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| 1 | Selection of optimal antisense accessible sites of survivin and its application in treatment of gastric cancer显示文摘AIM: To select the optimal antisense accessible sites of survivin, a highly expressed gene in tumor tissues, in order to explore a novel approach to improve biological therapy of gastric cancer.METHODS: The 20 mer random oligonucleotide library was synthesized, hybridized with in vitro transcribed total survivin cRNA, then digested by RNase H. After primer extension and autoradiography, the antisense accessible sites (AAS) of survivin were selected. Then RNADraw software was used to analyze and choose the AAS with obvious stem-loop structures, according to which the complementary antisense oligonucleotides (AS-ODNs) were synthesized and transferred into survivin highly- expressing gastric cancer cell line MKN-45. Survivin expression was detected by RT-PCR and Western Blotting. Cellular growth activities were assayed by tetrazolium bromide (MTT)colorimetry. Cellular ultrastructure was observed by electronic microscopy, while apoptosis was detected by annexin V-FITC and propidium iodide staining flow cytometry.RESULTS: Thirteen AAS of survivin were selected in vitro.Four AAS with stem-loop structures were chosen, locating at 207-226 bp, 187-206 bp, 126-145 bp and 44-63 bp of survivin cDNA respectively. When compared with nontranfection controls, their corresponding AS-ODNs (AS-ODN1,AS-ODN2, AS-ODN3 and AS-ODN4) could reduce Survivin mRNA levels in MKN-45 cells by 54.3±1.1% (t= 6.12, P<0.01),86.1±1.0% (t= 5.27, P<0.01), 32.2±1.3% (t= 7.34, P<0.01)and 56.2±0.9% (t= 6.45, P<0.01) respectively, while survivin protein levels were decreased by 42.2±2.5% (t = 6.26,P<0.01), 75.4±3.1% (t= 7.11, P<0.01), 28.3±2.0% (t= 6.04,P<0.01) and 45.8±1.2% (t = 6.38, P<0.01) respectively.After transfection with 600 nmol/L AS-ODN1~AS-ODN4 for24 h, cell growth was inhibited by 28.12±1.54% (t= 7.62,P<0.01), 38.42±3.12% (t = 7.75, P<0.01), 21.46±2.63%(t= 5.94, P<0.01) and 32.12±1.77% (t= 6.17, P<0.01)respectively. Partial cancer cells presented the characteristic morphological changes of apoptosis, with apoptotic rates being 19.31±1.16% (t=7.16, P<0.01), 29.24±1.94%(t = 8.15, P<0.01), 11.87±0.68% (t= 6.68, P<0.01) and21.68±2.14% (t = 7.53, P<0.01) respectively.CONCLUSION: The AAS of survivin could be effectively selected in vitro by random oligonucleotide library/RNase H cleavage method combined with computer software analysis, this has important reference values for further studying survivin-targeted therapy strategies for gastric cancer. | Qiang-SongTong Li-DuanZheng Fang-MinChen Fu-QingZeng LiangWang Ji-HuaDong Gong-ChengLu | 2005 | World Journal of Gastroenterology2005，11，5： | 27 |
| 2 | Dendritic Cells as Vectors for Immunotherapy of Tumor and Its Application for Gastric Cancer Therapy显示文摘Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells (APCs) with the ability to stimulate na(i)ve resting T cells and initiate primary immune responses. DCs are poised to capture antigen (Ag),migrate to draining lymphoid organs, and, after a process of maturation, select Ag-specific lymphocytes to which they present the processed Ag, thereby inducing immune responses. Numerous studies indicated that immunotherapies utilizing DC-presenting tumor-associated antigens can safely be administered to cancer patients and induce significant immunologic and clinical responses. Moreover, it has been demonstrated that DCs are related to clinical stage, invasion, metastasis and prognosis of gastric cancer. DC-based tumor vaccines become a new effective immunoadjuvant therapy for gastric cancer. Cellular & Molecular Immunology. 2004;1(5):351-356. | YugangWu LiangWang YanyunZhang | 2004 | Cellular & Molecular Immunology2004，1，5： | 13 |
| 3 | Stable transfection of extrinsic Smac gene enhances apoptosis-inducing effects of chemotherapeutic drugs on gastric cancer cells显示文摘AIM: TO explore the feasibility of enhancing apoptosis-inducing effects of chemotherapeutic drugs on human gastric cancer cells by stable transfection of extrinsic Smac gene. METHODS: After Smac gene was transferred into gastric cancer cell line MKN-45, subclone cells were obtained by persistent G418 selection. Cellular Smac gene expression was determined by RT-PCR and Western blotting. After treatment with mitomycin (MMC) as an apoptotic inducer, in vitro cell growth activities were investigated by trypan blue-staining method and MI-I colorimetry. Cell apoptosis and its rates were determined by electronic microscopy, annexin V-FITC and propidium iodide staining flow cytometry. Cellular caspase-3 protein expression and its activities were assayed by Western blotting and colorimetry. RESULTS: When compared with MKN-45 cells, the selected subclone cell line MKN-45/Smac had significantly higher Smac mRNA (3.12±0.21 vs0.82:1:0.14, t=7.52, P<0.01) and protein levels (4.02±0.24 vs0.98:1:0.11, t=8.32, P<0.01). After treatment with 10μg/mL MMC for 6-24 h, growth inhibition rate of MKN-45/Smac (15.8±1.2-54.8±2.9%) was significantly higher than that of MKN-45 (5.8±0.4-24.0±1.5%, t = 6.42, P<0.01). Partial MKN-45/Smac cancer cells presented characteristic morphological changes of apoptosis under the electronic microscope with an apoptosis rate of 36.4=1=2.1%, which was significantly higher than that of MKN-45 (15.2±0.8%, t = 9.25, P<0,01). Compared with MKN-45, caspase-3 expression levels in MKN-45/Smac were improved significantly (3.39±0.42 vs 0.96:1:0.14, t=8.63,P<0.01), while its activities were 3.25 times as many as those of MKN-45 (0.364±0.010 vs 0.112:1:0.007, t= 6.34, P<0.01). CONCLUSION: Stable transfection of extrinsic Smac gene and its over-expression in gasbic cancer cell line can significantly enhance cellular caspase-3 expression and activities, ameliorate apoptosis-inducing effects of mitomycin C on cancer cells, which is a novel strategy to improve chemotherapeutic effects on gastric cancer. | Li-DuanZheng Qiang-SongTong LiangWang JunLiu WeiQian | 2005 | World Journal of Gastroenterology2005，11，1： | 5 |
| 4 | Clinical significance of human kallikrein 10 gene expression in colorectal cancer and gastric cancer显示文摘 | BoFeng Wei‐BinXu Min‐HuaZheng Jun‐JunMa QuCai YiZhang JunJi Ai‐GuoLu YingQu Jian‐WenLi Ming‐LiangWang Wei‐GuoHu Bing‐YaLiu Zheng‐GangZhu | 2006 | Journal of Gastroenterology and Hepatology2006，，10： | 1 |
| 5 | Connexin 43 Reverses Malignant Phenotypes of Glioma Stem Cells by Modulating E‐Cadherin显示文摘 | Shi‐CangYu Hua‐LiangXiao Xue‐FengJiang Qing‐LiangWang YanLi Xiao‐JunYang Yi‐FangPing Jiang JieDuan Jian‐YongJiang Xian‐ZongYe Sen‐LinXu Yang‐HongXin Xiao‐HongYao Jian‐HongChen Wei‐HuaChu WeiSun BingWang Ji MingWang XiaZhang Xiu‐WuBian | 2012 | STEM CELLS2012，，2： | 1 |
| 6 | Sedimentary Enzyme Kinetics of Land/Water Ecotones with Reed Domination显示文摘 | LiangWang WeidongWang | 2010 | Clean Soil Air Water2010，，2： | 1 |
| 7 | Recent progress and understanding of the molecular mechanisms of the rice–Magnaporthe oryzae interaction显示文摘 | JINLINGLIU XUEJUNWANG THOMASMITCHELL YAJUNHU XIONGLUNLIU LIANGYINGDAI GUO‐LIANGWANG | 2010 | Molecular Plant Pathology2010，，3： | 1 |
| 8 | Securitization of Longevity P, fsk in leverse Mortgages 显示文摘 | LiangWang Valdez Emilianoand Piggott John | 2007 | 2007，，： | 1 |
| 9 | HYAL1 overexpression is correlated with the malignant behavior of human breast cancer显示文摘 | Jin‐XiangTan Xiao‐YiWang Hong‐YuanLi Xin‐LiangSu LiangWang LiangRan KeZheng Guo‐ShengRen | 2011 | Cancer2011，，6： | 1 |
| 10 | Elevated Admission Microalbuminuria Predicts Poor Myocardial Blood Flow and 6‐Month Mortality in ST‐Segment Elevation Myocardial Infarction Patients Undergoing Primary Percutaneous Coronary Intervention显示文摘 | Jia WeiChen Yong LiangWang Hong WeiLi | 2012 | Clin Cardiol2012，，4： | 1 |
| 11 | Cardioprotective and chemosensitizing effects of novel Danshensu derivatives显示文摘OBJECTIVE To investigate the cardioprotective effect of novel danshensu derivatives against doxorubicin(Dox)cardiotoxicity and their synergistic anti-tumor effect with Dox on breast cancer cells.METHODS Two new Danshensu derivatives were synthesized by conjugation with tetramethylpyrizine and/or4-(3-thioxo-3 H-1,2-dithiol-4-yl)-benzoic acid and tested for protective effects against Dox induced cardiotoxicity in cell and zebrafish.H9c2 cardiomyoblasts were co-treated with Dox and Danshensu derivatives for 24 h and then were measured for cell viability and cytotoxicity by MTT and LDH assays.The expression levels of mitochondrial biogenesis related proteins PGC-1α,NRF-1and Nrf2 were detected by Western blotting and qPCR.Moreover,in a Dox-induced cardiotoxicity model of zebrafish,zebrafish embryos were treated with Dox for 36 h,followed by measurement of numerous ventricular function parameters including heart rate,stroke volume,cardiac output and fractional shortening.In addition,the synergistic anti-tumor effects of the Danshensu derivatives and Dox had been studied in MCF-7 breast cancer cells.The effects of the Danshensu derivatives on the cell death and metabolism of MCF-7 cells were measured using apoptosis assay and Seahorse Metabolic Analyzers respectively.RESULTS Our results showed that the Danshensu derivatives were more potent than the parental compounds in ameliorating Dox-induced cytotoxicity in H9c2 cells and significantly preserving stroke volume of heart function in Dox-treated zebrafish.Further mechanistic studies identified that the danshensu derivatives increased mitochondrial copy numbers and protein expressions of PGC-1α,NRF-1 and Nrf2 in H9c2 cells.In addition,the Danshensu derivatives enhanced Dox-induced apoptosis,and decreased glycolysis and mitochondrial function in MCF-7 tumor cells.CONCLUSION Our results revealed that two new Danshensu derivatives displayed promising cardioprotective effects against Dox induced cardiotoxicity both in vivo and in vitro,at least partially through activating mitochondrial biogenesis.Also,the new Danshensu derivatives potentiated the anticancer effects of Dox in breast tumor cells involving induction of glycolytic inhibition and mitochondrial dysfunction. | LiangWANG Lu-chenSHAN Guo-zhenCUI JudyYuet-WaCHAN Jing-jingLI Qing-wenZHANG MaggiePui-ManHOI Yu-qiangWANG SimonMing-YuenLEE | 2015 | 中国药理学与毒理学杂志2015，29，S1： | 0 |
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