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| 1 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 2 | Heteropolyanion basedionic liquids:reaction-induced self-separation catalystsfor esterification显示文摘 | Leng Y Wang J Zhu D R | 2009 | Angew Chem Int Ed2009，48，1： | 1 |
| 3 | Miti- gating methane production from ruminants; effect of calcium nitrate as modifier of the fermentation in an in vitro incubation using cassava root as the energy source and leaves of cassava or Mimosa pigra as source of protein 显示文摘 | INTHAPANYA S PRESTON T R LENG R A | 2011 | Livestock Research for Rural Development2011，23，2： | 1 |
| 4 | Mi R-718 represses VEGF and inhibits ovarian cancer cell progression显示文摘 | Leng R Zha L Tang L | 2014 | FEBS Lett2014，588，12： | 1 |
| 5 | Immunological study of hal domain of he- magglutinin of influenza hSnl virus 显示文摘 | Chiu F F Venkatesan N Wu C R Chou A H Chen H W Lian S P Liu SJ Huang C C Lian W C Chong P Leng C H | 2009 | Biochemical Biophys ResCommun2009，383，1： | 1 |
| 6 | Heteropolyanion-based i- onic hybrid solid:A green bulk-type catalyst for hydroxyl- ation of benzene with hydrogen peroxide 显示文摘 | Leng Y Wang J Zhu D R | 2011 | Chem Eng J2011，173，2： | 1 |
| 7 | TLR3signaling in macrophages is indispensable for the protective immunity of invariant natural killer T cells against enterovirus 71infection显示文摘 | Zhu K Yang J Luo K Yang C Zhang N Xu R Chen J Jin M Xu B Guo N Wang J Chen Z Cui Y Zhao H Wang Y Deng C Bai L Ge B Qin C F Shen H Yang C F Leng Q | 2015 | PLoS Pathog2015，11，10： | 1 |
| 8 | Endonasal endoscopic resection of an os odontoideum to decompress the cervicomedullary junction: a minimal access surgical technique 显示文摘 | Leng LZ Anand VK Hartl R | 2009 | Spine2009，34，4： | 1 |
| 9 | Insulin-like growth factor (IGF)-binding protein-6 inhibits IGF-II-induced but not basal proliferation and adhesion of L1M 1215 colon cancer cells 显示文摘 | Leng S L Leeding K S Whitehead R H | 2001 | Molecular and Cellular Endocrinology2001，174，12： | 1 |
| 10 | Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats显示文摘 | Leng X Zhan R Wang Y | | 0，，： | 1 |
| 11 | Photoexcited solitons and polarons in pemigraniline-base polymers 显示文摘 | Leng J M McCall R P CromackK R | 1993 | Phys Rev1993，48，15： | 1 |
| 12 | Structure-related optical properties of (Pb, La) (Zr, Ti) O3 thin films on indium tin oxide/quartz substrates显示文摘 | LENG W J YANG C R JI H | 2006 | J Appl Phys2006，100，8： | 1 |
| 13 | Relationships of lipoealin 2 with breast tumorigenesis and metastasis 显示文摘 | Leng X Wu Y Arlinghaus R B | 2011 | J Cell Physiol2011，226，2： | 1 |
| 14 | Quantative ruminant nutrition-a green house science 显示文摘 | Leng R A | 1993 | Aust J Agri Resour Eeon1993，44，： | 1 |
| 15 | Nitrogen metabolism in therumen显示文摘 | LENG R A NOLAN J V | 1984 | Journal of Dairy Science1984，67，5： | 1 |
| 16 | Electrical and optical properties of lanthanum-modified lead zirconate titanate thin films by radio-frequency magnetron sputtering显示文摘 | LENG W J YANG C R JI H | 2006 | J Appl Phys2006，100，10： | 1 |
| 17 | Duckweed-a potential high-protein feed resource for domestic animals and fish 显示文摘 | Leng RA Stambolie JH Bell R | 1995 | Livestock Res Rural Dev1995，7，1： | 1 |
| 18 | Nitrogen metabolism in the rumen 显示文摘 | Leng R A Nolan J V | 1984 | J Dairy Science1984，67，10： | 1 |
| 19 | Synthesis of Y-doped BaTiO3-(Bi1/2K1/2)TiO3 Lead-free Positive Temperature Coefficient of Resistivity Ceramics and Their PTC Effects显示文摘 | Leng S L Li G R Zheng L Y | 2009 | Journal of the American Ceramic Society2009，92，11： | 1 |
| 20 | Cyclooxygenase-2 mediated regulation of E-cadherin occurs in conventional but not early-onset gastric cancer cell lines显示文摘 | Sitarz R Leguit R J de Leng WW | 2009 | Cell Oncol2009，31，6： | 1 |
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