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| 1 | Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15显示文摘AIM: Ursolic acid (UA) and oleanolic acid (OA) aretriperpene acids having a similar chemical structure and aredistributed wildly in plants all over the world. In recentyearn, it was found that they had marked anti-tumor effects.There is little literature currently available regarding theireffects on colon carcinoma cells. The present study wasdesigned to investigate their inhibitory effects on humancolon carcinoma cell line HCT15 METHODS: HCT15 cells were cultured with different drugs.The treated cells were stained with hematoxylin-eosin andtheir morphologic changes observed under a lightmicroscope. The cytotoxicity of these drugs was evaluatedby tetrazolium dye assay. Cell cycle analysis was performedby flow cytometry (FCM). Data were expressed as means +SEM and Analysis of variance and Student' t-test forindividual comparisons.RESULTS: Twenty-four to 72 h after UA or OA 60 μmol/Ltreatment, the numbers of dead cells and cell fragmentswere increased and most cells were dead at the 72 nd hour.The cytotoxicity of UA was stronger than that of OA.Seventy-eight hours after 30 μmol/L of UA or OA treatment,a number of cells were degenerated, but cell fragments wererarely seen. The IC50 values for UA and OA were 30 and 60μmol/L, respectively. Proliferation assay showed thatproliferation of UA and OA-treated cells was slightlyincreased at 24 h and significantly decreased at 48 h and 60h, whereas untreated control cells maintained anexponential growth curve. Cell cycle analysis by FCMshowed HCT15 cells treated with UA 30 and OA 60 for 36 h and72 h gradually accumulated in G0/G1 phase (both drugs P< 0.05 for 72 h), with a concomitant decrease of cell populationsin S phase (both drugs P< 0.01 for 72 h) and no detectableapoptotic fraction.CONCLUSION: UA and OA have significant anti-ttumor activity.The effect of UA is stronger than that of OA. The possiblemechanism of action is that both drugs have an inhibitoryeffect on tumor cell proliferation through cell-cycle arrest. | LiJ GuoWJ 等 | 2002 | World Journal of Gastroenterology2002，8，3： | 80 |
| 2 | 芬兰研究型教师教育述评显示文摘芬兰教育成功的重要原因之一是芬兰的研究型教师教育。新的教师教育课程要求所有的师范生都要开展一项他们自己的研究,以发展理论与实践结合的专业能力。本文介绍了芬兰研究型教师教育发展的背景、基本特点,以及实施的主要环节。 | Jouni Vlijrvi 陆璟 | 2009 | 上海教育科研2009，，1： | 20 |
| 3 | Practiceandanalysisofdrugcounselingandpatienteducationaboutwarfarin[J]显示文摘 | LIJ LVQz | | 中国药学杂志0，，： | 2 |
| 4 | Association of two polymorphisms of tumor necrosis factor gene with acute severe pancreatitis显示文摘 | Zhang D LiJ Jiang ZW | 2003 | J Surg Res2003，112，2： | 1 |
| 5 | A folate receptor-targeting nanoparti- cle minimizes drug resistance in a human cancer model显示文摘 | Wang X LiJ Wang Y | 2011 | ACS Na- no2011，5，8： | 1 |
| 6 | Modeling mixed flow in storm sewers显示文摘 | LiJ Mccoruodale A | | 0，，11： | 1 |
| 7 | Structural characteristics and hydration kinetics of modified steel slag 显示文摘 | LiJ X Yu Q J WeiJ X | 2011 | Cement and Concrete Research2011，41，3： | 1 |
| 8 | Comparativestudyofdifferentproce-duresfortheseparationofperipheralbloodmononuclearcellsincytokine-inducedkillercellimmunotherapyforhepatocarcinoma显示文摘 | LiuH LiJ WangF etal | 2015 | TumourBiol2015，36，4： | 1 |
| 9 | Enrich and seal radionu- elides in magnetic agarose mierospheres 显示文摘 | LiJ Guo Z Zhang S | 2011 | Chemical Engineering Journal2011，172，2： | 1 |
| 10 | Effect of a carbohydrate-containing late-eveningsnack on energy metabolism and fasting substrate utilization in adultswith acute-on-chronic liver failure due to Hepatitis B显示文摘 | Hou LiJ LuJ | 2013 | EurJ ClinNutr2013，67，12： | 1 |
| 11 | Catalytic dechlorination of 2, 4-dichlorophenol by Ni/ Fe nanoparticles in the presence of humic acid: Inter- mediate products and some experimental parameters 显示文摘 | Zhang Zhen LiJ Xiaoshu Baig Shams A/i | 2014 | JExpNanosci2014，9，6： | 1 |
| 12 | AqueoushumoringlaucomatouseyescontainsanincreasedlevelofTGF-betaz显示文摘 | TripathiRC LiJ ChanWF etal | 1994 | ExpEyeRes1994，59，6： | 1 |
| 13 | AQP5 is a new transcriptional target of Dotla and a regulator of AQP2显示文摘 | Wu H Chen L Zhang X Zhou Q LiJ M Berger S | 2013 | PLoS One2013，8，53： | 1 |
| 14 | Experiment and simulations of directionally annealed ODS MA 754显示文摘 | BAKER I IIIESCU B LIJ FROST H J | 2008 | Mater Sci Eng A2008，492，12： | 1 |
| 15 | Prevalenceofprehypertension,hypertensionand,associatedriskfactorsinMongolianandHanChinesepopulationsinNortheastChina显示文摘 | SunZ ZhengL XuC LiJ ZhangX LiuS etal | 2008 | IntJCardiol2008，128，25： | 1 |
| 16 | Echinococcosis显示文摘 | McManus DP Zhang W LiJ | 2003 | Lancet2003，362，： | 1 |
| 17 | ExpressionofgrowthfactormRNAbyhumanTenon'capsulefibroblasts显示文摘 | TripathiRC LiJ ChalamKV etal | 1996 | ExpEyeRes1996，63，： | 1 |
| 18 | Gene expression profile of ratadipose tissue at theonset of high-fat-diet obesity显示文摘 | LIJ YU X PAN W | 2002 | Am J Physiol Endocrinol Metab2002，282，： | 1 |
| 19 | 显示文摘 | Wang WX LiJ | 2011 | Advanced Materials Research2011，，： | 1 |
| 20 | Improved UV Stability of Antibacterial Coatings with N-halamine/TiOz 显示文摘 | LiJ LiR Du J M | 2013 | Cellulose2013，20，4： | 1 |
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