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| 1 | 最新AD研究用诊断标准:IWG-2标准显示文摘在过去的8年中,国际工作组织(IWG)和美国国立老化研究院-阿尔茨海默协会(NIA-AA)建立了阿尔茨海默病(AD)诊断标准,它能更好地定义AD的临床表型,整合了生物标记物于诊断流程中,并覆盖了疾病的全程。本意见书充分地权衡了IWG标准的优缺点,建议改进诊断框架。依据这些改进,AD的诊断变得简单,只要有恰当的AD临床表型(典型或不典型)和与AD的病理相一致的病理生理学生物标志物出现。我们认为疾病的下游的定位性生物标志,如容积性磁共振成像(MRI)和氟脱氧葡萄糖-正电子发射型计算机断层成像(FDG-PET)等,适合更好地测量和监测疾病过程。本文还详述了非典型性AD、混合性AD和AD临床前期的特异诊断标准。 | 陈刚 曹雯炜 俞羚 糜建华 Dubois B Feldman HH Jacova C Hampel H Molinuevo JL Blennow K DeK osky ST Gauthier S Selkoe D Bateman R Cappa S Crutch S Engelborghs S Frisoni GB Fox NC Galasko D Habert MO Jicha GA Nordberg A Pasquier F Rabinovici G Robert P Rowe C Salloway S Sarazin M Epelbaum S de Souza LC Vellas B Visser PJ Schneider L Stern Y Scheltens P Cummings JL | 2014 | 神经病学与神经康复学杂志2014，11，3： | 31 |
| 2 | 住院老年病人的非药物睡眠方法显示文摘 | Mcdo.,JA Mion.,LC | 1999 | 世界医学杂志1999，3，5： | 17 |
| 3 | Does autologous blood transfusion during liver transplantation for hepatocellular carcinoma increase risk of recurrence?显示文摘AIM: To analyze outcomes in patients who underwent liver transplantation(LT) for hepatocellular carcinoma(HCC) and received autologous intraoperative blood salvage(IBS). METHODS: Consecutive HCC patients who underwent LT were studied retrospectively and analyzed according to the use of IBS or not. Demographic and surgical data were collected from a departmental prospective maintained database. Statistical analyses were performed using the Fisher's exact test and the Wilcoxon rank sum test to examine covariate differences between patients who underwent IBS and those who did not. Univariate and multivariate Cox regression models were developed to evaluate recurrence and death,and survival probabilities were estimated using the Kaplan-Meier method and compared by the log-rank test.RESULTS: Between 2002 and 2012,158 consecutive patients who underwent LT in the same medical center and by the same surgical team were identified. Among these patients,122(77.2%) were in the IBS group and 36(22.8%) in the non-IBS group. The overall survival(OS) and recurrence free survival(RFS) at 5 years were 59.7% and 83.3%,respectively. No differences in OS(P=0.51) or RFS(P=0.953) were detected between the IBS and non-IBS groups. On multivariate analysis for OS,degree of tumor differentiation remained as the only independent predictor. Regarding patients who received IBS,no differences were detected in OS or RFS(P=0.055 and P=0.512,respectively) according to the volume infused,even when outcomes at 90 d or longer were analyzed separately(P=0.518 for both outcomes).CONCLUSION: No differences in RFS or OS were detected according to IBS use. Trials addressing this question are justified and should be designed to detect small differences in long-term outcomes. | Raphael LC Araujo Carlos Andrés Pantanali Luciana Haddad Joel Avancini Rocha Filho Luiz Augusto Carneiro D’Albuquerque Wellington Andraus | 2016 | World Journal of Gastrointestinal Surgery2016，8，2： | 14 |
| 4 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 5 | 非诺贝特治疗与2型糖尿病患者长期心血管病风险的关系显示文摘2型糖尿病患者心血管病风险高,部分原因在于高三酰甘油和低高密度脂蛋白胆固醇血症。而在他汀类药物治疗基础上,加强降低三酰甘油治疗,能否降低这种风险尚不清楚。该研究的目的是在使用他汀类药物治疗的2型糖尿病患者中,确定非诺贝特能否降低心血管病风险。 | 刘莉 叶鹏 Elam MB Ginsberg HN Lovato LC Corson M Largay J Leiter LA Lopez C O'Connor PJ Sweeney ME Weiss D Friedewald WT Buse JB Gerstein HC Probstfield J Grimm R Ismail-Beigi F Goff DC Jr Fleg JL Rosenberg Y Byington RP ACCORDION study investigators | 2017 | 中华高血压杂志2017，25，6： | 7 |
| 6 | 螺内酯与可乐定作为难治性高血压第4种治疗药物的比较:难治性高血压的最佳治疗随机研究显示文摘该研究旨在通过一个多中心随机试验对作为难治性高血压患者的第4种治疗药物的螺内酯与可乐定进行比较。采用药丸计数评估药物治疗依从性。难治性高血压患者(定义为包括1种利尿剂在内的3种药物治疗12周,诊室及动态血压监测血压仍未控制)随机给予螺内酯(12.5~50 mg/d)或可乐定(0.1~0.3mg,2次/d)继续治疗12周。 | Krieger EM Drager LF Giorgi DMA Pereira AC Barreto-Filho JAS Nogueira AR Mill JG Lotufo PA Amodeo C Batista MC Bodanese LC Carvalho ACC Castro I Chaves H Costa EAS Feitosa GS Franco RJS Fuchs FD Guimarāes AC Jardim PC Machado CA Magalhāes ME Mion D Jr Nascimento RM Nobre F Nóbrega AC Ribeiro ALP Rodrigues-Sobrinho CR Sanjuliani AF Teixeira MDCB Krieger JE ReHOT Investigators 赵狄 练桂丽 | 2018 | 中华高血压杂志2018，26，3： | 7 |
| 7 | 六氧甲基鸟嘌呤DNA甲基转移酶基因表达与肿瘤耐药显示文摘目的:六氧甲基鸟嘌呤DNA甲基转移酶(MGMT)能阻止由氮芥类导致的DNA交连形成,因而降低其细胞毒性。本研究探讨肿瘤细胞对氯乙基亚硝脲(CENU)耐药与其MGMT基因表达之间的关系。方法:采用RNA印迹试验和蛋白印迹试验对13株人肿瘤细胞的MGMT基因表达进行了检测,并与采用改良的SulforhodaminaB(SRB)比色抗癌药筛选法测定的肿瘤细胞对CENU的耐药性进行比较。结果:肿瘤细胞MGMT的表达在mRNA水平和蛋白水平是一致的。MGMT阳性的肿瘤比MGMT阴性的肿瘤对BCNU(t=2.611,P=0.01)和SarCNU(t=2.558,P=0.01)明显耐药。可是,MGMT表达高低与肿瘤细胞耐药性程度之间无线性相关。结论:MGMT在肿瘤细胞对CENU耐药中起重要作用,但除了MGMT之外,还有其它机制参与肿瘤耐药。 | 陈忠平 Mohr G Panasci LC 黄强 | 1998 | 癌症1998，17，3： | 6 |
| 8 | 妊娠期肝内胆汁淤积症女性使用熊去氧胆酸治疗与安慰剂比较:一项随机对照试验显示文摘【据《Lancet》2019 年9 月报道】题:妊娠期肝内胆汁淤积症女性使用熊去氧胆酸治疗与安慰剂比较:一项随机对照试验(作者Chappell LC 等)妊娠期肝内胆汁淤积症的特征是产妇瘙痒和血清胆汁酸浓度升高,与死产、早产和新生儿入院率增加有关。熊去氧胆酸是被广泛应用的治疗方法,但是没有充分的证据基础。 | 刘宇维 牛俊奇 CHAPPELL LC BELL JL SMITH A | 2020 | 临床肝胆病杂志2020，36，2： | 6 |
| 9 | Cyclic adenosine 3',5'-monophosphate induces differentiation of mouse embryonic stem cells into cardiomyocytes显示文摘 | Chen Y Shao JZ Xiang LX Guo J Zhou Q J Yao X Dai LC Lu YL | 2006 | 中国生物学文摘2006，20，7： | 5 |
| 10 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 11 | Effects of Heat Treatment on the Transformation Temperature and the Microstructure of Ni-Ti-Nb Shape Memory Alloy显示文摘The effects of heat treatment on the phase transformation temperature and the microstructure of Ni-Ti-Nb alloy have been investigated by means of R-T method, TEM and EPMA. With the increase of annealing temperature, cooling rate and ageing temperature, the Ms temperature increases. TEM observations show no new precipitated phase was found in the B2 parent phase after heat treatment, but EPMA shows the content of Ni (or Ni/Ti ratio) in the B2 matrix changed. The main reason for the change of transformation temperature lies in the change of the matrix composition (or Ni/Ti ratio) with the variation of heat treatment procedure. | Zheng, YF Cai, W Wang, YQ Luo, YC Zhao, LC | 1998 | Journal of Materials Science & Technology1998，14，1： | 4 |
| 12 | Effects of combination lipid therapy in type 2 diabetes mellitus显示文摘 | Ginsberg HN Elam MB Lovato LC | | New England Journal of Medicine0，，： | 4 |
| 13 | 唐氏综合征动物模型肠神经系发育的动态观察显示文摘目的通过研究唐氏综合征动物模型16三体鼠肠神经系发育,揭示16三体鼠是否伴有先天性巨结肠。方法通过免疫组织化学方法测定16三体胎鼠及同龄胎鼠蛋白基因产物9.5(PGP9.5),并观察了肠神经系的形态学特征。结果16三体胎鼠肠神经系发育异常,肠肌间神经丛发育迟缓,肠粘膜下神经丛缺如,盲肠末端有5mm的无神经节区。但肠道外源性神经支配正常,肠系膜神经系发育良好。结论唐氏综合征动物模型16三体鼠伴有先天性巨结肠,这种动物模型可用于研究先天性巨结肠病。 | 李继承 Busch,LC | 1999 | 中华医学杂志1999，79，6： | 3 |
| 14 | The development of colon innervation in trisomy mice and Hirschsprungs disease显示文摘AIM To study the colon innervation of trisomy16 mouse, an animal model for Downssyndrome, and the expression of protein geneproduct 9.5 ( PGP 9.5) in the stenosed segmentof colon in Hirschsprungs disease (HD).METHODS Trisomy 16 mouse breeding;cytogenetic analysis of trisomy 16 mice; andPGP 9.5 immunohistochemistry of colons oftrisomy 16 mice and HD were carried out.RESULTS Compared with their normalIittermates, the nervous system of colon intrisomy 16 mice was abnormally developed.There existed developmental delay of muscularplexuses of colon, no submucosal plexus wasfound in the colon, and there was 5mmaganglionic bowel aparting from the anus intrisomy 16 mice. The mesentery nerve fiberswere as well developed as shown in their normallittermates. Abundant proliferation of PGP 9.5positive nerve fibers was revealed in thestenosed segment of HD colon.CONCLUSION Trisomy 16 mice could serve asaganglionic bowel in the distal part of colon.Abundant proliferation of PGP 9.5 positive fibersresulted from extrinsic nerve compensation,since no ganglionic cells were observed in thestenosed segment of the colon in HD. HD has agenetic tendency. | LC Busch W Kuhnel | 2001 | World Journal of Gastroenterology2001，7，1： | 3 |
| 15 | Could the burden of pancreatic cancer originate in childhood?显示文摘The presence of pancreatic cancer during childhood is extremely rare,and physicians may be tempted to overlook this diagnosis based on age criteria.However,there are primary malignant pancreatic tumors encountered in pediatric patients,such as pancreatoblastoma,and tumors considered benign in general but may present a malignant potential,such as the solid pseudo-papillary tumor,insulinoma,gastrinoma,and vasoactive intestinal peptide secreting tumor.Their early diagnosis and management are of paramount importance since the survival rates tend to differ for various types of these conditions.Many pediatric cancers may present pancreatic metastases,such as renal cell carcinoma,which may evolve with pancreatic metastatic disease even after two or more decades.Several childhood diseases may create a predisposition for the development of pancreatic cancer during adulthood;hence,there is a need for extensive screening strategies and complex programs to facilitate the transition from pediatric to adult healthcare.Nevertheless,genetic studies highlight the fact the specific gene mutations and family aggregations may be correlated with a special predisposition towards pancreatic cancer.This review aims to report the main pancreatic cancers diagnosed during childhood,the most important childhood diseases predisposing to the development of pancreatic malignancies,and the gene mutations associates with pancreatic malignant tumors. | Smaranda Diaconescu Georgiana Emmanuela Gîlcă-Blanariu Silvia Poamaneagra Otilia Marginean Gabriela Paduraru Gabriela Stefanescu | 2021 | World Journal of Gastroenterology2021，27，32： | 3 |
| 16 | 体质量状态变化与高血压发生的相关性显示文摘在美国近1/3的儿童和青少年是肥胖或超重的。虽然最近该比值保持稳定,但在过去40年中则呈快速上升的趋势。儿童肥胖与高血压显著相关。主要在初中和高中学生中进行的流行病学调查研究报道,高血压的发病率在0.8%~3.2%,而在肥胖学生中高血压发病率则〉10%。在大型的医疗保健系统门诊和小学儿童中进行的一项研究报道显示,正常体质量的儿童高血压总发病率为0.3%~0.9%,肥胖儿童为1.0%~9.2%。 | Parker ED Sinaiko AR Kharbanda EO Margolis KL Daley MF Trower NK Sherwood NE Greenspan LC Lo JC Magid DJ o'connor pj | 2016 | 中华高血压杂志2016，24，6： | 3 |
| 17 | Pushing the limits of liver surgery for colorectal liver metastases:Current state and future directions显示文摘Liver surgery for the treatment of colorectal liver metastases is the standard treatment in a dynamic surgical field with many variables that should be considered in a curative intent scenario. Hepatectomy for colorectal liver metastases has undergone constant changes over the last 30 years, including indications until the need for rescue procedures of recurrent and advanced diseases as well as minimally invasive surgery. These advancements in liver surgery have not only resulted from overall improvements in the surgical field but have also resulted from a better understanding of the biological behavior of the disease, liver regeneration, and homeostasis during and after surgery.Improvements in anesthesiology, intensive care medicine, radiology, and surgical devices have correlated with further advancements of hepatectomies. Moreover,changes are still forthcoming, and both fields of augmented reality and artificial intelligence will likely have future contributions in this field in regard to both diagnoses and the planning of procedures. The aim of this editorial is to emphasize several aspects that have contributed to the paradigm shifts in colorectal liver metastases surgery over the last three decades as well as to discuss the factors concerning patient selection and the technical aspects of liver surgery. Finally, this editorial will highlight the promising new features of this surgery for diagnoses and treatments in this field. | Raphael LC Araujo Marcelo M Linhares | 2019 | World Journal of Gastrointestinal Surgery2019，11，2： | 3 |
| 18 | 最优延迟时间显示文摘 | Lang,LC 宗琦 | 1991 | 煤炭科技资料1991，，3： | 3 |
| 19 | Cognitive disorder and dementia in type 2 diabetes mellitus显示文摘Insulin,a key pleiotropic hormone,regulates metabolism through several signaling pathways in target tissues including skeletal muscle,liver,and brain.In the brain,insulin modulates learning and memory,and impaired insulin signaling is associated with metabolic dysregulation and neurodegenerative diseases.At the receptor level,in aging and Alzheimer’s disease(AD)models,the amount of insulin receptors and their functions are decreased.Clinical and animal model studies suggest that memory improvements are due to changes in insulin levels.Furthermore,diabetes mellitus(DM)and insulin resistance are associated with age-related cognitive decline,increased levels ofβ-amyloid peptide,phosphorylation of tau protein;oxidative stress,pro-inflammatory cytokine production and dyslipidemia. Recent evidence shows that deleting brain insulin receptors leads to mildobesity and insulin resistance without influencing brain size and apoptosis development.Conversely, deleting insulin-like growth factor 1 receptor (IGF-1R) affects brain size anddevelopment, and contributes to behavior changes. Insulin is synthesized locally in the brain andis released from the neurons. Here, we reviewed proposed pathophysiological hypotheses toexplain increased risk of dementia in the presence of DM. Regardless of the exact sequence ofevents leading to neurodegeneration, there is strong evidence that mitochondrial dysfunctionplays a key role in AD and DM. A triple transgenic mouse model of AD showed mitochondrialdysfunction, oxidative stress, and loss of synaptic integrity. These alterations are comparable tothose induced in wild-type mice treated with sucrose, which is consistent with the proposal thatmitochondrial alterations are associated with DM and contribute to AD development. Alterationsin insulin/IGF-1 signaling in DM could lead to mitochondrial dysfunction and low antioxidantcapacity of the cell. Thus, insulin/IGF-1 signaling is important for increased neural processing andsystemic metabolism, and could be a specific target for therapeutic strategies to decreasealterations associated with age-related cognitive decline. | Genaro G Ortiz Miguel Huerta Héctor A González-Usigli Erandis D Torres-Sánchez Daniela LC Delgado-Lara Fermín P Pacheco-Moisés Mario A Mireles-Ramírez Blanca MG Torres-Mendoza Roxana I Moreno-Cih Irma E Velázquez-Brizuela | 2022 | World Journal of Diabetes2022，13，4： | 2 |
| 20 | 常规血液储存中红细胞膜的氧化损伤显示文摘为了保持输血后红细胞(RBC)在循环中的存活率,经多年研究建立了一种比较有效的方法,就是在保养液中加入一定浓度的ATP.虽然成功地储存了RBC,但也有许多资料证实了RBC仍然要受到损伤.其原因可能是操作技术、创伤或非自然损伤等人为因素所致,因而建立了为避免RBC损伤的一些措施,RBC不仅能储存在4℃条件下,而且输入人体后能存活数天至七周之久.要使储存后RBC的存活率不断得到改善,需要进一步阐明正常和异常RBC的结构和功能,为今后储存RBC时作参考.红细胞膜与氧化损伤的关系由于开展了对盘状棘形RBC的转化过程与ATP耗竭的早期研究。 | Woife LC 杨先军 | 1990 | 国外医学(输血及血液学分册)1990，13，5： | 2 |
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