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| 1 | Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R显示文摘AIM: To investigate the correlation between subcellulardaunorubicin distribution and the multidrug resistancephenotype in drug-resistant cell line SMMC-7721/R.METHODS: The multidrug resistant cell line SMMC-7721/R, a human hepatocellular carcinoma cell line,was established. Antisense oligonudeotides (AS-ODN)were used to obtain different multidrug resistancephenotypes by inhibiting the expression of mdr1 geneand/or multidrug resistance-related protein gene(mrp)using Lipofectamine as delivery agent. Expression ofmdr1 and mrp genes was evaluated by RT-PCR andWestern blotting. Intracellular daunorubicin (DNR)concentration was measured by flow cytometry.Subcellular DNR distribution was analyzed by confocallaser scanning microscopy. Adriamycin (ADM) and DMRsensitivity was examined by MTT method.RESULTS: Low level expression of mdr1 and mrp mRNAsand no expression of P-Glycoprotein(P-gp) andmultidrug resistance-related protein (P190) weredetected in parental sensitive cells SMMC-7721/S, butover-expression of these two genes was observed indrug-resistant cell SMMC-7721/R. The expression ofmdr1 and mrp genes in SMMC-7721/R cells was downregulated to the level in the SMMC-7721/S cells by AS-ODN. Intracellular DNR concentration in SMMC-7721/S cells was 10 times higher than that in SMMC-7721/Rcells. In SMMC7721/S cells intracellular DNRdistributed evenly in the nucleus and cytoplasm, whilein SMMC-7721/R cells DNR distributed in a punctatepattern in the cytoplasm and was reduced in thenucleus. DNR concentration in SMMC-7721/R cells co-transfected with AS-ODNs targeting to mdr1 and mrpmRNAs recovered to 25 percent of that in SMMC7721/Scells. Intracellular DNR distribution pattern in drug-resistant cells treated by AS-ODN was similar to drug-sensitive cell, and the cells resistance index (RI) to DNRand ADM decreased at most from 88.0 and 116.0 to 4.0and 2.3, respectively. Co-Transfection of two AS-ODNsshowed a stronger synergistic effect than separatetransfection.CONCLUSIONS: P-gp and P190 are two membersmediating MDR in cell line SMMC7721/R. Intracellulardrug concentration increase and subcellular distributionchange are two important factors in multidrugresistance (MDR) formation. The second factor, drugstransport by P-gp and P190 from cell nucleus to organellin cytoplasm, may play a more important role. | Jia-Yin Yang Hua-You Luo Qi-Yuan Lin Zi-Ming Liu Lu-Nan Yan Department of General Surgery,West China Hospital,Sichuan University,Chengdu 610044,Sichuan Province,China Ping Lin Cancer Research Institution,West China Hospital,Sichuan University,610044,Sichuan Province,China Jie Zhang Department of Confocal Laser Scanning Microscopy,West China Hospital,Sichuan University,610044,Sichuan Province,China Shong Lei Cancer Biotechnological Treatment Center,West China Hospital,Sichuan University,610044,Sichuan Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Processing technique of target capsule's micro inflation hole with a scanning probe显示文摘To resolve inflation of inertial confinement fusion (ICF) target and encapsulation of micro inflation hole with adhesive, a scanning probe microscope (SPM) diamond microprobe was used as the cutting tool with SPM in contact mode. Some parameters influencing the quality of micro inflation hole, such as the scanning direction of the diamond tip, the scanning rate and the contact force are discussed. Accurate taper hole was achieved whose dimension and precision could meet the requirements of inflation and encapsulation technique of micro hole. The experimental results show that using SPM diamond microprobe as the cutting tool and with special processing technique, the precision machining of target capsule's taper inflation hole can be realized. A novel operative technology for filling high Z gas to target is provided. | SUN Tao, YAN Yongda, GAO Dangzhong, TANG Yongjian, FU Yibei & DONG ShenPrecision Engineering Research Institute, Harbin Institute of Technology, Harbin 150001, China Research Centre of Laser Fusion, Chinese Academy of Engineering Plysics, Mianyang 621900, China Institute of Nuclear Physics and Chemistry, Chinese Academy of Engineering Physics, Mianyang 621900, China | 2004 | Science China(Technological Sciences)2004，47，1： | 7 |
| 3 | Research on EM pulse protection property of plasma-microwave absorptive material-plasma sandwich structure显示文摘A plasma-microwave absorptive material (MAM)-plasma sandwich structure is presented to protect the electronic device against high power electromagnetic pulse. The model of electromagnetic wave reflected by and transmitting through the structure is established. Based on the characteristic parameters of plasma generated by discharge and usual MAM, the electromagnetic transmissive properties of the sandwich structure are investigated by the method of finite difference in time domain. The results indicate that in a rather broad frequency range, the electromagnetic attenuations by the structure are obviously better than the sum of attenuations resulted from plasma and MAM respectively. The models and results presented are instructive for electromagnetic pulse protection. | YUAN ZhongCai & SHI JiaMing State Key Laboratory of Pulse Power Laser Technology, Key Lab of Infrared and Low Temperature Plasma of Anhui Province, Hefei Electronic Engineering Institute, Hefei 230037, China | 2010 | Science China(Technological Sciences)2010，53，12： | 6 |
| 4 | High sensitive detection of presenilin-1 point mutation based on electrochemilumi-nescence显示文摘Electrochemiluminescence (ECL) is a high- sensitive detection method with broad biological applications. Ruthenium (Ⅱ) tris (bipyridyl) 2+3(Ru(bpy)) and tripro-pylamine (TPA) are most commomly used combination of the reactive species in ECL. The redox of 2+3Ru(bpy) with excess TPA at the surface of an electrode produces a highly efficient and stable light emission due to a rapid cycle of the reactions. This rapid, highly sensitive and accurate method has been applied to gene quantification. In this work, an ECL detection system is designed and applied in detection of presenilin-1 (PS-1) gene mutation. The results show that given the same polymerase chain reaction (PCR) cycle num-ber, the ECL intensities from the digested and the undigested wild-type samples are nearly identical, but the difference in ECL intensities between the digested and the undigested mutant samples is distinctive. This detection technology connects PCR with ECL and allows a reliable discrimination between the wild-type and the mutant genes. | ZHU Debin, XING Da, SHEN Xingyan & YAN Guihong Institute of Laser Life Science, South China Normal University, Guang-zhou 510631, China Correspondence should be addressed to Xing Da (e-mail: xingda@ scnu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，16： | 4 |
| 5 | CALCULATION OF 3-D TRANSIENT TEMPERATURE FIELD DURING LASER TRANSFORMATION HARDENING PROCESS显示文摘A three-dimensional transient heat transfer model for laser transformation hardening process has been developed in this paper. The finite size of the laser treated sample, the surface heat loss of the sample, the latent heat of phase transformation and the temperature dependence of thermal properties of materials were considered. The heat source was considered as a moving Gaussian heat flux with a constant velocity. Three-dimension unequally spatial grid explicit finite difference equations, alternating direction implicit finite difference equations and implicit finite difference equations were deduced respectively. Three programs to calculate the temperature field were developed using Fortran language. The transient temperature fields of C22, 42CrMo, C60 steel samples during laser transformation hardening process were calculated using these programs, and the widths and depths of laser transformation hardening zones were also predicted. C22, 42CrMo, C60 steel samples were treated by CO2 laser,the widths and depths of laser transformation hardening zones of these samples were also measured experimentally. The calculated widths and depths of laser transformation hardening zones are in good agreement with the experimental results. | L. W Zhang, R.S. Wang, J. Th.M.De Hosson, Y.L. Xia and F. G. Wang 1) The State Key Lab. for Materials Modification by Laser, Ion and Electron Beams, Dalian University of Technology, Dalian 116023, China 2) Department of Applied Physics, University of Gro | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，2： | 4 |
| 6 | A mechanism for enhanced photocatalytic activity of nano-size silver particle modified titanium dioxide thin films显示文摘Ag-TiO2 nanostructured thin films with silver volume fraction of 0–20% were deposited on silicon and quartz substrates by RF magnetron sputtering and annealed in ambient air at 950°C for 1 h. The phase structure and surface topography of the films were characterized by X-ray diffractometer and transmission electron microscope. Photocatalytic activity of the films was evaluated by light induced degradation of methyl orange (C14H14N3NaO3S) solution using a high pressure mercury lamp as lamp-house. The relation of photocatalytic activity and silver content was studied in detail. It was found that silver content influences phase structure of TiO2 thin films, and silver in the films is metallic Ag (Ag0). With increasing silver content from 0 to 20 vol%, photocatalytic activity of the films increases first and then decreases. A suitable amount (2.5–5 vol%) silver addition can significantly enhance the photocatalytic activity of TiO2 films. The enhanced photocatalytic activity was mainly attributed to the extension of visible light absorption region of the films, the presence of anatase phase, the increase of oxy-gen anion radicals O2? and reactive center of surface Ti3+, and the better separation between electrons and holes on the films surface. | MENG FanMing1,2,3*, LU Fei1,3, SUN ZhaoQi1,3 & Lü JianGuo1,2,4 1 School of Physics and Materials Science, Anhui University, Hefei 230039, China 2 Key Laboratory of Materials Modification by Laser, Ion and Electron Beams (Dalian University of Technology), Ministry of Education, Dalian 116024, China 3 Anhui Key Laboratory of Information Materials and Devices, Anhui University, Hefei 230039, China 4 Department of Physics and Electronic Engineering, Hefei Normal University, Hefei 230061, China | 2010 | Science China(Technological Sciences)2010，53，11： | 4 |
| 7 | Performance Comparison and Analysis of IP and MPLS Networks显示文摘With the increasing need for Quality of Service( QoS ),the traditional IP network is becoming less competitive. In order to give readers quantitative and convictive performance comparisons between IP and MPLS network,we choose Berkeleys NS 2 simulator [1] in our research on network throughput and end to end TCP behavior. Using NS 2 simulator,we analyze the performance of a network with MPLS Traffic Engineering( MPLS TE ).Specifically,the throughput and TCP end to end delay time under two different queuing algorithms are obtained and compared between the MPLS network and traditional IP network. We find that in the IP network using SFQ queuing algorithm will help traffic balance compared to the simple FIFO scheme,but it still can not utilize the available bandwidth,which in turn leads to the poor performance as in the FIFO case.With MPLS TE , the throughput will be higher and the end to end TCP delay time remains almost the same even in heavy load case.We notice that if flows are isolated partially,using SFQ scheme will have a comparable performance as the fully isolation case using FIFO scheme in a MPLS network,and that if flows are isolated fully for FIFO and SFQ schemes,both will have the same result.Finally,we conclude that a better queuing algorithm will help reduce the label space requirement. | LIU Er wu, CAO Ming cui, CHEN Chun han, LI Feng (State Key Laboratory of Laser Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R.China) | 2001 | The Journal of China Universities of Posts and Telecommunications2001，8，2： | 3 |
| 8 | A systematic investigation of high harmonic generation using mid-infrared driving laser pulses显示文摘We report on a systematic investigation of the influences of gas pressure,focal position and focusing geometry on high harmonic generation by use of mid-infrared femtosecond laser pulses. We also discuss the spatial characteristics of harmonics under different focusing conditions. By optimizing the parameters,we experimentally observed the generation of 1 kHz,low divergence coherent X-ray beams in the water-window region. | YAO JinPing 1,2 ,XIONG Hui 1 ,XU Han 1 ,FU YuXi 1,2 ,ZENG Bin 1,2 ,CHU Wei 1,2 ,CHENG Ya 1 ,XU ZhiZhan 1 ,LIU XiaoJun 3 & CHEN Jing 4,5 1 State Key Laboratory of High Field Laser Physics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences,Wuhan 430071,China 4 Institute of Applied Physics and Computational Mathematics,Beijing 100088,China 5 Center for Applied Physics and Technology,Peking University,Beijing 100084,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，6： | 3 |
| 9 | Calculation of Temperature Field During Laser Transformation Hardening of Cylindrical Bodies显示文摘A Two-dimensional transient heat transfer model for laser transformation hardening process of cylindrical bodies has been developed. The surface heat loss of the sample, the latent heat of phase transformation, and the temperature dependence of thermal properties of material were considered. The laser heat source was considered as a moving Gaussian heat flux with a constant velocity. Finite element method was used to calculate the transient temperature field. A program to calculate the temperature field was developed using FORTRAN language. The transient temperature field, the width and depth of laser transformation hardening zone of 42CrMo steel sample during laser transformation hardening process was calculated. The widths and depths of laser transformation hardening zones of 42CrMo steel samples were also measured experimentally. The calculated widths and depths of laser transformation hardening zones are in good agreement with the experimental results. | Liwen Zhang, Lixia Wei Guoliang Zhang, J. Th.MDe Hosson Yuanliang Xia , Fugang Wang 1.The State Key Laboratory for Materials Modification by Laser, Ion and Electron Beams 2.Material Engineering Department of Dalian University of Technology, Dalian 11 60 | 2000 | Journal of Shanghai Jiaotong university(Science)2000，5，1： | 3 |
| 10 | The characteristics of arc beam shaping in hybrid plasma and laser deposition manufacturing显示文摘As a new direct metal prototyping technology,the hybrid plasma and laser deposition manufacturing (PLDM) is proposed in this paper. In order to figure out the characteristics of plasma arc beam and mould in the PLDM process of high temperature alloy, the high speed CCD camera is used to obtain the picture around the plasma arc. Afterwards the sketch of picture is clearly obtained. And the effect of laser parameter, such as average power, pulse width, pulse repetition frequency and the angle between laser beam and plasma arc beam on the plasma arc appearance, is studied experimentally. The results show that the modality of plasma arc beam is markedly influenced by laser beam. And the improvements of shape precision and surface state of the layer deposited by PLDM are confirmed. | ZHANG Hai’ou1, QIAN Yingping1,3, WANG Guilan1 & ZHENG Qiguang2 1. School of Material Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China 2. Institute of Laser Technology and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China 3. Mechanical Engineering College, Hubei University of Technology, Wuhan 430068, China | 2006 | Science China(Technological Sciences)2006，49，2： | 2 |
| 11 | A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol显示文摘 | Mark Laser Deborah Schulman Stephen G Allen Joseph Lichwa Michael J Antal Lee R Lynd | 2001 | Bioresource Technology2001，，1： | 2 |
| 12 | I-V and C-V properties of TiO_2 thin film by pulsed-laser reactive deposition显示文摘TiO-2 thin films have bee deposited on p-Si(111) substrates by pulsed-laser ablation of metallic Ti target in the O-3 ambient. The current-voltage and capacitance-voltage of the Al/TiO-2/Si capacitors are measured. The results show that the dielectric constant of thin film after being annealed at 700℃ is found to be 46, and the border trap density and the interface state density at the TiO-2/p-Si interface are 1.8×10 12 cm -2 and 2×10 12 eV -1·cm -2, respectively. The conduction mechanisms of as-deposited films are also discussed. | FU Zhengwen 1, ZHOU Mingfei 1, ZHANG Shengkun 2,CHEN Lianyao 2 and QIN Qizong 1 1. Laser Chemistry Institute, Fudan University, Shanghai 200433, China 2. Department of Physics, Fudan University, Shanghai 200433, China | 1998 | Chinese Science Bulletin1998，43，16： | 2 |
| 13 | Online diagnosis of electron excitation temperature in CH_4+H_2 discharge plasma at atmospheric pressure by optical emission spectra显示文摘Methane coupling under low temperature plasmas at atmospheric pressure is a green process by use of renewable sources of energy. In this study, CH4+H2 dis- charge plasma was on-line diagnosed by optical emission spectra so as to char- acterize the discharge system and to do spade work for the optimization of the technical parameters for future commercial production of methane coupling under plasmas. The study was focused on a calculation method for the online diagnosis of the electron excitation temperature in CH4+H2 discharge plasma at atmospheric pressure. The diagnostic method is easy, efficient and fairly precise. A serious er- ror in a literature was corrected during the reasoning of its series of equations formerly used to calculate electron temperatures in plasmas. | CUI JinHua1, XU ZhenFeng2, ZHANG JiaLiang3↑, NIE QiuYue3, XU GenHui4 & REN LongLiang5 1 Faculty of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116021, China 2 Technique Center for Modern Education, Liaodong College, Dandong 126709, China 3 State Key Laboratory for Materials Modification by Laser, Ion and Electron Beams, Dalian University of Technology, Dalian 116023, China 4 College of Chemical Engineering, Tianjin University, Tianjin 300072, China 5 Department of Physics, Science College, Tianjin University, Tianjin 300072, China | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，12： | 2 |
| 14 | The Beam Propagation Factor M^2 of Fiber Cross-Section Diffraction显示文摘Based on non-paraxial scalar theory, the beam quality factor M2 of LR propagating mode is studied. The result shows M2 factor is greater than 1 and it increase as A increases. | Wang Ke, Guo Fuyuan, Lin BinCNERC for Optical Instrument, State Key Lab of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, P. R. China, Tel: 86-571-87951213, Fax: 86-571-87951214,E-mail: wangke79@sina.com Institute of Laser, Fujian Normal University, P. R. China, Tel. and Fax: 86-591-3465373, E-mail:guofy@zju.edu.cn | 2003 | 光学学报2003，23，S1： | 2 |
| 15 | Influence of Nitrogen Flow Rate on the Microstructure and Properties of N and Me (Me=Cr,Zr) Co-doped Diamond-like Carbon Films显示文摘In this paper,Me (Me=Cr,Zr) and N co-doped diamond like carbon (DLC-MeN) composite films were prepared on cemented carbide substrates by pulsed bias arc ion plating.The effect of nitrogen flow rates on the microstructure and properties of the films were investigated by X-ray photoelectron spectroscopy (XPS),Raman spectra,grazing incident X-ray diffraction (GIXRD),high resolution transmission electron microscopy (HRTEM) and nano-indentation.Raman,GIXRD and HRTEM results show that the deposited films are nanocomposite films with MeN nanocrystalline phase embedded within DLC amorphous matrix,which are vital for the mechanical properties of the films.The nitrogen flow rate has significant effect on the compositions and structures and hence on the hardness and elastic modulus of the films,and increasing nitrogen flow rate decreases drastically the hardness and elastic modulus of the films. | Zhiyun Han,Hongkai Li,Guoqiang Lin and Chuang Dong Key Laboratory for Material Modification by Laser,Ion and Electron Beams,Dalian University of Technology,Dalian 116085,China | 2010 | Journal of Materials Science & Technology2010，26，11： | 2 |
| 16 | Automated Phase-measuring Profilometry of 3-D Diffuse Objects Using a Single Phase Step Method显示文摘AutomatedPhase-measuringProfilometryof3-DDifuseObjectsUsingaSinglePhaseStepMethod①ZHAOHong,SONGYuanhe,CHENWenyi,TANYushan(Res... | ZHAO Hong,SONG Yuanhe,CHEN Wenyi,TAN Yushan (Research Institute for Laser & Infrared Application, Xi’an Jiaotong University,Xi’an 710049,CHN) | 1998 | Semiconductor Photonics and Technology1998，4，1： | 2 |
| 17 | LASER GLAZING OF Ni-Nb AND Ni-Nb-Cr ALLOYS AND CORROSION RESISTANCE OF AMORPHOUS LAYERS显示文摘Partially amorphous Ni-Nb alloy was made by 1.91 kW cw CO2 laser melt-quenching,and fully amorphous by 4.5 kW and 4.9 kW.The corrosion resistance of the amor-phous layer was improved by adding Cr.Potentiodynamic polarization curves showthat the amorphous Ni50Nb40Cr10 is in more corrosion-resistant than the amorphousNi60Nb40and 18-8 stainless steel. | YAN Yuhe YANG Yongqiang ZHAO Changming Tianjin Institute of Textile Science and Technology,Tianjin,ChinaWU Weitao WANG Maocai Institute of Corrosion and Protection of Metals,Academia Sinica,Shenyang,ChinaLU Mengfu Tianjin Institute of Science and Technology,Tianjin,China Lecturer,Laser Processing Lab.,Tianjin Institute of Textile Science and Technology,Tianjin 300160,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，7： | 1 |
| 18 | 36kW continuous wave output power 显示文摘 | JEONG Y SAHU J K PAYNE D N Ytterbium-doped large-core fiber laser with 1 | 2004 | Optics Express2004，12，25： | 1 |
| 19 | Anatomy and cytology of microsporogenesis in cytoplasmic male sterile angiosperms显示文摘 | Kenneth D. Laser Nels R. Lersten | 1972 | The Botanical Review1972，，3： | 1 |
| 20 | Creatinine and Maillard reaction products as precursors of muta- genic compounds formed in fried beef显示文摘 | Jagerstad M Laser Reutersward A Oste R | 1983 | ACS Symposium series-American Chemical Society (US- A)1983，12，4： | 1 |
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