiation,performing largely elevated therapeutic outcomes of SDT.In the meantime,the severe energy deprivation enabled by GOD-catalyzed glucose depletion would prevent tumor cells from executing protective mechanisms to defend themselves and make the tumor cells sensitized and succumbed to the cytotoxicity of^(1 O_(2)).Eventually,GOD/CAT@ZPF-Lips demonstrate the excellent tumoral therapeutic effect of SDT in vivo without significant side effect through the cascade enzymatic starvation and oxygenation,and encouragingly,the tumor xenografts have been found completely eradicated in around 4 days by the intravenous injection of the nanomedicine without reoccurrence for as long as 20 days. | Wencheng Wu Yinying Pu Han Lin Heliang Yao Jianlin Shi | 2021 | Research2021，，1： | 1 |
| 11 | lntegrating Textual and Visual Information for Cross-language Image Retrieval显示文摘 | Lin Wencheng Chang Yihchen Chen Hsinhsi | 2007 | Information Processing and Management2007，，43： | 1 |
| 12 | Femtosecond laser micro-nano processing for boosting bubble releasing of gas evolution reactions显示文摘Coupling effect of chemical composition and physical structure is a key factor to construct superaerophobic electrodes.Almost all reports about superaerophobic electrodes were aimed at precisely controlling morphology of loaded materials(constructing specific structure)and ignored the due role of substrate.Nevertheless,in this work,by using high precision and controllable femtosecond laser,hierarchical micro-nano structures with superaerophobic properties were constructed on the surface of silicon substrate(fs-Si),and such special super-wettability could be successfully inherited to subsequent self-supporting electrodes through chemical synthesis.Femtosecond laser processing endowed electrodes with high electrochemical surface area,strong physical structure,and remarkable superaerophobic efficacy.As an unconventional processing method,the reconstructed morphology of substrate surface bears the responsibility of superaerophobicity,thus liberating the structural constraints on loaded materials.Since this key of coupling effect is transferred from the loaded materials to substrate,we provided a new general scheme for synthesizing superaerophobic electrodes.The successful introduction of femtosecond laser will open a new idea to synthesize self-supporting electrodes for gas-involving reactions. | Shuai Zhang Lishuang Xu Jie Wu Ying Yang Chengxin Zhang Haiyan Tao Jingquan Lin Licheng Huang Wencheng Fang Keying Shi Xiangting Dong | 2022 | Nano Research2022，15，2： | 0 |
| 13 | Corrigendum to“African swine fever virus protein E199L promotes cell autophagy through the interaction of PYCR2”[Virol Sin 36(2021)196–206]显示文摘Due to our negligence,the original version of this article,published online on April 08,2021,contained a mistake in Fig.1E.The lane ofβ-actin in Western blotting was misused.The correct Fig.1 is given below.We apologize for our oversight when preparing the figure and state that this does not change the scientific conclusions of the article in any way. | Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie | 2023 | Virologica Sinica2023，38，3： | 0 |
| 14 | Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus显示文摘Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles(EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles(SE) were isolated and purified from ALV-J-infected rooster seminal plasma(SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection.More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission. | Liqin Liao Weiguo Chen Xiangyu Zhang Huanmin Zhang Aijun Li Yiming Yan Zi Xie Hongxing Li Wencheng Lin Jingyun Ma Xinheng Zhang Qingmei Xie | 2022 | Virologica Sinica2022，37，2： | 0 |
| 15 | Amplified visualization and function exploration of exosomal protein-specific glycosylation using hybridization chain reaction from non-functional epitope显示文摘Exosomal glycoproteins play significant roles in many physiological and pathological procedures. However, the current methods for studying exosomal glycoproteins have low sensitivity or can affect exosomal biological function. Herein, we developed a proximity dual-tagging strategy using an induced hybridization chain reaction(HCR) from the target’s non-functional epitope for amplified visualization and functional exploration of exosomal protein-specific glycosylation. This strategy leverages dualtagging based on the aptamer with little influence on target function and metabolic glycan labelling, and the rigid product and high sensitivity of HCR. The method improves the signal of visualizing exosomal PD-L1(exo PD-L1) by 7.7-fold compared with the signal without HCR amplification without affecting the natural exo PD-L1/PD-1 interaction. As a result, we verified that the interaction between exo PD-L1 and PD-1 positive cells is positively correlated to the glycosylation level of exo PD-L1. Overall,we have developed a sensitive method with little functional influence to visualize exosomal protein-specific glycosylation in situ,offering a powerful tool for studying the biological implications of exosomal glycoproteins. | Siyin Kang Lin Zhu Wencheng Wang Yinzhu Lu Zhenlong You Chi Zhang Yuanfeng Xu Chaoyong Yang Yanling Song | 2022 | Science China Chemistry2022，65，6： | 0 |
| 16 | Conceptual study and design of A C-band photocathode injector显示文摘Purpose A C-band photocathode injector composed of a 3.6-cell C-band photocathode RF gun and two 1.8-m C-band accelerating structures is proposed.The injector is a low emittance electron source for free electron lasers(FEL)and other compact light sources.Methods The RF structure of the cavities is designed with 2D SUPERFISH simulation.The beam dynamic study in ASTRA helps rectify the 2D RF simulation.To feed the cavities,a design of extracoaxial coupler with RF gun structure is presented.Results With compact focusing solenoids,for 0.25 nC bunch charge,the final energy can reach 6.9 MeV energy and the 95%emittance can be as low as 0.23 mm mrad(95%).Conclusion All the details of RF design and beam dynamics studies are presented in this paper,and the design satisfied the requirements of FEL and other applications. | Wencheng Fang Lin Wang Zhentang Zhao | 2019 | Radiation Detection Technology and Methods2019，3，3： | 0 |
| 17 | Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus显示文摘Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape(Brassica napus).Breeding for Sclerotinia resistance is challenging,as no immune germplasm available in B.napus.It is desirable to develop new breeding strategies.In the present study,hostinduced gene silencing(HIGS),developed based on RNA interference(RNAi),was applied to protect B.napus from S.sclerotiorum infection.Three pathogenicity genes,the endo-polygalacturonase gene(Ss PG1),cellobiohydrolase gene(Ss CBH),and oxaloacetate acetylhydrolase gene(Ss OAH1),were chosen as HIGS targets.Co-incubation of synthesized double-stranded RNAs(ds RNAs)with S.sclerotiorum in liquid medium significantly reduced the transcript levels of the target genes.Application to plant surfaces of ds RNA targeting the three genes conferred effective protection against S.sclerotiorum.Stable transgenic B.napus plants expressing small interfering RNAs with sequence identity to Ss PG1,Ss CBH,and Ss OAH1 were generated.HIGS transgenic B.napus prevented the expression of S.sclerotiorum target genes,slowed pathogenicity-factor accumulation,impeded fungal growth,and suppressed appressorium formation,thereby conferring resistance to S.sclerotiorum.Simultaneous silencing of Ss PG1,Ss CBH,and Ss OAH1 by stable expression of a chimeric hairpin RNAi construct in B.napus led to enhanced protection phenotypes(with disease lesion size reduced by 36.8%–43.7%).We conclude that HIGS of pathogenic-factor genes of S.sclerotiorum is a promising strategy for controlling Sclerotinia rot in oilseed rape. | Jian Wu Shengliang Yin Li Lin Dongxiao Liu Sichao Ren Wenjing Zhang Wencheng Meng Peipei Chen Qinfu Sun Yujie Fang Cunxu Wei Youping Wang | 2022 | The Crop Journal2022，10，3： | 0 |
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