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| 1 | Oral Immunization of Mice With Vaccine of Attenuated Salmonella typhimurium Expressing Helicobacter pylori Urease B Subunit显示文摘Objective To prepare the live recombinant vaccine of attenuated Salmonella typhimurium SL3261 expressing Helicobacterpylori (H. pylori) B subunit (UreB) and to determine whether it could be used as an oral vaccine against H. pylori infection. Methods Using genomic DNA of H. pylori Sydney strain (SS1) as template, the H. pylori UreB gene fragment was amplified by PCR and subcloned into the expression vector pTC01. The recombinant plasmid pTC01-UreB was then transferred into LB5000 to obtain modified forms, and further conversed into the attenuated Salmonella typhimurium SL3261 to obtain recombinant SL3261/pCT01-UreB as an oral immunization reagent, which was then used to orally immunize Balb/c mice twice at a three-week interval. Twelve weeks later, anti-UreB IgA antibodies in intestinal fluid and IgG antibodies in sera were determined by ELISA. The relating data in control groups (including body weight, gastric inflammation, etc.) were also collected. Results The sequencing analysis showed that the UreB gene fragment amplified by PCR was consistent with the sequence of the H. pylori UreB gene. The restriction enzyme digestion revealed that the correct pTC01-UreB was obtained.SDS-PAGE and Western blot showed that a 61KD protein was expressed in SL3261/pTC01-UreB, which could be recognized by anti-H. pylori UreB antiserum and was absent in the control containing only Salmonella typhimurium SL3261 strain. The multiple oral immunization with SL3261/pTC01-UreB could significantly induce H. pylori specific mucosal IgA response as well as serum IgG responses. IFN-γ and IL-10 levels were significantly increased in SL3261/pTC01-UreB group, and no obvious side effect and change in gastric inflammation were observed. Conclusion The attenuated vaccine of Salmonella typhimurium expressing H. pylori UreB can be used as an oral vaccine against H. pylori infection. | XING-LONG YANG WEN-CHAO LID Wu-WEI YANG DONG ZHONG Yu-Hu LIU JING-DONG ZHANG JIAN-HUI JIANG SHAN-SHAN LI | 2005 | Biomedical and Environmental Sciences2005，18，6： | 6 |
| 2 | Short-term Safety and Efficiency of Cryoablation for Rena Sympathetic Denervation in a Swine Model显示文摘Background: Renal sympathetic nerves are involved in the reflective activation of the sympathetic nervous system in circulatory control. Catheter-based renal denervation (RDN) ameliorated treatment-resistant hypertension safely, but 10%-20% of treated patients are nonresponders to radiofrequency denervation. The purpose of this study was to investigate the safety and efficiency of cryoablation for sympathetic denervation in a swine model and to explore a new way of RDN. Methods: Seven swines randomly assigned to two groups: Renal cryoablation (CR) group and control group. The control group underwent renal angiogram only. The CR group underwent renal angiogram plus bilateral renal cryoablation. Renal angiograms via femoral were performed before denervation, after denervation and prior to the sacrifice to access the diameter of renal arterial and the pressure of aorta abdominalis. Euthanasia of the swine was performed on 28-day to access norepinephrine (NE) changes of the renal cortex and the changes of renal nerves. Results: Cryoablation did not induce severe complications at any time point. There was no significant change in diameter of renal artery. CR reduced systolic blood pressure (BP) from 145.50 ± 9.95 mmHg at baseline to 119.00 ± 14.09 mmHg. There was a slight but insignificant decrease in diastolic BP. The main nerve changes at 28-day consisted of necrosis with perineurial fibrosis at the site of CR exposure in conjunction with the nerve vacuolation. Compared with the control group, renal tissue NE of CR group decreased by 89.85%. Conclusions: Percutaneous catheter-based cryoablation of the renal artery is safe. CR could effectively reduce NE storing in the renal cortex, and the efficiency could be maintained 28-day at least. | Meng Ji Li Shen Yi-ZheWu Zhi-Feng Yao Jia-Sheng Yin Jia-Hui Chen Jian-Guo Jia Ling-Juan Qiao Peng LiD Jun-Bo Ge | 2015 | Chinese Medical Journal2015，，6： | 5 |
| 3 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 4 | Self-assembling peptide nanofiber hydrogels for central nervous system regeneration显示文摘 | Xi LID Bin PI Hui WANG Xiu-Mei WANG | 2015 | Frontiers of Materials Science2015，9，1： | 2 |
| 5 | Theoretical studies on molecular structure and vibrational spectra of copper phthalocyanine 显示文摘 | LID C PENG Z H | 2005 | Vibrational Spectroscopy2005，39，2： | 1 |
| 6 | Mechanism of induction of cytochrome p450ac(p450j) in chemically induced and spontaneously diabetic rats显示文摘 | Dong Z Hong J Ma Q LiD | 1988 | Arch Biochem Biophys1988，263，： | 1 |
| 7 | Isotope variability of particulate organic matter at the PN section in the East China Sea显示文摘 | WU Y ZHANG J LID J et | 2003 | Biogeochemistry2003，65，1： | 1 |
| 8 | Preparation and characterization of a pan nanofibre containing ag nanoparticles via electrospinning 显示文摘 | YANG Q B LID M HONG Y L | 2003 | Synthetic Metals2003，，137： | 1 |
| 9 | Maximum number of edges in spanning eulerian subgraph显示文摘 | LID X LI D Y MAO J Z | 2004 | Discrete Mathematics2004，274，1： | 1 |
| 10 | Osteonecrosis of the fem- oral head:a prospective study with MRI 显示文摘 | Sakamoto M Shimizu K Lid S | 1997 | J Bone Joint Surg Br1997，79，3： | 1 |
| 11 | Experimental investigation of air entrainment in free-falling particle plumes显示文摘 | LID Z COOPER P WYPYCH P W | 2007 | Part Sci Technol2007，25，4： | 1 |
| 12 | Controlled growth of copper germanate nanoflowers显示文摘 | LID K PEIL Z YANG Y | 2011 | Journal of Advanced Microscopy Research2011，6，2： | 1 |
| 13 | Inhibitory effect of Lycium barbarum polysaccharides on cell apoptosis and senescence is potentially mediated by the p53 signaling pathway 显示文摘 | XIA G XIN N LID W | 2014 | Mol Med Rep2014，9，4： | 1 |
| 14 | Two new cucurbitane- type glycosides obtained from roots of Siraitia grosvenori SWINGLE 显示文摘 | LID P EL-AASR M IKEDA T et 01 | 2009 | Chemical and Pharmaceutical Bulletin2009，57，8： | 1 |
| 15 | On similarity preserving feature selection显示文摘 | ZHAO Z WANG L LID H | 2013 | IEEE Transactions on Knowledge and Data Engineering2013，25，3： | 1 |
| 16 | Research on electromagnetic interfer- ence resistance of piezoelectric vibration acceleration sensor显示文摘 | HAO C LID H | 2014 | Ferroelectrics2014，，460： | 1 |
| 17 | A functional bone morphogenetic protein system in the ovary 显示文摘 | SHIMASAKI S ZACHOW R J LID M | 1999 | PNAS1999，96，13： | 1 |
| 18 | Maternal autoantibody levels in congenital heart block and potential prophylaxis with antiinflammatory agents 显示文摘 | Tunks liD Clowse ME Miller SG | 2013 | Am J Obstet Gynecol2013，208，1： | 1 |
| 19 | Photoinhibition and active ox ygen species production in detached apple leaves during dehy dration显示文摘 | JIA H S HAN Y Q LID Q | 2003 | Photosynthetica2003，41，1： | 1 |
| 20 | The influence of different doping elements on microstructure, piezoelectric coefficient and resistivity of sputtered ZnO film 显示文摘 | WANG X B SONG C LID M | 2006 | Applied Surface Science2006，253，： | 1 |
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