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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | NEW ALDALOIDS TONGOLINE AND TONGOLININE FROMDELPHINIUM TONGOLENSE显示文摘NEWALDALOIDSTONGOLINEANDTONGOLININEFROMDELPHINIUMTONGOLENSELanHE;YaoZuCHEN(TheChemistryDepartmentofLanzhouUniversity,Lanzhou7... | He, L Chen, YZ Ding, LS Li, BG | 1996 | Chinese Chemical Letters1996，7，6： | 4 |
| 3 | Risk factors and natural history of breast cancer in younger Chinese women显示文摘AIM: To investigate the age differences in the risk factors, clinicopathological characteristics and patterns of treatment of female breast cancer patients. METHODS: Seven thousand one hundred and fiftytwo women with primary breast cancer from the Hong Kong Breast Cancer Registry were recruited after receiving patients' consent, they were asked to complete standardized questionnaires which captured their sociodemographic characteristics and risk factors associated with breast cancer development. Among them, clinicopathological data and patterns of treatment were further collected from medical records of 5523 patients with invasive breast cancers. Patients were divided into two groups according to the age at diagnosis: younger(< 40 years old) vs older patients(≥ 40 years old) for subsequent analyses.RESULTS: Analysis on the sociodemographic characteristics and exposure to risk factors were performed on 7152 women with primary breast cancer and the results revealed that younger patients were more likely to have unhealthy lifestyles; these include a lack of exercise(85.4% vs 73.2%, P < 0.001), having high stress in life(46.1% vs 35.5%, P < 0.001), having dairy/meat-rich diets(20.2% vs 12.9%, P < 0.001),having alcohol drinking habit(7.7% vs 5.2%, P = 0.002). Younger patients were also more likely to have hormone-related risk factors including nulliparity(43.3% vs 17.8%, P < 0.001) and an early age at menarche(20.7% vs 13.2%, P < 0.001). Analyses on clinicopathological characteristics and patterns of treatment were performed on 5523 women diagnosed with invasive breast cancer. The invasive tumours in younger patients showed more aggressive pathological features such as having a higher percentage of grade 3 histology(45.7% vs 36.5%, P < 0.001), having a higher proportion of tumours with lymphovascular invasion(39.6% vs 33.2%, P = 0.003), and having multifocal disease(15.7% vs 10.3%, P < 0.001); they received different patterns of treatment than their older counterparts.CONCLUSION: Younger patients in Hong Kong are more likely to encounter risk factors associated with breast cancer development and have more aggressive tumours than their older counterparts. | Winnie Yeo Hang-Mei Lee Amy Chan Emily YY Chan Miranda CM Chan Keeng-Wai Chan Sharon WW Chan Foon-Yiu Cheung Polly SY Cheung Peter HK Choi Josette SY Chor William WL Foo Wing-Hong Kwan Stephen CK Law Lawrence PK Li Janice WH Tsang Yuk Tung Lorna LS Wong Ting-Ting Wong Chun-Chung Yau Tsz-Kok Yau Benny CY Zee | 2014 | World Journal of Clinical Oncology2014，5，5： | 4 |
| 4 | Epidemiologic data of renal diseases from a single unit in China:analysis based on 13,519 renal biopsies显示文摘 | Li LS Liu ZM | 2004 | Kidney Int2004，66，： | 1 |
| 5 | Ep idemiologic data of renal diseases from a single unit in China:analysis based on 13,519 renal biop sies显示文摘 | Li LS LIU ZH | 2004 | Kidney Int2004，66，3： | 1 |
| 6 | Resveratoral augments the canonical Wnt signaling pathway in promoting osteoblastic differentiation of multiptotent mesenchymal cells显示文摘 | Zhou HB Shang LS Li X | 2009 | Exp Cell Res2009，315，： | 1 |
| 7 | Epidemiologic data of renal diseasesfrom a single unit in China: analysis based on 13,519renal biop- sies 显示文摘 | Li LS Liu ZH | 2004 | Kidney Int2004，66，3： | 1 |
| 8 | Gene expression in human neural stem cells: effects of leukemia inhibitory factor显示文摘 | Wright LS Li J Caldwell MA | 2003 | J Neurochem2003，86，1： | 1 |
| 9 | Epidemiologic data of renal diseases from a single unit in China:analysis based on 13,519 renal biopsies显示文摘 | Li LS Liu ZH | 2004 | Kidney Int2004，66，3： | 1 |
| 10 | End-stage renal disease in China 显示文摘 | Li LS | 1996 | Kidney Int1996，4，1： | 1 |
| 11 | Epidemiologic data of renal diseases from a single unit in China:analysis based on 13 519 renal biopsies显示文摘 | Li LS Liu ZH | | 0，，03： | 1 |
| 12 | Epidemiologic data of renal diseases from a single unit in china: analysis based on 13 519 renal biopsies显示文摘 | Li LS Liu ZH | 2004 | Kidney lnt2004，66，3： | 1 |
| 13 | Anatomical studies of the urethral plate:why preservation of the urethral plate is important in hypospadias repair显示文摘 | Erol A Baskin LS Li YW | 2000 | BJU Int2000，85，6： | 1 |
| 14 | Prostate cancer radiosensiti- zation through poly( adp - ribose ) polymerase - 1 hyperacti- vation 显示文摘 | DQNG Y BEY EA LI LS | 2010 | Cancer Res2010，70，20： | 1 |
| 15 | Rhein reverses the diabetic phenotype of mesangial cells over-expressing the glucose transporter(GLUT1)by inhibiting the hexosamine pathway显示文摘 | Zheng JM Zhu JM Li LS | 2008 | Br J Pharmacol2008，153，7： | 1 |
| 16 | Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony(Paeonia section Moutan)cultivars by GC-MS显示文摘 | Li SS Yuan RY Chen LG Wang LS Hao XH Wang LJ Zheng XC Du H | 2015 | Food Chem2015，173，15： | 1 |
| 17 | A Safe and Brief Way for Preparing Anhydrous LnCl_3 (Ln=Sc, Y, La to Lu)显示文摘@李瑛@王林山@王育红 | Li, Y Wang, LS Wang, YH | 2000 | Rare Metals2000，19，2： | 1 |
| 18 | Epidemiologie data of renal diseases from a single unit in China: analysis based on 13,519 renal biopsies 显示文摘 | Li LS Liu ZH | 2004 | Kidney Int2004，66，3： | 1 |
| 19 | Epidemiologic data of renal diseases from a single unit in china : analysis based on 13519 renal biopsies显示文摘 | Li LS Liu ZH | 2004 | Kidney Int2004，66，3： | 1 |
| 20 | Epidemiologic deta of renal diseases froma single unite in China analysis based on 13519 renalbiopsies显示文摘 | Li LS Liu ZH | 2004 | Kidney Int2004，66，3： | 1 |
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