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| 1 | Construction of Streptomyces lydicus A01 transformant with the chit33 gene from Trichoderma harzianum CECT2413 and its biocontrol effect on Fusaria显示文摘Streptomyces lydicus A01 resists many plant pathogens (including Fusarium spp.) by producing the antifungal agent natamycin, which binds to the ergosterol of fungal cell membranes and inhibits the growth of pathogens. Trichoderma harzianum CECT2413 is a widely-distributed soil fungus that antagonizes several plant fungal pathogens (including Fusarium spp.) by producing chi-tinase and degrading chitin, a major component of the fungal cell wall. This study attempted to enhance the biocontrol effect of S. lydicus A01 on Fusarium spp. by transforming the chitinase gene of Trichoderma. Chitinase and natamycin could act synergisti-cally on both the cell walls and cell membranes of pathogens. The 33-kD chitinase-encoding gene (chit33) was cloned and conju-gal-transformed from T. harzianum CECT2413 to S. lydicus A01, and then confirmed via polymerase chain reaction (PCR) assays. Subsequent analyses using the 3,5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry showed that compared with its wild type strain (WT), the S. lydicus A01 conjugal transformant (CT) with chit33 gene exhibited substantially higher chi-tinase activity and natamycin production. The resistance of S. lydicus A01-chit33 CT and WT to four Fusaria in crops and vegetables was tested via the cup-plate method. Compared with the WT, the conjugal transformant of S. lydicus A01 with chit33 gene from T. harzianum CECT2413 showed greatly increased biocontrol effect on fusarium disease. This study would be beneficial to the development of high-quality antifungal bio-agents for agricultural applications via the synergy between the previously non-existent and pre-existing functions achieved through heterogeneous gene transformation. | WU Qiong BAI LinQuan LIU WeiCheng LI YingYing LU CaiGe LI YaQian LIN ZhenYa WANG Meng XUE ChunSheng CHEN Jie | 2013 | Chinese Science Bulletin2013，58，26： | 1 |
| 2 | Spatial Identities of Public Health Service Facilities in Metropolitan Suburban Communities: A Case Study of Shanghai Jinshan District显示文摘Both 'Healthy China' and 'Healthy Shanghai' programs require construction and development of community public health services. Taking Jinshan District in Shanghai as a case study, this paper evaluates the status quo of community health services from the 'demand-supply' perspective, and finds a remarkable difference in the needs among different age groups, a big gap between urban and rural areas in the coverage degree of facilities, and different challenges for public service at the 'prevention – treatment – health care' stages. It further puts forward several planning strategies involving(1) specific service supply based on different age groups,(2) '15-minute health service cycles' in both urban and rural communities,(3) adjustment of facilities to local conditions, and(4) promoting the quality of health services. Furthermore, it discusses in-depth issues underlying planning policies, such as the innovation of service content, the integration of different plans, and the cultivation of health service needs. | Liu Bo Shen Li Lu Wei Li Caige | 2018 | China City Planning Review2018，27，1： | 1 |
| 3 | Streptomyces lydicus A_(01) affects soil microbial diversity, improving growth and resilience in tomato显示文摘The actinomycete Streptomyces lydicus A_(01) promotes tomato seedling growth; however, the underlying mechanism is unclear. In this study, we investigated whether changes in soil microbial diversity, following Streptomyces lydicus A_(01) treatment, were responsible for the increased tomato seedling growth. Eukaryotic 18 S ribosomal DNA(rDNA) sequencing showed that S. lydicus A_(01)-treated and untreated soil shared 193 operational taxonomic units(OTUs), whereas bacterial 16 S rDNA sequencing identified 1,219 shared OTUs between the treated and untreated soil. Of the 42 dominant eukaryotic OTUs, eight were significantly increased and six were significantly decreased after A_(01) treatment. Of the 25 dominant bacterial OTUs, 12 were significantly increased and eight were significantly decreased after A_(01) treatment.Most of the eukaryotes and bacteria that increased in abundance exhibited growth promoting characteristics,which were mainly predicted to be associated with mineralization of nitrogen and phosphorus, phosphate solubilization, nutrient accumulation, and secretion of auxin, whereas some were related to plant protection,such as the degradation of toxic and hazardous substances. Soil composition tests showed that S. lydicus A_(01) treatment enhanced the utilization of nitrogen, phosphorus, and potassium in tomato seedlings. Thus, microbial fertilizers based on S. lydicus A_(01) may improve plant growth, without the detriment effects of chemical fertilizers. | Qiong Wu Caige Lu Mi Ni Hongli Wang Weicheng Liu Jie Chen | 2019 | Journal of Integrative Plant Biology2019，61，2： | 0 |
| 4 | Dihydroartemisinin increased the abundance of Akkermansia muciniphila by YAP1 depression that sensitizes hepatocellular carcinoma to anti-PD-1 immunotherapy显示文摘The effect of anti-programmed cell death 1(anti-PD-1)immunotherapy is limited in patients with hepatocellular carcinoma(HCC).Yes-associated protein 1(YAP1)expression increased in liver tumor cells in early HCC,and Akkermansia muciniphila abundance decreased in the colon.The response to anti-PD-1 treatment is associated with A.muciniphila abundance in many tumors.However,the interaction between A.muciniphila abundance and YAP1 expression remains unclear in HCC.Here,anti-PD-1 treatment decreased A.muciniphila abundance in the colon,but increased YAP1 expression in the tumor cells by mice with liver tumors in situ.Mechanistically,hepatocyte-specific Yap1 knockout(Yap1^(LKO))maintained bile acid homeostasis in the liver,resulting in an increased abundance of A.muciniphila in the colon.Yap1 knockout enhanced anti-PD-1 efficacy.Therefore,YAP1 inhibition is a potential target for increasing A.muciniphila abundance to promote anti-PD-1 efficacy in liver tumors.Dihydroartemisinin(DHA),acting as YAP1 inhibitor,increased A.muciniphila abundance to sensitize anti-PD-1 therapy.A.muciniphila by gavage increased the number and activation of CD8^(+)T cells in liver tumor niches during DHA treatment or combination with anti-PD-1.Our findings suggested that the combination anti-PD-1 with DHA is an effective strategy for liver tumor treatment. | Zhiqin Zhang Xinli Shi Jingmin Ji Yinglin Guo Qing Peng Liyuan Hao Yu Xue Yiwei Liu Caige Li Junlan Lu Kun Yu | 2023 | Frontiers of Medicine2023，17，4： | 0 |
| 5 | 国外城市规划会议专家谈:后疫情时代下的国土空间规划与城市升级显示文摘Introduction With the theme of'Territorial and Spatial Planning and City Upgrading in the Post-Pandemic Era,'the 2021 Annual Conference of the International Urban Planning Academic Committee of the Urban Planning Society of China(UPSC)was successfully held at Soochow University from May 29 to 30,2021.This conference aimed at sharing new thoughts and practices in China and other countries in the fields of territorial and spatial planning and coordinated development,urban resilience enhancement and green ecology sustainability. | 王凯 屠启宇 大卫·肖 Lu Xiaobei Shen Lei Li Zhigang Wang Chengkun Li Min(Translated) Qian Fang(Translated) Li Caige(Translated) Liang Sisi(Proofread) | 2022 | China City Planning Review2022，31，1： | 0 |
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