|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 内毒素诱导大鼠葡萄膜炎的组织平片和切片研究显示文摘目的:探讨内毒素诱导的视网膜脉络膜改变和平片、切片结果的差异。方法:从内毒素注射的Lewis鼠制备视网膜和脉络膜-巩膜复合体平片以及眼组织冰冻切片,并进行免疫组织化学染色。结果:平片上显示,注射后4小时单核细胞浸润,随后,大量的单核巨噬细胞浸润至整个视网膜和脉络膜,脉络膜中尚有MHC-Ⅱ类抗原阳性细胞增多;切片上发现,于注射后一定时期,视网膜和脉络膜中有上述细胞浸润。结论:平片技术为视网膜脉络膜原发性严重受累提供了确凿的证据,内毒素诱导的葡萄膜炎可作为人类全葡萄膜炎的动物模型。 | 杨培增 Alex F. de Vos Lidy Broersma Aize kijlstra | 1996 | 中华眼底病杂志1996，12，1： | 16 |
| 2 | Bone tissue engineering via growth factor delivery:from scaffolds to complex matrices显示文摘In recent years,bone tissue engineering has emerged as a promising solution to the limitations of current gold standard treatment options for bone related-disorders such as bone grafts.Bone tissue engineering provides a scaffold design that mimics the extracellular matrix,providing an architecture that guides the natural bone regeneration process.During this period,a new generation of bone tissue engineering scaffolds has been designed and characterized that explores the incorporation of signaling molecules in order to enhance cell recruitment and ingress into the scaffold,as well as osteogenic differentiation and angiogenesis,each of which is crucial to successful bone regeneration.Here,we outline and critically analyze key characteristics of successful bone tissue engineering scaffolds.We also explore candidate materials used to fabricate these scaffolds.Different growth factors involved in the highly coordinated process of bone repair are discussed,and the key requirements of a growth factor delivery system are described.Finally,we concentrate on an analysis of scaffold-based growth factor delivery strategies found in the recent literature.In particular,the incorporation of two-phase systems consisting of growth factor-loaded nanoparticles embedded into scaffolds shows great promise,both by providing sustained release over a therapeutically relevant timeframe and the potential to sequentially deliver multiple growth factors. | Tinke-Marie De Witte Lidy E.Fratila-Apachitei Amir A.Zadpoor Nicholas A.Peppas | 2018 | Regenerative Biomaterials2018，5，4： | 12 |
| 3 | 实验性自身免疫性葡萄膜视网膜炎中浸润细胞的表型及其凋亡的研究显示文摘目的 探讨实验性自身免疫性葡萄膜视网膜炎 (experimental autoimmune uveoretinitis,EAU)中参与的细胞表型及其凋亡。 方法 用光感受器间维生素 A类结合蛋白 (interphotoreceptorretinoid- binding protein,IRBP)免疫 16只 L ewis鼠后 ,于眼组织切片和平片上进行免疫组织化学染色和原位凋亡染色 ,所用抗体为抗单核细胞、巨噬细胞 (EDI)、MHC- 类抗原 (OX6 )、T淋巴细胞 (R73)的单克隆抗体 ,所用原位凋亡试剂盒为 TACS1Klenow。 结果 用 IRBP免疫 L ewis鼠后 ,16只鼠中 12只发生了临床可见的葡萄膜炎 ,炎症平均得分为 1.2 9± 0 .7级 ;免疫组织化学染色发现葡萄膜和视网膜中有大量的单核细胞、淋巴细胞及 MHC- +细胞浸润 ,这些组织中均可见浸润细胞的凋亡 ,虹膜睫状体中凋亡细胞明显多于脉络膜和视网膜中的凋亡细胞。 结论 单核巨噬细胞、淋巴细胞和 MHC- +细胞均参与了 EAU的形成 ,在 EAU早期即有浸润细胞发生凋亡 ,此可能是导致这种炎症迅速消退的重要机制。 | 杨培增 Nicole H Herzberg 周红颜 黄祥坤 谢楚芳 金浩丽 籍利 Lidy Broersma Mavc D de Smet Aize Kijlstra | 2000 | 中华眼底病杂志2000，16，1： | 9 |
| 4 | Lipopolysaccharide induced intestinal epithelial injury:a novel organoids-based model for sepsis in vitro显示文摘Background: Advances in organoid culture technology have provided a greater understanding of disease pathogenesis, which has been rarely studied in sepsis before. We aim to establish a suitable organoids-based intestinal injury model for sepsis.Methods: Stable passaged organoids were constructed and pre-treated with lipopolysaccharide (LPS) to mimic sepsis-induced intestinal injury. The LPS-induced sepsis model was used as a reference. We used quantitative real-time polymerase chain reaction to evaluate the RNA levels of inflammatory factors and antimicrobial peptides. Enzyme-linked immunosorbent assay was used to evaluate the protein levels, hematoxylin and eosin staining was used to evaluate the pathology of the small intestine of mice, and immunohistochemistry and immunofluorescence were used to evaluate the intestinal epithelial barrier function. Perkin Elmer OperettaTM was used to obtain high-resolution images of three-dimensional organoids.Results: An LPS concentration >150 μg/mL after 24 h was identified to cause organoid growth restriction. The fluorescence intensity of zonula occludens-1 and occludins at LPS concentrations >100 μg/mL decreased significantly after 24 h. After LPS stimulation for 8 h, the RNA expression levels of interleukin (IL)-1α, tumor necrosis factor alpha, granulocyte-macrophage colony-stimulating factor, IL-6, and regenerating islet-derived protein 3 alpha, beta, and gamma increased. These results resembled those of intestinal epithelial layer alterations in a mouse sepsis model. For IL-10, the RNA expression level increased only when the LPS level >200 μg/mL for 24 h.Conclusions: This study provides the primary intestinalin vitro model to study the effects of LPS-induced intestinal injury resembling sepsis. This model provides a platform for immune associated mechanism exploration and effective drug screening. | Sisi Huang Sheng Zhang Limin Chen Xiaojun Pan Zhenliang Wen Yizhu Chen Lidi Zhang Jiao Liu Dechang Chen | 2022 | Chinese Medical Journal2022，135，18： | 3 |
| 5 | IL-27 inhibits HIV-1infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation显示文摘 | Dai L Lidie KB Chen Q | 2013 | Journal of Experimental Medicine2013，11，： | 1 |
| 6 | Experimental study of upward flame spread over canvas hung in the air显示文摘 | Zhu Guo-qing Zhang Guo-wei Huang Lidi | 2014 | Journal of Ap- plied Fire Science2014，23，3： | 1 |
| 7 | Hyperplasia of the lymphoepithelium of NALT in rats but not in mice upon 28-day exposure to 15<ce:hsp sp='0.25'/>ppm formaldehyde vapor显示文摘 | C. Frieke Kuper Lidy van Oostrum Lan Ma-Hock Stefan Durrer Ruud A. Woutersen | 2009 | Experimental and Toxicologic Pathology2009，，1： | 1 |
| 8 | Periodic Motions and Grazing in a Harmonically Forced, Piecewise, Linear Oscillator with Impacts显示文摘 | Albert C J L Chen Lidi | 2005 | Chaos Soiltons & Fractals2005，24，2： | 1 |
| 9 | Original identification and application of the geranium显示文摘 | GAOLH GAOFL LIDY | 1994 | 中国林副特产1994，29，2： | 1 |
| 10 | Periodic motions and graz-ing in a harmonically forced,piecewise,linear oscillatorwith impacts显示文摘 | Albert C J Luo Chen Lidi | 2005 | Chaos Solitons&Fractals2005，24，2： | 1 |
| 11 | On set of failure in aluminum honeycombs under general in-plane loading显示文摘 | Triantafyl lidis N Sch raad M W | 1998 | Journal of the Mechanics and Physics of Solids1998，，46： | 1 |
| 12 | Defectiveangiogenesis inmice lacking endoglin显示文摘 | LiDY Sorensen L K Breoke BS | 1999 | Science1999，284，5419： | 1 |
| 13 | A randomised controlled trial into the effects of food on ADHD显示文摘 | Lidy MJ Huub FJ Jan K | 2009 | Eur Child Adolesc Psychiatry2009，18，： | 1 |
| 14 | Radiant Flash Pyrolysis of Cellulose--Evidence for the Formation of Short Life Time Intermediate Liquid Species 显示文摘 | Boutin O Ferrer M LIDI J | 1998 | Journal of Analytical and Applied Pyrolysis1998，47，1： | 1 |
| 15 | A novel concept for convective heat transfer enhancement 显示文摘 | GUOZY LIDY WANG B X | 1998 | International Journal of Heat and Mass Transfer1998，41，14： | 1 |
| 16 | Periodic Mmotions and Ggrazing in a Harmonically Forced, Piecewise, Llinear Oscillator with Impacts显示文摘 | Albert C J L Chen Lidi | 2005 | Chaos Soil- tons - Fractals2005，24，2： | 1 |
| 17 | Photovoltaic-Battery-Powered DC Bus Sys- tem for Common Portable Electronic Devices 显示文摘 | DYLAN DAHCHUAN LU VASSILIOS G AGE- LIDIS | 2009 | IEEE Transactions on Power Electronics2009，24，3： | 1 |
| 18 | Personality as a predictor of First-Semester Adjustment to college: the meditational role of perceived social support 显示文摘 | Lidy KM Kahn J H | 2006 | Journal of College Counseling2006，9，2： | 1 |
| 19 | FallbackFoodsofTemperate-Liv-ingPrimates:ACaseStudyonSnub-NosedMonkeys显示文摘 | GrueterC LiDY RenBP etal | 2009 | AmericanJournalofPhysicalAnthropology2009，140，： | 1 |
| 20 | The studies of GLUT1 expression and neuron apoptosis in neonatal hypoxic-ischemia brain damage rat model显示文摘 | Zhang X L LiDY Zhao F Y | | 0，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员